Non-parathyroid hypercalcemia in a patient with new-onset hyperthyroidism and silicone-induced granulomas: case report by Laura Montefusco, Giada Rossi, Iulia Petria, Ida Pastore, Ida Pastore, Paolo Fiorina, Paolo Fiorina, Paolo Fiorina, Paolo Fiorina

“…Biochemical work-up revealed moderate hypercalcemia and overt hyperthyroidism with positive anti-TSH-receptor antibodies. Hydration, loop diuretics and methimazole were initiated immediately. …”
Get full text

Flow Cytometric Analysis of T, B, and NK Cells Antigens in Patients with Mycosis Fungoides by Serkan Yazıcı, Emel Bülbül Başkan, Ferah Budak, Barbaros Oral, Şaduman Balaban Adim, Zübeyde Ceylan Kalin, Güven Özkaya, Kenan Aydoğan, Hayriye Saricaoğlu, Şükran Tunali

“…Fluorescent labeled monoclonal antibodies were used to detect cell surface antigens: T cells (CD3+, CD4+, CD8+, TCRαβ+, TCRγδ+, CD7+, CD4+CD7+, CD4+CD7−, and CD71+), B cells (HLA-DR+, CD19+, and HLA-DR+CD19+), NKT cells (CD3+CD16+CD56+), and NK cells (CD3−CD16+CD56+). …”
Get full text

Inferior Outcomes of Fludarabine–Cyclophosphamide–Rituximab Chemotherapy in Korean Chronic Lymphocytic Leukemia Patients with Concurrent Thrombocytopenia and Anemia by Tong-Yoon Kim, Gi-June Min, Young-Woo Jeon, Seung-Ah Yahng, Seok-Goo Cho, Jong-Mi Lee, Myungshin Kim, Ki-Seong Eom

“…<b>Background/Objectives</b>: Anti-CD20 monoclonal antibodies combined with alkylator-based chemotherapy enhance survival in chronic lymphocytic leukemia (CLL). …”
Get full text

Akt/p27kip1 Pathway Is Not Involved in Human Insulinoma Tumorigenesis by Adriana Graciela Díaz, Andrea Paes de Lima, Paula Garibaldi, Maria de los Milagros Rubio, Florencia García, Marta Kral, Oscar D. Bruno

“…Staining was performed on embedded paraffin tissue using polyclonal antibodies against total Akt, p-Akt, p27kip1, and pp27kip1. p-Akt was the predominant form in insulinomas; they presented lower Akt and p-Akt expression than normal islets in 83.3% and 87.5% of tumors, respectively. p27kip1 and pp27kip1 were mainly cytoplasmic in both insulinomas and normal tissue. …”
Get full text

Immunophenotypic Characterization of Human Bone Marrow Mast Cells. A Flow Cytometric Study of Normal and Pathological Bone Marrow Samples by Luis Escribano, Alberto Orfao, Jesús Villarrubia, Beatriz Díaz-Agustín, Carlos Cerveró, Agustín Rios, José L. Velasco, Juana Ciudad, José L. Navarro, Jesús F. San Miguel

“…The goal of the present paper was to define the immunophenotype of bone marrow mast cells (BMMC) from healthy controls and patients with hematologic malignancies (HM) based on the use of multiple stainings with monoclonal antibodies analyzed by flow cytometry. Our results show that BMMC from both groups of individuals display a similar but heterogenous immunophenotype. …”
Get full text

Iodine-131 Combined With Plasma Exchange Treatment in Graves’ Hyperthyroidism Patients With Severe Liver Injury Whose Average Model for End-Stage Liver Disease Scores >20 by Xin-Fang Zhu, Rong-Rong Ding, Bing-Yao Wang, Yun-hui Yang, Fu-Xia Ta, Yuan Wang, Qing-Mei Gao, Qi Zhang, Rong Xia, Xing-Guang Luo, Xuan Wang, Jian-Ming Zheng, Hui-Qing Zhu

“…The levels of hemoglobin, platelet count, alanine aminotransferase, aspartate aminotransferase, total bilirubin, direct bilirubin, alkaline phosphatase, glutamyl transpeptidase, MELD scores, free triiodothyronine, free thyroxine, antithyroid peroxidase autoantibody and serum thyrotropin receptor antibodies after PE treatment were significantly lower than before PE treatment (P < .05). …”
Get full text

Caerin 1.1/1.9-mediated antitumor immunity depends on IFNAR-Stat1 signalling of tumour infiltrating macrophage by autocrine IFNα and is enhanced by CD47 blockade by Junjie Li, Yuandong Luo, Quanlan Fu, Shuxian Tang, Pingping Zhang, Ian H. Frazer, Xiaosong Liu, Tianfang Wang, Guoying Ni

“…In this study, we explored the impact of F1/F3 on the tumor microenvironment in a transplanted B16 melanoma model, and further investigated the mechanism of action of F1/F3 using monoclonal antibodies to deplete relevant cells, gene knockout mice and flow cytometry. …”
Get full text

