Revisiting Fat Graft Harvesting and Processing Technique to Optimize Its Regenerative Potential by Karina Karina, MD, PhD, Johannes A. Biben, MD, Krista Ekaputri, MD, Grady Krisandi, BMed, Imam Rosadi, MSc, Siti Sobariah, BSc, Irsyah Afini, MBiomed, Tias Widyastuti, BSc, Alfida Zakiyah, BSc, Difky Ernanda, BSc

“…The use of fat grafting has expanded to include cell and tissue regeneration, necessitating investigations to ensure the viability of stromal and adipose-derived mesenchymal stem cells (ASCs) within the transferred fat parcels. …”
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Osteoblastic Differentiation and Mitigation of the Inflammatory Response in Titanium Alloys Decorated with Oligopeptides by Aroa Álvarez-López, Raquel Tabraue-Rubio, Rafael Daza, Luis Colchero, Gustavo V. Guinea, Martine Cohen-Solal, José Pérez-Rigueiro, Daniel González-Nieto

“…We also find that RGD is the best-performing peptide in in vitro cell cultures, enhancing adhesion, proliferation, and osteogenic differentiation of mesenchymal stem cells. To assess the in vivo effect of RGD-decorated Ti-6Al-4V implants, we develop a calvarial model in murine hosts. …”
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Gelatin/Poly (Lactic-Co-Glycolic Acid)/Attapulgite Composite Scaffold Equipped with Teriparatide Microspheres for Osteogenesis in vitro and in vivo by Zhao Z, Feng X, Zhao Y, Song Z, Zhang R, Zhang K, He Y, Chen G, Zhang J, Wang W

“…Mouse bone mesenchymal stem cells (BMSCs) were co-cultured with extracts from the microspheres and scaffolds to evaluate cell proliferation and osteogenesis. …”
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Long noncoding RNAs predict the survival of patients with colorectal cancer as revealed by constructing an endogenous RNA network using bioinformation analysis by Hui Zhang, Zhuo Wang, Jianzhong Wu, Rong Ma, Jifeng Feng

“…Functional enrichment analysis suggested that the target genes of the ceRNA network may be involved in the pathways related to cancer, including the signaling pathway that regulates the pluripotency of stem cells, wnt signaling pathway, hippo signaling pathway, basal cell carcinoma, and colorectal cancer. …”
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Exploring molecular disparities of H-type vasculature endothelial cells in osteonecrosis of the femoral head through single-cell analysis by Wei Shi, Dong Li, Qian Xu, Kai Zhang, Xinyu Liang, Hui Li, Zhijun Li, Huafeng Zhang

“…Results After integrating the 9 samples, 14 cell types were identified: B cells, Mesenchymal stem cells, Osteoblasts, Endothelial cells, Monocytes, T cells, NK cells, Fibroblasts, Macrophages, Common myeloid progenitors, Chondrocytes, Myelocytes, Osteoclasts, and Pericytes. …”
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Bioadhesive Chitosan Films Loading Curcumin for Safe and Effective Skin Cancer Topical Treatment by Seila Tolentino, Mylene M. Monteiro, Felipe Saldanha-Araújo, Marcilio Cunha-Filho, Tais Gratieri, Eliete N. Silva Guerra, Guilherme M. Gelfuso

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兔结膜基质诱导人骨髓间质干细胞分化的初步研究 by 黄丹平, 郑健梁, 高前应, 葛坚, 刘金陵

“…【目的】观察人骨髓间充质干细胞（mesenchymal stem cells，MSCs）在保存人羊膜及兔眼表结膜基质上分化的情况。【方法】24只新西兰兔随机分为2组。…”
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Review of Acupuncture and Moxibustion Treatment of Neurogenic Bladder after Spinal Cord Injury by Qianqian LIN, Chengmei LIU, Kewei CHEN, Huijie DUAN

“…Acupuncture can promote the proliferation of neural stem cells in patients with NB after SCI, so as to stimulate nerve regeneration and improve the function of the damaged nervous system; it can effectively induce the expression of neurotrophic factors and promote the repair of tissues after SCI; it can reduce the expression of pro-inflammatory and pro-apoptotic factors after SCI, so as to inhibit the apoptosis of nerve cells and protect nerve cells. …”
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CLINICAL SIGNS AND TREATMENT OF NEW-ONSET BONE MARROW FAILURE ASSOCIATED SARS-COV-2 INFECTION IN CHILDREN : A SINGLE INSTITUTION PROSPECTIVE COHORT STUD by Mervat A M youssef, Ebtisam Shawky hmed, Dalia Tarik Kamal, khalid Elsayh, Mai A Abdelfatta, Hyam Hassan Mahran Mahran, Mostafa M Embaby

“… Viral infections can cause direct and indirect damage to hematopoietic stem cells. The objectives of this study were to identify the frequency and severity of aplastic anemia in Severe acute respiratory syndrome coronavirus 2 ( SARS-CoV-2) infected children as well as recognition of the response to treatment Methodology: 13 children with newly-diagnosed severe aplastic anemia enrolled in this prospective clinical trial. …”
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Timosin alpha-1 immunomodulating properties by M. V. Mayevskaya

