Comparative evaluation of ACetic - MEthanol high salt dissociation approach for single-cell transcriptomics of frozen human tissues by Marina Utkina, Anastasia Shcherbakova, Ruslan Deviatiiarov, Ruslan Deviatiiarov, Ruslan Deviatiiarov, Alina Ryabova, Marina Loguinova, Valentin Trofimov, Anna Kuznetsova, Mikhail Petropavlovskiy, Rustam Salimkhanov, Denis Maksimov, Eugene Albert, Alexandra Golubeva, Walaa Asaad, Lilia Urusova, Ekaterina Bondarenko, Anastasia Lapshina, Alexandra Shutova, Dmitry Beltsevich, Oleg Gusev, Oleg Gusev, Oleg Gusev, Oleg Gusev, Larisa Dzeranova, Galina Melnichenko, Ildar Minniakhmetov, Ivan Dedov, Natalya Mokrysheva, Sergey Popov

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3DCryoHolder: a new open access 3D printable system to store and transport silicon nitride membranes under cryogenic conditions for spectromicroscopy at low temperature by Antonio Dominguez-Alfaro, Carlos Sanchez-Cano

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LOW TEMPERATURE STORAGE OF ACTINOBACILLUS PLEUROPNEUMONIAE CULTURES by V. S. Rusaleyev, D. A. Vasilyev

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Insights on the role of cryoprotectants in enhancing the properties of bioinks required for cryobioprinting of biological constructs by Harshavardhan Budharaju, Dhakshinamoorthy Sundaramurthi, Swaminathan Sethuraman

Subjects: “…Cryopreservation…”
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The plant germplasm and herbarium (WIR) collections maintained at VIR as contributors to grape genetic diversity conservation, expansion and utilization by M. M. Agakhanov, L. V. Bagmet, N. G. Tikhonova, M. V. Erastenkova, E. N. Kislin, Yu. V. Ukhatova, E. K. Khlestkina

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CRYOCONSERVATION OF POTATO BREEDING CULTIVARS AT VIR by Y. V. Ukhatova, E. V. Oves, N. N. Volkova, T. A. Gavrilenko

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Flow Cytometry of the Side Population: Tips & Tricks by Irene Sales-Pardo, Ariadna Avendaño, Vanessa Martinez-Muñoz, Marta García-Escarp, Raquel Celis, Phil Whittle, Jordi Barquinero, Joan Carles Domingo, Pedro Marin, Jordi Petriz

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Investigation of the post-cryogenic regeneration ability of potato varieties under different cultivation conditions by E. S. Bespalova, Yu. V. Ukhatova, N. N. Volkova, E. V. Oves, N. A. Gaitova, T. A. Gavrilenko

“…Cryopreservation provides long-term storage of the gene pool of potato varieties in cryobanks at extremely low temperatures. …”
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A Sweet Potion to Put Embryonic Stem Cells to Sleep by Kaushik D. Deb

“…Here we discuss the need for a natural cryoprotectant and biopreservative with a potential to improve cryopreservation, ambient temperature storage, and shipping of hESCs and derivatives. …”
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Fertility Preservation for Cancer Patients: A Review by Tosin Ajala, Junaid Rafi, Peter Larsen-Disney, Richard Howell

“…These include ethical issues, such as valid consent and research in the area of tissue retrieval, cryopreservation, and transplantation.…”
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A simplified computational liver perfusion model, with applications to organ preservation by Daniel Emerson, Yoed Rabin, Levent Burak Kara

“…However, developing new cryopreservation protocols requires exploration of a complex design space, further complicated by the scarcity of real human livers to experiment upon. …”
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Conservation of plant biodiversity by biotechnology methods by N. V. Romadanova, S. V. Kushnarenko

