Upper Oligocene to Pleistocene planktonic foraminifera stratigraphy at North Atlantic DSDP Site 407, Reykjanes Ridge: diversity trends and biozonation using modern Neogene taxonomi... by T. M. Weitkamp, T. M. Weitkamp, M. J. Razmjooei, M. J. Razmjooei, P. N. Pearson, H. K. Coxall, H. K. Coxall

“…Initially investigated in 1979 by Poore, this study presents a taxonomic reassessment of upper Oligocene to Pleistocene planktonic foraminifera at Site 407, including species range documentation, assemblage analysis, biostratigraphic zonation, and age modelling based on planktonic foraminifera, calcareous nannofossils, and scanning electron microscopy. This study employs modern taxonomic perspectives that integrate morphological and stratophenetic frameworks for fossil species with genetic data for taxa having living representatives. …”
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A broadly neutralizing antibody against the SARS-CoV-2 Omicron sub-variants BA.1, BA.2, BA.2.12.1, BA.4, and BA.5 by Zhe Chen, Leilei Feng, Lei Wang, Li Zhang, Binyang Zheng, Hua Fu, Fengdi Li, Ligai Liu, Qi Lv, Ran Deng, YanLi Xu, Yongfeng Hu, Jianhua Zheng, Chuan Qin, Linlin Bao, Xiangxi Wang, Qi Jin

“…Structural analysis using cryo-electron microscopy (cryo-EM) revealed that CR9 binds to an epitope formed by RBD residues, providing a molecular understanding of its neutralization mechanism. …”
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A single residue switch mediates the broad neutralization of Rotaviruses by Yang Huang, Feibo Song, Yuanjun Zeng, Hui Sun, Roufang Sheng, Xuechun Wang, Liqin Liu, Guoxing Luo, Yanan Jiang, Yaling Chen, Mengxuan Zhang, Shiyin Zhang, Ying Gu, Hai Yu, Shaowei Li, Tingdong Li, Qingbing Zheng, Shengxiang Ge, Jun Zhang, Ningshao Xia

“…A combination of time-resolved cryo-electron microscopy (cryo-EM) and in situ cryo-electron tomography (cryo-ET) analysis reveals a counterintuitive dynamic process of virus inactivation, in which 7H13 asymmetrically binds to a conserved epitope in the capsid-proximal aspect of VP4, triggers a conformational switch in a critical residue—F418—thereby disrupts the meta-stable conformation of VP4 essential for normal viral infection. …”
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Single-cell atlas reveals multi-faced responses of losartan on tubular mitochondria in diabetic kidney disease by Zhen Zhu, Guangxin Luan, Song Wu, Yiyi Song, Shuang Shen, Kaiyue Wu, Shengnan Qian, Weiping Jia, Jun Yin, Tao Ren, Jianping Ye, Li Wei

“…Preliminary validation through in vitro and in vivo experiments, including observation of changes in mitochondrial morphology and dynamics using probes such as Mitotracker Red, and evaluation of the effect of losartan on key events of mitochondrial homeostasis perturbation using electron microscopy, laser confocal microscopy, immunofluorescence, and Western blotting. …”
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Electroacupuncture attenuates ferroptosis by promoting Nrf2 nuclear translocation and activating Nrf2/SLC7A11/GPX4 pathway in ischemic stroke by Xi-chen Yang, Ya-ju Jin, Rong Ning, Qiu-yue Mao, Peng-yue Zhang, Li Zhou, Cheng-cai Zhang, Yi-chen Peng, Na Chen

