Mapping dynamic regulation of gene expression using single-cell transcriptomics and application to complex disease genetics by Hanna Abe, Phillip Lin, Dan Zhou, Douglas M. Ruderfer, Eric R. Gamazon

“…However, the identification of gene-level associations in distinct cell types faces several challenges, including the limited reference resources from population-scale studies, data sparsity in single-cell RNA sequencing, and the complex cell state pattern of expression within individual cell types. …”
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Biphasic biomimetic scaffolds based on a regionally decalcified bone framework and pre-chondrogenic microspheres for osteochondral defect repair by Zhuo Liang, Qingqing Pan, Fei Xue, Jingdi Zhang, Zhenlin Fan, Weiyun Wang, Xueqiang Guo, Zhuang Qian, Yaping Shen, Wenjuan Song, Lei Wang, Guangdong Zhou, Yong He, Wenjie Ren

“…Osteochondral defects are still facing a significant challenge in clinical surgery, making post-trauma repair difficult. …”
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Processing Window and Microstructure of NiCoCrAlY Coating Deposited on Cast Iron Using Multilayer Laser Cladding by Hao Liu, Peijian Chen, Haifeng Yang, Jingbin Hao, Xianhua Tian, Xiuli He, Gang Yu

“…Nickel-base or cobalt-base alloy coatings prepared by laser cladding can improve the surface properties of cast iron, thereby increasing the service life of the components; however, due to the poor weldability and high carbon content of cast iron, the use of laser cladding to prepare alloy coatings faces many difficulties. To reduce the brittleness of the bonded interface, laser cladding was applied to the surface of cast iron using a multilayer deposition strategy. …”
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Integrated Assessment of Productive, Environmental, and Social Performances of Adopting Low-Protein Diets Technology for Laying Hens by Dongsheng Li, Xiaoying Zhang, Zhiyang Zhao, Siqi Wang, Jing Wang, Hongliang Wang

“…China, the world’s largest egg producer, faces challenges of protein feed shortages and nitrogen pollution from manure. …”
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The trend of suicide and self-harm in the Chinese population from 2018 to 2022 based on ambulance medical emergency cases: a retrospective study by Ruizhe Yang, Jinsu Zhou, Francis Manyori Bigambo, Wu Yan, Xu Wang, Haibo Yang

“…Additionally, training healthcare providers and promoting family education can enhance support for women facing mental health challenges.…”
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Accuracy and Usability of Smartphone-Based Distance Estimation Approaches for Visual Assistive Technology Development by Giles Hamilton-Fletcher, Mingxin Liu, Diwei Sheng, Chen Feng, Todd E. Hudson, John-Ross Rizzo, Kevin C. Chan

“…<italic>Methods:</italic> We tested five smartphone-based distance-estimation approaches in the image center and periphery at 1&#x2013;3 meters, including machine learning (CoreML), infrared grid distortion (IR_self), light detection and ranging (LiDAR_back), and augmented reality room-tracking on the front (ARKit_self) and back-facing cameras (ARKit_back). <italic>Results:</italic> For accuracy in the image center, all approaches had &lt;&#x00B1;2.5 cm average error, except CoreML which had &#x00B1;5.2&#x2013;6.2 cm average error at 2&#x2013;3 meters. …”
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The assessment of dairy goats’ welfare in alpine summer ranges by Manuela Renna, Benedetta Torsiello, Silvana Mattiello, Luca Battaglini, Monica Battini

“…IOR of individual-level indicators was excellent or good, but unacceptable for hair coat condition, probably due to insufficient light conditions that sometimes the assessors faced inside the milking pens. IOR of group-level indicators was unacceptable in most cases. …”
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Harnessing AI for sustainable higher education: ethical considerations, operational efficiency, and future directions by Sunawar Khan, Tehseen Mazhar, Tariq Shahzad, Muhammad Amir Khan, Ateeq Ur Rehman, Mamoon M. Saeed, Habib Hamam

“…Abstract As higher education faces technological advancement and environmental imperatives, AI becomes a key instrument for revolutionizing instructional methods and institutional operations. …”
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Cyanobacteria cultivation using olive milling wastewater for bio-fertilization of celery plant by S. Rashad, A.S. El-Hassanin, S.S.M. Mostafa, G.A. El-Chaghaby

