Single-cell profiling transcriptomic reveals cellular heterogeneity and cellular crosstalk in breast cancer lymphatic node, bone, and brain metastases by Longyu Zhu, Miaomiao Liu, Yuguang Shang, Jingge Cheng, Hongye Zhao, Jun Zhang, Dongxing Shen

“…We identified five T cells (T memory, CD8 + T cells, regulatory T cells, natural killer cells, CD4 + T cells), three B cells (naïve B cells, memory B cells, plasma B cells), and five cancer-associated fibroblasts (CAFs) subpopulations (Smooth muscle cells (SMC), pericyte, antigen-presenting CAFs (apCAFs), proliferative CAFs (pCAFs), and matrix CAFs (mCAFs)). …”
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Integrating traditional biomarkers and emerging predictors to assess neoadjuvant chemotherapy efficacy in breast cancer: a multifactorial analysis of Ki-67, CDK4, EGFR, TILs and ct... by Tianzhao Du, Ye Yuan, Shulan Sun, Zhichao Gao, Xiaoshuai Li

“…Abstract Objective This study aimed to analyse the correlation between the expression of cell proliferation-associated antigen (Ki-67), cell cycle protein-dependent kinase 4 (CDK4), epidermal growth factor receptor (EGFR), tumour-infiltrating lymphocytes (TILs) and circulating tumour DNA (ctDNA) with the outcome and prognosis of patients with breast cancer (BC) undergoing neoadjuvant chemotherapy (NACT). …”
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Characterization of <i>Streptococcus pyogenes</i> Strains from Tonsillopharyngitis and Scarlet Fever Resurgence, 2023—FIRST Detection of M1_UK in Bulgaria by Emma Keuleyan, Theodor Todorov, Deyan Donchev, Ani Kevorkyan, Radoslava Vazharova, Alexander Kukov, Georgi Todorov, Boriana Georgieva, Iskra Altankova, Yordanka Uzunova

“…In our study, the infections were confirmed by culture testing or rapid antigen test. Identification was performed by MALDI-TOF and was followed up by antibiotic susceptibility testing (EUCAST). …”
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Assessment of Physicochemical Properties of Rituximab Related to Its Immunomodulatory Activity by Mariana P. Miranda-Hernández, Carlos A. López-Morales, Nancy D. Ramírez-Ibáñez, Nelly Piña-Lara, Nestor O. Pérez, Aarón Molina-Pérez, Jorge Revilla-Beltri, Luis F. Flores-Ortiz, Emilio Medina-Rivero

“…It binds specifically to the CD20 antigen expressed on pre-B and consequently on mature B-lymphocytes of both normal and malignant cells, inhibiting their proliferation through apoptosis, CDC, and ADCC mechanisms. …”
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Discovery of a novel highly specific, fully human PSCA antibody and its application as an antibody-drug conjugate in prostate cancer by Xiaojie Chu, Seungmin Shin, Du-San Baek, Liyong Zhang, Alex Conard, Megan Shi, Ye-Jin Kim, Cynthia Adams, Maggie Hines, Xianglei Liu, Chuan Chen, Zehua Sun, Dontcho V. Jelev, John W. Mellors, Dimiter S. Dimitrov, Wei Li

“…Prostate stem cell antigen (PSCA) is expressed in all stages of prostate cancer, including in advanced androgen-independent tumors and bone metastasis. …”
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Case report: PD-L1-targeted high-affinity natural killer cells and IL-15 superagonist N-803-based therapy extend overall survival of advanced metastatic pancreatic cancer patients by Tara Seery, Lennie Sender, Omid Jafari, Frank Jones, Patricia Spilman, Sandeep B. Reddy, Patrick Soon-Shiong

“…Duration of spIND treatment and responses, for some patients including imaging and carbohydrate antigen 19-9 (CA19-9) levels, and OS from initial diagnosis and the start of spIND therapy were assessed.FindingsThe line/duration of spIND therapy was, for patients 1 through 5, respectively, second line/6.4 months, sixth line/3.5 months, third line/25.4 months, third line/7.4 months, and fourth line/23.2 months. …”
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Blood absolute lymphocyte count and trajectory are important in understanding severe COVID-19 by Catharine I. Paules, Jacqueline A. Nordwall, Kathryn Shaw-Saliba, Judith A. Aberg, Edward M. Gardner, Anna L. Goodman, N. Kumarasamy, Shikha Vasudeva, David M. Vock, Crystal M. North, Jens Lundgren, Neil R. Aggarwal, for the STRIVE Network and TICO Trial Study Group

