Repeated social defeat in male mice induced unique RNA profiles in projection neurons from the amygdala to the hippocampus by Rebecca G. Biltz, Wenyuan Yin, Ethan J. Goodman, Lynde M. Wangler, Amara C. Davis, Braedan T. Oliver, Jonathan P. Godbout, John F. Sheridan

“…This induced expression of a hemagglutinin epitope in neurons that project into the hippocampus. …”
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Preclinical model for evaluating human TCRs against chimeric syngeneic tumors by Olivier Michielin, Philippe Guillaume, Melita Irving, Patrick Reichenbach, Vincent Zoete, Elise Gray-Gaillard, Evangelos Stefanidis, Aikaterini Semilietof, Julien Pujol, Alessia D'Esposito

“…Background The adoptive cell transfer (ACT) of T cell receptor (TCR)-engineered T cells targeting the HLA-A2-restricted epitope NY-ESO-1157-165 (A2/NY) has yielded important clinical responses against several cancers. …”
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TCR transgenic clone selection guided by immune receptor analysis and single-cell RNA expression of polyclonal responders by Nincy Debeuf, Sahine Lameire, Manon Vanheerswynghels, Julie Deckers, Caroline De Wolf, Wendy Toussaint, Rein Verbeke, Kevin Verstaen, Hamida Hammad, Stijn Vanhee, Bart N Lambrecht

“…Here, we exploited the rapid advances in single-cell sequencing and TCR repertoire analysis to select the best clones without hybridoma selection, and generated CORSET8 mice (CORona Spike Epitope specific CD8 T cell), carrying a TCR specific for the Spike protein of SARS-CoV-2. …”
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Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross-stage recognition of glutamate-rich repeats by Axelle Amen, Randy Yoo, Amanda Fabra-García, Judith Bolscher, William JR Stone, Isabelle Bally, Sebastián Dergan-Dylon, Iga Kucharska, Roos M de Jong, Marloes de Bruijni, Teun Bousema, C Richter King, Randall S MacGill, Robert W Sauerwein, Jean-Philippe Julien, Pascal Poignard, Matthijs M Jore

“…A crystal structure of two B1E11K Fab domains in complex with its main antigen, RESA, expressed on asexual blood stages, showed binding of B1E11K to a repeating epitope motif in a head-to-head conformation engaging in affinity-matured homotypic interactions. …”
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Borrelia burgdorferi radiosensitivity and Mn antioxidant content: antigenic preservation and pathobiology by Andrés F. Londoño, Ajay Sharma, Jared Sealy, Vipin S. Rana, Shelby D. Foor, Vera Y. Matrosova, Elena K. Gaidamakova, Robert P. Volpe, Michael J. Daly, Brian M. Hoffman, Utpal Pal, J. Stephen Dumler

“…We then explored the influence of the Mn2+-decapeptide-phosphate antioxidant complex MDP, known to shield proteins during irradiation, and showed that treatment with MDP preserves B. burgdorferi’s epitopes at 5 kGy irradiation, which crucially prevents cell proliferation. …”
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A broadly neutralizing antibody against the SARS-CoV-2 Omicron sub-variants BA.1, BA.2, BA.2.12.1, BA.4, and BA.5 by Zhe Chen, Leilei Feng, Lei Wang, Li Zhang, Binyang Zheng, Hua Fu, Fengdi Li, Ligai Liu, Qi Lv, Ran Deng, YanLi Xu, Yongfeng Hu, Jianhua Zheng, Chuan Qin, Linlin Bao, Xiangxi Wang, Qi Jin

“…Structural analysis using cryo-electron microscopy (cryo-EM) revealed that CR9 binds to an epitope formed by RBD residues, providing a molecular understanding of its neutralization mechanism. …”
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A single residue switch mediates the broad neutralization of Rotaviruses by Yang Huang, Feibo Song, Yuanjun Zeng, Hui Sun, Roufang Sheng, Xuechun Wang, Liqin Liu, Guoxing Luo, Yanan Jiang, Yaling Chen, Mengxuan Zhang, Shiyin Zhang, Ying Gu, Hai Yu, Shaowei Li, Tingdong Li, Qingbing Zheng, Shengxiang Ge, Jun Zhang, Ningshao Xia