“…Immunomodulating effect of Timosin alpha-1 consists in increase of natural killer cells number, CD4 and CD8 by stimulation of stem cells differentiation and decrease of T-cells apoptosis, and also in driving of immune response to Th1 subtype by increase of Th1 cytokines (IL-2, IFN) amount and decreases of amount of Th2 cytokines (IL-4, IL-10). …”
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mTeSR1 is beneficial for the early development of bovine embryos and promotes the proliferation of the inner cell mass during the outgrowth of blastocysts by Dohyun Kim, HakKyo Lee, Dae-Jin Kwon

“…Background: mTeSR1 is a fully-defined, serum-free medium for the derivation and maintenance of Human embryonic stem cells (ESCs). This study investigates the impact of incorporating mTeSR1 supplement during in vitro culture (IVC) on blastocyst productivity, qualitative characteristics, and outgrowth potential of bovine blastocysts. …”
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The Endocannabinoid, Anandamide, Acts as a Novel Inhibitor of LPS-Induced Inflammasome Activation in Human Gastric Cancer AGS Cell Line: Involvement of CB1 and TRPV1 Receptors by Sahar Sadat Sedeighzadeh, Hamid Galehdari, Mohammad Reza Tabandeh, Mehdi Shamsara, Ali Roohbakhsh

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Review on the Injury and Repair Mechanism of Skeletal Muscle Contusions by LIN Jianping, WANG Hao, GUO Mingling, WANG Shizhong, CHEN Shaoqing

“…Notch signal pathway regulates the self-renewal and differentiation of myosatellite cells to maintain the homeostasis of muscle stem cells. And P13K/Akt/mTOR signal pathway has a positive regulation effect on myoblast differentiation of myosatellite cells and can promote skeletal muscle regeneration. …”
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Human Immune System Increases Breast Cancer-Induced Osteoblastic Bone Growth in a Humanized Mouse Model without Affecting Normal Bone by Tiina E. Kähkönen, Mari I. Suominen, Jenni H. E. Mäki-Jouppila, Jussi M. Halleen, Azusa Tanaka, Michael Seiler, Jenni Bernoulli

“…The study was performed using CIEA NOG (NOG) mice engrafted with human CD34+ hematopoietic stem cells (huNOG) and age-matched immunodeficient NOG mice. …”
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CalciumZero: a toolbox for fluorescence calcium imaging on iPSC derived brain organoids by Xiaofu He, Yian Wang, Yutong Gao, Xuchen Wang, Zhixiong Sun, Huixiang Zhu, Kam W. Leong, Bin Xu

“…However, a significant breakthrough in the field has emerged with the advent of utilizing patient-derived human induced pluripotent stem cells (iPSCs) to culture neurons and develop brain organoids. …”
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Novel insights into immunopathogenesis and crucial biomarkers between primary open‐angle glaucoma and systemic lupus erythematosus by Yixian Liu, Mengling You, Zhou Zeng, Jing Wang, Rong Rong, Xiaobo Xia

“…Finally, reverse transcription quantitative real‐time polymerase chain reaction (RT‐qPCR) was performed in human trabecular meshwork stem cells to validate the five identified biomarkers. …”
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Comprehensive analysis of inhibin-β A as a potential biomarker for gastrointestinal tract cancers through bioinformatics approaches by Rohit Kumar Verma, Prashant Kumar Srivastava, Ashutosh Singh

“…The KEGG pathway analysis revealed that INHBA and its interacting proteins are involved in several pathways, including TGF-beta signaling, Signalling pathways regulating pluripotency of stem cells, colorectal cancer, pancreatic cancer, AGE-RAGE signaling, and so on as major pathways. …”
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Fibroblasts in rheumatoid arthritis: novel roles in joint inflammation and beyond by Elpida Neofotistou-Themeli, Elpida Neofotistou-Themeli, Panagiota Goutakoli, Panagiota Goutakoli, Theodoros Chanis, Theodoros Chanis, Maria Semitekolou, Maria Semitekolou, Eirini Sevdali, Eirini Sevdali, Prodromos Sidiropoulos, Prodromos Sidiropoulos

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Polycaprolactone for Hard Tissue Regeneration: Scaffold Design and In Vivo Implications by Fernanda Ramírez-Ruiz, Israel Núñez-Tapia, María Cristina Piña-Barba, Marco Antonio Alvarez-Pérez, Vincenzo Guarino, Janeth Serrano-Bello

“…Additionally, the scaffolds synthesized can facilitate the inclusion of bone or mesenchymal stem cells with growth factors that improve bone osteogenesis, recruiting new cells for the neighborhood to generate an optimal environment for tissue regeneration. …”
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A miR-activated hydrogel for the delivery of a pro-chondrogenic microRNA-221 inhibitor as a minimally invasive therapeutic approach for articular cartilage repair by Shan An, Claudio Intini, Donagh O'Shea, James E. Dixon, Yiran Zheng, Fergal J. O'Brien

“…Our group has successfully developed an injectable hydrogel for cartilage repair, composed of collagen type I (Col I), collagen type II (Col II), and methacrylated-hyaluronic acid (MeHA), capable of supporting chondrogenic differentiation of mesenchymal stem cells (MSCs) towards articular cartilage-like phenotypes. …”
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