“…The mitochondria were observed by transmission electron microscopy (TEM). Results Electroacupuncture improved neurological deficits in rats model of MCAO/R, decreased the brain infarct volume, alleviated neuronal damage, inhibited the Fe2+, ROS, and MDA accumulation, increased SOD levels, increased the expression of GPX4, SLC7A11 and FTH1, and rescued injured mitochondria. …”
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Interpreting Variants of Uncertain Significance in PCD: Abnormal Splicing Caused by a Missense Variant of DNAAF3 by Haixia Zheng, Chongsheng Cheng, Miao He, Wangji Zhou, Yixuan Li, Jinrong Dai, Ting Zhang, Kai‐Feng Xu, Xue Zhang, Xinlun Tian, Yaping Liu

“…Minigene assays were performed to evaluate the pathogenicity of variants. Transmission electron microscopy (TEM) and high‐speed video analysis (HSVA) were conducted to analyze the function of cilia in respiratory epithelial cells. …”
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A novel high-throughput screen identifies phenazine-1-carboxylic acid as an inhibitor of African swine fever virus replication in primary porcine alveolar macrophages by Jing Lan, Rui Luo, Di Liu, Changxing Qi, Xin Song, Zhanhao Lu, Ruojia Huang, Yuying Yang, Yuan Sun, Yonghui Zhang, Tao Wang, Hua-Ji Qiu

“…The growth kinetics, hemadsorption, and transmission electron microscopy analysis of rASFV-Gluc/EGFP in primary porcine alveolar macrophages (PAMs) revealed that rASFV-Gluc/EGFP exhibits similar biological characteristics to ASFV-WT. …”
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LncRNA LUCAT1 offers protection against human coronary artery endothelial cellular oxidative stress injury through modulating hsa-miR-6776-5p/LRRC25 axis and activating autophagy f... by Yanjun Wang, Xin Zhang, Mengmeng Ren, Shu He, Hengjie Bie, Mengyang Duan, Zhiyuan Chen, Qiaowei Jia, Boyu Chi, Xiongkang Gan, Chengcheng Li, Yahong Fu, Hanxiao Zhou, Sheng Zhang, Qian Zhang, Fenghui An, Xiumei Chen, Enzhi Jia

“…Methods (1) Autophagy-related proteins including LC3, p62, Beclin1, ATG5, and ATG7 were immunohistochemical stained in coronary specimens; (2) The levels and function of autophagy in the HCAEC oxidative stress model were evaluated using western blot (WB), transmission electron microscopy (TEM), and mRFP-GFP-LC3 adenovirus transfection experiments; (3) The competing endogenous RNA (ceRNA) network of lncRNA LUCAT1/hsa-miR-6776-5p/LRRC25 axis was constructed and validated; (4) The expression levels of above autophagy-related RNAs in peripheral blood mononuclear cells (PBMCs) were verified by qPCR, and their diagnostic performance was subsequently analyzed using receiver operating characteristic (ROC) analysis. …”
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Hexane extract from Lindera communis roots: wound healing properties and membrane-disruptive activities against methicillin-resistant Staphylococcus aureus by Miaomiao Wang, Miaomiao Wang, Miaomiao Wang, Xian Hu, Xian Hu, Xian Hu, Liya Liu, Liya Liu, Liya Liu, Liya Liu, Yi Zhong, Yi Zhong, Yi Zhong, Wanlin Li, Wanlin Li, Wanlin Li, Qing Zhang, Qing Zhang, Qing Zhang, Congli Xu, Chunlin Long, Chunlin Long, Chunlin Long, Chunlin Long, Chunlin Long

“…To explore the antibacterial activities of L. communis plants, we investigated the chemicals, antibacterial activities and wound healing and of the n-hexane fraction of L. communis roots (LCH).MethodsOur study included detecting phytochemical constituents, determining minimum inhibitory concentration (MIC) for different extract fractions, analyzing growth curves, assessing membrane integrity, monitoring potential changes in the membrane using scanning electron microscopy, and evaluating wound healing in rat excisional wounds.ResultsBased on our findings, humulene-type sesquiterpenes, guaiane-type sesquiterpenes, and lauric acid were identified from the LCH, responsible for antibacterial and wound healing activities. …”
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Characteristics of the smallest brucellaphage with strong lytic ability by Hongbaiyu Liu, Hongbaiyu Liu, Youhong Zhong, Zhihong Zhang, Kehong Xu, Kehong Xu, Chunpeng Mao, Chunpeng Mao, Qiuju Yang, Lihua Yang, Binbin Yu, Ying Long, Ying Long, Xinyu Qin, Xinyu Qin, Liyuan Shi, Sheng Chang, Yuanying Shen, Peng Wang

