A ribosome-associating chaperone mediates GTP-driven vectorial folding of nascent eEF1A by Ibrahim M. Sabbarini, Dvir Reif, Kibum Park, Alexander J. McQuown, Anjali R. Nelliat, Charlotte Trejtnar, Volker Dötsch, Eugene I. Shakhnovich, Andrew W. Murray, Vladimir Denic

“…Abstract Eukaryotic translation elongation factor 1A (eEF1A) is a highly abundant, multi-domain GTPase. …”
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Exploring the Gating Mechanism of the Human Copper Transporter, hCtr1, Using EPR Spectroscopy by Shahaf Peleg, Shelly Meron, Yulia Shenberger, Lukas Hofmann, Lada Gevorkyan-Airapetov, Sharon Ruthstein

“…Ctr1 is a membrane-spanning homotrimer that facilitates copper uptake in eukaryotic cells with high affinity. While structural details of the transmembrane domain of human Ctr1 have been elucidated using X-ray crystallography and cryo-EM, the transfer mechanisms of copper and the conformational changes that control the gating mechanism remain poorly understood. …”
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The mechanism of discriminative aminoacylation by isoleucyl-tRNA synthetase based on wobble nucleotide recognition by Bingyi Chen, Fang Yi, Zhiteng Luo, Feihu Lu, Hongwei Liu, Siting Luo, Qiong Gu, Huihao Zhou

“…ScIleRS utilizes a eukaryotic/archaeal-specific arginine as the H-bond donor to recognize the common carbonyl group (H-bond acceptor) of various N34s of tRNAIle, which induces mutual structural adaptations between ScIleRS and tRNAIle to achieve a preferable editing state. …”
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High-Throughput Screening and Molecular Dynamics Simulation of Natural Products for the Identification of Anticancer Agents against MCM7 Protein by Xin Zhang, Hui Chen, Hui Lin, Ronglan Wen, Fan Yang

“…Minichromosome maintenance complex component 7 (MCM7) belongs to the minichromosome maintenance family that is necessary for the initiation of eukaryotic DNA replication. Overexpression of the MCM7 protein is linked to cellular proliferation and is accountable for critical malignancy in many cancers. …”
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A Ralstonia solanacearum type III effector alters the actin and microtubule cytoskeleton to promote bacterial virulence in plants. by Rachel Hiles, Abigail Rogers, Namrata Jaiswal, Weiwei Zhang, Jules Butchacas, Marcus V Merfa, Taylor Klass, Pragya Barua, Venkatesh P Thirumalaikumar, Jonathan M Jacobs, Christopher J Staiger, Matthew Helm, Anjali S Iyer-Pascuzzi

“…Cellular responses to biotic stress frequently involve signaling pathways that are conserved across eukaryotes. These pathways include the cytoskeleton, a proteinaceous network that senses external cues at the cell surface and signals to interior cellular components. …”
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getphylo: rapid and automatic generation of multi-locus phylogenetic trees by T. J. Booth, S. Shaw, P. Cruz-Morales, T. Weber

“…We also demonstrate the flexibility of getphylo across four case studies including bacterial and eukaryotic genomes, and biosynthetic gene clusters. …”
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Evaluation of controls, quality control assays, and protocol optimisations for PacBio HiFi sequencing on diverse and challenging samples by Iraad F. Bronner, Emma Dawson, Naomi Park, Olaf Piepenburg, Michael A. Quail

“…The Darwin Tree of Life (DToL) project aims to generate high-quality reference genomes for all eukaryotic organisms in Britain and Ireland. At the time of writing, PacBio HiFi reads are generated for all samples using the Sequel IIe systems by the Wellcome Sanger Institute’s Scientific Operations teams, however we expect lessons from this work to apply directly to the Revio system too, as core principles of SMRT sequencing remain the same. …”
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Methylated H3K4, a transcription-associated histone modification, is involved in the DNA damage response pathway. by David Faucher, Raymund J Wellinger

“…Eukaryotic genomes are associated with a number of proteins such as histones that constitute chromatin. …”
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Tracing the paths of modular evolution by quantifying rearrangement events of protein domains by Abdulbaki Coban, Erich Bornberg-Bauer, Carsten Kemena

