Polymerized-Type I Collagen Downregulates Inflammation and Improves Clinical Outcomes in Patients with Symptomatic Knee Osteoarthritis Following Arthroscopic Lavage: A Randomized,... by Janette Furuzawa-Carballeda, Guadalupe Lima, Luis Llorente, Carlos Nuñez-Álvarez, Blanca H. Ruiz-Ordaz, Santiago Echevarría-Zuno, Virgilio Hernández-Cuevas

“…Polymerized-type I collagen (polymerized collagen) is a downmodulator of inflammation and cartilage regenerator biodrug. Aim. To evaluate the effect of intraarticular injections of polymerized collagen after arthroscopic lavage on inflammation and clinical improvement in patients with knee osteoarthritis (OA). …”
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A ROS-responsive hydrogel encapsulated with matrix metalloproteinase-13 siRNA nanocarriers to attenuate osteoarthritis progression by Qiuyang Wang, Kai Feng, Guangsheng Wan, Wei Liao, Jing Jin, Peng Wang, Xiaolian Sun, Weijun Wang, Qing Jiang

“…Intra-articular injection of si-Fe-HPP significantly attenuated cartilage degradation in mice with destabilization of the medial meniscus (DMM)-induced OA. …”
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Circular RNA RHOT1 Regulates miR-142-5p/CCND1 to Participate in Chondrocyte Autophagy and Proliferation in Osteoarthritis by Gu Man, Haiwen Yang, Kai Shen, Donghong Zhang, Julin Zhang, Hao Wu, Hongyu Zhang, Jinhuan Wang

“…Meanwhile, the apoptosis was induced in OA rats but the depletion of circRHOT1 could block the phenotype in the rats. The articular cartilage degeneration was promoted in OA rats, while the knockdown of circRHOT1 repressed the degeneration. …”
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Causes of revision hip arthroplasty after hemiarthroplasty for femoral neck fracture by Alexander N. Tsed, Nikita E. Mushtin, Alexander K. Dulaev

“…The use of a larger hemiendoprosthesis cup compared to the femoral head led to early erosion of cartilage tissue. Smaller hemiendoprosthesis cups were complicated by early protrusions of the acetabulum floor. …”
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Bifidobacterium animalis BD400 protects from collagen-induced arthritis through histidine metabolism by Yang Yang, Yang Yang, Qing Hong, Qing Hong, Xuehong Zhang, Zhenmin Liu, Zhenmin Liu

“…Additionally, there was a decrease in osteo-facial fusion and calcified cartilage thickening in the knee joint. Furthermore, the oral administration of B. animalis BD400 resulted in the down-regulation of inflammatory factors TNF-α and collagenase MMP-13 in the knee joint. …”
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Systemic Resolvin E1 (RvE1) Treatment Does Not Ameliorate the Severity of Collagen-Induced Arthritis (CIA) in Mice: A Randomized, Prospective, and Controlled Proof of Concept Study by Rafael Scaf de Molon, Rogier M. Thurlings, Birgitte Walgreen, Monique M. Helsen, Peter M. van der Kraan, Joni Augusto Cirelli, Marije I. Koenders

“…CIA triggered significant inflammation in the synovial cavity, proteoglycan loss, and cartilage and bone destruction in the ankle joints of mice. …”
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Identification of a novel disulfidptosis-related gene signature in osteoarthritis using bioinformatics analysis and experimental validation by Mingjie Wei, Xiangwen Shi, Wenbao Tang, Qian Lv, Yipeng Wu, Yongqing Xu

“…Abstract Osteoarthritis (OA) is a degenerative bone disease characterized by the destruction of joint cartilage and synovial inflammation, involving intricate immune regulation processes. …”
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Microfluidic Synthesis of miR-200c-3p Lipid Nanoparticles: Targeting ZEB2 to Alleviate Chondrocyte Damage in Osteoarthritis by Zheng D, Chen T, Yang K, Yin G, Chen Y, Gui J, Xu C, Lv S

“…Dong Zheng,1,* Tong Chen,2,* Kaiyuan Yang,1 Guangrong Yin,1 Yang Chen,3 Jianchao Gui,4 Chao Xu,1 Songwei Lv5 1Department of Orthopedics, The Affiliated Changzhou No.2 People’s Hospital with Nanjing Medical University, The Third Affiliated Hospital of Nanjing Medical University, Changzhou, People’s Republic of China; 2Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of China; 3Changzhou Productivity Development Center, Changzhou, People’s Republic of China; 4Department of Sports Medicine and Joint Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, People’s Republic of China; 5School of Pharmacy, Changzhou University, Changzhou, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chao Xu; Songwei Lv, Email xuchao_06@163.com; lvsw@cczu.edu.cnIntroduction: Osteoarthritis (OA) is a degenerative joint disease characterized by articular cartilage degeneration. Chondrocyte inflammation, apoptosis, and extracellular matrix degradation accelerated OA progression. …”
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Design, synthesis, structural characterization, in vitro anticancer activity, and in silico studies of some new hydroxylated and fluorinated-substituted hydrazone derivatives by Reşit Çakmak, Eyüp Başaran, Ömer Erdoğan, Suraj N. Mali, Semiha Köprü, Haya Yasin, Shailesh S. Gurav, Giray Topal

