Microbiological tests of fresh bull semen collected at breeding establishment by M. A. Sushkova, I. Ya. Stroganova, S. А. Schislenko

“…Since antimicrobials contained in the diluent used during semen product cryopreservation do not allow an objective assessment of semen contamination, a microbiological testing of fresh undiluted bull semen was carried out at the AO «Krasnoyarskagroplem» breeding establishment to identify the contamination source. …”
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Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates by Ligiane de Oliveira Leme, Mauricio Machaim Franco, Otávio Augusto de Faria, Alexandre Rodrigues Caetano, Juliana Gonçalves de Souza, Luciano de Rezende Carvalheira, Emivaldo de Siqueira Filho, Margot Alves Nunes Dode

“…Subsequently, we selected SOF25 for comparison with SOF100 in a commercial laboratory setting, evaluating embryo production, response to cryopreservation, gestation rate, and offspring birth. …”
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Personalising glioblastoma medicine: explant organoid applications, challenges and future perspectives by Niclas Skarne, Rochelle C. J. D’Souza, Helen M. Palethorpe, Kylah A. Bradbrook, Guillermo A. Gomez, Bryan W. Day

“…Recent technological developments have permitted the maintenance, expansion and cryopreservation of GBM explant organoid (GBO) tissue. …”
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Cell Surface Proteins for Enrichment and In Vitro Characterization of Human Pluripotent Stem Cell-Derived Myogenic Progenitors by Sin-Ruow Tey, Madison Mueller, Megan Reilly, Colton Switalski, Samantha Robertson, Mariko Sakanaka-Yokoyama, Masatoshi Suzuki

“…We also demonstrated that cells in unsorted, CD271+, and CD15– fractions responded differently to cryopreservation and prolonged culture expansion. Lastly, we showed that CD271 expression is essential for terminal differentiation of human PSC-derived myogenic progenitors. …”
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REDERIVATION BY EMBRYO TRANSFER IN STRAINS OF LABORATORY MICE AND RATS by S. Ya. Amstislavsky, T. N. Igonina, I. N. Rozhkova, E. Yu. Brusentsev, A. A. Rogovaya, D. S. Ragaeva, V. A. Naprimerov, E. A. Litvinova, I. Z. Plyusnina, A. L. Markel

“…The optimized model of rederivation offered here involves a combination of such embryotechnological methods as freezing/cryopreservation of embryos, their washing through the number of fresh volumes of sterile media, growing in vitro for 48 hours, and subsequent transfer into either one or both uterine horns of recipient females. …”
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Correlation between Post-Thaw Spermatozoa Quality of the Endangered Javan Banteng with OPN Gene Expression by Vincentia Trisna Yoelinda, Raden Iis Arifiantini, Dedy Duryadi Solihin, Muhammad Agil, Dedi Rahmat Setiadi, Tulus Maulana, Bambang Purwantara, Yohana Tri Hastuti, Jansen Manansang, Dondin Sajuthi

“…The development of semen cryopreservation may assist on one side of the conservation plan. …”
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In situ-formed cryomicroneedles for intradermal cell delivery by Mengjia Zheng, Tianli Hu, Yating Yang, Xuan Qie, Huaxin Yang, Yuyue Zhang, Qizheng Zhang, Ken-Tye Yong, Wei Liu, Chenjie Xu

“…Specifically, S-cryoMNs are made by dipping a porous MN scaffold in the cell suspension before cryopreservation. The porous scaffold can be transported at room temperature, and researchers can load any cells with the optimized cryogenic medium. …”
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Fertility preservation counselling and practice among women with lymphomas: a retrospective study by Mariola Hernández Martínez, César Lizán Tudela, Blanca Carreras Gamón, Laura Gregori Navarro

“…Of the 13 patients who were informed about fertility preservation, 2 patients refused this intervention, and fertility preservation was contraindicated for 3 patients, leading to a total of eight women who underwent fertility preservation strategies: six underwent ovarian cortex cryopreservation, and two underwent oocyte vitrification. …”
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The Sarcoplasmic/Endoplasmic reticulum Ca2+-ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation by Ferran Garriga, Jesús Martínez-Hernández, Ainhoa Parra-Balaguer, Marc Llavanera, Marc Yeste

“…Abstract Liquid storage is the primary preservation method in the swine breeding industry because of its advantages over cryopreservation. Calcium (Ca2+), a key regulator of cell physiology, plays a crucial role during liquid preservation. …”
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Applying reproductive technologies and genome resource banking to laboratory animals by S. Ya. Amstislavsky, E. Yu. Brusentsev, T. O. Abramova, D. S. Ragaeva, I. N. Rozhkova, T. N. Igonina, E. A. Kizilova, V. A. Naprimerov, N. Yu. Feoktistoiva

