内毒素对NRK52E细胞生长及细胞间缝隙连接功能的影响

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A Proteomics Analysis to Evaluate Cytotoxicity in NRK-52E Cells Caused by Unmodified Nano-Fe3O4 by Yi-Reng Lin, Chao-Jen Kuo, Hugo You-Hsien Lin, Chin-Jen Wu, Shih-Shin Liang

“…We synthesized unmodified Fe3O4 nanoparticles (NPs) with particles size from 10 nm to 100 nm. We cultured NRK-52E cell lines (rat, kidney) and treated with Fe3O4 NPs to investigate and evaluate the cytotoxicity of NPs for NRK-52E cells. …”
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白蛋白对近端肾小管上皮细胞增殖与凋亡的影响 by 孙良忠, 岳智慧, 陈述枚

“…【结果】高剂量dBSA超载未完全融合NRK52E细胞144h后细胞变得更密集。MTS比色结果发现，高剂量dBSA（5、10、30mg／mL）超载可明显诱导NRK52E细胞增殖，增殖活性分别为（0．700±0．045）A、（1．021±0．029）A和（1．273±0．173）A，较对照细胞（0．441±0．020）A显著升高，P均〈0．05。…”
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miR-29c Inhibits Renal Interstitial Fibrotic Proliferative Properties through PI3K-AKT Pathway by Weifeng Feng, Huijun Xie, Jiong Li, Xianxin Yan, Shiping Zhu, Shengyun Sun

“…This study revealed that miR-29c inhibited TGF-β1 expression in NRK-52E cell cultures. Overexpression of miR-29c significantly inhibits NRK-52E culture proliferation mediated by TGF-β1. miR-29c inhibited the expression of Col-1 and decreased PI3K/Akt phosphorylation. …”
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Rational Design for Improving the Thermostability of Saccharomyces cerevisiae Nicotinamide Riboside Kinase 1 by WANG Yao, SHEN Taisong, LI Sichen, SHI Hongling, YAO Lunguang, TANG Cunduo

“…In order to improve the thermal stability of nicotinamide riboside kinase 1 from Saccharomyces cerevisiae (ScNRK1), six single-point mutants of ScNRK1 were virtually designed using computer-aided technology, and their expression using site-directed mutagenesis and enzymatic characterization were carried out. …”
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环孢素A对肾小管上皮细胞MMP-2和 MMP-9表达和活性的影响

“…【目的】 观察环孢素A(CSA)对近端肾小管上皮细胞基质金属蛋白酶-2和-9(MMP-2 和 MMP-9)表达和活性的影响65377;【方法】 体外培养大鼠近端肾小管上皮细胞株NRK52E细胞至完全融合时,分别给予不同浓度的CSA 0,0.42,0.84,4.2,8.4 μmoL/L,实验观察48 h和72 h65377;收集培养液,用明胶酶谱法检测MMP-2和MMP-9活性65377;提取总RNA,逆转录聚合酶链式反应(RT-PCR)检测MMP-2和MMP-9的mRNA水平65377; 【结果】 CSA刺激NRK52E细胞呈剂量依赖性抑制MMP-2活性和mRNA表达65377;与对照组(CSA 0 μmoL/L)比较,CSA0.42,0.84,4.2,8.4 μmoL/L的MMP-2活性分别下降为27%,24%,11%,9%;差异均有统计学意义,P < 0.0565377;CSA刺激NRK52E细胞增加MMP-9活性和mRNA表达65377;与对照组比较,CSA 4.2 μmoL/L 刺激48 h MMP-9活性上调为438%,72 h上调为237%,差异均有统计学意义,P < 0.0565377; 【结论】 CSA对肾小管上皮细胞MMP-2表达和活性呈剂量依赖性抑制,以及对MMP-9表达和活性的诱导作用可能与CSA所致肾小管间质损害相关65377;…”
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The Geopolitics of Nordic Noir by Dodds Klaus, Hochscherf Tobias

“…We look specifically at the dramas Okkupert [Occupied] (NRK, 2015–), Ørnen [The Eagle] (DR, 2004–2006), Nobel – fred for enhver pris [Nobel – Peace at any Cost] (NRK, 2016), and Kriger [Warrior] (Netflix, 2018–) as they explore potential threats to Scandinavian society and the Nordic welfare state through the distinct figure of the vigilante veteran. …”
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A Mechanistic Study on the Non-genotoxic Carcinogenicity of the Food Contaminant Semicarbazide by Daniel P. Fitzpatrick

“…A novel finding of the present study was where NRK cells were exposed to micromolar concentrations of semicarbazide, an inverse relationship between protein kinase C activity, and free radical concentration proportionally increases > 2-fold. …”
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MicroRNA-22 Promotes Renal Tubulointerstitial Fibrosis by Targeting PTEN and Suppressing Autophagy in Diabetic Nephropathy by Yingying Zhang, Siqi Zhao, Depei Wu, Xingmei Liu, Mingjun Shi, Yuanyuan Wang, Fan Zhang, Jing Ding, Ying Xiao, Bing Guo

