Recombinase-based amplification coupled with lateral flow chromatography for the specific and sensitive detection and identification of Leishmania major in cutaneous leishmaniasis... by Insaf Bel Hadj Ali, Yusr Saadi-Ben Aoun, Imen Khammeri, Hejer Souguir, Emna Harigua-Souiai, Hamed Chouaieb, Ahmed S. Chakroun, Meryem Lemrani, Aicha Kallel, Kalthoum Kallel, Nabil Haddad, Oussaima El Dbouni, Rhea N. Coler, Rhea N. Coler, Rhea N. Coler, Steven G. Reed, Akila Fathallah-Mili, Akila Fathallah-Mili, Ikram Guizani

“…Here, we aimed for a simple and rapid molecular diagnostic test to detect and identify L. major, a predominant CL causal species, which could be prone to become a control tool at the point of care, in endemic areas, using isothermal recombinase DNA amplification (recombinase polymerase amplification, RPA, or recombinase aided amplification, RAA) coupled to detection by the lateral flow (LF) chromatography on a PCRD cassette.MethodsTo develop an L. major species-specific RPA-LF assay, computational analysis of 70 Leishmania DNA targets, identified through bibliography and database searches, selected five targets. …”
Get full text

A systematic review and meta-analysis of the impact of transcranial direct current stimulation on cognitive function in older adults with cognitive impairments: the influence of do... by Thatchaya Prathum, Thanwarat Chantanachai, Oranich Vimolratana, Chotica Laksanaphuk, Irin Apiworajirawit, Benchaporn Aneksan, Kanthika Latthirun, Cheng-Ta Yang, Wanalee Klomjai

“…AD was diagnosed based on the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV), or the National Institute of Neurological and Communicative Disorders and Stroke – Alzheimer’ Disease and Related Disorders Association (NINCDS-ADRDA) criteria. …”
Get full text

Epidemiology of community-acquired pneumonia among hospitalised children in Indonesia: a multicentre, prospective study by Rina Triasih, Yan Mardian, Dewi Lokida, Helmia Farida, Herman Kosasih, Adhella Menur Naysilla, Arif Budiman, Chakrawati Hayuningsih, Moh Syarofil Anam, Dwi Wastoro, Mujahidah Mujahidah, Setya Dipayana, Amalia Setyati, Abu Tholib Aman, Nurhayati Lukman, Muhammad Karyana, Ahnika Kline, Aaron Neal, Chuen-Yen Lau, Clifford Lane

“…Objective To identify aetiologies of childhood community-acquired pneumonia (CAP) based on a comprehensive diagnostic approach.Design ‘Partnerships for Enhanced Engagement in Research-Pneumonia in Paediatrics (PEER-PePPeS)’ study was an observational prospective cohort study conducted from July 2017 to September 2019.Setting Government referral teaching hospitals and satellite sites in three cities in Indonesia: Semarang, Yogyakarta and Tangerang.Participants Hospitalised children aged 2–59 months who met the criteria for pneumonia were eligible. …”
Get full text

Multiple intracerebral hematomas during SEEG recording and intradural hemorrhage after spinal tap: A case report prompting more research on collagen IV gene mutation and oral nicot... by Markus Leitinger, Serena Broggi, Mathias Spendel, Gudrun Kalss, Ivan Petrović, Herbert Krainz, Fabio Rossini, Julia Höfler, Andreea Toma, Giorgi Kuchukhidze, Matthias Mauritz, Kai‐Nicolas Poppert, Bernardo Crespo‐Pimentel, Pilar Bosque‐Varela, Anna Pleyers, Patricia Ganger, Dieter Kotzot, Davor Lessel, Christoph J. Griessenauer, Eugen Trinka

“…Within a few days after insertion, the patient developed multiple ICHs in the electrode trajectories and an intradural hemorrhage after a diagnostic spinal tap. We performed the investigation of the clotting system and whole‐exome sequencing (WES). …”
Get full text

Disease activity in patients with idiopathic inflammatory myopathy according to time since diagnosis and positivity to antisynthetase autoantibodies: data from the Myo-Spain regist... by Tatiana Cobo-Ibáñez, Ivan Castellví, Ana Pros, Marta Domínguez-Álvaro, Laura Nuño-Nuño, Julia Martínez-Barrio, Vega Jovaní, Fredeswinda Romero-Bueno, Esther Ruiz-Lucea, Eva Tomero, Ernesto Trallero-Araguás, Javier Narváez, Jordi Camins-Fàbregas, Alberto Ruiz-Román, Jesús Loarce-Martos, Susana Holgado-Pérez, V Miguel Flores-Rodríguez, Francisca Sivera, Carolina Merino-Argumanez, Antonio Juan-Mas, Irene Altabás-González, María Martín-López, Joaquín María Belzunegui-Otano, Carmen Carrasco-Cubero, Mercedes Freire-González, Iñigo Rúa-Figueroa, Nuria Lozano-Rivas, Julio David Suarez-Cuba, Olga Martínez, Rafaela Ortega-Castro, Patricia Alcocer, Alejandro Gómez-Gómez, Olga Sánchez-Pernaute, José Luis Tandaipan, Irene Carrión-Barberà, Chamaida Plasencia-Rodríguez, Oihane Ibarguengoitia-Barrena, Paola Vidal-Montal, Vera Ortiz-Santamaria, Noemi Garrido-Puñal, Anne Riveros, Esmeralda Delgado-Frías, Juan Miguel López-Gómez, Carmen Barbadillo, José María Pego-Reigosa, Beatriz E. Joven-Ibáñez, Jesús Alejandro Valero-Jaimes, Elena Naveda, Ana Isabel Turrión-Nieves, Daniel Seoane-Mato, Francisco Javier Prado-Galbarro, M Ángeles Puche-Larrubia