A new vaccination approach for Salmonellosis employing a multi-epitope vaccine based on live microbial cell factory from Lactococcus lactis by Reyhaneh Sadat Moosavi-Kohnehsari, Mahnaz Jafari-Sohi, Tohid Piri-Gharaghie, Shakiba Tolou-Shikhzadeh-Yazdi, Mona Aghassizadeh-Sherbaf, Romina Hosseinzadeh

“…Birds that received the L. lactis/pNZ8121-Multi epitope vaccination showed increased levels of interferon (IFN-γ) and NFkB1α, increased transcription rates of cytokines, and a significant presence of IgY antibodies specific to the multi epitope gene in their serum. …”
Get full text

Citrullinated Antigens with Multiple Citrulline Similar Motif in the Diagnosis of Rheumatoid Arthritis: A Preliminary Single-Center Study by Zhu Chen, Weiting Fang, Yi Qin, Yin-Zhao Jin, Xiang Yang, Jianrong Lou

“…The presence of anti-citrullinated protein antibodies (ACPAs) in the serum is one of the immunological features of rheumatoid arthritis (RA). …”
Get full text

African swine fever in the Primorsky Krai: disease situation and molecular and biological properties of the isolate recovered from a wild boar long bone by А. R. Shotin, A. S. Igolkin, Ali Mazloum, I. V. Shevchenko, N. S. Bardina, E. O. Morozova, А. А. Shevtsov

“…The ASFV genome was detected in blood samples collected from infected pigs on 5–8 days post infection by real-time polymerase chain reaction. Specific antibodies in blood samples were not detected. The need in further research of molecular and biological properties of current ASFV isolates was reaffirmed. …”
Get full text

Transcriptome signature in the blood of neuromyelitis optica spectrum disorder under steroid tapering by Ryohei Yamamura, Makoto Kinoshita, Yoshiaki Yasumizu, Yoshiaki Yasumizu, Yoshiaki Yasumizu, Tomohiro Yata, Keigo Kihara, Daisuke Motooka, Daisuke Motooka, Naoyuki Shiraishi, Yasuko Sugiyama, Shohei Beppu, Hisashi Murata, Naoshi Koizumi, Itsuki Sano, Toru Koda, Tatsusada Okuno, Hideki Mochizuki

“…All patients were positive for anti-AQP4 antibodies and had no history of biologic use.ResultsIn one relapsed patient, gene groups with decreased expression at relapse were observed predominantly in monocytes, with upregulation in anti-inflammatory pathways such as IL-10, while the upregulated genes were related to interferon signaling. …”
Get full text

Jet Injection of Naked mRNA Encoding the RBD of the SARS-CoV-2 Spike Protein Induces a High Level of a Specific Immune Response in Mice by Denis N. Kisakov, Larisa I. Karpenko, Lyubov A. Kisakova, Sergey V. Sharabrin, Mariya B. Borgoyakova, Ekaterina V. Starostina, Oleg S. Taranov, Elena K. Ivleva, Oleg V. Pyankov, Anna V. Zaykovskaya, Elena V. Dmitrienko, Vladimir A. Yakovlev, Elena V. Tigeeva, Irina Alekseevna Bauer, Svetlana I. Krasnikova, Nadezhda B. Rudometova, Andrey P. Rudometov, Artemiy A. Sergeev, Alexander A. Ilyichev

“…It was demonstrated that mice vaccinated with naked mRNA-RBD developed a high level of specific antibodies with virus-neutralizing activity. The vaccine also induced a strong RBD-specific T-cell response and reduced the viral load in the lungs of the animals after infection with the SARS-CoV-2 virus. …”
Get full text

Viral diversity in wild rodents in the regions of Canaã de Carajás and Curionopólis, State of Pará, Brazil by Adriana Freitas Moraes Monteiro, Adriana Freitas Moraes Monteiro, Fábio Silva da Silva, Ana Cecília Ribeiro Cruz, Ana Cecília Ribeiro Cruz, Sandro Patroca da Silva, Alice Louize Nunes Queiroz, Livia Medeiros Neves Casseb, Livia Medeiros Neves Casseb, Livia Carício Martins, Livia Carício Martins, Daniele Barbosa de Almeida Medeiros, Daniele Barbosa de Almeida Medeiros

“…A total of 102 rodent samples were taxonomically identified through karyotyping and screened for anti-Orthohantavirus antibodies using the ELISA method. Subsequently, nucleotide sequencing and bioinformatics analyses were conducted on 14 selected samples to characterize the virome. …”
Get full text

Toxoplasma gondii in dromedary camels (Camelus dromedarius) in Egypt: a comparative seroepidemiological study in Upper and Lower Egypt by Ehab Kotb Elmahallawy, Ehab Kotb Elmahallawy, Nady Khairy Elbarbary, David Cano-Terriza, David Cano-Terriza, Tomás Fajardo, Nada Oudah Albalawi, Débora Jiménez-Martín, Marwa M. I. Ghallab, Ahmed Gareh, Refaat Ras, Refaat Ras, Isabelle Villena, Sabry A. S. Sadek, Hajar AlQadeeb, Hind Alzaylaee, Sonia Almería, Ignacio García-Bocanegra, Ignacio García-Bocanegra

