DETECTION OF CAMPYLOBACTER SPP. WITH REAL-TIME POLYMERASE CHAIN REACTION by G. S. Skitovich, K. V. Serova, N. B. Shadrova, O. V. Pruntova

Subjects: Get full text

Use of real-time polymerase chain reaction for investigation of Senecavirus infection occurrence in Russia by M. V. Timanov, A. M. Timina, M. V. Biryuchenkova

Get full text

Real-Time Polymerase Chain Reaction for Microbiological Diagnosis of Parapneumonic Effusions in Canadian Children by Jeffrey M Pernica, Ioana Moldovan, Francis Chan, Robert Slinger

Get full text

Introduction of Digital Polymerase Chain Reaction: Comparison with Real-time Polymerase Chain Reaction by Dongsup LEE

Get full text

Prevalence of chlamydophila psittaci infection in pigeons and paraquets by a real time polymerase chain reaction by Nevin Tuzcu, Hasan Huseyin Hadimli, Aysegul Ilban

Get full text

Evaluation of Amplification Targets for the Specific Detection of Bordetella pertussis Using Real-Time Polymerase Chain Reaction by Mohammad Rubayet Hasan, Rusung Tan, Ghada N Al-Rawahi, Eva Thomas, Peter Tilley

Get full text

DNA-based detection of Rat in the meatballs product using a real-time polymerase chain reaction method by Etin Diah Permanasari, Hadi Sunaryo, Adia Putra Wirman, Nuriza Rahmadini, Savira Yustinah Aggasy, Nurul Azmah Nikmatullah

“…This research aimed to detect rat meat contamination in meatball samples from the Indonesian local market using Real-time Polymerase Chain Reaction (RT-PCR). The RT-PCR amplification involved an initial denaturation step at 95°C for 3 minutes, followed by denaturation at 95°C for 15 seconds, and annealing/extension at 60°C for 1 minute. …”
Get full text

Comparison of Real-time Polymerase Chain Reaction and Culture for Targeting Pathogens in Pediatric Severe Community-Acquired Pneumonia by Khai Quang Tran, Van Hung Pham, Chau Minh Vo, Quan Minh Pham, Phuong Minh Nguyen

“…Objective: This study aims to determine the frequency of pathogen detection by real-time polymerase chain reaction (PCR), the frequency of pathogen isolation by culture; and compare the value of real-time PCR and culture of nasopharyngeal aspiration samples in patients with severe community-acquired pneumonia (sCAP). …”
Get full text

Rapid detection of Vibrio alginolyticus in seafood using the flgL gene and real-time polymerase chain reaction by Muktiningsih Nurjayadi, Gladys Indira Putri, Jefferson Lynford Declan, Ismaya Krisdawati, Dandy Akbar Juliansyah, Maharanianska Azzahra, Irvan Maulana, Irma Ratna Kartika, Fera Kurniadewi, Tiara Fahriza, Adinda Myra Amalia Putri, Ayu Berkahingrum, Atikah Nur Rahmawati, Rosita Gio Anggraeni, Dalia Sukmawati, Sri Rahayu, Vira Saamia, I Made Wiranatha, Bassam Abomoelak, Hesham Ali El-Enshasy

Subjects: Get full text

Differential Diagnosis of Entamoeba spp. in Clinical Stool Samples Using SYBR Green Real-Time Polymerase Chain Reaction by Thiago dos Santos Gomes, Mariana Coimbra Garcia, Flavia de Souza Cunha, Heloisa Werneck de Macedo, José Mauro Peralta, Regina Helena Saramago Peralta

Get full text

Investigation of IDO1 and TDO2 expression in breast tumors by immunohistochemistry and real-time polymerase chain reaction methods by Gülden Yıldız, Hüseyin Büyükbayram, Ulaş Alabalik, Ayşe Nur Keleş

Subjects: Get full text

Linear Methods for Analysis and Quality Control of Relative Expression Ratios from Quantitative Real-Time Polymerase Chain Reaction Experiments by Robert B. Page, Arnold J. Stromberg