“…In this study, a novel lytic brucellaphage designated Y17 was isolated from sheep fecal samples collected in Ludian County, Yunnan Province, China. Transmission electron microscopy revealed that Y17 was composed of an icosahedral head (48.1 ± 2 nm) and a short tail (10.8 ± 1 nm), making it the smallest brucellaphage described so far. …”
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Analysis of stomatal characteristics of maize hybrids and their parental inbred lines during critical reproductive periods by Changyu Zhang, Changyu Zhang, Yu Jin, Yu Jin, Jinglu Wang, Jinglu Wang, Ying Zhang, Ying Zhang, Yanxin Zhao, Xianju Lu, Xianju Lu, Wei Song, Xinyu Guo, Xinyu Guo

“…This study employed Jingnongke 728 and its parental inbred to capture stomatal images from various leaf positions and abaxial surfaces during key reproductive stages using rapid scanning electron microscopy. We uesd a target detection and image segmentation approach based on YOLOv5s and Unet to efficiently obtain 11 phenotypic traits encompassing stomatal count, shape, and distribution. …”
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Preparation of Composite Films Based on Methyl Cellulose/Polyvinyl Alcohol-loaded Nanosilver (AgNPs@PVA/MC) and Its Effect on Storage Quality of Ougan by Shuaishuai DAI, Weijie WU, Xiangjun FANG, Hangjun CHEN, Jun HUANG, Ruiling LIU, Huizhi CHEN, Ben NIU, Haiyan GAO

“…Among them, the composite film with 0.1% AgNPs content had the best comprehensive performance, its tensile strength reached 12.45 MPa, elongation at break was 6.47%, water vapor permeability was 21.03×10−6 g·mm/(m2·s·Pa), and oxygen permeability was 2.31×10−6 cm3/(cm2·s·Pa). Scanning electron microscopy (SEM) showed that composite film with 0.1% AgNPs content was regular, compact and evenly distributed in cross section. …”
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A key technology for synergistic backfilling of coal-based solid waste and high-salinity wastewater by Shuning DONG, Shujiang YU, Xingling DONG, Buqin ZHANG, Xiaoming GUO, Xiaodong WANG, Kai WANG, Shibin ZHU, Boqiang WU, Lei LIU

“…The mechanical properties, microstructures, and heavy metal leaching characteristics of the solidified filling paste were analyzed using the uniaxial compressive strength (UCS) test, scanning electron microscopy (SEM), and inductively coupled plasma-mass spectrometry (ICP-MS). …”
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Brucella suis ΔmapB outer membrane vesicles as an acellular vaccine against systemic and mucosal B. suis infection by Florencia Muñoz González, Florencia Muñoz González, Magali G. Bialer, Maria L. Cerutti, Silvia M. Estein, Lila Y. Ramis, Pablo C. Baldi, Pablo C. Baldi, Ángeles Zorreguieta, Ángeles Zorreguieta, Mariana C. Ferrero, Mariana C. Ferrero

“…In this study, we characterize the outer membrane vesicles (OMVs) of B. suis 1330 (wt) and those of B. suis ΔmapB (ΔmapB) mutant strain and evaluate their vaccine potential in mice.MethodsOMVs were isolated using the ultracentrifugation method and characterized through electron microscopy, Dynamic Light Scattering, SDS-PAGE and proteomics. …”
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Graphene oxide and silver effects on the arc erosion properties of Al2O3–Cu/30Cr composite by Qiujie Liu, Meng Zhou, Baohong Tian, Yi Zhang, Qimeng Zhang, Jiacan Zhang, Zhiyu Han, Chunhe Chu, Ke Jing, Xu Li, Huiwen Guo, Alex A. Volinsky

