Huoxiang Zhengqi dropping pills alleviate exertional heat stroke–induced multiple organ injury through sustaining intestinal homeostasis via regulating MAPK/NF-κB pathway and gut m... by Lei Li, Lei Li, Lei Li, Jun Ma, Jun Ma, Zeshi Li, Zeshi Li, Juelin Chen, Jiawei Zhou, Yawei Wang, Yankun Pei, Yitong Gong, Jianyao You, Yangyang Cao, Man Wang, Jikuai Chen, Wenjun Chang, Weiyi Ma, Hanyu Zhu, Chuhan Xiang, Shuogui Xu, Qing Song, Qing Song

“…This study showed that HXZQD alleviated EHS-induced intestinal damage by preserving barrier proteins (ZO-1, Occludin, and Ecadherin) and maintaining intestinal barrier integrity. Transmission electron microscopy confirmed the preservation of tight junction structures. …”
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Technological dental sealants: in vitro evaluation of material properties and antibiofilm potential by Witalo Pereira de Jesus, Luiza de Almeida Queiroz Ferreira, Washington Henrique Themoteo da Silva, Fernanda Vieira Belém, Ana Paula Turrioni, Cláudia Silami de Magalhães, Mila Fernandes Moreira Madeira, Ivana Márcia Alves Diniz, Marco Aurelio Benini Paschoal

“…Abstract Background Due to the launch of new self-etching and self-adhesive sealing materials combined with the release of bioactive ions in the dental market, this in vitro study aimed to compare physical values of surface roughness (RS), vickers microhardness (VM), compositional energy dispersive spectroscopy (EDS), scanning electron microscopy (SEM), microbiological tests (inhibition halo-HI and surface microbial adhesion-MAS) and mechanical microshear bond strength (MBS). …”
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Green biosynthetic silver nanoparticles from Ageratum conyzoides as multifunctional hemostatic agents: Combining hemostasis, antibacterial, and anti-inflammatory properties for eff... by Yang Li, Yinfeng Tan, Huange Zhao, Shiting Chen, Azadeh Nilghaz, Rong Cao, Songlin Zhou

“…Subsequent experiments including scanning electron microscopy, flow cytometry, western blotting, and network pharmacology analysis have revealed that AC-AgNPs can cause platelet activation and aggregation, stimulated kallikrein-kinin system, shorten activated partial thromboplastin and prothrombin time, and increase fibrinogen content. …”
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Therapeutic effect of dihydroartemisinin on Alzheimer’s disease model mice with senile macular degeneration by Gao Han, Gong Xuewu, Zhang Meng, Wang Yuejing, Wang Yuchun, Zhang Keshuang, Yao Hongbo

“…DHA and donepezil could improve the damage of cerebral cortex structure and reduce the deposition of extracellular amyloid β-protein in AD mice. Transmission electron microscopy showed that DHA and donepezil could reduce mitochondrial vacuolation and synaptic edema. …”
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Structural basis of FpGalNase and its combination with FpGalNAcDeAc for efficient A-to-O blood group conversion by Meiling Zhou, Kaishan Luo, Chao Su, Yan Sun, Zuyan Huang, Shuo Ma, Xun Gao, Jiwei Wang, Chen Zhang, Pengcheng Han, Guoqiu Wu

“…Additionally, we engineered two fusion proteins, FpGalNAcDeAc-FpGalNase and FpGalNase-FpGalNAcDeAc, which showed a 28-fold increase in catalytic efficiency compared to the enzyme mixture. Using cryo-electron microscopy, we resolved the full-length structure of FpGalNase, identifying critical active site residues involved in its catalytic mechanism. …”
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Enhanced UV-B photoprotection activity of carotenoids from the novel Arthrobacter sp. strain LAPM80 isolated from King George Island, Antarctica by Beatriz Vivian Paredes Contreras, Alane Beatriz Vermelho, Livia Casanova, Claudia de Alencar Santos Lage, Caren Leite Spindola Vilela, Veronica da Silva Cardoso, Luis William Pacheco Arge, Janine Simas Cardoso-Rurr, Sulamita Santos Correa, Felipe Raposo Passos De Mansoldo, Maria Cristina Pinheiro Pereira Reis-Mansur, Eikon Alves da Silva, Júnia Schultz, Alexandre Soares Rosado

