Surface functionalization of microscaffolds produced by high-resolution 3D printing: A new layer of freedom by Oliver Kopinski-Grünwald, Stephan Schandl, Jegor Gusev, Ourania Evangelia Chamalaki, Aleksandr Ovsianikov

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The Effect of Heat Treatment on the Microstructure and Properties of H13ESR Large Mould Steel Forging Round Bar by Li　Jin, Zhou　Xu, Su　Chao, Yan　Lei

Subjects: “…online warter cooling after forged； offine solid solution； water and air cooling alternating quenching； spheroidization annealing； liquid precipitated carbides； metallographic structure…”
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Hematopoietic Stem and Progenitor Cell Expansion in Contact with Mesenchymal Stromal Cells in a Hanging Drop Model Uncovers Disadvantages of 3D Culture by Olga Schmal, Jan Seifert, Tilman E. Schäffer, Christina B. Walter, Wilhelm K. Aicher, Gerd Klein

“…When seeded as a mixed cell suspension, MSCs segregated into tight spheroids. Despite the high expression of niche-specific extracellular matrix components by spheroid-forming MSCs, HSPCs did not migrate into the spheroids in the initial phase of coculture, indicating strong homotypic interactions of MSCs. …”
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Tending to the Facial Surfaces of a Mathematical Biology Head-Scratcher: Why Does the Head of the Sea Turtle <i>Natator depressus</i> Resemble a Convex Zygomorphic Dodecahedron? by David A. Becker

“…A segmented, biomorphic, convex polyhedral model to address cephalic topology is thus presented stemming from solid geometry, anatomical observations, and a recently computed densest local packing arrangement of fifteen slightly oblate spheroids in which fourteen oblate spheroids surround a central such spheroid. …”
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Unveiling immune cell response disparities in human primary cancer-associated fibroblasts between two- and three-dimensional cultures. by Jian-Ping Yang, Nikhil Nitin Kulkarni, Masashi Yamaji, Tsubasa Shiraishi, Thang Pham, Han Do, Nicole Aiello, Michael Shaw, Toshihiro Nakamura, Akiko Abiru, Narender R Gavva, Shane R Horman

“…Remarkably, when cultured in 2D, CAFs exhibited a myofibroblast (myCAF) subtype, whereas in 3D tumor spheroid cultures, CAFs displayed a more inflammatory (iCAF) pathological state. …”
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The Effect of FOXA3 Overexpression on Hepatocyte Differentiation and Liver Regeneration in a Fah cKO Mouse ModelSummary by Shao Li, Chupeng Ou, Jiajun Zhang, Min Zeng, Kangyan Liang, Qing Peng, Yi Gao

“…Results: HepaRG cells that overexpressed FOXA3 had hepatocyte-specific functions. RF3 spheroids expressed liver markers following gene and protein expression analysis. …”
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In-depth imaging of in-vitro tissue models using electrical impedance tomography by Jian Deng, Babu Linkoon P Meenaketan, Saeedeh Ebrahimi Takalloo, Yoke Chin Cha, Tom Torfs, Dries Braeken, Jan De Beenhouwer, Jan Sijbers

“…The performance of the proposed system is validated through simulations and experimental evaluations, demonstrating its significant potential for advancing biological research, particularly in applications such as organoid and spheroid culturing. …”
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An Efficient Computation of Effective Ground Range Using an Oblate Earth Model by Dalal A. Maturi, Malik Zaka Ullah, Shahid Ahmad, Fayyaz Ahmad

“…The spherical Earth model does not provide good approximation of distance between two locations on the surface of Earth. We used oblate spheroid Earth model because it provides better approximations. …”
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Reduction of elevated Gli3 does not alter the progression of autosomal recessive polycystic kidney disease by Lauren G. Russell, Maria Kolatsi‐Joannou, Laura Wilson, Jennifer C. Chandler, Nuria Perretta Tejedor, Georgie Stagg, Karen L. Price, Christopher J. Rowan, Tessa Crompton, Norman D. Rosenblum, Paul J. D. Winyard, David A. Long

“…We utilized Cpk mice which phenocopy ARPKD and generated a PKHD1‐mutant spheroid model in human collecting ducts. Significantly elevated levels of the Hh transcriptional effector Gli3 were found in Cpk mice, a finding replicated in PKHD1‐mutant spheroids. …”
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Advancing Radiobiology: Investigating the Effects of Photon, Proton, and Carbon-Ion Irradiation on PANC-1 Cells in 2D and 3D Tumor Models by Alexandra Charalampopoulou, Amelia Barcellini, Giuseppe Magro, Anna Bellini, Sara Sevan Borgna, Giorgia Fulgini, Giovanni Battista Ivaldi, Alessio Mereghetti, Ester Orlandi, Marco Giuseppe Pullia, Simone Savazzi, Paola Tabarelli De Fatis, Gaia Volpi, Angelica Facoetti