“…Furthermore, the transmission electron microscopy (TEM) and energy dispersive spectrometer (EDS) results show that Cr3C2 compounds effectively pin the Cr–Cu interface. …”
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Galactin-8 DNA methylation mediates macrophage autophagy through the MAPK/mTOR pathway to alleviate atherosclerosis by Bing Xia, Yan-lin Lu, Jin Peng, Jing-wei Liang, Fang-qin Li, Jiu-yang Ding, Chang-wu Wan, Cui-yun Le, Jia-lin Dai, Jie-Wang, Bing Guo, Jiang Huang

“…Detection of autophagosomes in macrophages by transmission electron microscopy was also performed. The foam cell model was induced with human monocytes (THP-1) and co-cultured with foam cells using siRNAs targeting GAL8, DO05, and UO126. …”
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Lycium barbarum glycopeptide ameliorates aging phenotypes and enhances cardiac metabolism by activating the PINK1/Parkin-mediated mitophagy pathway in D-galactose-induced mice by Tianchan Peng, Jian Xiang, Yun Tian, Xiaogen Tang, Lina Wang, Lijuan Gao, Oscar Junhong Luo, Li’an Huang, Guobing Chen

“…The geroprotective effects of LbGp were assessed by behavioral tests, cardiac echocardiography, pathohistological and transcriptomic analyses. Transmission electron microscopy was used to observe the ultrastructure of mitochondria. …”
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响应面优化氯化胆碱-丙三醇体系提取芝麻蛋白工艺条件 "Response surface optimization of the extraction process conditions for sesame protein using choline chloride-glycerol system"... by 曹培换1，张晨霞1，刘燕2，马宇翔1，刘华敏1，郑永战1，3 "CAO Peihuan1，ZHANG Chenxia1，LIU Yan2，MA Yuxiang1， LIU Huamin1，ZHENG Yongzhan1,3"

“…Fourier transform infrared spectroscopy and scanning electron microscopy were used to characterize the structure of sesame protein. …”
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Antiedemic Effect of the Myosin Light Chain Kinase Inhibitor PIK7 in the Rat Model of Myocardial Ischemia Reperfusion Injury by Dmitry L. Sonin, Mikhail S. Medved, Asker Y. Khapchaev, Maria V. Sidorova, Marina E. Palkeeva, Olga A. Kazakova, Garry V. Papayan, Daniil A. Mochalov, Sarkis M. Minasyan, Ilya E. Anufriev, Daria V. Mukhametdinova, Natalia M. Paramonova, Ksenia M. Balabanova, Anastasia S. Lopatina, Ilia V. Aleksandrov, Natalya Yu. Semenova, Anna A. Kordyukova, Kirill V. Zaichenko, Vladimir P. Shirinsky, Michael M. Galagudza

“…The early no-reflow detected for the first time after 30 min ischemia in this model of myocardial infarction reduces the area accessible for PIK7. Electron microscopy has shown membrane-bound blebs of endotheliocytes, which partially or completely obturate the capillary lumen, and few capillaries with signs of intercellular gap formation in samples obtained from the center of the early no-reflow zone in control and PIK7-injected rats. …”
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Enhancement of physicochemical and techno-functional properties of soy protein isolate amyloid fibrils by moderate ultrasonic pretreatment by Yunqi Ni, Tianyi Yan, Kai Fu, Congyi Xu, Liang Zhang, Donghong Liu, Wenjun Wang

“…Morphological examination by transmission electron microscopy exhibited a higher maturation state, showing more rigid and straight fibrils structure in SPF10 compared to SPF0. …”
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