“…Olive milling wastewater is a major problem facing the Mediterranean countries producing olive oil like Egypt. …”
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Do indigenous people get left behind? An innovative methodology for measuring the unmeasurable economic conditions and poverty from the poorest region of Luzon, Philippines by Emmanuel A. Onsay, Jomar F. Rabajante

“…The study underscores the urgency of targeted policies and programs to address multidimensional deprivations faced by Indigenous communities, emphasizing the importance of data-driven approaches in improving welfare and promoting equitable socio-economic progress. …”
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France and The European Charter for Regional or Minority Languages: Dilemma without Solution by E. V. Ermakova

“…The question of amending the Constitution of France is facing fierce debate and remains unresolved for nearly a decade. …”
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Care-seeking patterns and timely access to care among survivors of sexual violence in North Kivu, the Democratic Republic of the Congo: a retrospective file-based study by Hanna Reinholdz, Anette Agardh, Meggy Verputten, Joseph Byenda, Helena Frielingsdorf

“…Through a better understanding of care-seeking behaviours and factors influencing timely access to care, programmes can be adapted to overcome some of the barriers faced by survivors of sexual violence. Objective The aim of this study was to describe demographics, care-seeking patterns and factors influencing timely care-seeking by survivors of sexual violence. …”
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Emergency medical service interventions and experiences during pandemics: A scoping review. by Despina Laparidou, Ffion Curtis, Nimali Wijegoonewardene, Joseph Akanuwe, Dedunu Dias Weligamage, Prasanna Dinesh Koggalage, Aloysius Niroshan Siriwardena

“…<h4>Conclusions</h4>Despite concerns for their own and their families' safety and the many challenges they are faced with, especially their knowledge, training, lack of appropriate Personal Protective Equipment (PPE) and constant protocol changes, EMS personnel were willing and prepared to report for duty during pandemics. …”
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Expanding the role of non-physician medical staff in primary care in Germany: protocol for a mixed-methods study exploring the perspectives of physicians in rural practices by Joachim E Fischer, Heiner Averbeck, David Litaker

“…Introduction Primary care faces substantial challenges worldwide through an increasing mismatch in supply and demand, particularly in rural areas. …”
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EMERGENCY DISTANCE EDUCATION AS A SOCIAL COMMUNICATION STRATEGY IN UKRAINE AND THE WORLD by Nadiya Figol, Yuriy Kolisnyk, Tetiana Faichuk, Bogdan Figol

“…The article also discusses key challenges faced by educational institutions during the transition to EDL, including the technical and methodological unpreparedness of educators, the lack of necessary infrastructure, and limited access to the internet and digital resources in some regions. …”
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Impact of generalized joint hypermobility on quality of life and physical activity in school-aged children: a longitudinal study by Oluwakemi A. Ituen, Christie D. Akwaowo, Gillian Ferguson, Jacques Duysens, Bouwien Smits-Engelsman

“…The children were administered the FACES pain scale, the child activity limitation interview, a physical activity questionnaire, and the pediatric quality of life inventory questionnaire. …”
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Improving fluoroprobe sensor performance through machine learning by D. Lafer, A. Sukenik, T. Zohary, O. Tal

“…However, applying the FP has faced challenges, leading to inaccuracies in phytoplankton assessment. …”
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Energy Saving Technologies and Practices in Facility Agriculture in Cold Regions by Ningning Li, Weiyao Xu, Yue Zhang, Wenchuan Ma, Yanjie Ren

“…However, cold region facility agriculture faces significant energy and environmental challenges, necessitating the advancement and application of energy saving technologies. …”
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Green Hydrogen for Energy Transition: A Critical Perspective by Ruggero Angelico, Ferruccio Giametta, Biagio Bianchi, Pasquale Catalano

“…However, widespread adoption faces challenges, including high production costs, infrastructure constraints, and the need for robust regulatory frameworks. …”
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Quality control and safety management of oil and gas pipelines during its whole life cycle by Changliang JIANG

“…Besides, analysis was conducted on the challenges faced by the construction quality and operation safety of oil and gas pipelines. …”
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