Molecular Mechanisms of Synergistic Effect of PRIMA‐1met and Oxaliplatin in Colorectal Cancer With Different p53 Status by Xiao‐lan Li, Jianbiao Zhou, Nicole Xin‐Ning Tang, Yi Chai, Meng Zhou, Ai‐di Gao, Zhong‐kai Lu, Han Min

“…Methods Cell viability was assessed with Cell Counting Kit‐8 (CCK‐8) assay and combination index (CI) was calculated using The Chou‐Talalay method. …”
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Skullcapflavone II suppresses TGF-β-induced corneal epithelial mesenchymal transition in vitro by Meng-Xi Li, Zhen Zhang, Yue Zhang, Fan-Ru Zhao, Yu-Fan Li, Yu-Fei Dang, Yang-Yang Yue, Li Li

“…At a SCF-II (5, 10 µmol/L) dose, cell viability was analysed with a cell counting kit-8 (CCK-8) assay, and cell migration was monitored with wound healing and Transwell migration assays. …”
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Image-based analysis of quantitative morphological characteristics of wild potato tubers using the desktop application SeedСounter by K. A. Ivanova, E. G. Komyshev, M. A. Genaev, A. A. Egorova, K. A. Koloshina, N. A. Chalaya, D. A. Afonnikov, A. V. Kochetov, E. V. Rogozina, S. V. Gerasimova

“…The number of tubers per plant was counted; the following characteristics were extracted from the images of individual tubers: length, width, projected area, length to width ratio, сircularity, roundness, rugosity and solidity. …”
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Circulating Baseline CXCR3+Th2 and Th17 Cell Proportions Correlate With Trabecular Bone Loss After 48 Weeks of Biological Treatment in Early Rheumatoid Arthritis by Julia M. Scheffler, Christina Drevinge, Catharina Lindholm, Inger Gjertsson, Kristina Lend, Merete Lund Hetland, Mikkel Østergaard, Till Uhlig, Marte Schrumpf Heiberg, Espen A. Haavardsholm, Michael T. Nurmohamed, Jon Lampa, Tuulikki Sokka‐Isler, Dan Nordström, Kim Hørslev‐Petersen, Bjorn Gudbjornsson, Gerdur Gröndal, Ronald vanVollenhoven, Hans Carlsten, Mattias Lorentzon, Anna‐Karin Hultgård Ekwall, Anna Rudin, Ulrika Islander

“…Disease activity was measured using the Clinical Disease Activity Index, swollen or tender joint counts, C‐reactive protein levels, and erythrocyte sedimentation rates. …”
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Awareness and knowledge regarding female genital schistosomiasis among European healthcare workers: a cross-sectional online survey by Valentina Marchese, Aaron Remkes, Irina Kislaya, Pia Rausche, André Brito, Jana Christina Hey, Tahinamandranto Rasamoelina, Rivo Andry Rakotoarivelo, Jürgen May, Daniela Fusco

“…Practices related to FGS were described using counts and proportions for a subsample of MDs aware of FGS. …”
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Gene content of seawater microbes is a strong predictor of water chemistry across the Great Barrier Reef by Marko Terzin, Steven J. Robbins, Sara C. Bell, Kim-Anh Lê Cao, Renee K. Gruber, Pedro R. Frade, Nicole S. Webster, Yun Kit Yeoh, David G. Bourne, Patrick W. Laffy

“…., benthic cover of corals and macroalgae, fish counts/biomass) and may be applicable to other large-scale reef metagenomics datasets beyond the GBR. …”
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Inflammatory Indices and MAFLD Prevalence in Hypertensive Patients: A Large-Scale Cross-Sectional Analysis from China by Shen D, Cai X, Hu J, Song S, Zhu Q, Ma H, Zhang Y, Ma R, Zhou P, Yang W, Hong J, Zhang D, Li N

“…The diagnosis of MAFLD was established using metabolic dysfunction criteria alongside evidence of hepatic steatosis confirmed through imaging. Complete blood counts were used to calculate inflammatory indices, including the monocyte-to-lymphocyte ratio (MLR), neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), systemic inflammatory response index (SIRI), systemic immune-inflammation index (SII), and aggregate index of systemic inflammation (AISI). …”
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The Impact of Nutritional Status and Tumor-Infiltrating Lymphocytes (CD4+ and CD8+) on Chemotherapy Response in Colorectal Cancer Patients by Lukman K, Septianto R, Rudiman R, Ruchimat T, Sribudiani Y, Nugraha P, Primastari E, Budiman D

“…Regarding chemotherapy responses in patients with colorectal cancer, this study aims to investigate the association between nutritional status and infiltrating lymphocyte counts, specifically CD4+ and CD8+.Materials and Methods: This prospective observational study analyzed the impact of nutritional status using body mass index (BMI) and TILs levels (CD4+/CD8+) on chemotherapy outcomes in CRC patients treated at a tertiary hospital in West Java, Indonesia, from July 2023 to June 2024.Results: Thirty-six research subjects were included. …”
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Sex differences in intracranial plaque burden in patients with type 2 diabetes mellitus with acute ischemic cerebrovascular disease: a pilot study based on high-resolution MRI by Xuejiao Yan, Ling Li, Jie Gao, Lihui Wang, Kai Ai, Xiaoyan Lei, Min Tang, Xiaoling Zhang, Dongsheng Zhang

“…ICAS was assessed by 3T cardiovascular magnetic resonance vascular wall imaging. Plaque counts of the total, proximal, and distal intracranial arteries were used to assess plaque burden. …”
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A Digital Tool for Clinical Evidence–Driven Guideline Development by Studying Properties of Trial Eligible and Ineligible Populations: Development and Usability Study by Shahzad Mumtaz, Megan McMinn, Christian Cole, Chuang Gao, Christopher Hall, Magalie Guignard-Duff, Huayi Huang, David A McAllister, Daniel R Morales, Emily Jefferson, Bruce Guthrie

