Enhanced fetal hemoglobin production via dual-beneficial mutation editing of the HBG promoter in hematopoietic stem and progenitor cells for β-hemoglobinopathies by Prathibha Babu Chandraprabha, Manoj Kumar K. Azhagiri, Vigneshwaran Venkatesan, Wendy Magis, Kirti Prasad, Sevanthy Suresh, Aswin Anand Pai, Srujan Marepally, Alok Srivastava, Kumarasamypet Murugesan Mohankumar, David I. K. Martin, Saravanabhavan Thangavel

“…Further support through bone marrow MSC co-culture boosted complete HDR conversion rates to exceed 80%, with minimal InDels, improved cell viability, and induced fetal hemoglobin levels similar to those of Cas9 RNP-mediated indels at BCL11A enhancer and HBG promoter. …”
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Single-arm multicenter phase II study on aggressive local consolidative therapy in combination with systemic chemotherapy for stage IV non-small cell lung carcinoma with oligometas... by Takaaki Tokito, Kazuhiko Yamada, Hidenobu Ishii, Yuichi Takiguchi, Go Saito, Koichi Minato, Hisao Imai, Hiroshi Tanaka, Satoru Miura, Kageaki Watanabe, Yoshifusa Koreeda, Akira Ono, Naoki Furuya, Toshihiro Misumi, Kazushige Hayakawa, Etsuyo Ogo, Hiroaki Okamoto

“…The metastasis sites included the brain, bone, adrenal gland, lung, and cervical lymph node (n = 9, 7, 2, 1, and 1, respectively). …”
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Establishment of a Southern catfish (Silurus meridionalis) spermatogonial stem cell line capable of sperm production in vitro by Jianeng Li, Changle Zhao, Lei Liu, Shuqing Zheng, Deshou Wang, Changwei Shao, Jing Wei

“…Southern catfish (Silurus meridionalis) is widely distributed in the Yangtze River and its tributaries and is valued for its rapid growth, nutritional content, pleasant taste, absence of intermuscular bone, and disease resistance. However, the species faces challenges in genetic breeding and germ-line preservation due to its late sexual maturity (2–3 years for males and 3–4 years for females) and single spawning cycle. …”
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Single-cell profiling of SLC family transporters: uncovering the role of SLC7A1 in osteosarcoma by Yan Liao, Junkai Chen, Hao Yao, Ting Zheng, Jian Tu, Weidong Chen, ZeHao Guo, Yutong Zou, Lili Wen, Xianbiao Xie

“…Abstract Background Osteosarcoma is the most common malignant bone tumor in children and adolescents, characterized by high disability and mortality rates. …”
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The Role of EGFR/PI3K/Akt/cyclinD1 Signaling Pathway in Acquired Middle Ear Cholesteatoma by Wei Liu, Hongmiao Ren, Jihao Ren, Tuanfang Yin, Bing Hu, Shumin Xie, Yinghuan Dai, Weijing Wu, Zian Xiao, Xinming Yang, Dinghua Xie

“…Cholesteatoma is a benign keratinizing and hyper proliferative squamous epithelial lesion of the temporal bone. Epidermal growth factor (EGF) is one of the most important cytokines which has been shown to play a critical role in cholesteatoma. …”
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B7-H3 CAR-T cell therapy combined with irradiation is effective in targeting bulk and radiation-resistant chordoma cancer cells by Kun Wang, Gianpietro Dotti, Soldano Ferrone, Yufeng Wang, Yin P Hung, Tao Ma, Giulia Cattaneo, Xinhui Wang, David O Osei-Hwedieh, Tara A Walhart, Joseph H Schwab

“…Background Chordoma is a slow-growing, primary malignant bone tumor that arises from notochordal tissue in the midline of the axial skeleton. …”
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Immunotherapy combined with chemotherapy without locoregional radiotherapy in de novo metastatic nasopharyngeal carcinoma: two case reports and a literature review by Yingna Gao, Xiaoqiong Shi, Jianqiao He, Hui Yao, Guoning Yu, Lin Zhao, Yi Ma, Yi Ma, Hongliang Zheng, Minhui Zhu, Caiyun Zhang

“…Radiological examination with positron emission tomography–computed tomography (PET-CT) and contrast-enhanced magnetic resonance imaging (MRI) showed NPC with multiple systemic lymph node metastases, multiple bone metastases, and liver metastases. Both patients were diagnosed with dmNPC and received pabolizumab in combination with six courses of platinum-based chemotherapy treatment. …”
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A Novel Therapeutic Calcium Peroxide Loaded Injectable Bio-adhesive Hydrogel Against Periodontitis by Shaojie Dong, Yukun Mei, Yuwei Zhang, Wenqing Bu, Yifei Zhang, Changjie Sun, Rui Zou, Lin Niu

