Enhanced fetal hemoglobin production via dual-beneficial mutation editing of the HBG promoter in hematopoietic stem and progenitor cells for β-hemoglobinopathies by Prathibha Babu Chandraprabha, Manoj Kumar K. Azhagiri, Vigneshwaran Venkatesan, Wendy Magis, Kirti Prasad, Sevanthy Suresh, Aswin Anand Pai, Srujan Marepally, Alok Srivastava, Kumarasamypet Murugesan Mohankumar, David I. K. Martin, Saravanabhavan Thangavel

“…Further support through bone marrow MSC co-culture boosted complete HDR conversion rates to exceed 80%, with minimal InDels, improved cell viability, and induced fetal hemoglobin levels similar to those of Cas9 RNP-mediated indels at BCL11A enhancer and HBG promoter. …”
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Management of CAR-T cell therapy in patients with multiple myeloma: a systematic review and expert consensus in Australia by P. Joy Ho, Hang Quach, M. Hasib Sidiqi, M. Hasib Sidiqi, Cindy H. Lee, Jason Butler, Andrew Spencer, Kenneth Micklethwaite, Kenneth Micklethwaite, Kenneth Micklethwaite, Kenneth Micklethwaite, Jingya Li, Elissa Cusson, Robert Bagnall, Simon J. Harrison

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Human Periodontal Ligament Stem Cell-Derived Exosomes Promote Bone Regeneration by Altering MicroRNA Profiles by Ting Liu, Wenyun Hu, Xue Zou, Jingchen Xu, Shushu He, Le Chang, Xinyi Li, Yuanyuan Yin, Mi Tian, Ziyu Li, Jialiang Zhou, Xiaoge Jiang, Song Chen

“…In the present study, we identified the exosomes derived from PDLSCs and found that osteogenic induction can enhance the osteogenic ability of PDLSC-derived exosomes in promoting the osteogenic differentiation of rat bone marrow stem cells (BMSCs). To investigate the underlying mechanism, we analyzed the exosomal miRNA expression profiles of undifferentiated and osteogenic differentiated PDLSCs by RNA sequencing. …”
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Lipoxin A4 yields an electrophilic 15-oxo metabolite that mediates FPR2 receptor-independent anti-inflammatory signaling by Adolf Koudelka, Gregory J. Buchan, Veronika Cechova, James P. O’Brien, Emily R. Stevenson, Crystal E. Uvalle, Heng Liu, Steven R. Woodcock, Steven J. Mullett, Cheng Zhang, Bruce A. Freeman, Stacy L. Gelhaus

“…The generation and signaling actions of LXA4 and its primary 15-oxo metabolite were assessed in control, lipopolysaccharide-activated, and arachidonic acid-supplemented RAW264.7 and bone marrow-derived macrophages. Despite the expression of catalytically active enzymes required for LXA4 synthesis, both LXA4 and its 15-oxo-LXA4 metabolite were undetectable in all conditions. …”
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Dual-sided centripetal microgrooved poly (D,L-lactide-co-caprolactone) disk encased in immune-regulating hydrogels for enhanced bone regeneration by You Wu, Xiaokun Yue, Ying Zhang, Ning Yu, Chengyan Ge, Rui Liu, Zhongying Duan, Lilong Gao, Xinlong Zang, Xin Sun, Deteng Zhang

“…Bone regeneration-related cells (e.g., bone marrow mesenchymal stem cells, macrophages, Schwann cells, and endothelial cells) cultured on PLCL films tended to align along the stripes and migrate from the periphery toward the center region in vitro. …”
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Implantable Multifunctional Micro‐Oxygen Reservoir System for Promoting Vascular‐Osteogenesis via Remodeling Regenerative Microenvironment by Min Rui, Jiannan Mao, Hongshuai Wu, Yujian Hui, Hao Shen, Yilin Yang, Tao Ma, Kewei Ren, Juan Wang, Wenguo Cui, Qin Shi, Huilin Yang

“…The CSP‐GM with extra cellular matrix (ECM)‐mimicking structures reconstructed living niches, promoting the adhesion and proliferation of bone marrow stromal cells (BMSCs). As a multifaceted microenvironment regulator, CSP‐GM remodeled the regenerative microenvironment by synergistically producing oxygen and scavenging ROS, recovering mitochondrial homeostasis and antioxidant defenses of BMSCs, promoting angiogenesis and osteogenesis under hypoxia conditions via precisely modulating the Nrf2/HO‐1 signaling pathway. …”
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In vitro antitumor and immunomodulatory activities of 1,2,4-oxadiazole derivatives by Héverton Mendes Araújo, Gabriel Acácio de Moura, Yasmim Mendes Rocha, Cristian Vicson Pinheiro Gomes, Valentina Nascimento e Melo de Oliveira, Ronaldo Nascimento de Oliveira, Larissa Deadame de Figueiredo Nicolete, Emanuel Paula Magalhães, Ramon R.P.P.B. de Menezes, Roberto Nicolete