“…Moreover, microRNA-22 (miR-22) was upregulated and associated with reduced expression of its target gene phosphatase and tensin homolog (PTEN) in both the kidneys of DN rats and high glucose-cultured NRK-52E cells. Intriguingly, induction of autophagy by rapamycin antagonized high glucose-induced collagen IV (Col IV) and α-SMA expression. …”
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Non-lethal sonodynamic therapy mitigates hypertensive renal fibrosis through the PI3K/AKT/mTORC1-autophagy pathway by DanDan Liu, Hui Wang, Jialong Li, Siqi Sheng, Shu Wang, Ye Tian

“…In vitro. (1) The expression of α-SMA, collagen I and vimentin were increased significantly in TGF-β1-induced NRK-52E cells. (2) The increase of autophagosomes was observed in TGF-β1-induced NRK-52E cells after NL-SDT. …”
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What Methods Software Teams Prefer When Testing Web Accessibility by Aleksander Bai, Viktoria Stray, Heidi Mork

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Evaluation of the Cytotoxic Effects of CAM Therapies: An In Vitro Study in Normal Kidney Cell Lines by Shagun Arora, Chanderdeep Tandon, Simran Tandon

“…The parameters chosen for the study were based on antiproliferative and cytotoxic effects on normal, kidney epithelial cells (NRK-52E). The MTT assay, colony formation assay, DNA fragmentation, and differential staining using AO/EB, following treatment with either 5-FU or CAM therapies, were performed. …”
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RNAi干扰WT1和Pax2表达对逆转肾小管上皮间充质转化的作用

“…【目的】 探讨抑制肾小管上皮间充质转化(EMT)过程中胚胎发育关键基因WT1和Pax2的表达对EMT的逆转作用【方法】 采用RNAi技术分别抑制WT1和Pax2分别构建pshRNA-WT1和pshRNA-Pax2表达载体,采用脂质体转染技术,使质粒瞬时转染NEK52E细胞后用10 ng/mL IL-1α刺激细胞,分别提取不同时间点细胞的RNA和蛋白质,采用RT-PCR和Western blot检测细胞WT1Pax2Snail上皮细胞标志E-cadherin和间充质标志α-SMA的mRNA和蛋白质的表达,并观察NRK52E细胞的形态【结果】构建的pshRNA-WT1和pshRNA-Pax2表达载体可在转染细胞内发挥RNAi作用抑制WT1和Pax2基因的表达,抑制率分别为80.4 %和82.7 %分别抑制WT1和Pax2基因后EMT过程受阻,α-SMA和Snail的表达显著减少,E-cadherin的表达无显著性变化,细胞形态未发生明显的成纤维化样改变【结论】 WT1和Pax2基因是EMT中的关键基因,分别抑制胚胎发育关键基因WT1和Pax2均可使EMT受阻阻断WT1和Pax2的表达有望中止EMT及肾纤维化的发生…”
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不同固定剂在激光共聚焦荧光技术中的效果评价

“…【目的】 探讨3种不同的固定剂在激光共聚焦免疫荧光技术中对信号表达的影响。 【方法】 对培养的NRK-52E细胞分成胞浆,胞膜及胞核抗原3组,每组用4 g/L PFA,甲醇及丙酮分别固定后,完成免疫荧光实验;原代滑膜成纤维样细胞分两组用4 g/L PFA 固定后分别用TritonX-100 和预冷的甲醇进行打孔,完成免疫荧光实验之后,使用Confocal显微镜在条件同等的情况下观察并拍摄荧光图像,以证实不同的固定剂对信号表达的影响。…”
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NADPH氧化酶在TGF-β1诱导大鼠小管上皮细胞 表达炎症因子中的作用 by 张海燕, 姜宗培, 常洁, 李晓艳, 朱恒梅, 余学清

“…【目的】 观察转化生长因子β1(TGF-β1)对大鼠肾小管上皮细胞单核细胞趋化因子-1(MCP-1)及白细胞介素-6 (IL-6) 表达的影响,并探讨NADPH氧化酶在其中的作用?【方法】 以大鼠肾小管上皮细胞株(NRK-52E)为研究对象,采用TGF-β1(10 ng/mL)刺激0 h, 2 h, 4 h, 8 h, 12 h, 24 h分别收取RNA;刺激0 h, 12 h, 24 h,48 h, 72 h分别收取细胞上清液;部分实验中培养的细胞在刺激前用NADPH氧化酶抑制剂DPI (0.1, 1, 5, 10 μmol/L)预处理1 h, 然后含10 ng的TGF-β1无血清DMEM/F12培养液分别培养12 h (收集RNA)和24 h (收集细胞上清液)?…”
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Rare variant associations with birth weight identify genes involved in adipose tissue regulation, placental function and insulin-like growth factor signalling by Katherine A. Kentistou, Brandon E. M. Lim, Lena R. Kaisinger, Valgerdur Steinthorsdottir, Luke N. Sharp, Kashyap A. Patel, Vinicius Tragante, Gareth Hawkes, Eugene J. Gardner, Thorhildur Olafsdottir, Andrew R. Wood, Yajie Zhao, Gudmar Thorleifsson, Felix R. Day, Susan E. Ozanne, Andrew T. Hattersley, Stephen O’Rahilly, Kari Stefansson, Ken K. Ong, Robin N. Beaumont, John R. B. Perry, Rachel M. Freathy

“…NOS3 (fetal and maternal-acting), NRK (fetal), and ADAMTS8 (maternal-acting) have been implicated in placental function and hypertension. …”
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