Integration analysis of microRNAs as potential biomarkers in early-stage lung adenocarcinoma: the diagnostic and therapeutic significance of miR-183-3p by Guodong Huang, Yuxia Liu, Lisha Li, Bing Li, Ting Jiang, Yufeng Cao, Xiaoping Yang, Xinning Liu, Honglin Qu, Shitao Li, Xin Zheng

“…The clinical relevance target genes of miR-183-3p were predicted by TargetScan databases and bioinformatics assays. Gene-specific experimental validation was performed using qRT-PCR, western blotting and luciferase reporter assays.ResultsWe identified 36 differentially expressed miRNAs between LUAD tissues and matched paracancerous tissues. …”
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An integrative analysis of ASCL1 in breast cancer and inhibition of ASCL1 increases paclitaxel sensitivity by activating ferroptosis via the CREB1/GPX4 axis by Xiaolu Yang, Xiaolu Yang, Yilun Li, Yilun Li, Yaqi Peng, Yuan Chang, Binglu He, Binglu He, Tianqi Zhang, Tianqi Zhang, Shiyu Zhang, Cuizhi Geng, Yunjiang Liu, Xiaolong Li, Jun Hao, Li Ma

“…This study aims to elucidate the function of ASCL1 in BC using bioinformatics analyses, as well as in vitro and in vivo experimental approaches.MethodsData from the TCGA, GEO, and Human Protein Atlas databases were utilized to evaluate ASCL1 expression in BC and its association with patient prognosis. …”
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Network and systems biology approaches help investigate gene regulatory interactions between Salmonella disease and host in chickens: Model‐based in silico evidence combined with g... by Reza Tohidi, Hoda Javaheri Bargourooshi, Arash Javanmard

“…To start, we used free computational tools and online bioinformatics resources, including predicting gene function using a multiple association network integration algorithm (geneMania), the Kyoto Encyclopedia of Genes and Genomes, the Annotation, Visualization and Integrated Discovery (DAVID) database and the stimulator of interferon genes. …”
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CircSATB2 modulates fear extinction memory via Robo3-driven synaptic plasticity by Ziyue Xu, Jichun Shi, Runming Liu, Zhehao Li, Shuangxiang Xu, Hao Gong, Mingyue Fu, Hongyu Xu, Shuangqi Yu, Junhui Liu, Huiqing Wu, Xiang Li, Sha Liu, Wei Wei

“…RNA sequencing (RNA-seq) and bioinformatics analyses revealed that circSatb2 enhances the transcription of Roundabout Guidance Receptor 3 (Robo3), a key gene implicated in axon guidance and synaptic plasticity, which was validated by RT-qPCR. …”
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Arginase-1-specific T cells target and modulate tumor-associated macrophages by Mads Hald Andersen, Inés Lecoq, Ayako Wakatsuki Pedersen, Evelina Martinenaite, Shamaila Munir Ahmad, Maria Perez-Penco, Lucia Lara de la Torre, Marion Chapellier, Lars Rønn Olsen, Mia Aaboe-Jørgensen, Hannah Jorinde Glöckner, Anne Mette Askehøj Rømer

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Modern perspective of the Rice Diet for hypertension and other metabolic diseases by Anthony Kuo, Pao-Hwa Lin, Yi-Ju Li, Friedrich C Luft, David López, Romeo Sommerfeld, Paul Ermler, Jana Fehr, Benjamin Bergner, Scott Sanoff, Francis Neelon, William McDowell, Smilla Fox, Abdullatif Ghajar, Elena Gensch, Cedric Lorenz, Martin Preiss, Tom Richter, Philip Klemmer, Anastacia Bohannon, Christoph Lippert

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SEC61 translocon gamma subunit is correlated with glycolytic activity, epithelial mesenchymal transition and the immune suppressive phenotyp... by Zhou Changshuai, Cui Huanhuan, Yang Yuechao, Chen Lei, Feng Mingtao, Gao Yang, Li Deheng, Li Liangdong, Chen Xin, Li Xiaoqiu, Cao Yiqun

“…In this study, we use a multifaceted approach, combining bioinformatics analysis with experimental validation, to elucidate the pivotal role of SEC61G and its associated molecular mechanisms in LUAD. …”
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Resistome phylodynamics of multidrug-resistant Shigella isolated from diarrheal patients by Asaduzzaman Asad, Md. Abu Jaher Nayeem, Md. Golam Mostafa, Ruma Begum, Shah Nayeem Faruque, Suraia Nusrin, Israt Jahan, Shoma Hayat, Zhahirul Islam

“…Whole-genome sequencing (WGS) and bioinformatics approaches were performed to unveil the resistome dynamics: >500 global plasmid entities and >1,000 plasmid-mediated resistance gene clusters from global databases were included in this study. …”
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Treatment of pancreatic cancer with irreversible electroporation and intratumoral CD40 antibody stimulates systemic immune responses that inhibit liver metastasis in an orthotopic... by Partha Ray, Tomoko Hayashi, Karen Messer, Stephen Schoenberger, Aaron Miller, Dennis Carson, Sara McArdle, Zbigniew Mikulski, Jayanth S Shankara Narayanan, Suna Erdem, Herve Tiriac, Minya Pu, Rebekah R White

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Possible scenarios for the spread of mpox outside the endemic focus in Africa by Eskild Petersen, Ulrik Hvid, Oyewale Tomori, Anders Gorm Pedersen, Jacco Wallinga, Richard Pebody, Orlando Cenciarelli, Preben Aavitsland, David Van Laeken, Viggo Andreasen, Uffe Schneider, Julia Kinane Simonsen, Marlies Jilles Francine Goedknegt, Caroline Klint Johannesen, Jens D. Lundgren, Anders Koch, Bolette Søborg, Anna Mia Ekström, Hannah Nohynek, Frank M. Aarestrup, Tyra G. Krause, Lone Simonsen

