Toll-like receptor 4 pathway evolutionary trajectory and functional emergence by Shailya Verma, Ramanathan Sowdhamini, Ramanathan Sowdhamini, Ramanathan Sowdhamini

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Multi-omics analysis reveals that neutrophil extracellular traps related gene TIMP1 promotes CRC progression and influences ferroptosis by Yuzhao Jin, Luyu Liao, Qianping Chen, Bufu Tang, Jin Jiang, Ji Zhu, Minghua Bai

“…Biological experiments have proven that TIMP1 can promote the proliferation, invasion and migration of CRC. Conclude Bioinformatics analysis combined with experimental verification showed that TIMP1 is related to ferroptosis and plays a promoting role in the invasion, migration and proliferation of CRC.…”
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Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli by Jie Fang, Xiaojiao Han, Lihua Xie, Mingying Liu, Guirong Qiao, Jing Jiang, Renying Zhuo

“…Here, a full-length cDNA library of A. glaucus under salt stress was constructed to identify genes related to salt tolerance, and one hundred clones were randomly selected for sequencing and bioinformatics analysis. Among these, 82 putative sequences were functionally annotated as being involved in signal transduction, osmolyte synthesis and transport, or regulation of transcription. …”
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Palmitoylation-related gene ZDHHC22 as a potential diagnostic and immunomodulatory target in Alzheimer’s disease: insights from machine learning analyses and WGCNA by Sanying Mao, Xiyao Zhao, Lei Wang, Yilong Man, Kaiyuan Li

“…Conclusions This study is the first to use bioinformatics methods to identify ZDHHC22 as a key KPRG in AD, highlighting its potential role in disease diagnosis and immune regulation. …”
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A Protein with Unknown Function, Ps495620, Is Critical for the Sporulation and Oospore Production of <i>Phytophthora sojae</i> by Xiaoran Du, Yan Zeng, Yiying Li, Qin Peng, Jianqiang Miao, Xili Liu

“…While the rapid rise in bioinformatics has facilitated the identification of the domains and functions of many proteins, some still have no domain annotation or largely uncharacterized functions. …”
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Electroacupuncture-Regulated miR-34a-3p/PDCD6 Axis Promotes Post-Spinal Cord Injury Recovery in Both In Vitro and In Vivo Settings by Lili Ma, Lizhong Ma, Yu Yang, Ting Chen, Limin Wang, Qilong Deng

“…In our study, based on bioinformatics prediction, we found that miR-34a-3p might be an upstream regulator miRNA for PDCD6, which was subsequently validated through combined utilization of the qRT-PCR, western blot, and dual-luciferase reporter system. …”
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Stathmin 1 expression in neuroendocrine and proliferating prostate cancer by Yingli Shi, Yunshin A. Yeh, Siyuan Cheng, Xin Gu, Shu Yang, Lin Li, Nazih P. Khater, Susan Kasper, Xiuping Yu

“…In this study, we investigated the expression of stathmin 1 (STMN1) across PCa subtypes using bioinformatics, western blotting, and immunohistochemical staining analyses in human and murine models. …”
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Identifying the NEAT1/miR-26b-5p/S100A2 axis as a regulator in Parkinson's disease based on the ferroptosis-related genes. by Taole Li, Jifeng Guo

“…<h4>Methods</h4>Expression of 97 substantia nigra (SN) samples were obtained and intersected with FRGs. Bioinformatics analysis, including the gene set enrichment analysis (GSEA), consensus cluster analysis, weight gene co-expression network analysis (WGCNA), and machine learning algorithms, were employed to assess the feasible differentially expressed genes (DEGs). …”
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Exploration and Verification of Prognostic Value of Endothelial Cells in Glioblastoma by Hengchao MA, Yuyang LIU, Jun XU, Bingyan TAO, Jun ZHANG

“…ObjectiveTo explore and verify the prognostic value of endothelial cells in glioblastoma. MethodsThrough bioinformatics analysis of the TCGA and CGGA databases, we screened endothelial cell-related markers in GBM single-cell data according to a series of criteria. …”
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Rosuvastatin effects on the HDL proteome in hyperlipidemic patients by Vavlukis Ana, Mladenovska Kristina, Davalieva Katarina, Vavlukis Marija, Dimovski Aleksandar

