MAGI2-AS3 hypermethylated in promoter region promotes migration and invasion of head and neck squamous cell carcinoma via miRNA-31-5p/AR axis by Kai Yue, Ting Zhang, Huanhuan Wang, Bo Wang, Yalin Mu, Hui Li

“…Molecular regulatory mechanism of MAGI2-AS3 in HNSCC is not yet mature.In this study, we analyzed the methylation level of MAGI2-AS3 promoter and its downstream miR-31-5p/AR axis by bioinformatics methods. qRT-PCR was used to detect the mRNA expression level of each gene, and western blot was used to detect the expression level of AR proteins in tissues and cells. CCK-8, colony formation, wound healing, and cellular invasion assays were used to detect the HNSCC cell proliferation, migration, and invasion. …”
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Inflammatory Cytokine Pattern Is Sex-Dependent in Mouse Cutaneous Melanoma Experimental Model by Mihaela Surcel, Carolina Constantin, Constantin Caruntu, Sabina Zurac, Monica Neagu

“…Cytokine/chemokine pattern was assessed using xMAP technology and the following molecules were quantified: interleukins (IL)-1-beta, IL-6, IL-10, IL-12 (p70), interferon (IFN)-gamma, granulocyte macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor (TNF)-alpha, macrophage inflammatory protein (MIP)-1alpha, monocyte chemoattractant protein (MCP-1), and keratinocyte-derived chemokine (KC). …”
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Antimicrobial activity of adipose-derived mesenchymal stromal cell secretome against methicillin-resistant Staphylococcus aureus by Fatimah Shaaban, Elie Salem Sokhn, Charbel Khalil, Fatima A. Saleh

“…Methods Using the Kirby-Bauer disk diffusion method, broth microdilution assays, and colony-forming unit (CFU) counting, the antibacterial activity of AD-MSC secretome was assessed. …”
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Oxymatrine inhibits the development of radioresistance in NSCLC cells by reversing EMT through the DcR3/AKT/GSK-3β pathway by Jianming Tang, Yu Cao, Hong Zhang, Rui Wang

“…The proliferation of A549 cells was measured using the Cell Counting Kit-8 and colony forming assays. In addition, the apoptosis of A549 cells was analyzed by flow cytometry. …”
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Specificity of personal characteristics of juvenile offenders with different levels of guilt awareness by N. V. Hresa, I. V. Zhdanova, O. G. Marchenko

“…The results of the study on the generalisation of psychological characteristics of juvenile offenders with different levels of guilt awareness during their adaptation to the conditions of the educational colony are presented. Based on the theoretical analysis of the philosophical, psychological, scientific and legal literature and the study of legislative acts, the authors characterise guilt as a psychological formation which includes cognitive (assessment of criminal law consequences of a criminal act), behavioural (desire to confess to a crime, to condemn one's actions in front of other convicts, assessment of the likelihood of committing a crime in the future) and emotional (assessment of the attitude towards the crime, its consequences, the victim and the court verdict) components. …”
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Evaluation of the effect on antimicrobial properties, color, roughness and curing time of glass ionomer cement incorporated with silver and copper nanoparticles by Laís Aragão Lima, Wanessa Fernandes Matias Regis, Fábio Ruan Louzeiro Lima, Victor Teixeira Noronha, Amauri Jardim de Paula, Fernanda Freitas Sousa, Paulo Goberlânio Barros Silva, Rafael Melo Freire, Lidiany Karla Azevedo Rodrigues

“…The biofilms were then collected and plated on Mitis-Salivarius-Bacitracin (MSB) agar and Sabouraud dextrose agar for Streptococcus mutans and Candida albicans, respectively. Colony-forming unit (CFU) counts and dry weight were measured to assess the biofilm growth. …”
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Research on vehicle scheduling for forest fires in the northern Greater Khingan Mountains by Jie Zhang, Junnan He, Shihao Ren, Pei Zhou, Jun Guo, Mingyue Song

“…Through simulation experiments, the proposed Improved Genetic Algorithm (IGA) achieved an average rescue time reduction of 8.5% compared to conventional Genetic Algorithm (GA) and 3.5% compared to Improved Artificial Bee Colony (IABC) algorithm, with an average solution time of 9.4 ms.…”
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Exploring the Orca Predation Algorithm for Economic Dispatch Optimization in Power Systems by Vivi Aida Fitria, Arif Nur Afandi, Aripriharta

“…This research shows that Orca Predation Algorithm consistently does better than other bio-inspired algorithms like Particle Swarm Optimization, Whale Optimization Algorithm, Grey Wolf Optimizer, the Bat Algorithm, Genetic Algorithm and Ant Colony Optimization in terms of minimum cost, average cost, and solution stability. …”
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Surface Modification by Combination of Dip-Pen Nanolithography and Soft Lithography for Reduction of Bacterial Adhesion by Santiago Arango-Santander, Alejandro Pelaez-Vargas, Sidónio C. Freitas, Claudia García