“…Several methods of plant preservation are currently used in the Republic of Kazakhstan: 1) field gene banks in natural growing areas and pomological collections; 2) preservation of seeds at +4°С, –18°С, and –196°С; 3) сold storage of in vitro shoots at +4°С and +10 ± 2°С; 4) cryopreservation of plant tissues and organs at –196°C; 5) preservation of plant DNA at –80°C.Ex situ field collections of plants in Kazakhstan are maintained in botanical gardens of the Republic, arboretums, pomological gardens, nurseries, peasant and farm enterprises, and personal plots. …”
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International Heart Valve Bank Survey: A Review of Processing Practices and Activity Outcomes by Wee Ling Heng, Helmi Albrecht, Paul Chiappini, Yeong Phang Lim, Linda Manning

“…Differences in procedures included (1) type of sterile media used, (2) antibiotics combination, (3) temperature and duration used for bioburden reduction, (4) concentration of DMSO used for cryopreservation, and (5) storage duration for released allografts. …”
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Fertility Preservation in Girls by Jennia Michaeli, Michael Weintraub, Eitan Gross, Yehuda Ginosar, Vardit Ravitsky, Einat Eizenman, Eduardo Mitrani, Meital Lebovich, Neri Laufer, Stephen Kennedy, Ariel Revel

“…Pediatric hemato-oncology, reproductive endocrinology, surgery, anesthesia and bioethics perspectives are discussed and integrated to propose guidelines for offering ovarian cryopreservation to premenarcheal girls with cancer.…”
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Both Freshly Prepared and Frozen-Stored Amniotic Membrane Cells Express the Complement Inhibitor CD59 by Ágnes Füst, Éva Pállinger, Adrienn Stündl, Eszter Kovács, László Imre, Sára Tóth, János Németh

“…Therefore it is most important to study if cryopreservation of the membrane affects cell surface expression of the molecules. …”
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Fertility Preservation among Cancer Patients in Saudi Arabia: Knowledge and Attitudes of Medical Students by Karim Hamda Farhat, Mostafa Ahmed Arafa, Danny Munther Rabah, Deana Khalid Awartani, Shahad Hussein Bajunaid, Seham Majid Abdulkader, Khaled Arab Awartani, Alanoud Abdullah Albekairi, Mohamed Abdullah Al-Atawi, Mashael Marzouq AlShebly

“…Results: Students, particularly females, were more knowledgeable about different FP methods, such as Gonadotrophin releasing hormones, sperm cryopreservation, and oocyte cryopreservation. They stated that cost, lack of information, and access to FP services are the most common factors hindering the utilization of services. …”
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A successful pregnancy with severe male and female factor infertility treated with laser-assisted thinning by Subhendu Pandit, Prashant Sharma, Hrishikesh Magdum, Rachna Gulati, Manish Singh Ahuja

“…Although newer treatment modalities continue to boom, maternal age, in vitro culture conditions and cryopreservation affect the elasticity and thinning of the zona, which may affect hatching and subsequent implantation – keeping the rates of success low. …”
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Umbilical Cord Tissue-Derived Cells as Therapeutic Agents by Olga Maslova, Miroslav Novak, Peter Kruzliak

“…The necessity to adapt existing methods of cell isolation and culturing to GMP conditions is mentioned, as well as possible cryopreservation of this material. Light is shed on some future perspectives for UC-derived cells.…”
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Extinction Status, Challenges, and Conservation Approaches of South African Indigenous and Village Chickens: A Systematic Review by Sindisiwe Mbali Sithole, Khathutshelo Agree Nephawe, Takalani Judas Mpofu, Bohani Mtileni, Masindi Lottus Mphaphathi, Jabulani Nkululeko Ngcobo

“…Articles addressing origins, phenotypic and genetic diversity, the conservation of indigenous chickens, semen cryopreservation of indigenous chickens, climate change effects on indigenous chickens, and the use of extenders with indigenous chickens were included in this review. …”
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Multiple Approaches for Individualized Fertility Protective Therapy in Cancer Patients by I. Demeestere, F. Moffa, F. Peccatori, C. Poirot, E. Shalom-Paz

“…Various options, such as cryopreservation of ovarian tissue, have been developed and are currently routinely proposed in many centers. …”
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