Consistent RNA expression and RNA modification patterns in cardiotoxicity induced by Matrine and Evodiamine by Guanhua Fang, Guanhua Fang, Yanming Shen, Xinyue Gao, Lele Yang, An Zhu, Dongshan Liao, Dongshan Liao

“…In this study, we employed a comprehensive bioinformatics analysis to present sequencing data obtained from AC16 myocardial cells treated with two bioactive derived from botanical drugs: Matrine and Evodiamine. …”
Get full text

Therapeutic Effect of Gypenosides on Antioxidant Stress Injury in Orbital Fibroblasts of Graves’ Orbitopathy by Chao Ma, Haoyu Li, Wei Liu, Shuwen Lu, Xian Li, Jinyuan Chen, Kaijun Li, Wenzhan Wang

“…The relationship between Gyps and GO oxidative stress was understood by bioinformatics analysis. Orbital connective tissues of GO and non-GO patients were obtained for primary OF culture. …”
Get full text

A strategy to increase identification of patients with Familial Hypercholesterolemia: Application of the Simon Broome lipid criteria in a large-scale retrospective analysis by James K. Fleming, Renee M. Sullivan, David Alfego, Natalia T. Leach, Tamara J. Richman, Jill Rafalko

Get full text

Unravelling the TCRβ repertoire: a key to unlocking the immunopathogenesis and precision medicine in SLE by Qiao Zhou, Li Zeng, Zhixin Zhang, Jian Liu, Yichen Zhang, Yang An, Lijing Yang, Tianzuo Lan, Pengming He, Xueping Wen, Shaoping Deng

“…Our study aimed to delineate the variances in the TCRβ repertoire between patients with SLE and healthy individuals, correlating these differences with the severity and subtypes of SLE.Methods We conducted an analysis of blood samples from 50 treatment-naive patients with SLE and 50 healthy donors, employing RNA extraction, high-throughput sequencing and subsequent bioinformatics analysis.Results Our findings revealed significant alterations in TRBV and TRBJ gene usage frequencies, indicative of a skewed TCR repertoire in patients with SLE. …”
Get full text

G-cleave LC3B biosensor: monitoring autophagy and assessing resveratrol's synergistic impact on doxorubicin-induced apoptosis in breast cancer cells by Chiao-Chun Liao, Yuqing Long, Ming-Lin Tsai, Chun-Yu Lin, Kai-Wen Hsu, Chia-Hwa Lee

Get full text

Hypericin mediated photodynamic therapy induces ferroptosis via inhibiting the AKT/mTORC1/GPX4 axis in cholangiocarcinoma by Wei An, Kai Zhang, Guangbing Li, Shunzhen Zheng, Yukun Cao, Jun Liu

“…Then, we conducted transcriptome sequencing and bioinformatics analysis and observed that HY-PDT was most likely involved in ferroptosis, apoptosis, the EMT process and AKT/mTORC1 signaling pathways in cholangiocarcinoma cells. …”
Get full text

Computational biology assisted exploration of phytochemicals derived natural inhibitors to block BZLF1 gene activation of Epstein–Bar virus in host by Muhammad Naveed, Muzamal Hussain, Tariq Aziz, Nimra Hanif, Nazia Kanwal, Arooj Arshad, Ayaz Ali Khan, Abdulrahman Alshammari, Metab Alharbi

“…The current study performed computer-assisted techniques to analyses the potential drug candidates against Epstein–Barr virus toxin Zta [Human gamma-herpesvirus 4 (Epstein–Barr virus)] from phytochemicals derived natural inhibitors. Various bioinformatics methods were employed to predict and analyze the toxin protein structure obtained from NCBI, and its secondary and tertiary structures were predicted using PSIPRED and the AlphaFold. …”
Get full text

Rectal mucosal inflammation, microbiome, and wound healing in men who have sex with men who engage in receptive anal intercourse by Vanessa E. Van Doren, Cassie G. Ackerley, Robert A. Arthur, Phillip M. Murray, S. Abigail Smith, Yi-Juan Hu, Colleen F. Kelley

Get full text

ADAMDEC1 promotes the malignant progression of cholangiocarcinoma by regulating NF-κB signaling pathway by Shuo Zhou, Yuhang Yang, Feiyu Qi, Wanliang Sun, Dengyong Zhang, Zheng Lu, Yongliang Chen

“…Meanwhile, the interacting proteins of ADAMDEC1 and its involvement in the nuclear factor-kappa B (NF-κB) signaling pathway were screened and verified through bioinformatics analysis. The tumorigenicity of CCA was also assessed in a xenograft nude mouse model. …”
Get full text

Vaginal Microbiota Diversity of Patients with Embryonic Miscarriage by Using 16S rDNA High-Throughput Sequencing by Linfen Xu, Lingna Huang, Chengying Lian, Huili Xue, Yanfang Lu, Xiujuan Chen, Yong Xia

“…To further dissect the microbiota of the vagina in the two groups, 16S rDNA sequencing was performed. Bioinformatics analysis showed that 1096 and 998 overlapped operational taxonomic units were identified from the embryonic miscarriage and control groups, respectively. …”
Get full text