“…A combination of time-resolved cryo-electron microscopy (cryo-EM) and in situ cryo-electron tomography (cryo-ET) analysis reveals a counterintuitive dynamic process of virus inactivation, in which 7H13 asymmetrically binds to a conserved epitope in the capsid-proximal aspect of VP4, triggers a conformational switch in a critical residue—F418—thereby disrupts the meta-stable conformation of VP4 essential for normal viral infection. …”
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Tumor cell-based liquid biopsy using high-throughput microfluidic enrichment of entire leukapheresis product by Avanish Mishra, Shih-Bo Huang, Taronish Dubash, Risa Burr, Jon F. Edd, Ben S. Wittner, Quinn E. Cunneely, Victor R. Putaturo, Akansha Deshpande, Ezgi Antmen, Kaustav A. Gopinathan, Keisuke Otani, Yoshiyuki Miyazawa, Ji Eun Kwak, Sara Y. Guay, Justin Kelly, John Walsh, Linda T. Nieman, Isabella Galler, PuiYee Chan, Michael S. Lawrence, Ryan J. Sullivan, Aditya Bardia, Douglas S. Micalizzi, Lecia V. Sequist, Richard J. Lee, Joseph W. Franses, David T. Ting, Patricia A. R. Brunker, Shyamala Maheswaran, David T. Miyamoto, Daniel A. Haber, Mehmet Toner

“…Abstract Circulating Tumor Cells (CTCs) in blood encompass DNA, RNA, and protein biomarkers, but clinical utility is limited by their rarity. To enable tumor epitope-agnostic interrogation of large blood volumes, we developed a high-throughput microfluidic device, depleting hematopoietic cells through high-flow channels and force-amplifying magnetic lenses. …”
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Dynamics of stochastic mutation to immunodominance by Yu Wu, Xiaopeng Zhao, Mingjun Zhang

“…Although a virus contains several epitopes that can be recognized by cytotoxic T lymphocytes (CTL), the immune responses against different epitopes are not uniform. …”
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Characterization of physicochemical and immunogenic properties of allergenic proteins altered by food processing: a review by Enning Zhou, Qiangqiang Li, Dan Zhu, Gang Chen, Liming Wu

“…Food processing helps to reduce allergenicity by aggregating or denaturing proteins, which masks, modif ies, or destroys antigenic epitopes, whereas, it cannot eliminate allergenicity completely, and sometimes even improves allergenicity by exposing new epitopes. …”
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Design of a Multiepitope Pan-Proteomic mRNA Vaccine Construct Against African Swine Fever Virus: A Reverse Vaccinology Approach by Ella Mae Joy S. Sira, Lauren Emily Fajardo, Edward C. Banico, Nyzar Mabeth O. Odchimar, Fredmoore L. Orosco

“…Based on 100 ASFV proteomes, six epitopes that induce cytotoxic T-cell responses, five epitopes that induce helper T-cell responses, and four epitopes that induce antibody production were predicted. …”
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Are Ancient Durum Wheats Less Toxic to Celiac Patients? A Study of α-Gliadin from Graziella Ra and Kamut by M. Stella Colomba, Armando Gregorini

“…Several aminoacid substitutions or deletions were detected in p31–49, DQ2-Glia-α1 and DQ2-Glia-α2 epitopes, nevertheless, ELISA constantly showed antibody-antigen positive reactions which led us to suggest that mAbs binding was not apparently affected by polymorphisms. …”
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Screening of Diabetes-Associated Autoantigens and Serum Antibody Profiles Using a Phage Display System by Jun Lin, Zhenyu Wang, Hongtao Wang, Yuping Li, Yao Liu, Yige He, Qian Liu, Zichuan Chen, Yuan Ji