“…In this study, novel hydroxylated and fluorinated-substituted hydrazone derivatives bearing an aromatic nitro moiety (2a-d and 3a-d) were designed as potential anticancer drug candidates, synthesized for the first time, and evaluated for their anticancer activity against chondrosarcoma (SW1353), a common primary malignant cartilage-forming tumor, neuroblastoma (SH-SY5Y), a type of brain cancer, and healthy (L929) cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method for 24 h. …”
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The effect of supplementation with type I and type III collagen peptide and type II hydrolyzed collagen on pain, quality of life and physical function in patients with meniscopathy... by Ahmet Serhat Genç, Ali Kerim Yılmaz, Berna Anıl, Esra Korkmaz Salkılıç, Enes Akdemir, Berna Güzel, Ahmet Mor, Hacı Ahmet Yarar, Nizamettin Güzel, Lokman Kehribar

“…Abstract Background Menisci, one of the most important anatomical structures of the knee joint, plays a role in load transfer, stability, shock absorption, prevention of articular cartilage degeneration, and proprioception. Type I collagen, the main component of the meniscus, and type II collagen fibers play an important role in the stability of the knee joint. …”
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Silk Protein Gene Engineering and Its Applications: Recent Advances in Biomedicine Driven by Molecular Biotechnology by Ji X, Li Y, Wang J, Wang G, Ma B, Shi J, Cui C, Wang R

“…By analyzing the genetic factors influencing silk protein expression, this review further elaborates on the innovative applications of silk proteins in drug delivery systems, tissue engineering and regenerative medicine (eg, skin, bone, cartilage, and vascular repair), as well as antibacterial immune strategies. …”
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Identification of biomarkers for knee osteoarthritis through clinical data and machine learning models by Wei Chen, Haotian Zheng, Binglin Ye, Tiefeng Guo, Yude Xu, Zhibin Fu, Xing Ji, Xiping Chai, Shenghua Li, Qiang Deng

“…Abstract Knee osteoarthritis (KOA) represents a progressive degenerative disorder characterized by the gradual erosion of articular cartilage. This study aimed to develop and validate biomarker-based predictive models for KOA diagnosis using machine learning techniques. …”
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Efficient Therapeutic Function and Mechanisms of Human Polyclonal CD8+CD103+Foxp3+ Regulatory T Cells on Collagen-Induced Arthritis in Mice by Juan Sun, Yiming Yang, Xiaona Huo, Beibei Zhu, Zhenhua Li, Xueyu Jiang, Rufeng Xie, Li Gao, Ying Sun, Huahua Fan, Yongming Zhu, Jie Yang

“…In CIA mice, the clinical scores, levels of anti-collagen IgG antibody, and cartilage destruction were significantly reduced after adoptive transfusion with hCD8+Tregs. …”
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Effects of Duhuo Jisheng Decoction on Calcium Homeostasis and Apoptosis of Chondrocytes under Endoplasmic Reticulum Stress by Qing LIN, Wenyi WANG, Danhong PAN, Yanfeng HUANG, Changlong FU, Jinxia YE