“…A label-free LC-MS/MS protein profiling was conducted, coupled with bioinformatics analysis. Sixty-nine HDL proteins were identified, belonging to four main biological function clusters: lipid transport and metabolism; platelet activation, degranulation, and aggregation, wound response and wound healing; immune response; inflammatory and acute phase response. …”
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Acid sphingomyelinase modulates anxiety-like behavior likely through toll-like receptor signaling pathway by Huiqi Yuan, Yanan Xu, Hailun Jiang, Meizhu Jiang, Luofei Zhang, Shifeng Wei, Cao Li, Zhigang Zhao

“…Transcriptional profiling combined with bioinformatics analysis revealed the upregulation of Toll-like receptor signaling pathway related gene including Tlr1/2, Ccl3, Ccl4, Ccl5 and Cd86 in Asm KO mice, which was further confirmed by the detection of activated microglia and astrocytes through iba-1 and GFAP immunohistochemical staining. …”
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The Relationship between Heart Rate Variability and Adiposity Differs for Central and Overall Adiposity by B. Gwen Windham, Stefano Fumagalli, Alessandro Ble, John J. Sollers, Julian F. Thayer, Samar S. Najjar, Michael E. Griswold, Luigi Ferrucci

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Identifying novel heterozygous PI4KA variants in fetal abnormalities by Chen Cheng, Fan Yang, Xinlin Chen, Sheng Zhao

“…The functional consequences of these variants were evaluated using bioinformatics tools. The effects of the identified variants on splicing were analyzed through minigene splicing assays. …”
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Detection of gene clusters for biodegradation of alkanes and aromatic compounds in the Rhodococcus qingshengii VKM Ac-2784D genome by Yu. A. Markova, I. S. Petrushin, L. A. Belovezhets

“…The R. qingshengii VKM Ac-2784D genome contains the otsA, otsB, treY, treZ genes. The bioinformatics data are supported by the previous biochemical experiments that allow a mixture of species with a wide variation of metabolic pathways to be obtained.…”
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Immunoinformatics design of a novel multiepitope vaccine candidate against non-typhoidal salmonellosis caused by Salmonella Kentucky using outer membrane proteins A, C, and F. by Elayoni E Igomu, Paul H Mamman, Jibril Adamu, Maryam Muhammad, Abubarkar O Woziri, Manasa Y Sugun, John A Benshak, Kingsley C Anyika, Rhoda Sam-Gyang, David O Ehizibolo

“…In this study, we used a bioinformatics approach to develop a peptide-based vaccine targeting epitopes from the outer membrane proteins A, C, and F of S. …”
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Multidisciplinary approach to functional somatic syndromes: study protocol for a population-based prospective cohort study by Hamidreza Roohafza, Harold Snieder, Fatemeh Hadizadeh, Peyman Adibi, Alireza Ani, Ahmad Vaez

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Determining correct tracheal tube insertion depth by measuring distance between endotracheal tube cuff and vocal cords by ultrasound in Chinese adults: a prospective case-control s... by Xuanling Chen, Wenwen Zhai, Zhuoying Yu, Jiao Geng, Min Li

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The up-regulation of SPTAN1 expression in Pancreatic adenocarcinoma is associated with tumor immune invasion and poor clinical prognosis by Wei Guo, LingYu Hu, ZhaoFeng Gao, XiaoRong Liu, XiaoDan Yang, XiaoGuang Wang

“…., and may affect their prognosis. Methods Bioinformatics technology was used to analyze the relationship between SPTAN1 expression in PAAD and immune cell infiltration, immune regulatory factors and chemokines, and cell experiments were used to verify the relationship between SPTAN1 knock down and migration, invasion, apoptosis and cycle changes of PAAD cell lines. …”
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Analysis methods for covariate-constrained cluster randomized trials with time-to-event outcomes by Amy M. Crisp, M. Elizabeth Halloran, Matt D. T. Hitchings, Ira M. Longini, Natalie E. Dean

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Hairpin in a haystack: In silico identification and characterization of plant-conserved microRNA in Rafflesiaceae by Wicaksono Adhityo, Meitha Karlia, Wan Kiew-Lian, Mat Isa Mohd Noor, Parikesit Arli Aditya, Molina Jeanmaire

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