“…Relative expression quantitative real-time polymerase chain reaction (RT-qPCR) experiments are a common means of estimating transcript abundances across biological groups and experimental treatments. …”
Get full text

Development of a novel strategy to reduce diagnostic errors in real-time polymerase chain reaction using probe-based techniques by Hyoung Jun Kim, Morten Schiøtt, Niels Jørgen Olesen, Euna Choi, Bok Kyung Ku, Kyoung Ki Lee, Hye Young Jeong, Ilseob Lee, Seong Mok Kim, Miyoung Cho, Young Chul Kim

Get full text

Identification of Common Bacterial Pathogens Causing Meningitis in Culture-Negative Cerebrospinal Fluid Samples Using Real-Time Polymerase Chain Reaction by Walaa Shawky Khater, Safia Hamed Elabd

“…Forty culture-negative CSF samples from suspected cases of bacterial meningitis were examined by real-time polymerase chain reaction (real-time PCR) for the presence of lytA, bexA, and ctrA genes specific for Streptococcus pneumoniae, Haemophilus influenzae, and Neisseria meningitidis, respectively. …”
Get full text

Determination of Real-Time Polymerase Chain Reaction Uncertainty of Measurement Using Replicate Analysis and a Graphical User Interface with Fieller’s Theorem by James Ian Stuart, Johan Delport, Robert Lannigan, George Zahariadis

“…BACKGROUND: Disease monitoring of viruses using real-time polymerase chain reaction (PCR) requires knowledge of the precision of the test to determine what constitutes a significant change. …”
Get full text

Melting curve analyses in the quantitative real-time polymerase chain reaction of methylated/non-methylated DNA toward the detection of oral cancer using gargle fluid by Yusaku Noma, Hayate Sawayama, Kouta Yamashiro, Keitaro Nishi, Haruka Yoshinaga, Ryota Takaoka, Tsuyoshi Sugiura, Tomonori Sasahira, Tomofumi Hamada, Yasuo Suda, Tatsuo Okui

“…We therefore sought to develop a simple screening method for the diagnosis of oral cancer by conducting a melting curve analysis in a quantitative real-time polymerase chain reaction (qPCR). The assay was performed by a qPCR of DNA from the gargle fluid of 20 patients with oral cancer and 20 healthy control subjects in the training set. …”
Get full text

Evaluation of Protocols for DNA Extraction from Individual <i>Culex pipiens</i> to Assess Pyrethroid Resistance Using Genotyping Real-Time Polymerase Chain Reaction by Ilaria Congiu, Elisa Cugini, Daniele Smedile, Federico Romiti, Manuela Iurescia, Valentina Donati, Claudio De Liberato, Antonio Battisti

Get full text

Effect of Anthelmintic Treatment on the Agreement Between Real-Time Polymerase Chain Reaction (RT-PCR) and Kato–Katz Microscopic Technique in the Diagnosis of Soil-Transmitted Helminth Infections by Paul Alvyn Nguema-Moure, Jean Claude Dejon-Agobé, Roméo-Aimé Laclong Lontchi, Gédéon Prince Manouana, Moustapha Nzamba Maloum, Brice Meulah, Danny Carrel Manfoumbi Mabicka, Marguerite Emmanuelle Nzame Ngome, Peter Gottfried Kremsner, Ayôla Akim Adegnika

Get full text

Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo by Roger Wumba, Menotti Jean, Longo-Mbenza Benjamin, Mandina Madone, Kintoki Fabien, Zanga Josué, Sala Jean, Kendjo Eric, Guillo-Olczyk AC, Thellier Marc

Get full text

Pro-angiogenic effects of Guo Min decoction in a zebrafish model by Ping-Hsun Lu, Sheng-Fen Tung, Chi-Chung Wen, Ching-Yuan Huang, Jhih-Wei Huang, Chih-Hsin Chen, Yau-Hung Chen

Subjects: Get full text