Protocol for high-yield bacterial expression and purification of the voltage-dependent anion channel 1 for high-throughput biophysical assays by Stefano Conti Nibali, Andrea Magrì, Angela Messina, Armin Wagner, Ramona Duman, Vito De Pinto, Cristian Turato, Cristina Arrigoni, Marco Lolicato

“…In this context, we demonstrate how 2-methyl-2,4-pentanediol (MPD), a crystallization reagent, interferes with VDAC1 small-molecule binding, hindering the detection of these ligands in the crystal.For complete details on the use and execution of this protocol, please refer to Conti Nibali et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol to detect neutral lipids with BODIPY staining in myeloid-derived suppressor cells in mouse mammary tumors by Ukjin Kim, Rumela Chakrabarti

“…Furthermore, we introduce alternative protocols with MDSC sorting to overcome risk of cell death by BODIPY.For complete details on the use and execution of this protocol, please refer to Kim et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol for transcriptomic and epigenomic analyses of tip-like endothelial cells using scRNA-seq and ChIP-seq by Shintaro Funasaki, Yuri Miyamura, Shunsuke Kamei, Akhinur Rahman, Masaya Yamazaki, Shingo Usuki, Keiichiro Yasunaga, Yorifumi Satou, Hiroto Ohguchi, Takashi Minami

“…This scalable protocol is also applicable to diverse omics studies, including proteomics and metabolomics.For complete details on the use and execution of this protocol, please refer to Miyamura et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells by Sherry Li Zheng, Jonas L. Fowler, Julie Y. Chen, Christopher Li, Elaine Lin, Alana T. Nguyen, Angela Chen, George Q. Daley, Lay Teng Ang, Kyle M. Loh

“…This 10-day protocol enables the manufacturing of blood and immune cells in monolayer cultures.For complete details on the use and execution of this protocol, please refer to Fowler and Zheng et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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From union clout to corporate couture: Unveiling the impact of trade unions on corporate social responsibility. by Anshul Mandliya, Jatin Pandey, Shrihari Suresh Sohani, Rayees Ahmad Sheikh

“…Trade unions and corporate social responsibility (CSR) are important institutional mechanisms through which corporations undertake responsibility for their for-profit as well as not-for-profit actions. …”
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European Integration Influence on the Development of Human Capital of Small and Medium Enterprises in Poland by Marzena Kacprzak, Agnieszka Król, Marta Radziszewska

“…Entrepreneurs willingly undertake to raise funds from EU funds. Re-spondents were convinced that EU funds help in the development of their businesses, although ob-taining funding alone is not easy. …”
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Protocol for detecting eDNA in ecological rare fish using RPA-CRISPR-Cas12a technology by Xing-Yi Wei, Yang Pei, Li Liu, Péter Hamar, De-Sheng Pei

“…This protocol offers a high-sensitivity approach for monitoring aquatic biodiversity and conservation efforts, even in low eDNA concentrations.For complete details on the use and execution of this protocol, please refer to Wei et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol for predicting the single-cell network-based gene activity landscape during human B cell development by Xin Huang, Xuetong Hou, Yizhen Li, Jun J. Yang, Jiyang Yu

“…Our protocol elucidates the biological characteristics of developmental stages in human B cells.For complete details on the use and execution of this protocol, please refer to Huang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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FinTech: a literature review of emerging financial technologies and applications by Gang Kou, Yang Lu

“…Within the financial milieu, there is an ongoing pursuit for more precise, optimized, secure, and agile solutions capable of managing multifarious financial undertakings. Emerging technologies present remarkable modalities for achieving these objectives. …”
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Transformation of school assessment. Approaches within an educational innovation process: A cross-case analysis of schools in Catalonia (Spain) by David Martinez Maireles

“…A multiple case study design was employed to examine assessment framework modifications in seven Catalan schools undertaking significant pedagogical reform initiatives. …”
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Protocol for odorant-binding protein 2A gene knockdown by dual siRNA transfection in a three-dimensional human epidermal equivalent model by Shinobu Nakanishi

“…The efficiency of gene knockdown is verified by qPCR and ELISA.For complete details on the use and execution of this protocol, please refer to Nakanishi et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Regional Dimension of history of Organs of GPU-OGPU: to the analysis of monograph by A. B. Gularyan and A. Yu. Sarana by A. V. Sushko

“…It is concluded that Russian historians should support and continue such an undertaking, expanding its scope geographically and chronologically. …”
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Planning and Design of Equitable Free-Floating Bike-Sharing Systems Implementing a Road Pricing Strategy by Leonardo Caggiani, Rosalia Camporeale, Michele Ottomanelli

“…One possible strategy to undertake in order to achieve this objective is the implementation of a road pricing: tolling private cars when passing by certain roads of the network could be a way to tone down the traffic congestion and, at the same time, encourage the shifting towards more sustainable means of transport. …”
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Protocol for predicting host-microbe interactions and their downstream effect on host cells using MicrobioLink by Lejla Gul, Anna Julia Elias, Tanvi Tambaku, Marton Olbei, Emily Watters, Balazs Bohar, Dezso Modos, Matthew Madgwick, Tamas Korcsmaros

“…The protocol outlines procedures for predicting protein-protein interactions through domain-motif interactions, integrating multi-omic datasets to map downstream effects, performing network analyses to identify key regulatory pathways, and visualizing multi-layered networks for systems-level data interpretation.For complete details on the use and execution of this protocol, please refer to Gul et al.1 and Poletti et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Methods Dealing with Complexity in Selecting Joint Venture Contractors for Large-Scale Infrastructure Projects by Ru Liang, Zhaohan Sheng, Xiangyu Wang

“…This paper is to study how to select joint venture contractors for undertaking large-scale infrastructure projects based on a multiattribute mathematical model. …”
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Protocol for profiling RNA m5C methylation at base resolution using m5C-TAC-seq by Xiaoting Zhang, Liang Lu, Chengqi Yi, Xiaoyu Li

“…We detail steps for TET-assisted chemical labeling, library construction, and data analysis. m5C-TAC-seq enables accurate and robust m5C detection in various RNA species.For complete details on the use and execution of this protocol, please refer to Lu et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Protocol for live imaging of axonal transport in iPSC-derived iNeurons by Dan Dou, Erika L.F. Holzbaur, C. Alexander Boecker

“…Our protocol is optimized for the widely available catalog of KOLF2.1J iPSCs with mutations relevant to neurodegenerative diseases but is also applicable to other iPSC lines.For complete details on the use and execution of this protocol, please refer to Dou et al.1,2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Stress Monitoring in Pandemic Screening: Insights from GSR Sensor and Machine Learning Analysis by Antonios Georgas, Anna Panagiotakopoulou, Grigorios Bitsikas, Katerina Vlantoni, Angelo Ferraro, Evangelos Hristoforou

“…An attempt was made to measure the level of anxiety of individuals undertaking rapid tests for SARS-CoV-2. To this end, a galvanic skin response (GSR) sensor that was connected to a microcontroller was used to record the individual stress levels. …”
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Positive experiences of distance education from the perspective of primary school pupils, grades I–III by Agnieszka Koterwas, Edyta Nowosielska

“…In both spaces, compared to education within the walls of a school, firstly, students had a wider range of different activities possible to undertake, and secondly, the teacher’s directive nature in the learning process was limited. …”
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Friedrich Nietzsche, der "Meister des Verdachts". Über die Nietzsche-Lektüren von Michel Foucault und Paul Ricoeur by Yvanka Raynova

“…The author therefore asks herself where this thematic agreement comes from and undertakes a comparison of Foucault's and Ricoeur's readings of Nietzsche. …”
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