Activation of Delta-Opioid Receptor Protects ARPE19 Cells against Oxygen-Glucose Deprivation/Reoxygenation-Induced Necroptosis and Apoptosis by Inhibiting the Release of TNF-α by Runjie Guo, Ping Chen, Tiantian Fu, Ren Zhang, Yuan Zhu, Nange Jin, Hong Xu, Yong Xia, Xuesong Tian

“…Cell viability was quantified using the Cell Counting Kit-8 (CCK-8) assay. Morphological changes were observed by inverted microscopy. …”
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Do Activity Sensors Identify Physiological, Clinical and Behavioural Changes in Laying Hens Exposed to a Vaccine Challenge? by Hyungwook Kang, Sarah Brocklehurst, Marie Haskell, Susan Jarvis, Victoria Sandilands

“…With behaviour observations from videos, the proportions of sitting, foraging and feeding behaviours (<i>p</i> = 0.044, 0.036 and 0.004, respectively), the proportion of total visit duration to the litter zone (<i>p</i> < 0.001) and perch (<i>p</i> = 0.037) with TrackLab and the proportions of visit counts of hens in the litter zone (<i>p</i> = 0.012) from video scanning changed significantly with days. …”
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Microplastics in beach sediments, seawater, and common fish in tourist destinations by M. Go, A. Ybanez, A. Ilano, F. Cababat, L. De La Calzada

“…The quantification of microplastics was conducted under a stereomicroscope.FINDINGS Moalboal exhibited higher average microplastic counts in sediments (0.12 items per gram) and seawater (0.06 items per gram) than Badian, which had 0.08 items per gram in sediments and 0.04 items per gram in seawater. …”
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Danggui-Shaoyao-San Can Ameliorate Alzheimer&rsquo;s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology by Zhang KX, Zhang JW, Jiang YH, Wang YR, Liu ZL, Ding PL, Wang XY, Cui WQ, Xu XQ, Wang YH

“…Flow cytometry and Cell Counting Kit-8 (CCK-8) reveal the effect of DSS serum on HT-22 proliferation and apoptosis.Results: A total of 57 metabolic components were screened in DSS serum. …”
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Dietary rosemary oil with/without zymogen forte improves water quality, growth hormones, immune-physiological response, stress resilience, and health status of Chelon ramada grown... by Ashraf I. G. Elhetawy, Mohammed F. El Basuini, Ahmed I. A. Mansour, Shimaa A. Shahin, Amira A. Omar, Mohamed M. Zayed, Mohamed M. Abdel-Rahim

“…Additionally, there were decreases in the feed conversion ratio (FCR) and the levels of nitrogen by-products (NH4, NH3, and NO2) and pathogenic bacterial counts in the rearing water when fish were fed diets supplemented with RO and RO + ZF. …”
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Investigation of Woody Species Structure and Regeneration Status in the Central Rift Valley, Sidama Regional State, Ethiopia by Assegid Assefa, Tamrat Bekele, Sebsebe Demissew, Tesfaye Abebe

“…In each quadrat, all woody species were identified, counted, and recorded. In each quadrat, all tree and shrub species higher than ≥2 m in height and ≥2 cm in diameter at breast height were measured by a calibrated wooden stick and by a caliper, respectively. …”
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Evaluating the Growth of Staphylococcus aureus During Slow Cooking of Beef and Turkey Formulations from 10 °C to 54.4 °C for an Extended Time by Subash Shrestha, Michelle Riemann, Vijay K. Juneja, Abhinav Mishra

“…Maximum growth of 1.1 ± 0.2 (p < 0.05), 0.9 ± 0.3 (p < 0.05), and 0.2 ± 0.1 (p > 0.05) log CFU/g was observed by the 6th h in beef with no antimicrobial, lactate diacetate, and vinegar, respectively, and 1.6 ± 0.2 (p < 0.05), 1.3 ± 0.3 (p < 0.05), and 0.5 ± 0.3 (p > 0.05) log CFU/g in the turkey formulations also by the 6th h. The counts declined thereafter (p < 0.05) in all formulations, reaching below the inoculation level by 9.3 h. …”
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Epidemiology, clinical spectrum, and outcomes of severe malaria in Eastern Uganda: a prospective study by Cate Namayanja, George Paasi, Jimmy Patrick Alunyo, Denis Amorut, Charles Benard Okalebo, William Okiror, Paul Ongodia, Grace Abongo, Rita Muhindo, Yovani A. M. Lubaale, Peter Olupot-Olupot

