Scoulerine promotes cytotoxicity and attenuates stemness in ovarian cancer by targeting PI3K/AKT/mTOR axis by Wang Fang, Zhang Yang, Pang Rui, Shi Shaohong, Wang Ran

“…Scoulerine treatment also drastically reduced the spheroid-forming ability of OVCAR3 cells. The mesenchymal and stemness--related markers like N-cadherin, vimentin, CD-44, Oct-4, Sox-2 and Aldh1A1 were downregulated, whereas the epithelial markers like E-cadherin and CD-24 were upregulated in scoulerine-treated cells. …”
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Enhancing glioblastoma therapy: unveiling synergistic anticancer effects of Onalespib - radiotherapy combination therapy by Julia Uffenorde, Mehran Hariri, Eleftherios Papalanis, Annika Staffas, Josefine Berg, Bo Stenerlöw, Hanna Berglund, Christer Malmberg, Diana Spiegelberg, Diana Spiegelberg

“…In this study, we investigated the effect of HSP90 inhibition in combination with radiotherapy in established and patient-derived glioblastoma cell lines.MethodsPotential radiosensitizing effects of the HSP90 inhibitor Onalespib were studied in XTT and clonogenic survival assays as well as in tumor-mimicking multicellular spheroid models. Further, migration capacity and effects on protein expression were studied after exposure to Onalespib and radiation using Proximity Extension Assay analysis.ResultsHSP90 inhibition with Onalespib synergistically enhanced the radiosensitivity of glioblastoma cells grown in 2D and 3D models, resulting in increased cell death, reduced migration capacity and activation of the apoptotic signaling pathway. …”
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Developing novel Lin28 inhibitors by computer aided drug design by Victor M. Matias-Barrios, Mariia Radaeva, Graciella Rosellinny, Qiongqiong Jia, Ning Xie, Monica Villanueva, Hanadi Ibrahim, Jason Smith, Martin Gleave, Nada Lallous, Suzana K. Straus, Artem Cherkasov, Xuesen Dong

“…Ln268 suppressed Lin28-mediated cancer cell proliferation and spheroid growth. Results from nuclear magnetic resonance spectroscopy confirmed that Ln268 perturbs the conformation of the zinc knuckle domain of Lin28, validating the rational drug design by CADD. …”
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N6-methyladenosine-modification of USP15 regulates chemotherapy resistance by inhibiting LGALS3 ubiquitin-mediated degradation via AKT/mTOR signaling activation pathway in hepatoce... by Ronghuan Fang, Zhigang Jia, Yuhang Xin, Kai Zhao, Wei Qin, Haoran Lu, Yi Zhou, Yongsheng Yang, He Fang

“…Functional analyses of immortalized human liver and HCC cell lines were conducted, including quantitative real-time PCR; western blot; plasmid, lentivirus, and siRNA transfection; co-immunoprecipitation; mass spectrometry; MeRIP-qPCR; and ubiquitination, cell growth, colony formation, and spheroid formation assays. HCC tumor growth was also assessed using cell transplantation in nude mice. …”
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Treatment of a mutant KRAS lung cancer cell line with polyisoprenylated cysteinyl amide inhibitors activates the MAPK pathway, inhibits cell migration and induces apoptosis. by Matthew D Gregory, Kweku Ofosu-Asante, Jassy Mary S Lazarte, Pablo E Puente, Nada Tawfeeq, Nadine Belony, Yong Huang, Ite A Offringa, Nazarius S Lamango

“…The depletion of these key cytoskeletal proteins may account for the observed inhibition of cell migration and invasion, and spheroid invasion observed on exposure to NSL-YHJ-2-27 and NSL-YHJ-2-46. …”
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Magnolol induces cell death through PI3K/Akt‐mediated epigenetic modifications boosting treatment of BRAF‐ and NRAS‐mutant melanoma by Abdullah Al Emran, Brinda Reddy Chinna Chowdary, Farzana Ahmed, Heinz Hammerlindl, Antje Huefner, Nikolas K. Haass, Wolfgang Schuehly, Helmut Schaider

“…This was confirmed in a 3D spheroid model. The apoptosis‐inducing effect of magnolol was completely rescued by activating Akt suggesting a mechanism relying primarily on Akt signaling. …”
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Continuous Inhibition of Sonic Hedgehog Signaling Leads to Differentiation of Human-Induced Pluripotent Stem Cells into Functional Insulin-Producing β Cells by Song Lee, Jae Hyun Joo, Ju Yun Oh, Eun Ha Seo, Yang Hee Kim, Eunsung Jun, In Kyong Shim, Song Cheol Kim