“…Results A total of 554 patients with different diagnostic subgroups of IIM were included (136 incident and 418 prevalent cases), with 215 ASA-positive patients (58 incident and 157 prevalent cases). …”
Get full text

Ovarian Reserve: A Critical Indicator of Female Reproductive Health by Julia Ufnal, Anna Wolff, Maria Morawska, Dominika Lewandowska, Dominika Rosińska-Lewandoska, Marcelina Szewczyk, Klaudia Kożuchowska, Dawid Pilarz, Kinga Jarosz, Szymon Gruszka

“…It also explores emerging potential interventions as well as innovative assessment tools that could enhance diagnostic accuracy. Materials and Methods: This narrative review was conducted using PubMed and Scholar database with following search terms: “ovarian reserve”, “diminished ovarian reserve”, “ovarian aging”, "ovarian insufficiency". …”
Get full text

Worldwide patterns and trends in ovarian cancer incidence by histological subtype: a population-based analysis from 1988 to 2017Research in context by Yi-Fan Wei, Li Ning, Yi-Lin Xu, Jing Ma, Dong-Run Li, Zan-Fei Feng, Fang-Hua Liu, Yi-Zi Li, He-Li Xu, Peng Li, Yong-Pei Yu, Dong-Hui Huang, Xiao-Ying Li, Song Gao, Chun-Qing Lin, Ting-Ting Gong, Qi-Jun Wu, Jing-He Lang

“…Temporal changes in diagnostic criteria and risk factors might influence the incidence and distribution of OC and its subtypes. …”
Get full text

Early diagnosis and the effect of treatment compliance on the prognosis and progression of glaucomatous optic neuropathy by S.V. Diordiychuk, A.V. Kuroyedov, N.E. Fomin, O.V. Gapon’ko, V.V. Gorodnichiy, I.V. Kondrakova, N.M. Sol’nov

“…The specificity of this case report is a long-term observation period at one hospital and the use of standardized diagnostic tools. Moreover, dynamic monitoring was performed using the same devices to assess structural and functional alterations of the retina and optic nerve. …”
Get full text

Artificial intelligence-assisted platform performs high detection ability of hepatocellular carcinoma in CT images: an external clinical validation study by Rongxue Shan, Chenhao Pei, Qianrui Fan, Junchuan Liu, Dawei Wang, Shifeng Yang, Ximing Wang

“…The artificial intelligence (AI)-assisted platform’s performance was rigorously assessed by comparing the segmentation outcomes with standard diagnostic criteria. The average dice score of the AI-assisted platform is 0.8819, which showed a high detection performance for HCC. …”
Get full text

Concordance of Prenatal Ultrasonography and Magnetic Resonance Imaging in the Classification of Corpus Callosum Dysplasia by Hui Peng, Xi Chen, Xuejin Zou, Yan Song, Chunlan Cheng, Jianli Zhang

“…The classification results of MRI were used as the diagnostic criteria. The findings detected on ultrasound (US) were compared to those detected on MRI. and the receiver operating characteristic (ROC) curve was used to analyze the sensitivity and specificity of prenatal ultrasound in diagnosing corpus callosum anomalies. …”
Get full text

Correlation between Quantitative 13C-Urea Breath Test and Helicobacter pylori Treatment Success in a Population-Based Cohort by Doron Boltin, Zohar Levi, Tsachi Tsadok Perets, Hemda Schmilovitz-Weiss, Rachel Gingold-Belfer, Ram Dickman, Iris Dotan

“…The 13C-urea breath test result (C13-UBT) utilizes H. pylori urease activity and is a highly accurate diagnostic assay. We aimed to determine whether the magnitude of C13-UBT result is related to treatment success. …”
Get full text

Development and validation of a nomogram model for prolonged length of stay in spinal fusion patients: a retrospective analysis by Linghong Wu, Xiaozhong Peng, Yao Lu, Cuiping Fu, Liujun She, Guangwei Zhu, Xianglong Zhuo, Wei Hu, Xiangtao Xie

“…The risk factors for prolonged LOS comprised blood transfusion, operation type, use of tranexamic acid (TXA), diabetes, electrolyte disturbance, body mass index (BMI), surgical procedure performed, the number of preoperative diagnoses and operative time. The diagnostic performance of the nomogram model was satisfactory, with AUC values of 0.784 and 0.795 for the internal and external validation sets, respectively. …”
Get full text