“…Persistent and new lymphopenia were associated with older age, male sex; prior immunosuppression, heart failure, aspirin use, and normal body mass index; biomarkers of organ damage (renal and lung), and ineffective immune response (elevated IL-6 and viral nucleocapsid antigen levels). Similar results were observed with severe lymphopenia. …”
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Immunogenicity and safety of HepE Hecolin® in chronic hepatitis B patients at clinically stable stage: An open-label study in China by Li Zhang, Qiufen Zhang, Jiaye Liu, Wenlong Wu, Zechun Jiang, Bingyu Yan, Qingfan Cao, Haidong Liu, Huirong Pan, Jingjing Lv, Yi Feng, Fujie Xu, Shoujie Huang, Aiqiang Xu

“…The control group included healthy adults who had hepatitis B surface antigen (HBsAg) negative. HepE was administered for 0, 1, 6 months for all participants. …”
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Lipid nanoparticles deliver DNA-encoded biologics and induce potent protective immunity by Dafei Chai, Junhao Wang, Jing Ming Lim, Xiaohui Xie, Xinfang Yu, Dan Zhao, Perry Ayn Mayson Maza, Yifei Wang, Dana Cyril-Remirez, Ken H. Young, Yong Li

“…Notably, a DNA vaccine encoding the spike protein, delivered via LNP-M, induced stronger antigen-specific antibody and T cell immune responses compared to electroporation. …”
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Identification and Functional Analysis of PANoptosis‐Associated Genes in the Progression From Sepsis to ARDS by Zhong‐Hua Lu, Yan Tang, Hu Chen, Feng Liu, Mei Liu, Lu Fu, Xian‐Kai Wang, Ming‐Juan Li, Wei‐Li Yu, Yun Sun

“…Functional enrichment analysis highlighted spliceosome function, MAPK signaling, endocytosis, and antigen processing pathways as significantly associated with these PRGs. …”
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Antidepressant Effects of Probucol on Early-Symptomatic YAC128 Transgenic Mice for Huntington’s Disease by Cristine de Paula Nascimento-Castro, Ana Claudia Wink, Victor Silva da Fônseca, Claudia Daniele Bianco, Elisa C. Winkelmann-Duarte, Marcelo Farina, Ana Lúcia S. Rodrigues, Joana Gil-Mohapel, Andreza Fabro de Bem, Patricia S. Brocardo

“…Endogenous markers were used to assess the effect of probucol on cell proliferation (Ki-67 and proliferation cell nuclear antigen (PCNA)) and neuronal differentiation (doublecortin (DCX)) in the hippocampal dentate gyrus (DG). …”
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Diagnosis and Treatment of Peptic Ulcer in Adults (Clinical Guidelines of the Russian Gastroenterological Association, Russian Society of Colorectal Surgeons and the Russian Endosc... by V. T. Ivashkin, I. V. Maev, P. V. Tsar’kov, M. P. Korolev, D. N. Andreev, E. K. Baranskaya, S. G. Burkov, A. A. Derinov, S. K. Efetov, T. L. Lapina, P. V. Pavlov, S. S. Pirogov, A. V. Tkachev, A. S. Trukhmanov, E. D. Fedorov, A. A. Sheptulin

“…In order to determine indications for eradication therapy, all ulcer patients should be tested for the presence of H. pylori using a 13C-breath test or a stool antigen test. In the case of simultaneous endoscopy, rapid urease test can be used. …”
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The Relationship of Tumor-infiltrating Lymphocyte Ratio with Histopathological Parameters and Effect on Survival in Colorectal Cancers by İsra Serda Oğuz, Sinan Koca, Seval Ay Ersoy, Özgecan Dülgar, Ayşenur Toksöz, Mahmut Gümüş

“…Clinical characteristics, age, gender, histopathologic features, TIL ratio, and carcinoembryonic antigen (CEA) level of the patients were recorded. …”
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Optimization of temperature parameters for screening unexpected antibodies in Rh system by manual polybrene test by ZOU Xin, CHEN Minjie, MA Sifei, YANG Hongmei