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Co-isolation of human donor eye cells and development of oncogene-mutated melanocytes to study uveal melanoma by Yunxi Chen, Eva Jin, Mohamed Abdouh, Éric Bonneil, Daniel Alexander Jimenez Cruz, Thupten Tsering, Qianqian Zhou, Aurélie Fuentes-Rodriguez, Alexandra Bartolomucci, Alicia Goyeneche, Solange Landreville, Miguel N. Burnier, Julia V. Burnier

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ELABELA promotes the migration and homing of bone marrow mesenchymal stem cells to myocardial injury sites through the ERK1/2/miR-299a-5p/Exo70 pathway by Jing-Yu Hou, Hao Wu, Shuang-Mei Li, Xiao-Jing Li, Shu-Jun Yang, Xu-Xiang Chen, Chang-Qing Zhou, Hui-Bao Long, Hai-Dong Wu, Jia-Ying Fu, Ya-Jie Guo, Tong Wang

“…Transcriptome sequencing and bioinformatics analysis were employed to identify and validate the mechanisms by which ELA promoted the migration of BMSCs. …”
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PLGA-based biocompatible nanoparticles improved anti-breast tumor efficacy of 1,25(OH)2D3 through the SOCS3/STAT3/EMT signaling axis by JinJing Pan, Ping Wang, Linghong Xiong, Wenqing Yang, Jie Li, Kai Yang, Bingyan Li

“…It is noteworthy that PD significantly alleviates the adverse effects of increased serum calcium levels and weight loss in mice induced by 1,25(OH)2D3. Subsequently, bioinformatics analysis and western blot confirmed that PD showed a more pronounced upregulation of SOCS3 and a concurrent downregulation of JAK/STAT3 and PI3K/AKT signals compared to 1,25(OH)2D3 both in vivo and in vitro. …”
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Mechanism study of KCMF1 promoting proliferation and NF-κB signaling transduction in colorectal cancer cells by WU Zhibai, XU Guiqin, ZHANG Li, YANG Zhaojuan, LIU Yun, JIAO Kun, CHEN Zehong, XU Chen, ZUO You, ZHENG Ningqian, YE Zhiqian, LIU Yongzhong

“…Changes in the transcriptional profile in HCT116 cells upon KCMF1 knockdown were identified by RNA sequencing (RNA-Seq), and the affected signaling pathways were evaluated by bioinformatics analysis. Real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR), Western blot, luciferase reporter assay and cell immunofluorescence assay were utilized to validate the alteration of the affected signaling pathway. …”
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Genetic investigations of autosomal recessive inherited ichthyosis impressed by fetal ultrasound: Exome sequencing and haplotype linkage analysis by Ting-Yu Chang, Zhu Wei Lim, Yi-Tzu Chu, Wan-Ju Wu, Mei-Hui Lee, Shun-Ping Chang, Dong-Jay Lee, Wen-Hsiang Lin, Ming Ho, Jin-Chung Shih, Gwo-Chin Ma, Ming Chen

“…Conclusion: Preimplantation genetic testing using STR linkage analysis can rescue cases with autosomal recessive inheritance when only one mutation is found in the putative gene, while reanalysis of high-coverage WES by optimized updated bioinformatics should always be considered in addition to whole genome sequencing.…”
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A gut instinct for childhood leukemia prevention: microbiome-targeting recommendations aimed at parents and caregivers by Ersen Kameri, Ersen Kameri, Ersen Kameri, Vera Helena Jepsen, Pawel Stachura, Pawel Stachura, Nadine Rüchel, Rigveda Bhave, Leticia Benitez, Fatima Crispi, Eduard Gratacos, Nico Dragano, Stefan Janssen, Arndt Borkhardt, Arndt Borkhardt, Aleksandra Pandyra, Aleksandra Pandyra, Aleksandra Pandyra, Gesine Kögler, Gesine Kögler, Ute Fischer, Ute Fischer, Ute Fischer

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Development of an accelerometer age- and sex-specific approach based on population-standardized values for physical activity surveillance: A proof of concept by Jairo H. Migueles, Cristina Cadenas-Sanchez, Nicole M. Butera, David R. Bassett, Dana L. Wolff-Hughes, Jennifer A. Schrack, Pedro F. Saint-Maurice, Eric J. Shiroma

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EDF1 accelerates ganglioside GD3 accumulation to boost CD52-mediated CD8+ T cell dysfunction in neuroblastoma by Di Li, Meng Li, Zhenjian Zhuo, Huiqin Guo, Weixin Zhang, Yile Xu, Hai-Yun Wang, Jiabin Liu, Huimin Xia, Huiran Lin, Jue Tang, Jing He, Lei Miao

“…Methods We performed bulk-RNA sequencing and lipidomic analyses of children with mediastinal NB. Bioinformatics analysis and biological validation were applied to uncover the underlying mechanism. …”
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A meta-analysis of epigenome-wide association studies of ultra-processed food consumption with DNA methylation in European children by Joana Llauradó-Pont, Nikos Stratakis, Giovanni Fiorito, Evangelos Handakas, Alexander Neumann, Henrique Barros, Anne Lise Brantsæter, Kiara Chang, Leda Chatzi, Janine F. Felix, Regina Grazuleviciene, Vincent W. V. Jaddoe, Marianna Karachaliou, Marion Lecorguillé, Carla Lopes, Christopher Millett, Rosemary R. C. McEachan, Eleni Papadopoulou, Remy Slama, Eszter P. Vamos, Paolo Vineis, Martine Vrijheid, John Wright, Trudy Voortman, Mariona Bustamante, Oliver Robinson, Camille Lassale

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