“…The Modified Agglutination Test (MAT) was performed to screen for anti-T. gondii antibodies. The overall seroprevalence was 13.84% (71/513; 95CI%:10.85–16.83). …”
Get full text

Troglitazone and Δ2Troglitazone Enhance Adiponectin Expression in Monocytes/Macrophages through the AMP-Activated Protein Kinase Pathway by Jaw-Shiun Tsai, Lee-Ming Chuang, Ching-Shih Chen, Chan-Jung Liang, Yuh-Lien Chen, Ching-Yu Chen

“…TG and Δ2TG decreased the adhesion of THP-1 cells to TNF-α-treated HUVECs and the inhibitory effect was abolished by specific antiadiponectin antibodies. TG- and Δ2TG-induced suppression on monocyte adhesion were inhibited by a selective AMPK inhibitor compound C. …”
Get full text

Preparation of recombinant myoglobin and investigation of the liquid antigen stability for quality control materials by Yu-Hui Wang, Xi-Feng Sun, Chun-Xin Xu, Feng-Qiang Sun, Rong-Rong Wang, Xiao-Kun Bian, Zhan-Zhao Wang, Qiang Wu

“…The concentrations per gradient measured using different kits had no significant difference (p-value>0.05), which indicated that the reactivity between the Mb antigen and Mb antibodies with different epitopes was good. The effects of different storage buffer, storage temperature and storage times on the stability of liquid Mb were investigated by detecting the concentration changes. …”
Get full text

A core set of risk factors in individuals at risk of rheumatoid arthritis: a systematic literature review informing the EULAR points to consider for conducting clinical trials and... by Daniel Aletaha, Kulveer Mankia, Paul Emery, Deshire Alpizar-Rodriguez, Andreas Kerschbaumer, Andrea Di Matteo, Heidi Siddle, Joel Kerry

“…There was good evidence for serum anticitrullinated protein antibodies (ACPA) levels, as risk factors for arthritis in all at-risk populations (n=13 articles). …”
Get full text

The 2023 dengue outbreak in Lombardy, Italy: A one-health perspective by Francesca Rovida, Marino Faccini, Carla Molina Granè, Irene Cassaniti, Sabrina Senatore, Eva Rossetti, Giuditta Scardina, Manuela Piazza, Giulia Campanini, Daniele Lilleri, Stefania Paolucci, Guglielmo Ferrari, Antonio Piralla, Francesco Defilippo, Davide Lelli, Ana Moreno, Luigi Vezzosi, Federica Attanasi, Marzia Soresini, Manuela Barozzi, Lorenzo Cerutti, Stefano Paglia, Angelo Regazzetti, Maurilia Marcacci, Guido Di Donato, Marco Farioli, Mattia Manica, Piero Poletti, Antonio Lavazza, Maira Bonini, Stefano Merler, Fausto Baldanti, Danilo Cereda, Antonella Sarasini, Milena Furione, Dalila Mele, Federica Bergami, Josè Camilla Sammartino, Alessandro Ferrari, Greta Romano, Antonino Maria Guglielmo Pitrolo, Maya Carrera, Rita Brugnoli, Nunzia Laini, Francesca Bonalda, Sara Arfani, Giuditta Zamboni, Fanny Delfanti, Piergiuseppe Ferrari, Anxhela Dafa, Antonella Negri, Filippa Parisi, Marcello Tirani, Michela Viscardi, Gabriele Del Castillo, Federica Morani, Francesco Scovenna, Sheila Sansebastian, Manuel Maffeo, Mario Chiari, Enrico Tallarita

“…A serological screening for DENV antibodies detection was offered to the population. …”
Get full text

Antitumor effects of natural killer cells derived from gene-engineered human-induced pluripotent stem cells on hepatocellular carcinoma by Mayuna Nakamura, Yuka Tanaka, Keishi Hakoda, Masahiro Ohira, Tsuyoshi Kobayashi, Kenji Kurachi, Kouichi Tamura, Hideki Ohdan

“…The TRAIL and perforin/granzyme B pathways are largely involved in this cytotoxic mechanism, as indicated by the reduction in cytotoxicity induced by TRAIL inhibitory antibodies and concanamycin A, which inhibits perforin/granzyme B-mediated cytotoxicity. …”
Get full text

Acute therapeutic effects and pathophysiology of eosinophilic granulomatosis with polyangiitis neuropathy by Fumitaka Shimizu, Mariko Oishi, Toshihiko Maeda, Yukio Takeshita, Hideaki Nishihara, Ryota Sato, Takashi Kanda, Masayuki Nakamori, Michiaki Koga, Miwako Fujisawa, Yuki Mizumoto, Nanami Yamanaka, Takashi Nawata, Tatsuya Okafuji, Susumu Fujikawa, Kinya Matsuo, Joe Nemoto, Masaya Honda

“…Antineutrophil cytoplasmic antibodies (ANCA)-negative cases showed significantly higher recovery rates than ANCA-positive cases. …”
Get full text