“…Results Here we determine, at unprecedented completeness, the rates of fusion, fission, emergence and loss of domains in five eukaryotic clades (monocots, eudicots, fungi, insects, vertebrates). …”
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Identification of novel genes responsible for a pollen killer present in local natural populations of Arabidopsis thaliana. by Anthony Ricou, Matthieu Simon, Rémi Duflos, Marianne Azzopardi, Fabrice Roux, Françoise Budar, Christine Camilleri

“…They are widely distributed in eukaryotes and are important for understanding genome evolution and speciation. …”
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Yeast silent mating type loci form heterochromatic clusters through silencer protein-dependent long-range interactions. by Adriana Miele, Kerstin Bystricky, Job Dekker

“…The organization of eukaryotic genomes is characterized by the presence of distinct euchromatic and heterochromatic sub-nuclear compartments. …”
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Identifying optimal substrate classes of membrane transporters. by Andreas Denger, Volkhard Helms

“…Furthermore, we implemented a pipeline for creating training datasets of transmembrane transporters that cover a wide range of organisms, including plants, bacteria, mammals, and single-cell eukaryotes. For a dataset containing 98.1% of transporters from S. cerevisiae, the pipeline automatically reduced the number of functional annotations from 287 to 11 GO terms that could be classified with a median pairwise F1 score of 0.87±0.16. …”
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Stalled disomes marked by Hel2-dependent ubiquitin chains undergo Ubp2/Ubp3-mediated deubiquitination upon translational run-off by Mario Scazzari, Ying Zhang, Anna Moddemann, Sabine Rospert

“…Abstract Stalled ribosomes cause collisions, impair protein synthesis, and generate potentially harmful truncated polypeptides. Eukaryotic cells utilize the ribosome-associated quality control (RQC) and no-go mRNA decay (NGD) pathways to resolve these problems. …”
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Replication timing in Drosophila and its peculiarities in polytene chromosomes by T. D. Kolesnikova, O. V. Antonenko, I. V. Makunin

“…Comparison of genomic replication profiles revealed a significant similarity between Drosophila and other well-studi ed eukaryotic species, such as human. Early replication is often confined to intensely transcribed gene-dense regions characterized by multiple replication initiation sites. …”
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Intracellular Membrane Contact Sites in Skeletal Muscle Cells by Matteo Serano, Stefano Perni, Enrico Pierantozzi, Annunziatina Laurino, Vincenzo Sorrentino, Daniela Rossi

“…Intracellular organelles are common to eukaryotic cells and provide physical support for the assembly of specialized compartments. …”
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Research progress on the antiviral immune regulation of ubiquitin ligase F-box family by MENG Xiangbo, LI Tianqi, ZHANG Tong

“…The F-box protein (FBP) family is a large and diverse protein family that is present in all eukaryotes. Based on the secondary structure of the C-terminal, FBPs can be classified as FBXW, FBXL, and FBXO. …”
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Variation in nuclear genome size within the Eisenia nordenskioldi complex (Lumbricidae, Annelida) by S. V. Shekhovtsov, Ya. R. Efremov, T. V. Poluboyarova, S. E. Peltek

“…The size of the nuclear genome in eukaryotes is mostly determined by mobile elements and noncoding sequences and may vary within wide limits. …”
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A bird’s-eye view of the biological mechanism and machine learning prediction approaches for cell-penetrating peptides by Maduravani Ramasundaram, Honglae Sohn, Thirumurthy Madhavan

“…Cell-penetrating peptides (CPPs) are highly effective at passing through eukaryotic membranes with various cargo molecules, like drugs, proteins, nucleic acids, and nanoparticles, without causing significant harm. …”
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Estimation of translational importance of mammalian mRNA nucleotide sequence characteristics based on ribosome profiling data by O. A. Volkova, Yu. V. Kondrakhin, R. N. Sharipov

“…Thanks to ribosome profiling technology, genome-wide experimental data of translation efficiency have been obtained for many eukaryotic mRNAs. Now it seems to be possible to reveal translationally important mRNA parameters and predict translation efficiency based on their nucleotide sequences. …”
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Identification of proteins that can participate in the recruitment of Ttk69 to genomic sites of Drosophila melanogaster by I. S. Osadchiy, T. N. Fedorova, P. G. Georgiev, O. G. Maksimenko

“…Interestingly, BTB-containing proteins are widely distributed only among higher eukaryotes. Many BTB-containing proteins are transcriptional factors involved in a wide range of developmental processes. …”
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