Short-acting beta agonist, antibiotics, oral corticosteroid and association with mortality and cardiopulmonary events in patients with COPD: a retrospective cohort study in Alberta... by Mohit Bhutani, Irvin Mayers, Arsh Randhawa, Manisha Talukdar, Suzanne McMullen, Phongsack Manivong, Aaron Gelfand

“…Objective The purpose of the study was to examine the association between short-acting beta agonist (SABA), antibiotic and oral corticosteroid (OCS) use and mortality and cardiopulmonary outcomes in chronic obstructive pulmonary disease (COPD).Design Retrospective cohort study using administrative health data from 1 April 2011 to 31 March 2020.Setting Alberta, Canada.Participants Patients ≥35 years old with COPD were identified using diagnostic codes.Primary and secondary outcome measures Patient characteristics included age, sex, geographical zone and comorbidities (as defined by the Charlson Comorbidity Index). …”
Get full text

Identification and Analysis of Key Immune- and Inflammation-Related Genes in Idiopathic Pulmonary Fibrosis by Tan Y, Qian B, Ma Q, Xiang K, Wang S

“…Three genes (RNASE3, S100A12, S100A8) were obtained by crossing two machine algorithms (Boruta and LASSO),which had good diagnostic performance with AUC values. These key genes were all enriched in the same pathways, such as GOCC_azurophil_granule, IL-12 signalling and production in macrophages is the pathway with the strongest role for key genes. …”
Get full text

Subcutaneous sarilumab for the treatment of hospitalized patients with moderate to severe COVID19 disease: A pragmatic, embedded randomized clinical trial. by Westyn Branch-Elliman, Ryan Ferguson, Gheorghe Doros, Patricia Woods, Sarah Leatherman, Judith Strymish, Rupak Datta, Rekha Goswami, Matthew D Jankowich, Nishant R Shah, Thomas H Taylor, Sarah T Page, Sara J Schiller, Colleen Shannon, Cynthia Hau, Maura Flynn, Erika Holmberg, Karen Visnaw, Rupali Dhond, Mary Brophy, Paul A Monach

“…<h4>Participants</h4>Hospitalized patients with clinical criteria for moderate to severe COVID-19 but not requiring mechanical ventilation, and a diagnostic test positive for SARS-CoV-2.<h4>Interventions</h4>Sarilumab, 200 or 400 mg subcutaneous injection. …”
Get full text

Vimentin as a contributing factor in SARS-CoV-2-induced orchitis on postmortem testicular autopsy of COVID-19 cases: A case-control study by Soheila Akaberi-Nasrabadi, Azam Sabbaghi, Behzad M. Toosi, Parsa Ghorbanifaraz, Gholam-Reza Mahmoudiasl, Abbas Aliaghaei, Hajarsadat Faghihi Hosseinabadi, Shahrokh Paktinat, Mohammad-Amin Abdollahifar

Get full text

Biodesulfurization of high-sulfur oil from the Karazhanbas field of Kazakhstan with deep eutectic solvents by A.O. Akimbek, G.A. Jamalova, A.K. Yernazarova, G.K. Kaiyrmanova, B.K. Yelikbayev, M.C. Pagano, A.G. Zazybin, Kh. S. Rafikova

Get full text

Immuno-PET Imaging of Siglec-15 Using the Zirconium-89-Labeled Therapeutic Antibody, NC318 by Elaine M. Jagoda, Falguni Basuli, Colleen Olkowski, Ido Weiss, Tim E. Phelps, Karen Wong, Anita Thien Ton, Kelly C. Lane, Steve Adler, Donna Butcher, Elijah F. Edmondson, Sol Langermann, Peter L. Choyke

“…In these preclinical studies, the therapeutic mAb, NC318 (antihuman/murine Siglec-15 mAb), was labeled with zirconium-89 and evaluated in human Siglec-15 expressing cancer cells and mouse xenografts for potential use as a clinical diagnostic imaging agent. Methods. Desferrioxamine-conjugated NC318 was radiolabeled with zirconium-89 to synthesize [89Zr]Zr-DFO-NC318. …”
Get full text

Potato mosaic viruses which infect plants of tuber-bearing Solanum spp. growing in the VIR field gene bank by E. V. Rogozina, N. V. Mironenko, N. A. Chalaya, Yu. Matsushita, H. Yanagisawa

“…We examined clonal plants of 137 genotypes representing 31 species of the section Petota of the genus Solanum L. A diagnostic was carried out using ELISA, RT-PCR and indicator plants. …”
Get full text

Enhanced preoperative prediction of breast lesion pathology, prognostic biomarkers, and molecular subtypes using multiple models diffusion-weighted MR imaging by Litong He, Feng Li, Yanjin Qin, Yuling Li, Qilan Hu, Zhiqiang Liu, Yunfei Zhang, Tao Ai

“…All five diffusion models proved valuable for breast cancer diagnosis, with the CTRW model exhibiting the highest diagnostic performance, although the difference was not statistically significant. …”
Get full text