“…<b>Materials and Methods:</b> This study investigates the survival of pancreatic cancer cells in both 2D and spheroids, a 3D model, following photon, proton, and carbon-ion irradiation by means of clonogenic, MTT, spheroid growth, and vitality assays. …”
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Functionality of endothelial cells differentiated from porcine epiblast stem cells, bone marrow-derived mesenchymal stem cells and adipose-derived mesenchymal stem cells by Yeon-Ji Lee, In-Won Lee, Tae-Suk Kim, Bo-Gyeong Seo, Sang-Ki Baek, Cheol Hwangbo, Joon-Hee Lee

“…Evaluation of ECs differentiated from these stem cells was treated with capillary-like structure formation and three-dimensional spheroid sprouting assay. Results: Porcine pEpiSCs, pBM-MSCs and pA-MSCs showed similar expression of pluripotency-related genes (OCT-3/4. …”
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Dynamics around small irregularly shaped objects modeled as a mass dipole by Ahmed A. Abozaid, M. Radwan, A. H. Ibrahim, A. Bakry

“…The dipole consists of two connected masses, one is spherical and the other is an oblate spheroid. The gravitational potential of the elongated body is determined by four independent parameters. …”
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Effect on Multipotency and Phenotypic Transition of Unrestricted Somatic Stem Cells from Human Umbilical Cord Blood after Treatment with Epigenetic Agents by Foued Ghanjati, Simeon Santourlidis

“…This treatment leads to a new stable, spheroid-forming cell type which we have named SpheUSSC. …”
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Real-Time Analysis of Endogenous Wnt Signalling in 3D Mesenchymal Stromal Cells by Fatima Saleh, Alice Carstairs, S. Leah Etheridge, Paul Genever

“…Here, we used mesenchymal stromal cell (MSC) EGFP reporter lines responsive to Wnt pathway activation in a 3D spheroid culture system to mimic better the in vivo environment. …”
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Determination of Appropriate Thresholding Method in Segmentation Stage in Detecting Breast Cancer Cells by Adem Polat, Cihat Ediz Akbaba

“…Ob-serving an entire tumor spheroid that has the size of several centimeters in size in a single square image with a microscope is not possible, but with mini-Opto tomography it is possible. …”
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Investigating the sigma formation in homogenization treatment of the 309L stainless cast billet by Hung-Yang Chu, Min-Chang Wu, Ren-Kae Shiue, Sheng-Yuan Cheng

“…The homogenization of the cast 309L at 1240 °C for 2h can slightly spheroidize the morphology of δ ferrite. Further increasing the homogenization time to 6 h demonstrates the best spheroid effect of the δ ferrite and sigma in the cast 309L specimen. …”
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Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems by Vitale Miceli, Mariangela Pampalone, Serena Vella, Anna Paola Carreca, Giandomenico Amico, Pier Giulio Conaldi

“…In our study, we show that a three-dimensional (3D) culture method is effective to induce MSC spheroid formation, to maintain the multipotency and to improve the paracrine activity of a specific population of human amnion-derived MSCs (hAMSCs). …”
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A Benzochalcone Derivative, (E)-1-(2-hydroxy-6-methoxyphenyl)-3-(naphthalen-2-yl)prop-2-en-1-one (DK-512), Inhibits Tumor Invasion through Inhibition of the TNFα-Induced NF-κB/MMP-... by Soon Young Shin, Chang Gun Kim, Seunghyun Ahn, You Jung Jung, Dongsoo Koh, Young Han Lee, Yoongho Lim

“…We found that DK-512 strongly inhibited invasion of metastatic MDA-MB-231 breast cancer cells as revealed by a three-dimensional spheroid culture system. Tumor invasion and metastasis require disruption of the extracellular matrix. …”
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Optimized scaffold-free human 3D adipose tissue organoid culture for obesity and disease modeling by Rafael Dariolli, Raphael Nir, Tova Mushlam, Glauco R. Souza, Stephen R. Farmer, Miguel L Batista, Jr.

“…As adipocytes maturate and accumulate fat, they develop organotypic characteristics, increasing the buoyancy effect, which causes the organoids to oscillate, complicating culture manipulation and rendering multiple handling steps difficult.Due to these challenges, most adipose spheroid and organoid models are scaffold-based, despite many cell types' ability to secrete extracellular matrix (ECM) components and self-assemble into aggregates. …”
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Mebendazole Exerts Anticancer Activity in Ovarian Cancer Cell Lines via Novel Girdin-Mediated AKT/IKKα/β/NF-κB Signaling Axis by Rahul Gupta, Dipanjan Roy, Arijit Ghosh, Yasmin Begum, Dipanjan Ghosh, Snehasikta Swarnakar

“…Additionally, MBZ significantly impedes migration, spheroid invasion, colony formation, and stemness. …”
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