MeHg Developing Exposure Causes DNA Double-Strand Breaks and Elicits Cell Cycle Arrest in Spinal Cord Cells by Fabiana F. Ferreira, Dib Ammar, Gilian F. Bourckhardt, Karoline Kobus-Bianchini, Yara M. R. Müller, Evelise M. Nazari

“…Also, to identify neuronal cells, we used anti-NeuN and anti-βIII-tubulin antibodies. After the MeHg treatment, we observed the increase on γ-H2A.X in response to DNA damage. …”
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Multimodality Image Analysis in a Cohort of Patients with Atypical Juvenile Ocular Toxocariasis by Xiaohong Guo, Hui Liu, Manli Li, Ke Fan, Shuyin Li, Bo Lei

“…B-mode ultrasound showed proliferative membranes and proliferative bands (33.3%), as well as spotted opacity in the vitreous (66.7%). The antibodies to Toxocara canis in the aqueous humor and serum were positive, and the Goldmann–Witmer coefficient was significantly increased in 6 out of 7 patients. …”
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Aspiration of acidified milk induces milk allergy by activating alveolar macrophages in mice by Akiko Nakaoka, Takayasu Nomura, Atsushi Suzuki, Kazuyoshi Ozeki, Hirohito Kita, Shinji Saitoh

“…Results: Mice exposed to CM mixed with hydrochloric acid via airway sensitization developed CMA, as evidenced by the production of antigen-specific IgE and IgG antibodies, and the induction of anaphylaxis upon systemic antigen administration. …”
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Anaerobic metabolism promotes breast cancer survival via Histone-3 Lysine-18 lactylation mediating PPARD axis by Ying Xu, Weiwei Meng, Yingqi Dai, Lin Xu, Ning Ding, Jinqing Zhang, Xuewei Zhuang

“…We utilized chromatin immunoprecipitation sequencing with H3K18la antibodies, transcriptomics of clinical BC samples, and proteomics and ATAC-seq analyses of in vivo tumors to identify the genes regulated by H3K18la and the transcription factor PPARD. qPCR and Western blot assays were used to detect expressions of molecules. …”
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Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect by Aniek S. Budiatin, Maria A. Gani, Samirah, Chrismawan Ardianto, Aulia M. Raharjanti, Indah Septiani, Ni Putu K. P. Putri, Junaidi Khotib

“…Radiological examination was performed using haematoxylin and eosin (HE) staining and immunohistochemical techniques with anti-vascular endothelial growth factor (VEGF) and anti-alkaline phosphatase (ALP) antibodies. Based on the radiograph, the implanted group had accelerated bone growth in the defect area. …”
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Immunohistochemical Profile of p62/SQSTM1/Sequestosome-1 in Human Low- and High-Grade Intracranial Meningiomas by Antonio Ieni, Cristina Pizzimenti, Vincenzo Fiorentino, Mariausilia Franchina, Antonino Germanò, Giovanni Raffa, Maurizio Martini, Guido Fadda, Giovanni Tuccari

“…However, intranuclear vacuoles have been ultrastructurally observed in meningiomas, and they were labelled by p62 antibodies. Therefore, in the present study, we have investigated p62 immunohistochemical pattern in a cohort of 133 cases representative of low- and high-grade meningiomas, to verify if p62 expression may be related to clinicopathological data, thus achieving a potential prognostic role. …”
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Alternative buffer systems in biopharmaceutical formulations and their effect on protein stability by Lebar Blaž, Zidar Mitja, Mravljak Janez, Šink Roman, Žula Aleš, Pajk Stane

“…However, conventional buffers like histidine and phosphate buffers may not always be the optimal choice for all monoclonal antibodies (mAbs). In this study, we investigated the effects of several alternative buffer systems on seven different mAbs, exploring various combinations of ionic strengths, concentrations of the main buffer component, mAb concentrations, and stress conditions. …”
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Kaposi's sarcoma-associated herpesvirus (KSHV) gB dictates a low-pH endocytotic entry pathway as revealed by a dual-fluorescent virus system and a rhesus monkey rhadinovirus expres... by Shanchuan Liu, Sarah Schlagowski, Anna K Großkopf, Natalia Khizanishvili, Xiaoliang Yang, Scott W Wong, Elina M Guzmán, Marija Backovic, Stefano Scribano, Arne Cordsmeier, Armin Ensser, Alexander S Hahn

“…Single- and dual-fluorescent KSHV strains eliminate the need for virus-specific antibodies and enable the tracking of single viral particles during entry and fusion. …”
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Human Neutrophil Antigen Genotype and Allele Frequencies in Iranian Blood Donors by Behnaz Esmaeili, Behnaz Bayat, Atefe Alirezaee, Mona Delkhah, Mohammad Reza Mehdizadeh, Zahra Pourpak

“…Human neutrophil antigens (HNAs) can be targeted by HNA-allo antibodies and cause a variety of clinical conditions such as transfusion-related acute lung injury (TRALI) and neonatal alloimmune neutropenia (NAIN). …”
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Predictors of radiological progression of rheumatoid arthritis while receiving conventional synthetic disease-modifying antirheumatic drugs by O. B. Iaremenko, G. M. Mykytenko

“…Rheumatoid factor (RF) was detected in 53.3 % of patients, and antibodies to cyclic citrullinated peptide (ACCP) – in 54.0 %. …”
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A novel tissue culture method to produce professional antigen-presenting cells from rainbow trout in vitro by Nguyen T. K. Vo, PhD, Lital Sever, PhD, Brian Dixon, PhD, Niels C. Bols, PhD