“…Laboratory analyses included complete blood counts, lactate, glucose, blood gases, electrolytes, metabolites, and coagulation markers. …”
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GPR137-RAB8A activation promotes ovarian cancer development via the Hedgehog pathway by Chao Tang, Lin Li, Chongying Zhu, Qiang Xu, Zihao An, Shouying Xu, Chao Lin

“…The biological effects of GPR137-RAB8A-Hedgehog(HH) were investigated using in vitro and in vivo models and methods including qRT-PCR, RNA stability assay, RNA immunoprecipitation assay, GLI-luciferase reporter assay, nucleo-cytoplasmic separation assay, membrane-cytoplasmic separation assay, western blot, co-immunoprecipitation, immunofluorescence staining, cell counting kit-8 assay, wound healing assay, matrigel invasion assay, colony formation assay, xenografts assay, in situ transplantation tumor model of ovarian cancer in nude mice, and immunohistochemistry staining. …”
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多巴胺受体4对肝癌细胞增殖转移的影响及预后 by 严俨, 张红英, 潘家浩, 王冬音, 周晓爽, 元云飞, 曾维安, 陈东泰

“…【方法】通过免疫组化以及实时定量PCR（qPCR）技术检测肝    癌以及癌旁组织 DRD4 蛋白和 mRNA 表达情况，并分析 DRD4 表达的预后意义；采用 Cell Counting Kit 8（CCK8） 和 Transwell 实验检测 PD-168077 以及 L-745870 对肝癌细胞增殖、迁移及侵袭的作用，蛋白免疫印迹（Western blot）技术检测信号通路蛋白，并使用通路抑制剂验证。…”
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Analysis of geothermal impact on metabolite compounds of heat-tolerant plant species using clustering and similarity cliff by N.B. Maulydia, K. Khairan, T.E. Tallei, F. Mohd Fauzi, R. Idroes

“…The analysis of flexophore similarity revealed 23 neighbor counts, signifying a significant abundance of fatty acids as a predominant. …”
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Fusidic Acid and Lidocaine-Loaded Electrospun Nanofibers as a Dressing for Accelerated Healing of Infected Wounds by Alsulami KA, Bakr AA, Sirwi A, Elfaky MA, Shaik RA, Alshehri BY, Aodah AH, Al Fayez N, Alshehri AA, Almughem FA, Halwani AA, Tawfik EA

“…The prepared nanofibers were tested in vivo in an S. aureus-infected wound mice model to assess their efficacy, and the results showed that the FA/LDC-PVP had a faster wound closure and the lowest bacterial counts compared to other groups.Conclusion: These findings showed the potential application of the fabricated dual drug-loaded nanofibers as a wound-healing plaster against infected acute wounds.Keywords: electrospinning, nanofibers, fusidic acid, lidocaine, wound healing, infected wound…”
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A Novel, Patient-Specific Mathematical Pathology Approach for Assessment of Surgical Volume: Application to Ductal Carcinoma in situ of The Breast by Mary E. Edgerton, Yao-Li Chuang, Paul Macklin, Wei Yang, Elaine L. Bearer, Vittorio Cristini

“…We tested this idea in a retrospective study of 17 patients by staining breast tumor specimens containing ductal carcinoma in situ for mitosis with Ki-67 and for apoptosis with cleaved caspase-3 and counting cells positive for each marker. We also determined diffusion penetration by measuring the thickness of viable rims of tumor cells within ducts. …”
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Temporal Changes in Plasmodium Falciparum Genetic Diversity and Multiplicity of Infection Across Three Areas of Varying Malaria Transmission Intensities in Uganda. by Mwesigwa, Alex, Kiwuwa, Steven M., Musinguzi, Benson, Kawalya, Hakiim, Katumba, James Davis, Baguma, Andrew, Mutuku, Irene M., Adebayo, Ismail Abiola, Nsobya, Samuel L., Byakika‐Kibwika, Pauline, Kalyango, Joan N., Karamagi, Charles, Nankabirwa, Joaniter I.