“…Images of the 2D culture showed that IPCs formed spheroids from day 5 and continuously secreted insulin. …”
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Endothelial tip-cell position, filopodia formation and biomechanics require BMPR2 expression and signaling by Christian Hiepen, Mounir Benamar, Jorge Barrasa-Fano, Mar Condor, Mustafa Ilhan, Juliane Münch, Nurcan Hastar, Yannic Kerkhoff, Gregory S. Harms, Thorsten Mielke, Benjamin Koenig, Stephan Block, Oliver Rocks, Salim Abdelilah-Seyfried, Hans Van Oosterwyck, Petra Knaus

“…Even more strikingly, in mosaic spheroids, BMPR2-deficient ECs failed to acquire tip-cell positions. …”
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Antibody-based delivery of interleukin-2 modulates the immunosuppressive tumor microenvironment and achieves cure in pancreatic ductal adenocarcinoma syngeneic mice by Carmine Carbone, Roberto De Luca, Emanuele Puca, Antonio Agostini, Alessia Caggiano, Lorenzo Priori, Annachiara Esposito, Serena Ascrizzi, Geny Piro, Lisa Salvatore, Francesco De Sanctis, Stefano Ugel, Vincenzo Corbo, Dario Neri, Giampaolo Tortora

“…To assess the efficacy of this immunocytokine we developed an ex-vivo immune-spheroid interaction platform derived from murine 3D pancreatic cultures, and telomerase reverse transcriptase (TERT) specific T-lymphocytes. …”
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Testicular biometrics of Murrah buffaloes in different age groups by Erly L. Carrascal-Triana, Jorge Mejía Lúquez, Divier Agudelo Gómez, Edison Julián Ramírez Toro

“…The TV was determined using the equation of the spheroid prolate and represented the average of both testes. …”
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Wilms tumor primary cultures capture phenotypic heterogeneity and facilitate preclinical screening by Lisa Götz, Jenny Wegert, Alireza Paikari, Silke Appenzeller, Sabrina Bausenwein, Christian Vokuhl, Taryn D. Treger, Jarno Drost, Christin Linderkamp, Dominik T. Schneider, Karen Ernestus, Steven W. Warman, Jörg Fuchs, Nils Welter, Norbert Graf, Sam Behjati, Rhoikos Furtwängler, Manfred Gessler

“…Adherent, spheroid and organoid cultures are also clearly distinguishable by their transcriptome. …”
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Platelet-derived extracellular vesicles induced through different activation pathways drive melanoma progression by functional and transcriptional changes by Zeynep Tavukcuoglu, Umar Butt, Alessandra V. S. Faria, Johannes Oesterreicher, Wolfgang Holnthoner, Saara Laitinen, Mari Palviainen, Pia R-M Siljander

“…Differentially expressed genes and enriched signaling pathways in the PEV-treated spheroids were analyzed at 6 h and 24 h by RNA sequencing. …”
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Novel banana lectin CAR-T cells to target pancreatic tumors and tumor-associated stroma by Malcolm K Brenner, Challice Bonifant, Norihiro Watanabe, Mary K McKenna, Daniel Brenner, Ada Ozcan, Maureen Legendre, Dafydd G Thomas, Christopher Ashwood, Richard D Cummings, David M Markovitz

“…In three-dimensional (3D) spheroid models, we measured H84T CAR T-cell disruption of PSC architecture, and T-cell infiltration by live imaging. …”
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Generation of Insulin-Producing Cells from Canine Adipose Tissue-Derived Mesenchymal Stem Cells by Takahiro Teshima, Keiji Okamoto, Kazuho Dairaku, Tomokazu Nagashima, Masaki Michishita, Ryohei Suzuki, Hirotaka Matsumoto, Hidekazu Koyama

“…Culturing AT-MSCs with the differentiation protocol under a two-dimensional culture system did not produce IPCs. However, spheroid-like small clusters consisting of canine AT-MSCs and human recombinant peptide μ-pieces developed under a three-dimensional (3D) culture system were successfully differentiated into IPCs. …”
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Co-isolation of human donor eye cells and development of oncogene-mutated melanocytes to study uveal melanoma by Yunxi Chen, Eva Jin, Mohamed Abdouh, Éric Bonneil, Daniel Alexander Jimenez Cruz, Thupten Tsering, Qianqian Zhou, Aurélie Fuentes-Rodriguez, Alexandra Bartolomucci, Alicia Goyeneche, Solange Landreville, Miguel N. Burnier, Julia V. Burnier

