Protocol for cell image-based spatiotemporal proteomics in budding yeast by Athanasios Litsios, Myra Paz David Masinas, Helena Friesen, Charles Boone, Brenda J. Andrews

“…Here, we present a protocol for the spatiotemporal analysis of the proteome during the budding yeast cell division cycle using live-cell imaging. …”
Get full text

Hormetic Concentrations of Hydrogen Peroxide but Not Ethanol Induce Cross-Adaptation to Different Stresses in Budding Yeast by Halyna M. Semchyshyn

“…In this study, we observed the hormetic-dose response to ethanol in budding yeast S. cerevisiae. Cross-protection is a phenomenon in which exposure to mild stress results in the acquisition of cellular resistance to lethal stress induced by different factors. …”
Get full text

Optical control of sphingolipid biosynthesis using photoswitchable sphingosines by Matthijs Kol, Alexander J.E. Novak, Johannes Morstein, Christian Schröer, Tolulope Sokoya, Svenja Mensing, Sergei M. Korneev, Dirk Trauner, Joost C.M. Holthuis

Subjects: Get full text

Exploring the anti-aging potential of natural products and plant extracts in budding yeast Saccharomyces cerevisiae: A review [version 2; peer review: 1 approved, 2 approved with reservations] by Madhu Dyavaiah, Chella Perumal Palanisamy, Tewin Tencomnao, Siriporn Chuchawankul, Phaniendra Alugoju, Naga Venkata Anusha Anthikapalli, Anchalee Prasanskulab, Selvaraj Jayaraman

“…Biogerontology research aims to explore the molecular basis of aging and discover new anti-aging therapeutic compounds or formulations to combat the detrimental effects of aging and promote a healthy life span. The budding yeast Saccharomyces cerevisiae has been, and continues to be, an indispensable model organism in the field of biomedical research for discovering the molecular basis of aging S. cerevisiae has preserved nutritional signaling pathways (such as the target of rapamycin (TOR)-Sch9 and the Ras-AC-PKA (cAMP-dependent protein kinase) pathways, and shows two distinct aging paradigms chronological life span (CLS) and replicative life span (RLS). …”
Get full text

Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing by Minho Lee, Shin-Jung Choi, Sangjo Han, Miyoung Nam, Dongsup Kim, Dong-Uk Kim, Kwang-Lae Hoe

“…Peculiarly, the frequency of substitutions (67.6%) was unexpectedly higher than in budding yeast (~28%) and well above the predicted error of Sanger sequencing (~2%), which might have arisen during the solid-phase oligonucleotide synthesis and PCR amplification of the barcodes during strain construction. …”
Get full text

THE ORIGIN OF SYNAPTONEMAL COMPLEX PROTEINS. SEARCH FOR RELATED PROTEINS IN PROTEOMES OF ALGAE, LOWER FUNGI, MOSSES, AND PROTOZOANS by T. M. Grishaeva, Yu. F. Bogdanov

“…Proteins similar to known proteins of the synaptonemal complexes (SCs) of seven species of higher eukaryotes, from budding yeast to mouse, which are used as models for studying meiosis, have been sought by bioinformatical methods. …”
Get full text

Mathematical analysis and modeling of DNA segregation mechanisms by Bashar Ibrahim

“…During some asymmetric cell divisions, such as those observed in budding yeast, an additional mechanism, the Spindle Position Checkpoint (SPOC), is required to delay exit from mitosis until the mitotic spindle is correctly aligned. …”
Get full text

AI-assisted diagnosis of vulvovaginal candidiasis using cascaded neural networks by Zhongxiao Wang, Ruliang Wang, Haichun Guo, Qiannan Zhao, Huijun Ren, Jumin Niu, Ying Wang, Wei Wu, Bingbing Liang, Xin Yi, Xiaolei Zhang, Shiqi Xu, Xianling Dong, Liqun Wang, Qinping Liao

“…The model obtained AUC = 0.9447, 0.9711, and 0.9793 for slide-level diagnosing yeast hyphae, budding yeast, and yeast. Compared with the average performance of experts, the Youden indexes of our model’s best points were improved by 0.0069, 0.0772, 0.0579, and 0.0907 for yeast hyphae, budding yeast, yeast, and VVC. …”
Get full text

Proper 5'-3' cotranslational mRNA decay in yeast requires import of Xrn1 to the nucleus. by Antonio Jordán-Pla, Yujie Zhang, José García-Martínez, Shiladitya Chattopadhyay, Anabel Forte, Mordechai Choder, Vicent Pelechano, José E Pérez-Ortín

“…The budding yeast Xrn1 protein shuttles between the nucleus, where it stimulates transcription, and the cytoplasm, where it executes the major cytoplasmic mRNA decay. …”
Get full text

The dependence of shugoshin on Bub1-kinase activity is dispensable for the maintenance of spindle assembly checkpoint response in Cryptococcus neoformans. by Satya Dev Polisetty, Krishna Bhat, Kuladeep Das, Ivan Clark, Kevin G Hardwick, Kaustuv Sanyal