“…S. mutans adhesion was assessed by colony-forming units (CFUs), MTT viability assay, and scanning electron microscopy (SEM). …”
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Unit commitment in microgrid systems using artificial intelligence techniques by Barnabas I. Gwaivangmin, Ganiyu A. Bakare, Ya’U S. Haruna, Abdullahi L. Amoo

“…The study compared three techniques: particle swarm optimization (PSO), Enhanced hybrid particle swarm optimization–ant colony optimization (E-HPSO–ACO), and Enhanced hybrid particle swarm optimization–simulated annealing (E-HPSO–SA). …”
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Molecular subtype of gastric cancer based on apoptosis-related genes reveals differential immune microenvironment and intratumoral microorganisms distribution by Xuan Yu, Yongfu Shao, Haotian Dong, Jianing Yan, Xinjun Zhang, Guoliang Ye

“…Finally, knocking down key genes expression significantly promoted cell proliferation, colony formation and invasion of GC. Conclusions Our study conducted a comprehensive analysis of the molecular characteristics of ARGs in GC, revealed their association with the tumor immune microenvironment and intratumoral microorganisms. …”
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Antimicrobial Effects of Dental Luting Glass Ionomer Cements on Streptococcus mutans by Sina Klai, Markus Altenburger, Bettina Spitzmüller, Annette Anderson, Elmar Hellwig, Ali Al-Ahmad

“…Five different glass ionomer based luting cements were tested for their antimicrobial effects on Streptococcus mutans in two different experimental setups: (i) determination of colony-forming units (CFUs) in a plate-counting assay; (ii) live/dead staining (LDS) and fluorescence microscopy. …”
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Sustained-Release Fillers for Dentin Disinfection: An Ex Vivo Study by Bernhard Funk, Sharonit Sahar-Helft, David Kirmayer, Michael Friedman, Doron Steinberg

“…The obtained dentine powder was collected in test tubes containing phosphate-buffered saline, sonicated, and plated on agar plates. Colony-forming units were counted after 48 h of incubation. …”
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Anticancer effects of 7,8-dihydromethysticin in human leukemia cells are mediated via cell-cycle dysregulation, inhibition of cell migration and invasion and targeting JAK/STAT pat... by Xiao Yi, Deng Taoran, Jiang Lijun, Wang Di

“…Cell proliferation was assessed through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and effects on colony formation were examined via clonogenic assay. …”
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Enterococcus durans SL70, a Novel Exopolysaccharide Producer from Traditional Sourdough Fermentation of Einkorn (Triticum monococcum L. ssp. monococcum) by Berna Genc, Seyda Merve Karatas, Merve Tuğçe Tunç

“…Lactic acid bacteria were isolated and purified using the single colony technique on MRS and M17 media. The isolates were characterised using matrix-assisted laser desorption ionization-time of flight mass (MALDI-TOF) spectrometry. …”
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Notch Fatigue Damage Evolution Mechanism of TC21 Alloy with Multilevel Lamellar Microstructures by Xiaosong Zhou, Xiang Li, Chaowen Huang, Quan Wu, Fei Zhao

“…The findings reveal that increasing aging temperature primarily contributes to the augmentation of α colony (α_c) thickness, grain boundaries α phase (GBα) thickness, and α fine (α_fine) size alongside a reduction in α lath (α_lath) thickness and α_fine content. …”
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Village-Indigenous Chicken Bacterial Carriage after the Heavy Rains of 2018, Kenya: Indicator on Environmental Contamination with Pathogenic/Zoonotic Bacteria by Acsa Igizeneza, Lilly Caroline Bebora, Philip Njeru Nyaga, Lucy Wanjiru Njagi

“…Intestines from the slaughterhouses had different mean bacterial counts: Kangemi had the highest (1.3 × 1012 colony-forming units per ml), followed by Burma (5.6 × 1011), then Kariokor (4.7 × 1011). …”
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Quantitative Assessment of Optimal Bone Marrow Site for the Isolation of Porcine Mesenchymal Stem Cells by J. S. McDaniel, B. Antebi, M. Pilia, B. J. Hurtgen, S. Belenkiy, C. Necsoiu, L. C. Cancio, C. R. Rathbone, A. I. Batchinsky

“…Analyses of bone marrow aspirate (BMA) and concentrated bone marrow aspirate (cBMA) included bone marrow volume, platelet and nucleated cell yield, colony-forming unit fibroblast (CFU-F) number, flow cytometry, and assessment of differentiation potential. …”
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Synthesis and Spectroscopic Studies of Axially Ligated Zn(II)5,10,15,20-meso-tetra(p-chlorophenyl)porphyrin with Oxygen and Nitrogen Donors by Gauri D. Bajju, Sujata Kundan, Ashu Kapahi, Deepmala Gupta

“….,” which shows that with the increase in the concentration of the complexes, the colony diameter decreases and hence percent inhibition increases.…”
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Genetic markers for the resistance of honey bee to Varroa destructor by M. D. Kaskinova, L. R. Gaifullina, E. S. Saltykova, A. V. Poskryakov, A. G. Nikolenko

“…The establishing of gene pathways involved in resistance to Varroa will help create a methodological basis for the selection of Varroa resistant honeybee colonies.…”
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