“…Particularly, Matrigel™ coating induced spheroid growth under certain coating thickness and cell seeding density but did not improve NCM metabolic activity. …”
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Preclinical Efficacy and Proteomic Prediction of Molecular Targets for s-cal14.1b and s-cal14.2b Conotoxins with Antitumor Capacity in Xenografts of Malignant Pleural Mesothelioma by Angélica Luna-Nophal, Fernando Díaz-Castillo, Vanessa Izquierdo-Sánchez, Jesús B. Velázquez-Fernández, Mario Orozco-Morales, Luis Lara-Mejía, Johana Bernáldez-Sarabia, Noemí Sánchez-Campos, Oscar Arrieta, José Díaz-Chávez, Jorge-Ismael Castañeda-Sánchez, Alexei-Fedorovish Licea-Navarro, Saé Muñiz-Hernández

“…However, only s-cal14.1b modified spheroid growth. Approximately 600 proteins exhibited important differential expression, which was more heterogeneous in H2452 vs MSTO-211H spheroids. …”
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Sodium valproate enhances efficacy of NKG2D CAR-T cells against glioblastoma by Junchen Liu, Junchen Liu, Kun Dai, Kun Dai, Muhammad Auwal Saliu, Muhammad Auwal Saliu, Mansur Dabai Salisu, Mansur Dabai Salisu, Jiangyu Gan, Lukman Olalekan Afolabi, Dehong Yan, Guizhong Zhang, Maoxuan Liu, Maoxuan Liu, Xiaochun Wan, Xiaochun Wan

“…VPA treatment enhanced the susceptibility of glioblastoma cells to NKG2D CAR-T mediated cytotoxicity in both 2D monolayer and 3D tumor spheroid models in vitro. Moreover, VPA-treated glioblastoma cells stimulated CAR-T cells to produce higher levels of inflammatory cytokines (IL-2, IFN-γ, and IL-6). …”
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Duodenal Organoids From Metabolic Dysfunction-Associated Steatohepatitis Patients Exhibit Absorptive and Barrier Alterations by Alia Hadefi, Morgane Leprovots, Gilles Dinsart, Maryam Marefati, Marjorie Vermeersch, Daniel Monteyne, David Pérez-Morga, Anne Lefort, Frédérick Libert, Laurine Verset, Claire Liefferinckx, Christophe Moreno, Jacques Devière, Eric Trépo, Marie-Isabelle Garcia

“…Despite global heterogeneity in growth patterns, MDEOs frequently exhibited cystic spheroid morphology. MDEOs displayed altered digestive potential associated with reduced mature absorptive cell fate, but they retained their lipid metabolic capacity, possibly mediated by lipid oxidation in stem/progenitor cells. …”
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Transcriptional landscape and predictive potential of long noncoding RNAs in peritoneal recurrence of gastric cancer by Xiao-Xia Cai, Guo-Ming Chen, Zi-Qi Zheng, Yi-Xin Yin, Shuang Wang, Li Qiao, Xiao-Jiang Chen, Bai-Wei Zhao, Jin-Ling Duan, Cheng-Cai Liang, Ruo-Peng Zhang, Cheng-Zhi Wei, Fei-Yang Zhang, Bo-Wen Huang, Ze-Xian Liu, Zhi-Wei Zhou, Dan Xie, Mu-Yan Cai, Shu-Qiang Yuan, Yuan-Fang Li, Run-Cong Nie

“…The role of lncRNAs in the PM of GC was then examined through wound healing, Transwell, 3D multicellular tumor spheroid invasion, and peritoneal cavity xenograft tumorigenicity assays in mice. …”
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Dimorphos’s Material Properties and Estimates of Crater Size from the DART Impact by Angela M. Stickle, Kathryn M. Kumamoto, Dawn M. Graninger, Mallory E. DeCoster, Wendy K. Caldwell, Jason M. Pearl, J. Michael Owen, Olivier Barnouin, Gareth S. Collins, R. Terik Daly, Isabel Herreros, Jens Ormö, Jessica Sunshine, Carolyn M. Ernst, Toshi Hirabayashi, Simone Marchi, Laura Parro, Harrison Agrusa, Megan Bruck Syal, Nancy L. Chabot, Andy F. Cheng, Thomas M. Davison, Elisabetta Dotto, Eugene G. Fahenstock, Fabio Ferrari, Martin Jutzi, Alice Lucchetti, Robert Luther, Nilanjan Mitra, Maurizio Pajola, Sabina Raducan, KT Ramesh, Andrew S. Rivkin, Alessandro Rossi, Paul Sánchez, Stephen R. Schwartz, Stefania Soldini, Jordan K. Steckloff, Filippo Tusberti, Kai Wünnemann, Yun Zhang

“…Images from DART’s DRACO camera revealed Dimorphos to be an oblate spheroid covered in boulders of varying sizes and shapes. …”
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