Studies of biological properties of continuous suspension ВНК-21/SUSP/ARRIAH cell line by M. I. Doronin, M. N. Guseva, D. V. Mikhalishin, A. S. Sharypov, N. S. Mudrak, N. Ye. Kamalova, B. L. Manin

“…BHK-21/SUSP/ARRIAH cells recover rapidly after cryopreservation and demonstrate 95–99% viability and growth rate of 3.36–5.88 at passages 1 to 3 and 6.85–10.95 at passages 4 to 12. …”
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Germ cell quantification in human fetal and prepubertal testis tissues: a comparison of current methodologies by Emma Kearney, David Greenald, Gabriele Matilionyte, Sheila Lane, Melissa D Tharmalingam, Jill Davies, Jan-Bernd Stukenborg, Grace Forsyth, Rod T Mitchell

“…Human testicular samples included fetal tissue (exposed in vitro to cisplatin, carboplatin or control) or prepubertal tissue (fresh, cryopreserved, fresh in vitro cultured or cryopreserved in vitro cultured). …”
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The adaptation of rainbow trout to warmer water: Oxidative damage in the germinal line by Vianel Sevastei, Sonia A. Crichigno, M. Victoria Santos, Andrea Trochine, Julio C. Painefilú, Noemí Zaritzky, Víctor E. Cussac

“…Semen fatty acid composition, sperm morphology, sperm motility, and fertilization performance in samples before and after the application of cryopreservation procedures were also evaluated. The observed responses, mainly related to the sperm membrane, reinforces the idea that ROS can affect the germinal line of male rainbow trout juveniles subjected to high water temperature. …”
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Reliability of high-quantity human brain organoids for modeling microcephaly, glioma invasion and drug screening by Anand Ramani, Giovanni Pasquini, Niklas J. Gerkau, Vaibhav Jadhav, Omkar Suhas Vinchure, Nazlican Altinisik, Hannes Windoffer, Sarah Muller, Ina Rothenaigner, Sean Lin, Aruljothi Mariappan, Dhanasekaran Rathinam, Ali Mirsaidi, Olivier Goureau, Lucia Ricci-Vitiani, Quintino Giorgio D’Alessandris, Bernd Wollnik, Alysson Muotri, Limor Freifeld, Nathalie Jurisch-Yaksi, Roberto Pallini, Christine R. Rose, Volker Busskamp, Elke Gabriel, Kamyar Hadian, Jay Gopalakrishnan

“…These High Quantity brain organoids (Hi-Q brain organoids) exhibit reproducible cytoarchitecture, cell diversity, and functionality, are free from ectopically active cellular stress pathways, and allow cryopreservation and re-culturing. Patient-derived Hi-Q brain organoids recapitulate distinct forms of developmental defects: primary microcephaly due to a mutation in CDK5RAP2 and progeria-associated defects of Cockayne syndrome. …”
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Recipient synchronization with various prostaglandin f<sub>2α</sub> protocols for embryo transfer in cows by Mesut Kırbaş, Şükrü Dursun, Mehmet Köse, Bülent Bülbül, Mehmet Çolak, Hasan Mutlu

“…All cows were transferred embryos cryopreserved by ethylene glycol on d 7 (oestrus 0). …”
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Perspectives on the use of decellularized/devitalized and lyophilized human perinatal tissues for bone repair: Advantages and remaining challenges by Lauriana Solecki, Mathilde Fenelon, Halima Kerdjoudj, Roberta Di Pietro, Gianmarco Stati, Camille Gaudet, Eugenie Bertin, Jeremie Nallet, Aurélien Louvrier, Thomas Gualdi, Jessica Schiavi-Tritz, Florelle Gindraux

“…Human amniotic membrane (hAM) has been extensively used for several decades as a bioactive scaffold for regenerative medicine. In its cryopreserved form—one of the main storage formats—the presence of viable cells has often been questioned. …”
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Malunion of the Acetabulum Treated by Intra-articular Buttress by D. Saragaglia, J. Gaillot

“…We treated a protruding acetabular malunion, 20 years ago, using an intra-articular buttress, by means of an allograft corresponding to a femoral head fragment which had been cryopreserved. The radiological and clinical result with this extended follow-up is quite remarkable, which has motivated us to present this original technique.…”
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PATIENTS' CHRONOTYPE INFLUENCE IN TREATMENT EFFICIENCY OF PLACENTA CRYERXTRACT CHANGES IN MAXILLOFACIAL PHLEGMONS by K. Lokes, M. Pérez Sayáns, V. Lychman, D. Steblovskyi, I. Boiko, D. Avetikov

“…Analyzing the obtained data, it can be asserted that the most significant changes in markers of lipid peroxidation in patients with superficial phlegmons of maxillofacial localization were experienced in both experimental groups when used against the background of treatment according to standard protocols of intravenous injections of the cryopreserved placenta without and in combination with local injection of the cryopreserved placenta as part of "Levomekol" ointment, in contrast to the control group. …”
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Protocol for a patient-derived preclinical platform to model tumor-immune interactions and evaluate therapeutic efficacy by Wen-Hsin Chang, Andrew I. Chin, Ching-Hsien Chen