“…Using affinity-purified anti-trout MH IIα and MH IIβ primary antibodies that were generated in house, abundant levels of MH IIα and MH IIβ polypeptides were detected in fin-LLCs by Western blotting. …”
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A Pregnant Woman with Excess Vasopressinase-Induced Diabetes Insipidus Complicated by Central Diabetes Insipidus like Lymphocytic Infundibulo-Neurohypophysitis by Shinnosuke Yanagisawa, Yoichi Oikawa, Mai Endo, Kazuyuki Inoue, Ritsuko Nakajima, Shigemitsu Yasuda, Masayasu Sato, Naoko Iwata, Haruki Fujisawa, Atsushi Suzuki, Yoshihisa Sugimura, Masashi Isshiki, Akira Shimada

“…The presence of anti-rabphilin-3A antibodies in the patient’s blood, a hypertonic saline infusion test result, and loss of the high-intensity signal of the posterior pituitary on fat-suppressed T1-weighted magnetic resonance images without thickening of the stalk and enlargement of the neurohypophysis suggested concurrent central DI-like lymphocytic infundibulo-neurohypophysitis (LINH). …”
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Implication of IL-17 in Bone Loss and Structural Damage in Inflammatory Rheumatic Diseases by Benoit Le Goff, Béatrice Bouvard, Thierry Lequerre, Eric Lespessailles, Hubert Marotte, Yves-Marie Pers, Bernard Cortet

“…Preliminary data from clinical trials showed a stabilisation of bone density in patients treated with anti-IL-17A antibodies. IL-17A plays a central role in the cartilage damage through the induction of collagenases and by decreasing the expression of their inhibitors in synergy with the other proinflammatory cytokines. …”
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Different MHC class I cell surface expression levels in diverse chicken lines, associations with B blood group, and proposed relationship to antigen-binding repertoire by Michael Kaiser, Jim Kaufman, Susan J. Lamont

“…In our study, erythrocytes from 6 diverse and highly inbred lines of chickens, a closed broiler line, and a highly advanced intercross line were evaluated for MHC class I antigen expression level by flow cytometry using monoclonal antibodies to chicken MHC class I molecules. In chickens, the B blood group antigens include the MHC class I antigen expressed from the MHC. …”
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Persistence with Early-Line Abatacept versus Tumor Necrosis Factor-Inhibitors for Rheumatoid Arthritis Complicated by Poor Prognostic Factors by Xue Han, Francis Lobo, Michael S. Broder, Eunice Chang, Sarah N. Gibbs, David J. Ridley, Irina Yermilov

“…**Objectives:** To assess real-world treatment persistence in early-line abatacept versus tumor necrosis factor-inhibitors (TNFi) treated patients with RA complicated by poor prognostic factors (including anti-cyclic citrullinated peptide antibodies \[ACPA\] and rheumatoid factor \[RF\] seropositivity). …”
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Immune status improvement in piglets through the use of interferon-containing products during specific prevention of porcine pleuropneumonia by A. G. Shakhov, L. Yu. Sashnina, V. A. Prokulevich, Yu. Yu. Vladimirova, M. I. Adodina

“…Tests for post-vaccination immunity levels in piglets showed a 3.8-fold increase in the levels of specific antibodies against the antigen of porcine pleuropneumonia agent, and in case of the vaccine administration in combination with biferon-S and prostimul – a 4.0-fold and 4.9-fold increase, respectively. …”
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Immune checkpoint inhibitors-related thyroid dysfunction: influencing factor analysis, prediction model development, and management strategy proposal by Xinya Li, Zaiwei Song, Yixuan Chen, Jingjing Wu, Dan Jiang, Zhen Zhang, Zeyuan Wang, Rongsheng Zhao

“…Results The systematic review encompassed 21 observational studies involving 4,145 patients, revealing associations between ICI-TD and factors such as female gender, age, receipt of Pembrolizumab (versus other ICIs), and baseline levels of thyroid-stimulating hormone, free thyroxine, and antithyroid antibodies. In the prediction model development phase, 621 participants were enrolled, with 36 patients developing ICI-TD. …”
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STAT3 Expression and Its Correlation with PD-L1 Expression in Non-Hodgkin’s Lymphoma and Hodgkin’s Lymphoma at Dr. Saiful Anwar Regional Public Hospital in Malang, Indonesian Popul... by Ailen Oktaviana Hambalie, Eviana Norahmawati, Agustina Tri Endharti, Diah Prabawati Retnani, Nayla Rahmadiani

“…Samples were obtained from paraffin blocks of patients with confirmed diagnoses of NHL and HL, and then immunohistochemical staining was carried out with PD-L1 and STAT3 antibodies. The collected data were then analyzed using SPSS. …”
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Subtype-specific human endogenous retrovirus K102 envelope protein is a novel serum immunosuppressive biomarker of cancer by Qinyuan Gong, Qinyuan Gong, Rongzhen Xu, Rongzhen Xu

“…We first generated monoclonal antibodies against K102-Env with high sensitivity and specificity, and we developed an ELISA assay to detect serum K102-Env. …”
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Persistently different patterns of patient’s global assessment of health in rheumatoid arthritis are associated with pain and impaired function more than with inflammation: an ince... by Sofia Ajeganova, Ingiäld Hafström, Valentina Bala, Björn Svensson

“…After adjustments, smoking and antibodies to cyclic citrullinated peptide status differentiated between being in low and high PGA groups. …”
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