Broad substrate scope C-C oxidation in cyclodipeptides catalysed by a flavin-dependent filament by Emmajay Sutherland, Christopher J. Harding, Tancrède du Monceau de Bergendal, Gordon J. Florence, Katrin Ackermann, Bela E. Bode, Silvia Synowsky, Ramasubramanian Sundaramoorthy, Clarissa Melo Czekster

“…Our work elucidates the complex mechanistic and structural characteristics of this dehydrogenation reaction, positioning NdasCDO as a promising biocatalyst and expanding the FMN-dependent oxidase family to include enzyme filaments.…”
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Improving the power production efficiency of microbial fuel cell by using biosynthesized polyanaline coated Fe3O4 as pencil graphite anode modifier by Tekalign Tesfaye, Yohannes Shuka, Sisay Tadesse, Tesfahun Eyoel, Mesele Mengesha

“…Abstract A microbial fuel cell (MFC) is a modern, environmentally friendly, and cost-effective energy conversion technology that utilizes renewable organic waste as fuel, converting stored chemical energy into usable bioelectricity in the presence of a biocatalyst. Despite advancements in MFC technology, several challenges remain in optimizing power production efficiency, particularly regarding anode materials and modifications. …”
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Extracellular Lipase from Pseudomonas aeruginosa SB-37: Production by Solid State Fermentation, Immobilization, and Characterization by Titin Haryati, Norman Yoshi Haryono, Dian Nugraheni, Fenti Fatmawati, Alfina Kusuma Dewi, Muhammad Zahran Edlian Syach

“…Native lipase is still promising as an industrial biocatalyst. This study aimed to investigate the production of native local lipase using solid state fermentation (SSF) methods, immobilization the lipase by Ca-alginate entrapment, and characterization based on substrate preferences. …”
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Microbial Fuel Cell: Recent Developments in Organic Substrate Use and Bacterial Electrode Interaction by Fatin Syahirah Fadzli, Showkat Ahmad Bhawani, Rania Edrees Adam Mohammad

“…A new bioelectrochemical approach based on metabolic activities inoculated bacteria, and the microbial fuel cell (MFC) acts as biocatalysts for the natural conversion to energy of organic substrates. …”
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Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in <i>Pichia pastoris</i> by Shuyan Wu, David Hooks, Gale Brightwell

“…The key factors influencing the heterologous expression of polymer-degrading enzymes in <i>P. pastoris</i> are discussed, shedding light on the challenges and opportunities in the development of depolymerising biocatalysts through the <i>P. pastoris</i> expression system.…”
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Coordinated conformational changes in P450 decarboxylases enable hydrocarbons production from renewable feedstocks by Wesley Cardoso Generoso, Alana Helen Santana Alvarenga, Isabelle Taira Simões, Renan Yuji Miyamoto, Ricardo Rodrigues de Melo, Ederson Paulo Xavier Guilherme, Fernanda Mandelli, Clelton Aparecido Santos, Rafaela Prata, Camila Ramos dos Santos, Felippe Mariano Colombari, Mariana Abrahão Bueno Morais, Rodrigo Pimentel Fernandes, Gabriela Felix Persinoti, Mario Tyago Murakami, Leticia Maria Zanphorlin

“…Abstract Fatty acid peroxygenases have emerged as promising biocatalysts for hydrocarbon biosynthesis due to their ability to perform C-C scission, producing olefins - key building blocks for sustainable materials and fuels. …”
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Organic Solvent Tolerant Lipases and Applications by Shivika Sharma, Shamsher S. Kanwar

“…Also, the potential industrial applications that make lipases the biocatalysts of choice for the present and future have been presented.…”
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Accelerated enzyme engineering by machine-learning guided cell-free expression by Grant M. Landwehr, Jonathan W. Bogart, Carol Magalhaes, Eric G. Hammarlund, Ashty S. Karim, Michael C. Jewett

“…Our ML-guided, cell-free framework promises to accelerate enzyme engineering by enabling iterative exploration of protein sequence space to build specialized biocatalysts in parallel.…”
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Glycerol Carbonate: A Novel Biosolvent with Strong Ionizing and Dissociating Powers by Guangnan Ou, Biyan He, Xuejing Li, Jianhui Lei

“…The activity of biocatalysts in nonaqueous solvents is related to the interaction of organic solvents with cells or enzymes. …”
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Mining versatile feruloyl esterases: phylogenetic classification, structural features, and deep learning model by Liang Guo, Yuxin Dong, Deyong Zhang, Xinrong Pan, Xinjie Jin, Xinyu Yan, Yin Lu

“…Our findings underscore the utility of integrating phylogenetic and structural features with deep learning approaches for mining versatile FEs, shedding light on unexplored enzymatic diversity and expanding the repertoire of biocatalysts for synthetic applications. Graphical Abstract…”
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Fine tuning enzyme activity assays for monitoring the enzymatic hydrolysis of PET by Krisztina Boros, Blanka Eszter Nagy, Raluca Bianca Tomoiagă, Róbert Tőtős, Monica Ioana Toșa, Csaba Paizs, László Csaba Bencze

“…Abstract Efficient monitoring of the enzymatic PET-hydrolysis is crucial for developing novel plastic-degrading biocatalysts. Herein, we aimed to upgrade in terms of accuracy the analytical methods useful for monitoring enzymatic PET-degradation. …”
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Exploring the capacity of microorganism treatment for fermentation and glycosidic aroma bioconversion from rose oil distillation wastewater by Mohammad Karami, Mohsen Bagheri, Alireza Abbasi-Baharanchi, Kamahladin Haghbeen, Abdolrahim Nouri, Ali Asghar Karkhane, Mansour Ghorbanpour, Mohsen Farhadpour

“…Conclusion Using different microbial biocatalysts to recover natural bioactive aroma compounds is an attractive and scalable bioconversion methodology for producing value-added chemical derivatives from the waste of medicinal plants. …”
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Response of alcohol fermentation strains, mixed fermentation and extremozymes interactions on wine flavor by Ling-Zhi Zhao, Ling-Zhi Zhao, Jing Chen, Jing Chen, Jing Chen, Xiang-Ying Wei, Xiang-Ying Wei, Xiang-Ying Wei, Bo Lin, Feng-Jin Zheng, Krishan K. Verma, Gan-Lin Chen, Gan-Lin Chen, Gan-Lin Chen

“…Extremophilic microorganisms have established different molecular strategies to survive amidst the adverse conditions. Biocatalysts isolated by these organisms are termed extremozymes and possess extraordinary properties of salt allowance, thermostability, and cold adaptability. …”
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Enrichment of electrotrophic microorganisms from contrasting shallow-sea hydrothermal environments in bioelectrochemical reactors by Antoine Carissimo, Victoria Comes, Alenica Heussner, Anne-Hélène Prime, Roy E. Price, Gaël Erauso, Pierre-Pol Liebgott, Sven Kerzenmacher, Guillaume Pillot

“…In reactors reproducing the Prony Bay hydrothermal conditions, known facultative methylotrophs, such as Sphingomonas and Methylobacterium, were dominant and appeared to consume formate (provided as carbon source) but no electrons from the cathode.ConclusionThese results provide new insights into the distribution and diversity of electrotrophs in shallow-sea hydrothermal vents and allow the identification of potential novel biocatalysts for Microbial Electrosynthesis whereby electricity and carbon dioxide are converted into value-added products.…”
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