“…Thus, the present study aims to evaluate the antitumor and immunomodulatory effects of the synthetic derivative 1,2,4-oxadiazole, N-cyclohexyl-3-(3-methylphenyl)-1,2,4-oxadiazole-5-amine (1,2,4-oxadiazole derivative 2), in melanoma cells and murine Bone Marrow-Derived Macrophages (BMDMs). Cytotoxicity in B16–F10 and BMDMs cells was assessed using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) MTT method. 1,2,4-oxadiazole derivative 2 exhibited antiproliferative effects on both cell lines, being 2.6 times more selective for B16–F10. …”
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The Human Milk-derived Peptide Drives Rapid Regulation of Macrophage Inflammation Responses in the Neonatal IntestineSummary by Fuqiang Yuan, Xu Han, Masha Huang, Yinglin Su, Yiting Zhang, Mengyuan Hu, Xiang Yu, Weilai Jin, Yun Li, Le Zhang

“…Immunofluorescence analysis was used to determine its distribution, and its impact on NF-κB signaling and inflammation was studied in bone marrow-derived macrophages (BMDMs) and intestinal macrophages. …”
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Brucella osteoarthritis: recent progress and future directions by Jinlei Chen, Jinlei Chen, Jinlei Chen, Jinlei Chen, Jinlei Chen, Feijie Zhi, Feijie Zhi, Feijie Zhi, Guanghai Zhao, Guanghai Zhao, Mengru Su, Mengru Su, Mengru Su, Hao Geng, Hao Geng, Hao Geng, Wei Song, Wei Song, Yuefeng Chu, Yuefeng Chu, Yuefeng Chu, Haihong Zhang, Haihong Zhang

“…A variety of in vitro and in vivo models have been employed to study Brucella osteoarthritis, such as using bone marrow-derived macrophages to establish cell models and mice to develop animal models of Brucella osteoarthritis. …”
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Leishmania mexicana: Novel Insights of Immune Modulation through Amastigote Exosomes by Laura Enedina Soto-Serna, Mariana Diupotex, Jaime Zamora-Chimal, Adriana Ruiz-Remigio, José Delgado-Domínguez, Rocely Buenaventura Cervantes-Sarabia, Adriana Méndez-Bernal, Alma Reyna Escalona-Montaño, María Magdalena Aguirre-García, Ingeborg Becker

“…The incubation of aExo with bone marrow-derived macrophages (BMMs) infected with L. mexicana led to their internalization and were found to colocalize with the cellular tetraspanin CD63. …”
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Activated B Lymphocyte Inhibited the Osteoblastogenesis of Bone Mesenchymal Stem Cells by Notch Signaling by Mengxue Pan, Wei Hong, Ye Yao, Xiaoxue Gao, Yi Zhou, Guoxiang Fu, Yuanchuang Li, Qiang Guo, Xinxin Rao, Peiyuan Tang, Shengzhi Chen, Weifang Jin, Guoqiang Hua, Jianjun Gao, Xiaoya Xu

“…Additionally, changes in lymphocytes (B lymphocyte, CD4+ and CD8+ T lymphocytes, and macrophages) in the bone marrow were analyzed by flow cytometry. The osteogenesis of BMSCs cocultured with normal and LPS-pretreated B lymphocytes was detected by BCIP/NBT and Alizarin red S staining. …”
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Neuronal Transdifferentiation Potential of Human Mesenchymal Stem Cells from Neonatal and Adult Sources by a Small Molecule Cocktail by Lorena V. Cortés-Medina, Herminia Pasantes-Morales, Alejandro Aguilera-Castrejon, Arturo Picones, Cesar O. Lara-Figueroa, Enoch Luis, Juan Jose Montesinos, Victor A. Cortés-Morales, M. P. De la Rosa Ruiz, Erika Hernández-Estévez, Laura C. Bonifaz, Marco Antonio Alvarez-Perez, Gerardo Ramos-Mandujano

“…MSCs can be obtained from many different sources, and the present study compares the potential of neuronal transdifferentiation in MSCs from adult and neonatal sources (Wharton’s jelly (WhJ), dental pulp (DP), periodontal ligament (PDL), gingival tissue (GT), dermis (SK), placenta (PLAC), and umbilical cord blood (UCB)) with a protocol previously tested in bone marrow- (BM-) MSCs consisting of a cocktail of six small molecules: I-BET151, CHIR99021, forskolin, RepSox, Y-27632, and dbcAMP (ICFRYA). …”
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Stable Gastric Pentadecapeptide BPC 157 as Therapy After Surgical Detachment of the Quadriceps Muscle from Its Attachments for Muscle-to-Bone Reattachment in Rats by Danijel Matek, Irena Matek, Eva Staresinic, Mladen Japjec, Ivan Bojanic, Alenka Boban Blagaic, Lidija Beketic Oreskovic, Ivana Oreskovic, Tihomil Ziger, Tomislav Novinscak, Ivan Krezic, Sanja Strbe, Martin Drinkovic, Filip Brkic, Jelena Popic, Anita Skrtic, Sven Seiwerth, Mario Staresinic, Predrag Sikiric, Ivica Brizic