“…Conclusion: Human pancreatic and concurrent bacterial-derived protein antigens and their epitopes were identified in this research by the phage display system, which is crucial for distinguishing different types of diabetes.…”
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Potential Application of Tregitopes as Immunomodulating Agents in Multiple Sclerosis by Wassim Elyaman, Samia J. Khoury, David W. Scott, Anne S. De Groot

“…This paper highlights the potential uses of T regulatory cell epitopes (Tregitopes), natural Treg epitopes found to be contained in human immunoglobulins, as immunomodulating agents in MS. …”
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Serological cross-reactivity between Crimean-Congo haemorrhagic fever virus and Nairobi sheep disease virus glycoprotein C by Emmanuel A. Maze, George Booth, Georgina Limon, Sandra Belij-Rammerstorfer, Simona R. Tchakarova, Tsviatko Alexandrov, Clare Browning, Ginette Wilsden, Anna B. Ludi, Bryan Charleston, Teresa Lambe, Teresa Lambe

“…Based on an analysis of known epitopes in NP targeted by antibodies in sheep serum, we propose that NP is less cross-reactive because dominant epitopes are highly dissimilar between CCHFV and NSDV.DiscussionGc exhibited a strong cross-reaction while the NP was weakly cross-reactive due to dominant epitopes being highly dissimilar between CCHFV and NSDV. …”
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Leveraging mRNA technology for antigen based immuno-oncology therapies by Wei Zheng, Michele Ceccarelli, Charalampos S Floudas, Siranush Sarkizova

“…Tumor neoantigens are de novo epitopes derived from somatic mutations, avoiding T-cell central tolerance of self-epitopes and inducing immune responses to tumors. …”
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Immunopeptidomics for autoimmunity: unlocking the chamber of immune secrets by Sanya Arshad, Benjamin Cameron, Alok V. Joglekar

“…Abstract T cells mediate pathogenesis of several autoimmune disorders by recognizing self-epitopes presented on Major Histocompatibility Complex (MHC) or Human Leukocyte Antigen (HLA) complex. …”
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Anti-HER2 biparatopic antibody KJ015 has near-native structure, functional balanced high affinity, and synergistic efficacy with anti-PD-1 treatment in vivo by Zheng Wang, Yu Liu, Yunxia Xu, Lin Lu, Zhen Zhu, Baojie Lv, Xin Fang, Yao Tang, Jinhua Wang, Yu Cheng, Ying Hu, Junwen Lou, Peican Wu, Chendan Liu, Yanjun Liu, Xin Zeng, Qing Xu

“…To fully utilize the potential of bpAbs for anti-HER2 drug development, it is crucial to consider formats that offer stability and high-yield production, along with a functional balance between the two epitopes. In this study, we rationally designed a bpAb, KJ015, that shares a common light chain with two Fab arms and exhibits functionally balanced high affinity for two HER2 non-overlapping epitopes. …”
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Identification and analysis of immunological activity of two isoforms of tropomyosin in Alectryonella plicatula by Shiqiang Yang, Yexin Chen, Fei Huan, Xinrong He, Xiao Yun, Hong Liu, Guixia Chen, Guangming Liu

“…Structural analysis showed that TM-β had more α-helix and higher surface hydrophobicity than TM-α. Sequences and epitopes alignment with shellfish TMs revealed that amino acids of TM-β were more frequently recognized as IgE epitopes in other shellfish TMs than TM-α. …”
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DDO-adjuvanted influenza A virus nucleoprotein mRNA vaccine induces robust humoral and cellular type 1 immune responses and protects mice from challenge by Victoria Gnazzo, Hanaa Saleh, Ítalo A. Castro, Adrianus C. M. Boon, Amelia K. Pinto, James D. Brien, Carolina B. López

“…These results highlight the potential of DDO268 as an effective mRNA vaccine adjuvant and show that an IAV NP mRNA/DDO268 vaccine is a promising approach for generating protective immunity against conserved internal IAV epitopes.IMPORTANCEVaccines that generate neutralizing antibodies and cytotoxic CD8+ T-cells targeting conserved epitopes are ideal for effective protection against viruses. mRNA vaccines combined with the right adjuvant offer a promising solution to this challenge. …”
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