“…Chemical characterization of the carotenoid extract identified major components as C50 carotenoids, probably decaprenoxanthin and/or sarcinaxanthin. Scanning electron microscopy revealed minimal surface changes in bacteria during carotenoid-rich phase after UV-B irradiation exposure.These findings highlight the likely ability of LAPM80 strain's C50 carotenoids to improve UV-B iiradiation resistance, indicating their potential for developing natural photoprotective compounds for the dermo-cosmetic industry.…”
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Acaricidal and insecticidal activities of entomopathogenic nematodes combined with rosemary essential oil and bacterium-synthesized silver nanoparticles against camel tick, Hyalomm... by Bander Albogami, Hadeer Darwish, Akram Alghamdi, Ahmed BahaaEldin Darwish, Wafa Mohammed Al-Otaibi, Mohamed A. Osman, Zamzam M. Al Dhafar, Abeer Mousa Alkhaibari, Abadi M. Mashlawi, Fadi Baakdah, Ahmed Noureldeen

“…Visual observation showed a color change from yellow to dark brown for AgNPs biosynthesized from P. mirabilis; the transmission electron microscopy (TEM) image and ultraviolet–visible (UV-Vis) spectrum showed well-dispersed particles with a diameter of 5–45 nm; and the greatest surface plasmon peaked at 320 nm. …”
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Integrated removal of chromium, lead, and cadmium using nano-zero-valent iron-supported biochar: Mechanistic insights and eco-toxicity assessment by Yuzhen Wei, Jialu Yu, Fasih Ullah Haider, Qinhu Zhang, Run Chu, Cai Liqun

“…An in-depth characterization of the underlying adsorption mechanism was performed by using kinetic and isotherms models such as Pseudo-first-order, Pseudo-second-order, Langmuir and Freundlich, X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM) analysis. Each HM exhibited a distinct adsorption mechanism. …”
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Oncolytic influenza virus enhances killing effect of gemcitabine against pancreatic cancer cells by MENG Zihe, MENG Zihe, XU Yongru, DENG Zhaoda

“…The virus was then comprehensively characterized through chicken red blood cell hemagglutination assay， transmission electron microscopy， and viral replication assay. CCK-8 assay was utilized to determine the impact of OvFlu-GM-CSF viruses ［multiplicities of infection （MOI）： 0， 0.1， 1.0， 3.0］ on the survival rate of pancreatic cancer cell lines （Panc02， PANC-1， SW1990， BxPC-3） and normal pancreatic ductal epithelial cells （HPDE6-C7） after treatment for 24， 48 or 72 h. …”
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Amphotericin B Encapsulation in Polymeric Nanoparticles: Toxicity Insights via Cells and Zebrafish Embryo Testing by Magno Maciel-Magalhães, Renata Jurema Medeiros, Nayara Cecília do Couto Guedes, Thais Morais de Brito, Gabriele Fátima de Souza, Beatriz Rodrigues Canabarro, Fausto Klabund Ferraris, Fábio Coelho Amendoeira, Helvécio Vinicius Antunes Rocha, Beatriz Ferreira de Carvalho Patricio, Isabella Fernandes Delgado

“…<b>Methods:</b> In this study, we produced and characterized AmB loaded and non-loaded PNPs by nanoprecipitation, dynamic light scattering, transmission electron microscopy, atomic force microscopy and spectroscopy. …”
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Ginsenoside Rg1 alleviates chronic testicular damage caused by cranial irradiation through the SCF/PI3K/Akt/mTOR pathway in mice by Qiuhua Zhou, Yiquan Ou, Xiangsheng Tian, Yujun Ning, Yuwei Mao, Weichao Zhao, Dingxin Long

“…Results: Histological staining, transmission electron microscopy, sperm analysis, and immunofluorescence demonstrated that Ginsenoside Rg1 ameliorated structural and functional damage to the testicles, enhanced sperm count (IR: 20.70 ​± ​1.62 vs. …”
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Atmospheric corrosion of carbon and galvanized steel under high rainfall conditions by Ariel Arroyo Heredia, Hector David Agudelo Arias, Maycol F. Mena, Elcy Serna Copete, F.A. Vásquez, Nerly Liliana Mosquera, Edison Banguero Palacios, Reiner Palomino Lemus, J.A. Calderón, Samir Córdoba Machado

“…The structural-micro characterization of corrosion products was evaluated via X-ray diffraction (XRD), Raman spectroscopy and scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy SEM-EDS. …”
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Development of a Cationic Polymeric Micellar Structure with Endosomal Escape Capability Enables Enhanced Intramuscular Transfection of mRNA-LNPs by Siyuan Deng, Han Shao, Hongtao Shang, Lingjin Pang, Xiaomeng Chen, Jingyi Cao, Yi Wang, Zhao Zhao

“…The cPMs were then formulated using the synthesized polymer by the dispersion–diffusion method and characterized by dynamic light scattering (DLS) and cryo-transmission electron microscopy (CryoTEM). The membrane-destabilization activity of the cPMs was evaluated by a hemolysis assay. …”
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Analytical methods for nanomaterials investigation in food and food-related materials by Daniele Passeri, Pierfrancesco Atanasio, Anacleto Proietti, Chiara Mancini, Flavio Cognigni, Giancarlo La Penna, Luca Buccini, Marco Rossi, Anna Chiara Turco, Marilena D'Amato, Angela Sorbo, Simone Dinarelli, Giulia D'Ettorre, Federica Aureli