“…We outline steps for isolating human peripheral blood mononuclear cells (PBMCs), preparing patient-derived precision-cut tumor slices (PCTSs), cryopreserving the samples, and setting up the co-culture system. …”
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Miniaturization and characterization of patient derived hepatocellular carcinoma tumor organoid cultures for cancer drug discovery applications by David A. Close, Paul A. Johnston

“…We describe the miniaturization and growth in 96- and 384-well formats of a single non-tumor liver and two Hepatocellular carcinoma (HCC) organoids derived from cryopreserved PDTO cells and the application of high content imaging (HCI) to characterize the models and enhance drug sensitivity testing. …”
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Heart rhythm safety of the mononuclear cord blood stem cells transplantation in patients with refractory ischemic heart failure by O.Yu. Usenko, A.V. Yakushev, O.V. Lisun, M.V. Kostylev, V.F. Onishchenko

“…Results demonstrated that administration of the cell preparation «Cryopreserved human cord blood» is not associated with increased risk of life-threatening cardiac arrhythmias. …”
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Ocular Involvement and Blindness Secondary to Linear IgA Dermatosis by Cinthya Ramos-Castellón, Gabriela Ortiz-Nieva, Fernando Fresán, Leonardo Villalvazo, Yonathan Garfias, Alejandro Navas, María C. Jiménez-Martínez

“…The patient was treated with keratolimbal allogenic transplantation and cryopreserved amniotic membrane in the right eye. Regardless of the treatment he persisted with torpid evolution developing retinal and choroidal detachments. …”
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Assessing the potential of germplasm collections for the management of genetic diversity: the case of the French National Cryobank by Jacques, Alicia, Duclos, Delphine, Danchin-Burge, Coralie, Mercat, Marie-José, Tixier-Boichard, Michèle, Restoux, Gwendal

“…In response, many countries, including France, have developed gene banks in order to conserve reproductive genetic material. Cryopreserved resources can potentially be used to manage the genetic diversity of populations, but this opportunity is seldom exploited. …”
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First Autologous Cord Blood Therapy for Pediatric Ischemic Stroke and Cerebral Palsy Caused by Cephalic Molding during Birth: Individual Treatment with Mononuclear Cells by A. Jensen, E. Hamelmann

“…This patient was treated by autologous cord blood mononuclear cells (45.8 mL, cryopreserved, TNC 2.53×10e8) with a remarkable recovery. …”
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Freeze-all cycles for normal responders: Is this way forward? by Satish Sharma, Shalaka Mhatre, Ashwini Jadhav, Nikita Lad

“…Fresh embryo transfers were postponed, and all embryos were cryopreserved. Frozen–thawed embryo transfers (FET) were performed in subsequent cycles. …”
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Human Amniotic Membrane Positioning in the Surgical Treatment of Temporomandibular Joint Degenerative Disorder by Luca Guarda-Nardini, Diletta Trojan, Giulia Montagner, Elisa Cogliati, Matteo Bendini, Daniele Manfredini

“…In the present case report, the authors describe the application of a cryopreserved HAM patch within the joint space as a disc-replacing film during major surgeries for discectomy and arthroplasty. …”
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Mesenchymal Stem Cells in Restoration of Fertility at Experimental Pelvic Inflammatory Disease by Nataliia Volkova, Mariia Yukhta, Anatoliy Goltsev

“…Based on this, we investigated the effect of intravenous administration of cryopreserved mesenchymal stem cells (cMSCs) of bone marrow on experimental chronic inflammation of the ovaries. …”
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Articular Cartilage Repair Using Marrow Stimulation Augmented with a Viable Chondral Allograft: 9-Month Postoperative Histological Evaluation by James K. Hoffman, Sandra Geraghty, Nicole M. Protzman

“…However, marrow stimulation typically results in fibrocartilage repair tissue rather than healthy hyaline cartilage, which, over time, predisposes the repair to failure. Recently, a cryopreserved viable chondral allograft was developed to augment marrow stimulation. …”
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Rationale and Design of the First Double-Blind, Placebo-Controlled Trial with Allogeneic Adipose Tissue-Derived Stromal Cell Therapy in Patients with Ischemic Heart Failure: A Phas... by Jens Kastrup, Morten Schou, Ida Gustafsson, Olav W. Nielsen, Rasmus Møgelvang, Klaus F. Kofoed, Charlotte Kragelund, Jens Dahlgaard Hove, Andreas Fabricius-Bjerre, Merete Heitman, Mandana Haack-Sørensen, Lisbeth Drozd Lund, Ellen Mønsted Johansen, Abbas Ali Qayyum, Anders Bruun Mathiasen, Annette Ekblond

“…It is produced without animal products, in closed bioreactor systems and cryopreserved as an off-the-shelf product ready to use. …”
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Pilot Study: Human Adipose Tissue Allograft for Fat Pad Defects in Patients With Preulcerative Lesions by Matthew J. Regulski, DPM, Molly C. Saunders, BS, Sharron E. McCulloch, MS, Alla Danilkovitch, PhD

“…To address this, a human cryopreserved adipose tissue (hCAT) allograft has been developed to repair adipose tissue defects. …”
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