Molecular Detection and Multidrug Resistance of Shigella spp. Isolated from Wild Waterfowl and Migratory Birds in Bangladesh by Jarna Karmoker, Md. Saiful Islam, Md. Liton Rana, Md. Ashek Ullah, Fahim Haque Neloy, Nahian Muniath Oishy, Pritom Kumar Pramanik, Mahbubul Pratik Siddique, Sukumar Saha, Md. Tanvir Rahman

“…Antibiotic resistance genes tetA and SHV were found in 69.23% and 50% of relevant antibiotic-resistant Shigella spp. isolates, respectively. …”
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A vita minimuma by Kosztasz Rosta

“… Az európai filozófiatörténet vélhetőleg első „metavitájában” a beszélgetőpartnerek megszakítják eszmecseréjüket a tárgyról és a vita mikéntje kapcsán próbálnak egyezségre jutni: Szókratész a rövid, kérdés-felelet formájában lezajló beszélgetést preferálja, mely jól definiált propozíciókat, illetve ezek logikai térképét eredményezi, míg Prótagorasz szíve szerint inkább bemutató beszédek versenyében venne részt, melyben a nyelv nem reprezentacionalista, hanem tropikus/metaforikus, és nagyobb, kumulatív egységeket alkot, mint a propozíciók. A tét tehát nem pusztán annyi, hogy milyen hosszan fejthessék ki álláspontjukat a felek, hanem hogy a gondolkodás két, nehezen összeegyeztethető útja közül melyiket használják. …”
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Multidrug Resistant Enteric Bacteria from Cancer Patients Admitted in Douala Laquintinie Hospital, Littoral Region of Cameroon by Michael F. Kengne, Ornella D. Tsobeng, Ballue S. T. Dadjo, Victor Kuete, Armelle T. Mbaveng

“…Proteus vulgaris, the most prevalent isolate, showed significantly high resistance (with p<0.05) in cancer patients compared to noncancer patients at amoxicillin/clavuranic acid (AMC) (89.13% versus 41.30%), ceftriaxone (CTR) (63.04% versus 39.13%), ciprofloxacin (CIP) (65.22% versus 34.18%), and tetracycline (TET) (93.48% versus 63.04%). Multidrug resistance was observed in cancer patients compared to noncancer patients for Klebsiella pneumoniae (85.00% versus 60.00%), Salmonella typhi (84.62% versus 60.00%), and Klebsiella oxytoca (86.49% versus 43.48%). …”
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5‐Hydroxymethylcytosine Profiles of cfDNA in Urine as Diagnostic, Differential Diagnosis and Prognostic Markers for Multiple Myeloma by Weiwei Xie, Xuehui Li, Hangyu Chen, Jinlin Chu, Lei Zhang, Bo Tang, Wenrong Huang, Linlin Li, Jian Lin, Yujun Dong

“…In addition, the prognostic prediction model also effectively predicted the prognosis of patients with MM (p < 0.01), of which 4 differential markers (RAPGEF2, BRD1, TET2, TRAF3IP2) could independently predict the prognosis of MM. …”
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Evaluation of plasma cell sorting methods in multiple myeloma patients: flow cytometry versus magnetic beads by Yu Jeong Choi, Jaeguk Choi, Yehyun Kang, Saeam Shin, Seung-Tae Lee, Jong Rak Choi

“…Wilcoxon matched-pairs signed rank test analysis showed that the median of differences of variant allele frequency (VAF) of two methods (VAF of MACS minus VAF of FACS) in the DNMT3A, TET2, and ASXL1 (DTA) group was − 0.006555 (p = 0.0020), while that in the non-DTA group was 0.002805 (p = 0.0019). …”
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Abnormal methylation of Mill1 gene regulates osteogenic differentiation involved in various phenotypes of skeletal fluorosis in rats and methionine intervention by Niannian Chen, Jing Zhang, Congyu Yin, Yudan Liao, Lei Song, Ting Hu, Xueli Pan

“…Subsequently, we validated the regulation of Mill1 gene methylation using DNMT1 and TET2 enzyme inhibitors. Furthermore, we knockdown and overexpression experiments confirmed its downregulation inhibited osteogenic differentiation, whereas osteogenic differentiation was promoted by its overexpression. …”
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Phenotypic and WGS-derived antibiotic resistance patterns of Salmonella Enteritidis isolates from retail meat and environment during 2014 to 2019 in China by Liya Zheng, Qiannan Di, Xuebin Xu, Liyuan Liu, Chunbo Qu, Phil Bremer, Phil Bremer, Xiujuan Zhou