“…The disclosure control mechanism is integral to the tool, limiting tabular counts to meet local governance needs. An exemplary result for gout as an index condition is presented to demonstrate the tool’s functionality. …”
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Effect of miR-144-3p-Targeted Regulation of PTEN on Proliferation, Apoptosis, and Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells under Stretch by Shiyong Ling, Xi Luo, Bo Lv, Hua Wang, Mengzhi Xie, Kai Huang, Jingchuan Sun

“…The cell proliferation was detected by Cell Counting Kit-8 (CCK-8) colorimetric assay; the expression levels of cyclin, cyclin-dependent kinases (CDK), BCL2-associated X (BAX), B-cell lymphoma-2 (BCL-2), and other cell cycle and apoptosis related proteins were detected by western blot (WB); and the osteogenic differentiation ability of MSC cells was detected by ALP staining and alizarin red staining. …”
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Application of synbiotic with different probiotic doses to prevent vibriosis in humpback grouper by Dwi Agung Saputra, , Sukenda, , Widanarni

“…After a feeding trial period, there were observation of the bacterial counts in the fish intestine, the fish growth performance and immune response. …”
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Clinical Study on Lumbar Disc Herniation Treated by Sling and Tuina Exercise Technique by Yi DING, Chenchen GUO, LI Li

“…The recurrence rates of the three groups were counted at six months'follow-up.Results:The cure rate of the sling and tuina exercise technique group was higher than that of the other two groups(<italic>P</italic>&lt;0.05). …”
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Comparison of Clinical Characteristics and Mortality Outcome in Critical COVID-19 Patients Infected with Alpha and Omicron Variants by Cheng HI, Chang KW, Wu BC, Teo MY, Hung WS, Wu HM, Huang ACC, Lin CW, Lin TY, Lin HC, Chiu CH, Lin SM

“…The Alpha group had a higher body mass index, had a longer ICU stay, and included more patients with acute respiratory distress syndrome, and the Omicron group included more active smokers, had more comorbidities, had worse initial laboratory data (including higher white blood cell counts, prothrombin time [PT], activated partial prothrombin time, blood urine nitrogen levels, and creatine levels), and had higher in-hospital mortality rates (40.6% vs 15.2%, p = 0.004). …”
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Framework for smartphone-based grape detection and vineyard management using UAV-trained AI by Sergio Vélez, Mar Ariza-Sentís, Mario Triviño, Antonio Carlos Cob-Parro, Miquel Mila, João Valente

“…Viticulture benefits significantly from rapid grape bunch identification and counting, enhancing yield and quality. Recent technological and machine learning advancements, particularly in deep learning, have provided the tools necessary to create more efficient, automated processes that significantly reduce the time and effort required for these tasks. …”
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Effects of saffron-grape intercropping on saffron flower number and rhizosphere microbial community by Yuanyuan Tao, Guifen Zhou, Xingchang Zhang, Mengqing Feng, Liqin Li, Xiaodong Qian

“…However, the impact of intercropping saffron with other plants on saffron yields and soil microbial community diversity remains unclear. In our study, we counted the number of saffron flowers in two cropping patterns (saffron monoculture and saffron-grape intercropping), and analyzed the microbial community diversity and composition using Illumina high-throughput sequencing methods based on 16 S and ITS amplicons. …”
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High risk for life-threatening adverse events of fluoroquinolones in young adults: a large German population-based cohort study by Julia Wicherski, Jonas Peltner, Cornelia Becker, Katrin Schüssel, Gabriela Brückner, Andreas Schlotmann, Helmut Schröder, Winfried V. Kern, Britta Haenisch

“…Patients during fluoroquinolone episodes were older (59 versus 51 years) and more often female (58% versus 54%). We counted 46,502; 446,727; 19,125; and 474,411 incident endpoints. …”
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Characterizing Glycemic Control and Sleep in Adults with Long-Standing Type 1 Diabetes and Hypoglycemia Unawareness Initiating Hybrid Closed Loop Insulin Delivery by Susan Kohl Malone, Amy J. Peleckis, Laura Grunin, Gary Yu, Sooyong Jang, James Weimer, Insup Lee, Michael R. Rickels, Namni Goel

“…Shorter sleep onset latency (d=1.53; p<0.01), shorter sleep duration (d=0.79), fewer total activity counts (d=1.32), shorter average awakening length (d=0.46), and delays in sleep onset (d=1.06) and sleep midpoint (d=0.72) occurred with medium-large effect sizes from baseline to 9 months. …”
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High tumor mutational burden predicts favorable response to anti-PD-(L)1 therapy in patients with solid tumor: a real-world pan-tumor analysis by Sehhoon Park, Jaeyun Jung, You Jeong Heo

“…In contrast, exhausted T-cell and M2 macrophage counts were increased in the non-responder group.Conclusions The overall incidence of TMB status was analyzed by the TSO500 assay, and TMB-H was observed in 14.7% of the pan-cancer population. …”
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Effect of supplementing epinephrine in maturation media on the developmental competence of buffalo oocytes by Ali Husnain, Abdul Khaliq, Talha Ashraf, Abdul Rehman, Muhammad Yasir Zahoor, Muhammad Binyameen, Amjad Riaz

“…Maturation and fertilization media were prepared in-house. Blastomere was counted from the hatched blastocyst after staining with Hoechst 33342 using an inverted microscope equipped with Cellsens software. …”
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