“…Furthermore, the cytotoxicity, osteogenic ability, antibacterial behavior, and alveolar bone regenerating capability of CP-P hydrogel were conducted both in vitro and in vivo. …”
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LncRNA PTS-1 Protects Against Osteoarthritis Through the miR-8085/E2F2 Axis by Ma C, Chen Q, Wei YF, Chen SW, Liu H, Xin F, Ren YX

“…Cheng Ma,1,* Qi Chen,2,* Yi-Fan Wei,1,* Shu-Wen Chen,3 Huan Liu,4 Feng Xin,5 Yong-Xin Ren1 1Department of Orthopaedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, People’s Republic of China; 2Department of Orthopaedics, The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical University, Changzhou, Jiangsu, People’s Republic of China; 3Department of Clinical College, The First Clinical School of Nanjing Medical University, Nanjing, Jiangsu, People’s Republic of China; 4Department of Orthopaedics, The Affiliated Huai’an No.1 People’s Hospital of Nanjing Medical University, Huai’an, Jiangsu, People’s Republic of China; 5Department of Orthopaedics, Xuzhou Cancer Hospital, Xuzhou, Jiangsu, 221005, People’s Republic of China*These authors contributed equally to this workCorrespondence: Feng Xin; Yong-Xin Ren, Email 925866554@qq.com; renyongxinjsph@163.comBackground: Osteoarthritis (OA) is a leading cause of pain, disability, and reduced mobility worldwide, characterized by metabolic imbalances in chondrocytes, extracellular matrix (ECM), and subchondral bone. Emerging evidence highlights the critical role of long non-coding RNAs (lncRNAs) in OA pathogenesis. …”
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Immunosenescence and its related comorbidities in older people living with HIV by Xiao-Yan He, Xiu-Wen Wang, Shuang Li, Na Li, Wei Li, Yan Hu, Na Guo, Yong-Tang Zheng, Hong-Yi Zheng, Bin Su, Haijuan Wang

“…Although antiretroviral therapy has demonstrated positive effects in OPLWH, concerns persist regarding overall poor immune reconstitution and elevated rates of age-related comorbidities, such as cardiovascular disease, bone disease, and cognitive impairment. This review aims to elucidate the mechanisms underlying immunosenescence and the interaction of immunosenescence with HIV infection, further exploring its role in the pathogenesis of HIV infection during aging. …”
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Finite element investigation for improving chest wall reconstruction process using ceramic and polymeric implants by Yomna H. Shash

“…After excision, artificial bone or synthetic materials are used in chest wall reconstruction to restore the skeletal structure of the chest. …”
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In-vitro and in-vivo assessment of biocompatibility and efficacy of ostrich eggshell membrane combined with platelet-rich plasma in Achilles tendon regeneration by Omid Koohi-Hosseinabadi, Reza Shahriarirad, Amireza Dehghanian, Laleh Amini, Sajjad Barzegar, Afrooz Daneshparvar, Omid Alavi, Seyedeh-Parvin Khazraei, Saba Hosseini, Ali Arabi Monfared, Roya Khorram, Nader Tanideh, Soheil Ashkani-Esfahani

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Sustainability status of amphidromous nike fish, postlarva Gobioidei, in estuarine water by F.M. Sahami, S.N. Hamzah, A.H. Tome, S.A. Habibie, M.R.U. Puluhulawa

“…Therefore, this study assessed the sustainability status of Nike fish in the waters of Tomini Bay Gorontalo.METHODS: This study was conducted across five Nike fishing locations in Tomini Bay, Gorontalo Province, namely the Bone-Bolango, Bilungala, Tombulilato, Taludaa, and Bilato estuaries, from April to September 2023. …”
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Management of CAR-T cell therapy in patients with multiple myeloma: a systematic review and expert consensus in Australia by P. Joy Ho, Hang Quach, M. Hasib Sidiqi, M. Hasib Sidiqi, Cindy H. Lee, Jason Butler, Andrew Spencer, Kenneth Micklethwaite, Kenneth Micklethwaite, Kenneth Micklethwaite, Kenneth Micklethwaite, Jingya Li, Elissa Cusson, Robert Bagnall, Simon J. Harrison