“…[Methods] The reaction of IgG human anti-D antibody with different dilutions (undiluted, 1∶2, 1∶4, 1∶8, 1∶16, 1∶32，1∶64) with D antigen-positive red blood cells was detected by manual polybrene test (MPT). …”
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CD93 blockade promotes effector T-cell infiltration and facilitates adoptive cell therapy in solid tumors by Yi Sun, Eduardo Davila, YuJie Guo, Richard D Schulick, Elliott Yee, Yuki Fujiwara, Kaitlyn Dickinson, Zhiwei Sun, Junyi Hu, Yuwen Zhu

“…Background Adaptive cellular therapy (ACT), particularly chimeric antigen receptor (CAR)-T cell therapy, has been successful in the treatment of hemopoietic malignancies. …”
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Systematic screening and dynamic profiling of germline regulatory pathways and spermatogonial surface markers in a bivalve mollusc by Liangjie Liu, Ya Shu, Tian Liu, Huilan Wei, Yaxin Yang, Lijing Zhang, Xiaohui Ma, Guoqing Li, Yajuan Li, Shi Wang, Lingling Zhang

“…To obtain antibodies that recognize the cell surface region, the extracellular domains of PyFGFR were used as an antigen to prepare antiserum. Western blotting, immunohistochemical, and immunofluorescent analyses demonstrated that the antiserum specifically detected PyFGFR in the spermatogonia. …”
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Pre-transplant T-cell clonal analysis identifies CD8+ donor reactive clones that contribute to kidney transplant rejection by Jes M. Sanders, Barbara L. Banbury, Erika L. Schumacher, Jie He, Yuvaraj Sambandam, Paul A. Fields, Lorenzo Gallon, Lorenzo Gallon, James M. Mathew, James M. Mathew, James M. Mathew, Joseph R. Leventhal, Joseph R. Leventhal

“…IntroductionResponses to allogeneic human leukocyte antigen (HLA) molecules limit the survival of transplanted organs. …”
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Germinal Center B Cells are Uniquely Targeted by Antibody-Suppressor CXCR5+CD8+ T Cells by Jason M. Zimmerer, PhD, Sachi Chaudhari, BS, Kavya Koneru, BS, Jing L. Han, MD, PhD, Mahmoud Abdel-Rasoul, MA, Hope Uwase, MA, Tai Yi, MD, Christopher K. Breuer, MD, Ginny L. Bumgardner, MD, PhD

“…Adoptive transfer of CD8+ TAb-supp cells into hepatocyte or kidney transplant recipients reduced alloantibody production and the quantity of GC B cells, TFH cells, and plasma cells (but not other B-cell, T-cell, or antigen-presenting cell subsets). The reduction of TFH-cell quantity was dependent on CD8+ TAb-supp cell–mediated major histocompatibility complex-I-dependent cytotoxic killing of GC B cells. …”
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WT1 Peptide Cancer Vaccine for Patients with Hematopoietic Malignancies and Solid Cancers by Yoshihiro Oka, Akihiro Tsuboi, Olga A. Elisseeva, Hiroko Nakajima, Fumihiro Fujiki, Manabu Kawakami, Toshiaki Shirakata, Sumiyuki Nishida, Naoki Hosen, Yusuke Oji, Ichiro Kawase, Haruo Sugiyama

“…The power of a tumor-associated-antigen (TAA)-derived cancer vaccine may be enhanced in combination with stronger adjuvants, helper peptide, molecular-target-based drugs, or some chemotherapy drugs, such as gemcitabine, which has been revealed to suppress regulartory T-cell function. …”
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Single-cell transcriptome atlas of peripheral immune features to Omicron breakthrough infection under booster vaccination strategies by Yuwei Zhang, Shanshan Han, Qingshuai Sun, Tao Liu, Zixuan Wen, Mingxiao Yao, Shu Zhang, Qing Duan, Xiaomei Zhang, Bo Pang, Zengqiang Kou, Xiaolin Jiang, Xiaolin Jiang, Xiaolin Jiang

“…In addition, myeloid cells in the BA.5.2 breakthrough infection with the fourth dose of aerosolized Ad5-nCoV were characterized by enhanced proliferation, chemotactic migration, and antigen presentation.DiscussionCollectively, our study informs the comprehensive understandings of immune characterization for Omicron breakthrough infection, revealing the positive antiviral potential induced by booster doses of vaccine and the possible "trained immunity" phenomenon in the fourth dose of aerosolized Ad5-nCoV, providing a basis for the selection of vaccination strategies.…”
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