“…Some other laboratory animal species have been cryopreserved as well. Embryos of two hamster species (Djungarian and Campbell’s) in the genus Phodopus were cryopreserved and the viability of thawed embryos was proved by their successful development in vitro and in vivo (by transfer to a recipient). …”
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The Effect of Storage Time of Fresh Human Amniotic Membrane on the Levels of Growth Factors Epidermal Growth Factor, Transforming Growth Factor Beta, and Basic Fibroblast Growth Fa... by Ida Bagus Nyoman Ananta Awatara, Yuanita Safitri Dianti, Sitti Rizaliyana, Vijayendran Swaminathan

“…Introduction: The fresh amnion membrane is a popular option for wound healing. After cryopreservation, fibroblasts and epithelial cells in the amnion membrane remain viable, but their viability decreases when the membrane is freeze dried. …”
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Effect of cooling and freezing on the kinematic parameters of ram spermatozoa sexed by modified protocol with TLR7/8 ligand R848 by Yotov Stanimir, Kistanova Elena, Atanasov Anatoli, Ivanova Boyana, Zaimova Darina

“…The present study investigated the effect of sex-sorting by TLR7/8 ligand R848, cooling and cryopreservation on the motility and kinematic characteristics of ram spermatozoa for the first time. …”
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Immunomodulatory Effects of 1,25-Dihydroxyvitamin D3 on Dendritic Cells Promote Induction of T Cell Hyporesponsiveness to Myelin-Derived Antigens by Wai-Ping Lee, Barbara Willekens, Patrick Cras, Herman Goossens, Eva Martínez-Cáceres, Zwi N. Berneman, Nathalie Cools

“…Furthermore, the feasibility to cryopreserve highly potent tolDC will, ultimately, contribute to the large-scale production and the widely applicable use of tolDC.…”
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Intratesticular Angiolipoma: A Rare Case of Adipose Tissue Presence in the Testis by V. Kalyvas, C. Gkekas, D. Papadopoulos, A. Malioris, S. Milias, M. Papathanasiou, N. Kalinderis, K. Moysidis, K. Hatzimouratidis

“…After sonographic and MRI investigation, the patient was referred for semen analysis and cryopreservation. Subsequently, the patient underwent testicular biopsy (frozen section biopsy) and right partial orchiectomy. …”
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Exploring the need for fertility education: A cross-sectional study of healthcare professionals and students’ knowledge of fertility and fertility preservation by Ana GALHARDO, Catarina SALVADO, Soraia MONIZ, Marina Cunha, Ilda MASSANO-CARDOSO

“…Participants' answers regarding factors affecting fertility showed that more than half of the participants, more than half of the students (54.6%), and the professionals (60%) overestimated the age of women’s fertility decline. Oocyte cryopreservation was the fertility preservation option they knew best. …”
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Proteomics and metabolomics analyses of mechanism underlying bovine sperm cryoinjury by Renzheng Zhang, Xiuyuan Wang, Ruili Liu, Yanfang Mei, Xiuping Miao, Jiaxu Ma, Lei Zou, Qiuyue Zhao, Xuejin Bai, Yajuan Dong

“…Abstract Background The cryoinjury of semen during cryopreservation reduces sperm motility, constraining the application of artificial insemination (AI) in bovine reproduction. …”
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Evaluation and diagnostic approach in patient with Perrault Syndrome by Rachael Christin Nathania, Steven Yulius Usman, Ekarini Aryasatiani

“…Women at risk of ovarian failure should consider donor eggs or oocyte cryopreservation. Avoiding aminoglycosides and excessive noise is crucial for managing hearing loss.…”
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Metabolic phenotype of adult mice offspring obtained from different variants of embryo transfer by M. V. Anisimova, Ya. Gong, N. S. Yudin, Yu. M. Moshkin, L. A. Gerlinskaya

“…In the management of collections of genetic lines of laboratory animals, these technologies are obligatory components of cryopreservation and rederivation. ART procedures include the development of early embryos outside the mother’s body and the high probability of incomplete synchronization of the physiological state of the surrogate mother and transplanted embryos. …”
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Controlled ovarian hyperstimulation with or without letrozole for fertility preservation in breast cancer patients: study protocol for a randomised controlled trial by Ping Li, Kun Wang, Qi Fan, Peng Sun, Yuhua Shi, Yanqiu Xie, Weifen Deng, Jiajing Kang

“…The standard options for fertility preservation are oocyte and embryo cryopreservation; both require controlled ovarian hyperstimulation (COH). …”
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In vitro study of resveratrol as an antioxidant for boar semen preservation: A systematic review by Ragil Angga Prastiya, Trilas Sardjito, Amung Logam Saputro, Sari Yanti Hayanti, Nining Haryuni, Samira Musa Sasi

“…However, boar sperm are particularly susceptible to oxidative damage during storage and cryopreservation because of their high polyunsaturated fatty acid content. …”
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