Engineering of Dihydroxy Biphenyl Dioxygenase Enzyme to Improve Its Performance In Removing PCBs: A Molecular Docking Study by Susan Yazdankhah, Alireza Zakeri, Saeed Khalili, Abolfazl Jahangiri, ZahraalSadat Hashemi, Alireza Heidari

“…This research has been carried out in bioinformatics to strengthen the enzyme and weaken the inhibitory effect through mutation in the amino acids of the active site. …”
Get full text

A universal DNA microarray for rapid fish species authentication by Patrizia Bade, Sebastian Stix, Kristina Kappel, Jan Fritsche, Ilka Haase, Andrew Torda, Nils Wax, Markus Fischer, Dirk Brandis, Ute Schröder

Get full text

Resetting the Hsc70-mediated lysosomal degradation of PD-L1 via a supramolecular meso peptide for the restoration of acquired anti-tumor T cell immunity by Yujia Zhao, Dan Liu, Wenguang Yang, Wangxiao He, Jin Yan, Leiqing Yao

“…Furthermore, based on bioinformatics analysis of mRNA expression data from 30 types of tumors obtained from TCGA database, malignant melanoma was identified as the most suitable indication for CTAC-PDL1 due to its specific characteristics of tumor immune. …”
Get full text

Efficiency to Discovery Transgenic Loci in GM Rice Using Next Generation Sequencing Whole Genome Re-sequencing by Doori Park, Dongin Kim, Green Jang, Jongsung Lim, Yun-Ji Shin, Jina Kim, Mi-Seong Seo, Su-Hyun Park, Ju-Kon Kim, Tae-Ho Kwon, Ik-Young Choi

“…Results of this study demonstrated the success of NGS data to characterize the rice genome. Bioinformatics analyses must be developed in association with NGS data to identify highly accurate transgenic sites.…”
Get full text

Crotonylation-Related Prognostic Model of Esophageal Squamous Cell Carcinoma Based on Transcriptome Analysis and Single-Cell Sequencing Analysis by Lin RY, Chen RP, Lin FQ

“…This study aimed to investigate the role of crotonylation-related genes (CRGs) in ESCC using bioinformatics approaches.Methods: We included three ESCC datasets and 24 CRGs. …”
Get full text

Lc-ms-based untargeted metabolomics reveals potential mechanisms of histologic chronic inflammation promoting prostate hyperplasia. by Jiale Li, Beiwen Wu, Guorui Fan, Jie Huang, Zhiguo Li, Fenghong Cao

“…Selected differential endogenous metabolites were analyzed using bioinformatics and subjected to metabolic pathway studies.…”
Get full text

Differential Regulation of NK Cell Receptors in Acute Lymphoblastic Leukemia by Le Jie Lee, Norfarazieda Hassan, Siti Zuleha Idris, Suresh Kumar Subbiah, Heng Fong Seow, Norhafizah Mohtaruddin, Kian Meng Chang, Raudhawati Osman, Hishamshah Mohd Ibrahim, Sheila Nathan, Maha Abdullah

“…Quantitative PCR (q-PCR) further investigated copy number variation (CNV), methylation index (MI), and mRNA expression of significant genes in T-ALL (14 males), NC (12 males and 12 females), and B-ALL samples (N=12 males and 12 females). Bioinformatics revealed unique variants particularly rs2253849 (T>C) in KLRC1 and rs1141715 (A>G) in KLRC2 only among T-ALL (allele frequency 0.8-1.0). …”
Get full text

LncRNA GAS5 reduces blood glucose levels and alleviates renal fibrosis in diabetic nephropathy by regulating the miR-542-3p/ERBB4 axis by Qinghua Yin, Na Guo, Ruoxi Liao

“…ELISA was executed to examine the levels of IL-1β, IL-6, and TNF-α; and the superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) activities were evaluated. Bioinformatics analysis, dual-luciferase and RIP assays were performed to verify the relationship between lncRNA GAS5 and miR-542-3p, and miR-542-3p and ERBB4. …”
Get full text

Identification of the Key Genes and Pathways in Esophageal Carcinoma by Peng Su, Shiwang Wen, Yuefeng Zhang, Yong Li, Yanzhao Xu, Yonggang Zhu, Huilai Lv, Fan Zhang, Mingbo Wang, Ziqiang Tian

“…Differentially expressed genes (DEGs) were screened by bioinformatics analysis. Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and protein-protein interaction (PPI) network construction were performed to obtain the biological roles of DEGs in EC. …”
Get full text

Deregulation mechanisms and therapeutic opportunities of p53-responsive microRNAs in diffuse large B-cell lymphoma by Elena N. Voropaeva, Yuriy L. Orlov, Anastasia B. Loginova, Olga B. Seregina, Vladimir N. Maksimov, Tatiana I. Pospelova

“…Development of new bioinformatics tools and databases for microRNA supports DLBCL research. …”
Get full text