“…Objective:To observe the effect of Duhuo Jisheng decoction on the balance of calcium homeostasis and apoptosis of chondrocytes under endoplasmic reticulum stress(ERS), and to preliminarily explore its mechanism of preventing and treating osteoarthritis.Methods:A steady chondrocyte in vitro culture system was established by using 4-week-old SD rat knee cartilage, and identify the third generation chondrocyte by toluidine blue staining.The 25 μmol/L Thapsigargin(TG)was used to induce chondrocyte in vitro culture to produce endoplasmic reticulum stress(ERS), incubate it in Duhuo Jisheng Decoction(100, 200, 400 μg/mL)for 24 hours after induction.The cells were divided into the blank group, the model group, the low-dose Duhuo Jisheng decoction group, the medium-dose Duhuo Jisheng decoction group, and the high-dose Duhuo Jisheng decoction group.Then morphological changes of chondrocytes were observed respectively by inverted microscope, viability of chondrocyte in each group was examined by CCK-8 method, chondrocyte apoptosis was observed by Hoechst 33342 staining and real-time dynamic changes of intracellular Ca²⁺concentration was examined by double turntable laser confocal microscope.Results:①The morphology of chondrocytes: the cells in the blank group grew in a single-layer paving stone shape, while the cells in the model group increased in a polygonal shape, the pseudopodia increased, the gaps widened, and the number of cells decreased significantly.Compared with the model group, the number of cells in the three dose groups of Duhuo Jisheng decoction(100, 200, 400 μg/mL)increased significantly and the cytoplasm was uniform.The activity of chondrocytes in each group: compared with the blank group, the survival rate of the model group was lower, and the difference was statistically significant(<italic>P</italic>&lt;0.05).The absorbance of each concentration group of Duhuo Jisheng decoction increased with the increase of concentration, and the difference was statistically significant compared with the model group(<italic>P</italic>&lt;0.05).③Apoptosis of chondrocytes: the number of apoptosis in the model group was significantly increased, and the degree of pyknosis of the nucleus was obvious, and some of them were a high density of bright blue or half moon-shaped.The number of apoptotic cells in each dose group of Duhuo Jisheng decoction was significantly reduced, only a few apoptotic cells showed a high density of bright blue or half-moon shape, and the number of apoptosis decreased with the increase of the concentration of Duhuo Jisheng decoction, showing a certain dose-effect relationship.④The changes of intracellular calcium ion concentration: the calcium ion fluorescence intensity of the cells in the blank group was weaker and more uniform, maintaining a stable state.The calcium ion release of the model group was significantly increased, and the fluorescence intensity was enhanced.The fluorescence spectrum showed that calcium sparks were more frequent.Compared with the blank group, the difference was statistically significant(<italic>P</italic>&lt;0.05).Chondrocytes in the medium and high-dose Duhuo Jisheng decoction groups could quickly return to calcium homeostasis, and calcium sparks were significantly reduced.Although there were still some calcium sparks in the low-concentration Duhuo Jisheng decoction group, the fluorescence intensity was significantly weaker than the model group.Conclusion:Duhuo Jisheng decoction has an inhibitory effect on the apoptosis of chondrocyte induced by TG, the mechanism may be that Duhuo Jisheng decoction can alleviate endoplasmic reticulum stress by regulating calcium balance, thus inhibiting the apoptosis of chondrocyte.…”
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Integrated bioinformatics analysis and experimental validation of exosome-related gene signature in steroid-induced osteonecrosis of the femoral head by Renqun Mao, Wen Bi, Mengyue Yang, Lei Qin, Wenqing Li

“…Results This study identified twenty DEERGs from the peripheral serum and hip articular cartilage samples of SIONFH patients and HC. Two SIONFH subtypes related to ERGs were identified, and distinctions in pathways and immune cell infiltration patterns were compared. …”
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Immunomodulatory Functions of Mesenchymal Stem Cells in Tissue Engineering by Haojiang Li, Shi Shen, Haitao Fu, Zhenyong Wang, Xu Li, Xiang Sui, Mei Yuan, Shuyun Liu, Guiqin Wang, Quanyi Guo

“…In the application of scaffold, stem cell technology has important applications in cartilage, bone, heart, and liver and other research fields. …”
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Skeletal progenitor LRP1 deficiency causes severe and persistent skeletal defects with Wnt pathway dysregulation by Mohammad Alhashmi, Abdulrahman M. E. Gremida, Santosh K. Maharana, Marco Antonaci, Amy Kerr, Shijian Fu, Sharna Lunn, David A. Turner, Noor A. Al-Maslamani, Ke Liu, Maria M. Meschis, Hazel Sutherland, Peter Wilson, Peter Clegg, Grant N. Wheeler, Robert J. van ‘t Hof, George Bou-Gharios, Kazuhiro Yamamoto

“…Lrp1 deficiency in these stem cells causes joint fusion, malformation of cartilage/bone template and markedly delayed or lack of primary ossification. …”
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Characterization of rectus femoris lesions in knee osteoarthritis at different stages and the effect of ultrasound-guided acupotomy by Wen-Ying Yu, Wen-Ying Yu, Jing Liu, Jing Liu, Ze-Hao Lin, Ze-Hao Lin, Hong Liu, Hong Liu, Liang-Zhi Zhang, Liang-Zhi Zhang, Xiang-Long Feng, Xiang-Long Feng, Zhong-Biao Xiu, Zhong-Biao Xiu, Zhong-Biao Xiu

“…Ultrasoundguided acupotomy operations have been shown to have a protective effect on KOA cartilage and to delay the progression of KOA by ameliorating pathological changes in the rectus femoris muscle. …”
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Finite element investigation for improving chest wall reconstruction process using ceramic and polymeric implants by Yomna H. Shash

“…In this research, customized implants for the ribs and cartilages are used to repair the defective portion of the chest wall. …”
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Development of a Sensitive Quantum Dot-Linked Immunoassay for the Multiplex Detection of Biochemical Markers in a Microvolumeric Format by Kalvaityte U, Bagdonas E, Kirdaite G, Kausaite-Minkstimiene A, Uzieliene I, Ramanaviciene A, Popov A, Butkiene G, Karabanovas V, Denkovskij J, Mobasheri A, Bernotiene E

“…The purpose of the present research was to develop sensitive multiplex detection model of QD-based ELISA (QLISA), through the spectroscopic QD-analyte complex measurements in microvolume liquid droplets on a glass microslide.Methods: QLISA was used for the detection of cartilage oligomeric matrix protein (COMP) and human growth hormone (hGH) as model analytes. …”
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