“…Most MDR strains (81.43%) had NAL-AMP-FIS-STR ± TET profiles, showing a fluctuating trend from 2015 to 2019, likely influenced by tetracycline withdrawal management. …”
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Novel target and cofactor repertoire for the transcriptional regulator JTY_0672 from Mycobacterium bovis BCG by Hui Wang, Xiaotian Li, Shuxian Wang, Ren Fang, Jiayin Xing, Ruiying Wu, Chunhui Zhang, Zhaoli Li, Ningning Song

“…However, the functionality of JTY_0672, a member of the TetR family of transcriptional regulators, remains unknown. …”
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Streptococcus suis-associated neonatal meningitis and sepsis: characterization, antimicrobial resistance, and public health implications by Giovanna Fusco, Rubina Paradiso, Lorena Cardillo, Maria Antonia Salvia, Saveria Dodaro, Veronica Del Monaco, Gianfranco Scarpelli, Francesca Greco, Antonio Rinaldi, Lorella Barca, Stefania Ambrogio, Antonio Limone, Esterina De Carlo, Giorgia Borriello

“…Next-generation sequencing revealed that the strain was serotype 2 sequence type 1, and contained the ermB and tet(W) genes, which are responsible for resistance to macrolides and tetracycline, along with several pilus-associated genes and 20 virulence factors. …”
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STAT3-Inducible Mouse ESCs: A Model to Study the Role of STAT3 in ESC Maintenance and Lineage Differentiation by Yu Qian Wong, Hongyan Xu, Qiang Wu, Xinyu Liu, Chengchen Lufei, Xiu Qin Xu, Xin-Yuan Fu

“…Therefore, in this study, two inducible STAT3 ESC lines were established, including the STAT3 knockout (InSTAT3 KO) and pSTAT3 overexpressed (InSTAT3 CA) using Tet-on inducible system in which STAT3 expression can be strictly controlled by doxycycline (Dox) stimulation. …”
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昆明滇池红嘴鸥源沙门氏菌的分离鉴定、耐药性及致病性分析 by 邬佳莉, 杨 勇, 相德才, 张文梅, 代飞燕, 段博芳, 段 纲

“…结果显示:确定其为ST19型鼠伤寒沙门氏菌(<i>Salmonella</i> Typhimurium),抗原结构式为O抗原1,4,[5],12; H抗原i,1,2,主要对阿莫西林、环丙沙星、四环素和多西环素耐药,且检出四环素类耐药基因<i>tetB,</i>喹诺酮类耐药基因<i>gyrA、gyrB、parC</i>和<i>psrE。</i>该分离菌检测出<i>mogA、sseL、mgtC、bcfA、araB、spvC、spvB、stn、fimA、orgA、sopB、sipB</i>和<i>spaN</i> 13种毒力基因。…”
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中华竹鼠源致病性大肠杆菌分离鉴定、耐药性及毒力基因检测 by 阎朝华 1, 2 张 旭 1, 2 陈国权 1, 2 周碧君 1, 2, 3 王开功 1, 2, 3 赵大杰 1, 2 文 明 1, 2, 3 张件军 1, 2

“…药敏试验结果显示,分离菌对氟苯尼考、头孢曲松、头孢氨苄等药物敏感、对四环素、多西环素、青霉素等药物表现耐药,4种耐药基因检测结果显示<i>Intl</i> 1(146 bp)、<i>tetA</i>(831 bp)2种耐药基因,与药敏表型相符。人工感染试验结果显示,该菌对小鼠有致病性。…”
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Whole-genome sequencing-based characterization of Salmonella enterica Serovar Enteritidis and Kentucky isolated from laying hens in northwest of Iran, 2022–2023 by Shirin Vakili, Mehri Haeili, Adel Feizi, Kiarash Moghaddasi, Maryam Omrani, Arash Ghodousi, Daniela Maria Cirillo