“…Consequently, at 3 months, the form was stable, and the balance between the muscle and bone was the following: well-organized bone, newly formed as more cortical bone providing a narrower bone marrow space, and the muscle and mature fibers were oriented parallel to the bone axis and were in close contact with bone. …”
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Gambaran Hitung Jenis Leukosit pada Pasien Penyakit Paru Obstruktif Kronik yang Dirawat di RSUP Dr. M. Djamil Padang by Revi Sofiana Martantya, Ellyza Nasrul, Masrul Basyar

“…Cigarette smoke, air pollution, and recurrent infections in the respiratory tract can activates alveolar macrophages to release inflammatory mediators that stimulate granulocytic and monocytic progenitors in the bone marrow that can affect leukocyte counts in peripheral blood. …”
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Harnessing genomic and bioinformatic data to broaden understanding of leukaemia across continents by Gumelar Gugun, Ulfa Mia Maria, Amukti Danang Prasetyaning, Irham Lalu Muhammad, Yuliani Sapto, Adikusuma Wirawan, Khairi Sabiah, Darmawi Darmawi, Chong Rockie, Ates Ilker, Singh Dilpreet, Chavan Aditya Ashok

“…Background/Aim: Leukaemia is a malignant disease of blood cells found in the bone marrow, which can be divided into acute lymphocytic leukaemia and myelocytic leukaemia. …”
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Generation and miRNA Characterization of Equine Induced Pluripotent Stem Cells Derived from Fetal and Adult Multipotent Tissues by Laís Vicari de Figueiredo Pessôa, Pedro Ratto Lisboa Pires, Maite del Collado, Naira Caroline Godoy Pieri, Kaiana Recchia, Aline Fernanda Souza, Felipe Perecin, Juliano Coelho da Silveira, André Furugen Cesar de Andrade, Carlos Eduardo Ambrosio, Fabiana Fernandes Bressan, Flavio Vieira Meirelles

“…Adult equine fibroblasts (eFibros) and mesenchymal cells derived from the bone marrow (eBMmsc), adipose tissue (eADmsc), and umbilical cord tissue (eUCmsc) were isolated, their multipotency was characterized, and the cells were induced in vitro into pluripotency (eiPSCs). eiPSCs were generated through a lentiviral system using the factors OCT4, SOX2, c-MYC, and KLF4. …”
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Measurable residual disease as predictor of post–day +100 relapses after allografting in adult AML by Naveed Ali, Megan Othus, Eduardo Rodríguez-Arbolí, Corentin Orvain, Filippo Milano, Brenda M. Sandmaier, Chris Davis, Ryan S. Basom, Frederick R. Appelbaum, Roland B. Walter

“…To examine the role of MFC MRD testing to predict later relapses, we examined 935 adults with AML or myelodysplastic neoplasm/AML transplanted in first or second morphologic remission who underwent bone marrow restaging studies between day 70 and 100 after HCT and were alive and without relapse by day +100. …”
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Arginase-1-specific T cells target and modulate tumor-associated macrophages by Mads Hald Andersen, Inés Lecoq, Ayako Wakatsuki Pedersen, Evelina Martinenaite, Shamaila Munir Ahmad, Maria Perez-Penco, Lucia Lara de la Torre, Marion Chapellier, Lars Rønn Olsen, Mia Aaboe-Jørgensen, Hannah Jorinde Glöckner, Anne Mette Askehøj Rømer

“…In the present study, we investigated how Arg1-specific T cells can directly target TAMs and influence their polarization.Methods Murine Arg1-specific CD4+T cells isolated from splenocytes of animals vaccinated with an Arg1-derived peptide in the adjuvant montanide were co-cultured with either in vitro M2-differentiated bone marrow-derived macrophages or ex vivo isolated F4/80+TAMs. …”
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TNF-α Induced the Enhanced Apoptosis of Mesenchymal Stem Cells in Ankylosing Spondylitis by Overexpressing TRAIL-R2 by Zhenhua Liu, Liangbin Gao, Peng Wang, Zhongyu Xie, Shuizhong Cen, Yuxi Li, Xiaohua Wu, Le Wang, Hongjun Su, Wen Deng, Shan Wang, Deng Li, Jinteng Li, Yi Ouyang, Yanfeng Wu, Huiyong Shen

“…The present study aims to assess the apoptosis of bone marrow-derived ASMSCs and to investigate the underlying mechanisms of altered ASMSCs apoptosis. …”
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Sporadic Burkitt Lymphoma First Presenting as Painful Gingival Swellings and Tooth Hypermobility: A Life-Saving Referral by Erofili Papadopoulou, Maria Kouri, Dimitrios Velonis, Anastasia Andreou, Maria Georgaki, Spyridon Damaskos, Evangelia Piperi, Konstantina Delli, Ioannis K. Karoussis, Antonia Vlachou, Georgia Avgerinou, Antonis Kattamis, Nikolaos G. Nikitakis

“…The patient underwent a full staging work-up, revealing bone marrow involvement and widespread disease. A multi-chemotherapy regimen was initiated with the regression of oral lesions and symptoms within a few weeks and complete disease remission after nine chemotherapy cycles. …”
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