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First Autologous Cell Therapy of Cerebral Palsy Caused by Hypoxic-Ischemic Brain Damage in a Child after Cardiac Arrest—Individual Treatment with Cord Blood
Published 2013-01-01“…Nine weeks later, he received autologous cord blood (91.7 mL, cryopreserved, 5.75×10e8 mononuclear cells) intravenously. …”
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162
Mucosal leishmaniasis is associated with the Leishmania RNA virus and inappropriate cutaneous leishmaniasis treatment.
Published 2025-01-01“…Clinical data were recorded from the patient's medical records. Cryopreserved biopsies were used to detect LRV1 and identify Leishmania species.…”
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163
Exploring the Most Promising Stem Cell Therapy in Liver Failure: A Systematic Review
Published 2019-01-01“…Intravenous (IV) route was equal to invasive route. Fresh or cryopreserved, and autologous or allogeneic MSCs were equally beneficial; and giving too many cells via intraportal or the hepatic artery might be counterproductive.…”
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164
Examination of common culture medium for human hepatocytes and engineered heart tissue: Towards an evaluation of cardiotoxicity associated with hepatic drug metabolism in vitro.
Published 2024-01-01“…Therefore, we aimed to establish a system for evaluating cardiotoxicity via hepatic metabolism by co-culturing cryopreserved human hepatocytes (cryoheps) and human iPS cell-derived engineered heart tissues (hiPSC-EHTs) using a stirrer-based microphysiological system. …”
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165
Effect of a single rectal fecal microbiota transplantation on clinical severity and fecal microbial communities in dogs with chronic inflammatory enteropathy
Published 2025-01-01“…The effect of glycerol as a cryopreservative when storing FMT preparations also was assessed. …”
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166
The protective effect of trehalose and monosodium glutamate on yeast viability and antagonistic properties during freeze-drying
Published 2024-11-01“…The efficacy of protective treatments depended on the yeast isolate, the protective substance used during freeze-drying, treatment timing (prevention vs. intervention), and interactions of those factors. Cryopreserved isolate 117/10 performed better than 114/73 with the addition of either trehalose or monosodium glutamate, reducing the disease severity index from 88.3% (control) to 18.75−55.33%. …”
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167
Adipose Tissue-Derived Stromal Cells Induce a Highly Trophic Environment While Reducing Maturation of Monocyte-Derived Dendritic Cells
Published 2020-01-01“…ASCs were isolated from healthy voluntary donors, expanded using a human platelet lysate in bioreactors, and cryopreserved as per clinical use. Monocyte-derived dendritic cells were generated by isolation of monocytes and differentiation with GM-CSF and IL-4. …”
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168
Fixed timed artificial insemination in buffalo heifers with fresh and frozen-thawed semen in Argentina
Published 2023-11-01“…The fertility of frozen-thawed semen was lower than that of frozen- thawed semen, without being significant, which should motivate veterinarians and breeders to cryopreserve semen from breeding stock. It is shown how acceptable pregnancy rates are obtained independently of the semen condition, which allows visualizing the application of artificial insemination in this species to achieve the necessary improvement to satisfy the needs of the industry. …”
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169
Safeguarding and using global banana diversity: a holistic approach
Published 2020-10-01“…Abstract The CGIAR genebank International Musa Germplasm Transit Centre (ITC) currently holds 1617 banana accessions from 38 countries as an in vitro collection, backed-up by a cryopreserved collection to safeguard global Musa diversity in perpetuity. …”
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170
Single-cell RNA sequencing highlights the role of distinct natural killer subsets in sporadic amyotrophic lateral sclerosis
Published 2025-01-01“…For this purpose, peripheral blood mononuclear cells (PBMC) were isolated from 14 ALS patients and 14 cognitively unimpaired healthy individuals (HC), matched by age and gender, and cryopreserved until library preparation and scRNAseq. …”
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171
Ovarian stimulation alternatives for in vitro production of embryos in water buffaloes
Published 2023-11-01“…In vitro fertilization (IVF) was performed after 24 hours of oocyte in vitro maturation using cryopreserved semen from buffalo bulls of proven fertility. …”
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172
Exploring the effect of cold stress on the semen quality parameters of buffalo bulls
Published 2023-11-01“…., semen volume, sperm concentration, mass activity, individual motility, viability, acrosome integrity, and hypo-osmotic swelling test (HOST), were evaluated immediately after semen collection, and diluted semen was cryopreserved for further studies. Additionally, fresh and frozen sperm were subjected to flow cytometric analysis utilizing the Luminex MUSE Cell Analyzer and CytoFLEX, Beckman Coulter-Life Sciences, respectively. …”
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