“…Other identified antimicrobial resistance genes (ARGs) including tetA, floR, sul1, dfrA1, aph(3′)-Ia and double gyrA and parC mutations conferring high-level ciprofloxacin resistance (CIPR) (MIC ≥ 16mg/L) were only found in S. …”
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Multimodal cell-free DNA whole-genome TAPS is sensitive and reveals specific cancer signals by Dimitrios V. Vavoulis, Anthony Cutts, Nishita Thota, Jordan Brown, Robert Sugar, Antonio Rueda, Arman Ardalan, Kieran Howard, Flavia Matos Santo, Thippesh Sannasiddappa, Bronwen Miller, Stephen Ash, Yibin Liu, Chun-Xiao Song, Brian D. Nicholson, Helene Dreau, Carolyn Tregidgo, Anna Schuh

“…Here, we develop a methodology for ctDNA detection using deep (80x) whole-genome TET-Assisted Pyridine Borane Sequencing (TAPS), a less destructive approach than bisulphite sequencing, which permits the simultaneous analysis of genomic and methylomic data. …”
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<i>Salmonella enterica</i> serovar Schwarzengrund: Distribution, Virulence, and Antimicrobial Resistance by Monique A. Felix, Jing Han, Bijay K. Khajanchi, Yasser M. Sanad, Shaohua Zhao, Steven L. Foley

“…AMR genes were found in 1269 (61.7%) of the isolates, with a total of 2478 AMR genes among the isolates, the most common being <i>aph(3″)-Ib</i> (N = 969, 47.1%), <i>tet(A</i>) (N = 190, 9.2%), and <i>sul2</i> (N = 150, 7.3%), which are responsible for resistance to aminoglycoside, tetracycline, and sulfonamide, respectively. …”
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Florfenicol Resistance in Enterobacteriaceae and Whole-Genome Sequence Analysis of Florfenicol-Resistant Leclercia adecarboxylata Strain R25 by Yuanyuan Ying, Fei Wu, Chongyang Wu, Yi Jiang, Min Yin, Wangxiao Zhou, Xinyi Zhu, Cong Cheng, Licheng Zhu, Kewei Li, Junwan Lu, Teng Xu, Qiyu Bao

“…The sequences sharing the greatest similarities to pLA-64 are the plasmids p02085-tetA of Citrobacter freundii and p234 and p388, both from Enterobacter cloacae. …”
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Mesenchymal Stem Cells Carrying Viral Fusogenic Protein p14 to Treat Solid Tumors by Inducing Cell–Cell Fusion and Immune Activation by Yao Wang, Xunlei Pang, Ruirui Li, Jiuzhou Chen, Chen Wen, Huihuang Zhu, Tingyu Long, Jianjie Li, Lijun Zheng, Youcai Deng, Junnian Zheng, Bo Xu

“…Cell viability assay was employed to assess the killing effects of MSCs carrying the p14 protein (MSCs-p14), which was also identified in the subcutaneous tumor models. Subsequently, the Tet-On system was introduced to enhance the controllability and safety of therapy. …”
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Development and validation of genome-informed and multigene-based qPCR and LAMP assays for accurate detection of Dickeya solani: a critical quarantine pathogen threatening the pota... by Shefali Dobhal, Gem Santillana, Michael J. Stulberg, Dario Arizala, Anne M. Alvarez, Mohammad Arif

“…Our study employed a whole-genome comparative approach, identifying unique target gene loci (LysR and TetR family of transcriptional regulators gene regions) and designing loop-mediated isothermal amplification (LAMP) and a multi-gene-based multiplex TaqMan qPCR assays for specific detection and differentiation of D. solani. …”
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Driving effect of P16 methylation on telomerase reverse transcriptase-mediated immortalization and transformation of normal human fibroblasts by Xuehong Zhang, Paiyun Li, Ying Gan, Shengyan Xiang, Liankun Gu, Jing Zhou, Xiaorui Zhou, Peihuang Wu, Baozhen Zhang, Dajun Deng, Jing Ni

“…A zinc finger protein-based P16-specific DNA methyltransferase (P16-Dnmt) vector containing a “Tet-On” switch was used to induce extensive methylation of P16 CpG islands in normal human fibroblast CCD-18Co cells. …”
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Detecting Genetic Associations between ATG5 and Lupus Nephritis by trans-eQTL by Yue-miao Zhang, Fa-juan Cheng, Xu-jie Zhou, Yuan-yuan Qi, Ping Hou, Ming-hui Zhao, Hong Zhang

“…Four trans-eSNPs were observed to be associated with susceptibility to LN (P < 0.05), including ANKRD50 rs17008504, AGA rs2271100, PAK7 rs6056923, and TET2 rs1391441, while seven other trans-eSNPs showed marginal significant associations (0.05 < P < 0.1). …”
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