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Lipoxin A4 yields an electrophilic 15-oxo metabolite that mediates FPR2 receptor-independent anti-inflammatory signaling by Adolf Koudelka, Gregory J. Buchan, Veronika Cechova, James P. O’Brien, Emily R. Stevenson, Crystal E. Uvalle, Heng Liu, Steven R. Woodcock, Steven J. Mullett, Cheng Zhang, Bruce A. Freeman, Stacy L. Gelhaus

“…The generation and signaling actions of LXA4 and its primary 15-oxo metabolite were assessed in control, lipopolysaccharide-activated, and arachidonic acid-supplemented RAW264.7 and bone marrow-derived macrophages. Despite the expression of catalytically active enzymes required for LXA4 synthesis, both LXA4 and its 15-oxo-LXA4 metabolite were undetectable in all conditions. …”
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Compassionate Use of Omadacycline in a Down Syndrome Pre-Schooler With Critically Ill Atypical Pneumonia Caused by Macrolide-Resistant Mycoplasma Pneumoniae by Wu D, Xie FJ, Wang YJ, Jiang XH, Zhang GL, Zhang H, Zhu YC, Zhang Y, Tang YJ, Lin YL, Xu JX, Zhang JN, Liu BW, Kang K, Gao Y

“…At present, OMC is not recommended in the package insert for clinical application in pediatric patients under 8 years of age due to potential age-specific AEs on tooth colour and development as well as bone growth. The superior efficacy and safety of OMC in the management of critically ill atypical pneumonia caused by macrolide-resistant MP were comprehensively documented in this Down syndrome pre-schooler, which merits future well-designed studies to validate our findings, enhance understanding of the features of OMC, and further expand its clinical application in preschool-aged patients.Keywords: omadacycline, pre-schooler, down syndrome, macrolide-resistant mycoplasma pneumoniae, atypical pneumonia, targeted next generation sequencing, aetiological identification, antimicrobial resistance gene detection…”
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In vitro antitumor and immunomodulatory activities of 1,2,4-oxadiazole derivatives by Héverton Mendes Araújo, Gabriel Acácio de Moura, Yasmim Mendes Rocha, Cristian Vicson Pinheiro Gomes, Valentina Nascimento e Melo de Oliveira, Ronaldo Nascimento de Oliveira, Larissa Deadame de Figueiredo Nicolete, Emanuel Paula Magalhães, Ramon R.P.P.B. de Menezes, Roberto Nicolete

“…Thus, the present study aims to evaluate the antitumor and immunomodulatory effects of the synthetic derivative 1,2,4-oxadiazole, N-cyclohexyl-3-(3-methylphenyl)-1,2,4-oxadiazole-5-amine (1,2,4-oxadiazole derivative 2), in melanoma cells and murine Bone Marrow-Derived Macrophages (BMDMs). Cytotoxicity in B16–F10 and BMDMs cells was assessed using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) MTT method. 1,2,4-oxadiazole derivative 2 exhibited antiproliferative effects on both cell lines, being 2.6 times more selective for B16–F10. …”
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The Human Milk-derived Peptide Drives Rapid Regulation of Macrophage Inflammation Responses in the Neonatal IntestineSummary by Fuqiang Yuan, Xu Han, Masha Huang, Yinglin Su, Yiting Zhang, Mengyuan Hu, Xiang Yu, Weilai Jin, Yun Li, Le Zhang

“…Immunofluorescence analysis was used to determine its distribution, and its impact on NF-κB signaling and inflammation was studied in bone marrow-derived macrophages (BMDMs) and intestinal macrophages. …”
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SaaS sRNA promotes the interfering effect of Salmonella on hepatic iron metabolism via modulating ferroportin 1 by Linlin Cai, Yunting Xie, Haijing Hu, Chongyang Lv, Liangting Shao, Huhu Wang, Xinglian Xu, Guanghong Zhou

“…Utilizing a combined BALB/c mouse model and HepG2 cell model, we demonstrated that SaaS enhances hepcidin synthesis via the bone morphogenetic protein (BMP)-SMAD pathway, leading to decreased ferroportin 1 level. …”
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Efficacy of the Bushen Jianpi Huoxue Formula on Beclin-1/Bcl-2-mediated autophagy and apoptosis in osteoblasts by Yanping Lin, Yanping Lin, Rui Zhao, Rui Zhao, Jiachun Huang, Tongying Chen, Tongying Chen, Haolin Yang, Haolin Yang, Haiwei Guo, Lei Wan, Zhihai Zhang, Ying Li, Genfu Zhu, Hongxing Huang, Hongxing Huang

“…This underscores the formula’s multifaceted role in promoting bone health and managing cellular stress, and offers novel insights into its therapeutic potential against osteoporosis.…”
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