The DNMT1/miR-34a Axis Is Involved in the Stemness of Human Osteosarcoma Cells and Derived Stem-Like Cells by Xiao Liang, Chang Xu, Wanchun Wang, Xiang Li

“…We here revealed the higher DNMT1 activity and expression, lower miR-34a expression with high methylation of its promoter, and stronger stemness of OSLCs, as manifested by elevated sphere and colony formation capacities; CD133, CD44, ABCG2, Bmi1, Sox2, and Oct4 protein amounts in vitro; and carcinogenicity in a nude mouse xenograft model, when compared to the parental U2OS cells. 5-Azacytidine (Aza-dC) repressed DNMT1 activation and upregulated miR-34a expression by promoter demethylation and suppressed the stemness of OSLCs in a dose-dependent manner. …”
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The effect of triple CFTR modulator therapy and azithromycin on ion channels and inflammation in cystic fibrosis by Suhad Bani Melhim, Lisa E.J. Douglas, James A. Reihill, Damian G. Downey, S. Lorraine Martin

“…Subsequent analysis by ELISA showed IL-8, IL-6, CXCL1 and granulocyte–macrophage colony-stimulating factor to be significantly reduced by treatment with ETI, but not by AZ. …”
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Reusable Tourniquets as Potential Transmitters of Infection: A Microbiological Analysis by Julia Szymczyk, Monika Kurpas, Bartosz Krasiński, Katarzyna Zorena, Wioletta Mędrzycka-Dąbrowska

“…Bacterial contamination on the surfaces of the tourniquets was measured using Columbia agar blood medium and expressed as colony-forming units (CFUs) per cm². Results: Significant bacterial loads were detected on reusable tourniquets, with contamination levels varying by location and duration of use. …”
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Potential risks of bacterial plant pathogens from thawing permafrost in the Alaskan tundra by Dockyu Kim, Mincheol Kim, Sungho Woo, Sungjin Nam, Nu Ri Myeong, Eungbin Kim, Yung Mi Lee

“…Bacterial abundance, measured by colony-forming units and 16S rRNA gene copies, distinctly increased in the T and P layers after thawing. …”
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Human Suprapatellar Fat Pad-Derived Mesenchymal Stem Cells Induce Chondrogenesis and Cartilage Repair in a Model of Severe Osteoarthritis by Ignacio Muñoz-Criado, Jose Meseguer-Ripolles, Maravillas Mellado-López, Ana Alastrue-Agudo, Richard J Griffeth, Jerónimo Forteza-Vila, Ramón Cugat, Montserrat García, Victoria Moreno-Manzano

“…In addition, the suprapatellar SVF exhibited increased numbers of colony formation units and a higher population doubling in culture compared to the infrapatellar fraction. …”
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Novel Circulating Tumour Cell-Related Risk Model Indicates Prognosis and Immune Infiltration in Lung Adenocarcinoma by Lu Liang, Yueying Liu, Shiyao Jiang, Jingjing Huang, Hua He, Li Shen, Li Cong, Yiqun Jiang

“…In addition, western blot, CCK-8 and colony formation assays were used to validate the biological function of RAB26 in LUAD. …”
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The Maternal Cytokine and Chemokine Profile of Naturally Conceived Gestations Is Mainly Preserved during In Vitro Fertilization and Egg Donation Pregnancies by Alicia Martínez-Varea, Begoña Pellicer, Vicente Serra, David Hervás-Marín, Alicia Martínez-Romero, José Bellver, Alfredo Perales-Marín, Antonio Pellicer

“…The main outcome measures were, firstly, to follow up plasma levels of interleukin (IL) 1beta, IL2, IL4, IL5, IL6, IL8, IL10, IL17, interferon gamma, tumor necrosis factor-alpha (TNFα), transforming growth factor-beta (TGFβ), regulated upon activation normal T-cell expressed and secreted (RANTES), stromal cell-derived factor 1 alpha (SDF1α), and decidual granulocyte-macrophage colony-stimulating factor (GM-CSF) during the three trimesters of pregnancy during the three trimesters of pregnancy; secondly, to evaluate if the cytokine and chemokine pattern of ED pregnant women differs from that of those with autologous oocytes and, thirdly, to assess if women with preeclampsia show different cytokine and chemokine profile throughout pregnancy versus women with uneventful pregnancies. …”
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Effects of Thyme (Thymus vulgaris) Essential Oil on Bacterial Growth and Expression of Some Virulence Genes in Salmonella enterica Serovar Enteritidis by Mohammad Hassanzadeh, Sara Mirzaie, Faezeh Rahimi Pirmahalle, Ramak Yahyaraeyat, Jamshid Razmyar

“…Methods The bacterial growth rate and the expression of four virulence genes in S. enteritidis during 18–72 h of exposure to the essential oil at 25%–75% MIC were evaluated via colony counting and real‐time polymerase chain reaction (PCR), respectively. …”
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Cultivable Anaerobic Microbiota of Infected Root Canals by Takuichi Sato, Keiko Yamaki, Naoko Ishida, Kazuhiro Hashimoto, Yasuhisa Takeuchi, Megumi Shoji, Emika Sato, Junko Matsuyama, Hidetoshi Shimauchi, Nobuhiro Takahashi

“…Samples from infected root canals were collected, followed by anaerobic culture on CDC blood agar plates. After 7 days, colony forming units (CFU) were counted and isolated bacteria were identified by 16S rRNA gene sequencing. …”
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Scoulerine promotes cytotoxicity and attenuates stemness in ovarian cancer by targeting PI3K/AKT/mTOR axis by Wang Fang, Zhang Yang, Pang Rui, Shi Shaohong, Wang Ran