“…MOI was assessed by counting distinct genotypes. Multi-locus linkage disequilibrium (LD) and genetic differentiation were evaluated using the standardized index of association ( IAS) and Wright’s fixation index (FST), respectively. …”
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CAM/TMA-DPH as a promising alternative to SYTO9/PI for cell viability assessment in bacterial biofilms by Tinatini Tchatchiashvili, Tinatini Tchatchiashvili, Mateusz Jundzill, Mateusz Jundzill, Mike Marquet, Kamran A. Mirza, Kamran A. Mirza, Mathias W. Pletz, Mathias W. Pletz, Oliwia Makarewicz, Oliwia Makarewicz, Lara Thieme, Lara Thieme

“…Viability findings were compared with colony-forming units (CFU/mL), a standard microbial viability measure.ResultsCAM/TMA-DPH staining demonstrated strong positive correlations with CFU counts across all bacterial species (r = 0.59 - 0.91), accurately reflecting biofilm vitality. …”
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Mortality risk prediction model in AIDS patients with pneumocystis pneumonia in China by Xi Wang, Letian Liu, Wen Wang, Yang Zhang, Hui Chen, Zhangli Wang, Jianwei Li, Yue Gao, Yanqun Huang, Lijun Sun, Tong Zhang, Aixin Li

“…We found that the best prediction effect can be obtained when ALB, PO2, TBIL, LDH, CD4+ T lymphocyte counts are incorporated into the PCP risk prediction model. …”
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N1-methylnicotinamide promotes age-related cochlear damage via the overexpression of SIRT1 by Toru Miwa, Toru Miwa, Akihito Tarui, Akihito Tarui, Teppei Kouga, Yasunori Asai, Yasunori Asai, Hideaki Ogita, Hideaki Ogita, Taro Fujikawa, Nobuhiro Hakuba, Nobuhiro Hakuba

“…Cochlear histology revealed reduced hair cell and spiral ganglion cell counts, as well as decreased Na+/K+-ATPase α1 expression and endocochlear potential. …”
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Multi-omics dissection of metabolic dysregulation associated with immune recovery in people living with HIV-1 by Lin-Yu Wan, Sin Man Lam, Hui-Huang Huang, Wen-Jing Cao, Xiang-Yi Cao, Xue-Meng Li, Li-Ping Zhang, Jia-Min Gao, Chao Zhang, Xing Fan, Yan-Mei Jiao, Guanghou Shui, Fu-Sheng Wang, Jin-Wen Song

“…Notably, 3-hydroxyoctanoic acid and thymine were consistently associated with CD4 T cell counts and inflammation-related markers in PLWH, regardless of ART. …”
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<i>Vismia guianensis</i> Improves Survival of <i>Tenebrio molitor</i> and Mice During Lethal Infection with <i>Candida albicans</i> by Arthur André Castro Costa, Elizangela Pestana Motta, Aluísio Silva Oliveira, Pamela Gomes Santos, Josivan Regis Farias, Danielle Cristine Gomes Franco, Mayara Cristina Pinto Silva, Nicolle Teixeira Barbosa, Simone Batista Muniz, Luís Douglas Miranda. Silva, Lucilene Amorim Silva, Claudia Quintino Rocha, Flavia Raquel Fernandes Nascimento, Rosane Nassar Meireles Guerra

“…The extract increased the mice’s survival (60%) and life expectancy, reducing the CFU counts in the peritoneum and blood. EHVG also increased the number of blood neutrophils and peritoneal macrophages. …”
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Probiotic Bacillus pumilus LV149 enhances gut repair, modulates microbiota, and alters transcriptome in DSS-induced colitis mice by Xinyu Sun, Long Yun, Keming Xie, Renhui Liu, Xinyue Ren, Bokun Zeng, Xudong Cao, Zhi Li, Guihao Zhou, Bang Liu, Luo Peng, Lihong Yuan

“…The intervention also restored gut barrier function by upregulating mucins, goblet cell counts, and tight junction proteins (ZO-1, occludin, and claudin-1) in colonic tissue, along with reducing serum lipopolysaccharide (LPS) levels. …”
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