“…., X-ray (X-ray microscopy and X-ray diffraction), scanning microscopies (electron microscopy and atomic force microscopy), optical spectroscopies (infrared spectroscopy and Raman spectroscopy), and mass spectrometry, discussing their benefits and drawbacks. …”
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Biosynthesis and activity of Zn-MnO nanocomposite in vitro with molecular docking studies against multidrug resistance bacteria and inflammatory activators by Samy Selim, Tarek M. Abdelghany, Mohammed S. Almuhayawi, Mohammed K. Nagshabandi, Muyassar K. Tarabulsi, Mohammed Yagoub Mohammed Elamir, Asmaa A. Alharbi, Soad K. Al Jaouni

“…Herein, the synthesized Zn-MnO nanocomposites were confirmed by UV-spectrophotometry with a top peak (370 nm). Transmission electron microscopy confirmed irregular particles with a spherical-like shape ranging from 25.13 to 36.21 nm. …”
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In vitro evaluation of hypochlorous acid-silver nanoparticle waterline disinfectant for dental unit waterline disinfection by Tingting Yin, Qiaowen Li, Huan Sun, Jin Zheng, Yuanyuan Wang, Yi Luo, Li Wang

“…Results The growth of biofilm on the waterlines was observed using scanning electron microscopy (SEM) and laser confocal microscopy (LCM). …”
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IQUB mutation induces radial spoke 1 deficiency causing asthenozoospermia with normal sperm morphology in humans and mice by Tingwenyi Hu, Xiangrong Tang, Tiechao Ruan, Shunhua Long, Guicen Liu, Jing Ma, Xueqi Li, Ruoxuan Zhang, Guoning Huang, Ying Shen, Tingting Lin

“…Computer-aided sperm analysis, Papanicolaou staining, and electron microscopy were applied to evaluate the motility, morphology, and ultrastructure of spermatozoa, respectively. …”
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Absence of MCJ/DnaJC15 promotes brown adipose tissue thermogenesis by Beatriz Cicuéndez, Alfonso Mora, Juan Antonio López, Andrea Curtabbi, Javier Pérez-García, Begoña Porteiro, Daniel Jimenez-Blasco, Pedro Latorre-Muro, Paula Vo, Madison Jerome, Beatriz Gómez-Santos, Rafael Romero-Becerra, Magdalena Leiva, Elena Rodríguez, Marta León, Luis Leiva-Vega, Noemi Gómez-Lado, Jorge L. Torres, Lourdes Hernández-Cosido, Pablo Aguiar, Miguel Marcos, Martin Jastroch, Andreas Daiber, Patricia Aspichueta, Juan Pedro Bolaños, Jessica B. Spinelli, Pere Puigserver, José Antonio Enriquez, Jesús Vázquez, Cintia Folgueira, Guadalupe Sabio

“…MCJKO mice, even without UCP1, a fundamental thermogenic protein, exhibit elevated BAT thermogenesis. Electron microscopy unveils changes in mitochondrial morphology resembling BAT activation. …”
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Impact of extraction methods on the properties of Carica papaya pseudostem fibers from Cameroon used as reinforcement in biocomposites by Jean Aimé Mono, Sandrine Emvoutou Ndongo, Odette Thérèse Adegono Assiene, Armel Mewoli, Rachelle Appolince Nguefack Assona, Richard Hervé Bitete, Giresse Ulrich Defo Tatchum, Claude Takoumbe

“…Fourier transform infrared spectroscopy (FTIR) of Carica papaya fibers extracted by water retting and those extracted with NaOH indicates that the cellulose, hemicellulose, and lignin functional groups are present in the fibers and are dissolved considerably as the percentage of NaOH increases. Scanning electron microscopy (SEM) in the longitudinal plane gives a visual representation of the rough and irregular surfaces without the presence of impurities on the chemically extracted fibers compared to that extracted with water. …”
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The immune checkpoint molecule B7-H4 regulates β-cell mass and insulin secretion by modulating cholesterol metabolism through Stat5 signalling by Fangzhen Xia, Ziteng Zhang, Zhen Qian, Xiaoyu Fang, Junxue Wang, Yan Wang, Guoting Sun, Yuefeng Yu, Ninjian Wang, Junke Zhen, Yan Liu, Yingli Lu

“…A Luminex assay was used to quantify cytokine changes in B7-H4 cKO mice. Electron microscopy imaging was used to observe insulin secretory vesicles in pancreatic β cells. …”
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