“…Scoulerine inhibited the colony-forming ability, migration and invasiveness of OVCAR3 cells in a dose-dependent fashion. …”
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Evaluation of Sociodemographic Factors among Diabetic Patients with Urinary Tract Infections in Kisii Referral Hospital, Kenya by Vincent M. Mageto, Oliver W. Mbuthia, Caroline J. Ngetsa, Dinah O. Moraa, Erick O. Okoyo, Scholastica G. Mathenge, Wachuka G. Njoroge

“…Classification of a positive culture for urinary tract infection was based on more than 100,000 (≥105) colony-forming units of a single bacterial species. …”
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Resistance to P. brasiliensis Experimental Infection of Inbred Mice Is Associated with an Efficient Neutrophil Mobilization and Activation by Mediators of Inflammation by Felipe Fornias Sperandio, Gisele Pesquero Fernandes, Ana Carolina Silvério Cerqueira Mendes, Giulia Maria de Alencar Castro Bani, Vera Lucia Garcia Calich, Eva Burger

“…We also assessed the fungal colony forming units and the levels of soluble inflammatory mediators (LTB4, KC, IFN-γ, GM-CSF, and IL-10) inside subcutaneous air-pouches to compare the efficiency of the PMN present at this site in relation to the two main neutrophil functions: initial lysis of the invading pathogen and modulation of the acquired immune response. …”
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Postoperative Pulmonary Hemodynamics and Systemic Inflammatory Response in Pediatric Patients Undergoing Surgery for Congenital Heart Defects by Maria Francilene S. Souza, Juliano G. Penha, Nair Y. Maeda, Filomena R. B. G. Galas, Kelly C. O. Abud, Eloisa S. Carvalho, Ana Maria Thomaz, Claudia R. P. Castro, Juliana Pereira, Antonio Augusto Lopes

“…Compared with patients with PAP/SAPIPO≤0.40 (n=22), those above this level (n=18) had increased pre- and postoperative serum levels of granulocyte colony-stimulating factor (p=0.040), interleukin-1 receptor antagonist (p=0.020), interleukin-6 (p=0.003), and interleukin-21 (p=0.047) (panel for 36 human cytokines) and increased mean platelet volume (p=0.018). …”
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Basic Developmental Characteristics of the Fall Armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), Reared under Laboratory Conditions by Dinah Marri, Samuel Adjei Mensah, Daniel Ashie Kotey, John Abraham, Maxwell Kelvin Billah, Michael Osae

“…Smith), was studied using a colony established from field-collected larvae. Eggs, neonate larvae, and newly emerged adult moths were used in experiments to investigate the basic biology of the FAW. …”
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Efficacy of probiotic Bacillus licheniformis DSM 28710 on performance and the mitigation of Clostridium perfringens-induced necrotic enteritis in broiler chickens by W. Van der Veken, V. Hautekiet, E.A. Kimminau, C. Hofacre, G.F. Mathis

“…A broiler trial was conducted, examining three treatments for 42 days under an induced NE challenge; a negative control (basal diet only); an antibiotic treated group (oxytetracycline hydrochloride (OXT), therapeutic dose of 105 mg OXT/litre in drinking water, for three days after C. perfringens challenge); and a B-Act group (500 g B-Act/tonne of feed, equalling 1.6×1012 colony forming units B. licheniformis DSM 28710/tonne of feed, supplemented from start until finish). …”
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The protective effect of trehalose and monosodium glutamate on yeast viability and antagonistic properties during freeze-drying by Joanna Krzymińska, Jolanta Kowalska

“…Yeast dehydrated after freeze-drying, protected with trehalose and monosodium glutamate, displayed the same colony count on tomato leaves as before freeze-drying. …”
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Prosaposin – A plausible biomarker in head and neck squamous cell carcinoma by Avradeep Datta, Rajandeep Kaur, Anshika Chauhan, Geeta Singh Boora, Himanshu Garg, Debajyoti Chatterjee, Jaimanti Bakshi, Sushmita Ghoshal, Arnab Pal

“…Cell proliferation, cell migration, cell invasion, and colony formation were found to be significantly reduced with the downregulation of PSAP. …”
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Peptidoglycan-Like Components in Z-100, Extracted from Mycobacterium tuberculosis Strain Aoyama B, Increase IL-12p40 via NOD2 by Takayuki Horii, Yuki Orikawa, Yuta Ohira, Runa Eta, Uiko Tominaga, Takanori Sato, Takao Tanaka

“…Mouse bone marrow-derived cells and CD14+ cells were cultured in the presence of granulocyte-macrophage colony-stimulating factor, differentiated into macrophage-like cells, and then stimulated with Z-100. …”
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Paramètres socio-spatiaux, réseaux sociaux et budget d'activité chez un groupe de mâles chimpanzés : stabilité dans le temps et effet de la réduction spatiale by Nerea Amezcua‑Valmala, Daniel Vázquez, Álvaro Ballesteros, Marcos Bella, Catarina Casanova, Fernando Colmenares

“…Across time, changes in maturity and the death of a member did not affect proximity patterns in this colony. The mentioned increase of self-directed behaviors is usually interpreted as an index of stress; however, our data, as agonism and other measures remained stable from 2007 to 2009, should be interpreted with caution. …”
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A vacuum lyophilization and bacterial tablet-based method for culture medium evaluation and potential use in probiotic products by Wen Cui, Liang Zhao, Yuwen Shi, Wei Feng, Xiao Chen, Hui Liu

“…The filter membrane as a carrier ensures uniform cell dispersion, forming a singular colony that can facilitate counting. We examined the viability and storage durability of vacuum freeze-dried bacterial tablets using a filter membrane as a carrier and utilized the results to evaluate culture medium. …”
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