“…Here, we show that shugoshin (Sgo1), a conserved tension-sensing protein, ensures the maintenance of SAC signals in response to unattached kinetochores during mitosis in a basidiomycete budding yeast Cryptococcus neoformans. Sgo1 maintains optimum levels of Aurora B kinase Ipl1 and protein phosphatase 1 (PP1) at kinetochores. …”
Get full text

Blastomycosis Presenting as Multiple Splenic Abscesses: Case Report and Review of the Literature by Sami Al-Nassar, Tracy MacNair, Jeremy Lipschitz, Howard Greenberg, Elly Trepman, Sate Hamza, John M Embil

“…Gomori’s methenamine silver stain showed broad-based budding yeast consistent with Blastomyces dermatitidis. …”
Get full text

An 11-Year-Old Male with Refractory Osteomyelitis by Clifford T. Mauriello, Ole A. Raustol, Maria A. Aguiar, Kenji M. Cunnion

“…The recognition of multifocal osteomyelitis led to reexamination of bone tissue specimens using special stains which demonstrated rare broad-based budding yeast. Fungal cultures eventually grew Blastomyces dermatitidis. …”
Get full text

Fructose-Induced Carbonyl/Oxidative Stress in S. cerevisiae: Involvement of TOR by Bohdana V. Valishkevych, Ruslana A. Vasylkovska, Liudmyla M. Lozinska, Halyna M. Semchyshyn

“…The TOR (target of rapamycin) signaling pathway first described in the budding yeast Saccharomyces cerevisiae is highly conserved in eukaryotes effector of cell growth, longevity, and stress response. …”
Get full text

Spontaneous and environment induced genomic alterations in yeast model by Ke-Jing Li, Lei Qi, Ying-Xuan Zhu, Min He, Qian Xiang, Dao-Qiong Zheng

“…While genomic alterations are fundamental to biological evolution, enabling adaptation and diversity, they can also result in detrimental outcomes, such as the development of genetic diseases including cancer. The budding yeast Saccharomyces cerevisiae serves as an exemplary model for investigating the mechanisms behind various genomic alterations, including point mutations, chromosomal rearrangements, and whole-chromosome aneuploidy. …”
Get full text

Activation of the cell wall integrity pathway promotes escape from G2 in the fungus Ustilago maydis. by Natalia Carbó, José Pérez-Martín

“…For instance, activation of the Cell Wall Integrity (CWI) pathway in the budding yeast Saccharomyces cerevisiae signals an increase in CDK inhibitory phosphorylation, which leads cells to remain in the G2 phase. …”
Get full text

Methylated H3K4, a transcription-associated histone modification, is involved in the DNA damage response pathway. by David Faucher, Raymund J Wellinger

“…Here we show that the mono ubiquitylation of histone H2B and the tri-methylation of histone H3 on lysine 4 (H3K4me3), both known for their involvement in transcription, are also important for a proper response of budding yeast cells to DNA damaging agents and the passage through S-phase. …”
Get full text

Aflatoxin B1-Associated DNA Adducts Stall S Phase and Stimulate Rad51 foci in Saccharomyces cerevisiae by Michael Fasullo, Yifan Chen, William Bortcosh, Minzeng Sun, Patricia A. Egner

“…AFB1 is a potent recombinagen in budding yeast. AFB1 exposure induces RAD51 expression and triggers Rad53 activation in yeast cells that express human CYP1A2. …”
Get full text

Mms22-Rtt107 axis attenuates the DNA damage checkpoint and the stability of the Rad9 checkpoint mediator by Bingbing Wan, Danying Guan, Shibai Li, Tzippora Chwat-Edelstein, Xiaolan Zhao

“…Here, we report that budding yeast employs a pathway composed of the scaffold protein Rtt107, its binding partner Mms22, and an Mms22-associated ubiquitin ligase complex to downregulate the DNA damage checkpoint. …”
Get full text

A Case of Onychomycosis Caused by Rhodotorula glutinis by Hatice Uludag Altun, Tuba Meral, Emel Turk Aribas, Canan Gorpelioglu, Nilgun Karabicak

“…The causative agent was identified as R. glutinis based on the pinkish-orange color; mucoid-appearing yeast colonies on Sabouraud Dextrose Agar at 25°C; morphological evaluation in the Corn Meal-Tween 80 agar; observed oval/round budding yeast at 25°C for 72 hours; no observed pseudohyphae; positive urease activity at 25°C for 4 days; and assimilation features detected by API ID 32C kit and automated Vitek Yeast Biochemical Card 2 system. …”
Get full text

Pooled PPIseq: Screening the SARS-CoV-2 and human interface with a scalable multiplexed protein-protein interaction assay platform. by Darach Miller, Adam Dziulko, Sasha Levy

“…This approach combines the mDHFR split-tag reporter with the iSeq2 interaction-barcoding system to permit massively-multiplexed PPI quantification by simple pooled engineering of barcoded constructs, integration of these constructs into budding yeast, and fitness measurements by pooled cell competitions and barcode-sequencing. …”
Get full text