Protective Mechanism of Blood-brain Barrier of Cerebral Hemorrhage Model Rats with Different Acupuncture Manipulation Duration by Xiaozhuo SUN, Zhuolin YANG, Zhihong MENG

“…Five days later, the expressions of Occludin, Claudin-5 and JAM-1 immunofluorescence positive cells in the cerebral hematoma area of rats under light microscope(×400)were compared.Results:(1)Expression of Occludin immunofluorescence positive cells:Compared with the sham-operated group, the Occludin expression in the model, acupuncture 5s and 60s groups decreased significantly(<italic>P</italic>&lt;0.05). …”
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Protocol for assessing immune-target cell interactions using a single-cell cytotoxicity assay by Zhihao Wei, Konglan Lin, Min Huang, Shicheng Su, Yiwen Lu

“…Here, we present a protocol for continuous observation of natural killer (NK) cell-mediated cytotoxicity with microwell arrays using an automated microscope. We describe steps for isolating and labeling primary NK cells, loading cells onto microwell arrays, monitoring target wells, and image analysis. …”
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CuAl-LDH Modified with Filamentous Macroalgae for Anionic Dyes Removal: A Study on Selectivity, Adsorption Efficiency, and Regeneration by Alfan Wijaya, Laila Hanum, Elda Melwita, Aldes Lesbani

“…The structure and morphology of the prepared CuAl-LDH/SA were identified by X-ray Diffraction (XRD), Fourier Transform Infra Red (FT-IR), (Brunauer-Emmett-Teller) BET surface area, Thermogravimetry / Differential Thermal Analyzer (TG/DTA), and Scanning Electron Microscope (SEM). For the adsorption process, the effects of initial pH, contact time, initial concentration, temperature, adsorption selectivity, and adsorbent regeneration, as well as kinetics, isotherms, and thermodynamics were studied. …”
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Experimental Study on the Permeability of Weakly Cemented Rock under Different Stress States in Triaxial Compression Tests by Gangwei Fan, Mingwei Chen, Dongsheng Zhang, Zhen Wang, Shizhong Zhang, Chengguo Zhang, Qizhen Li, Bobo Cao

“…Analyses based on scanning electron microscope (SEM) observations and mineral composition indicate that the samples are rich in clay minerals such as montmorillonite and kaolin, whose inherent properties of hydroexpansiveness and hydrosliming can be considered the dominant factors contributing to the seepage properties of weakly cemented rock with low permeability.…”
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Process Mineralogy and Occurrence State of Lithium of Spodumene Tailings in Sichuan Province by Panpan WANG, Junfeng WU, Lin CHEN, Yanqing CHEN

“….), optical semi-quantitative analysis, chemical analysis, MLA automatic quantitative detection of minerals, LA-ICPMS in-situ analysis of mineral trace elements, and electron microscope energy spectrum analysis, X-ray powder crystal diffraction analysis and other work. …”
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Effects of Vacuum Processing Treatment on the Structure and Physical and Chemical Properties of Fresh Waxy Corn Starch by Ruihang XU, Libin ZHU, Dan ZHU, Wenyi WEI, Guangcai NIU, Chenyu ZHAO, Yufan ZHANG

“…The microstructure and physicochemical properties of fresh waxy corn starch after vacuum processing treatment were investigated by scanning electron microscope, X-ray diffractometer, Fourier infrared spectrometer, differential scanning calorimeter and rapid viscosity analyzer. …”
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Defluoridation of Aqueous Solution Using Thermally Activated Biosorbents Prepared from Adansonia digitata Fruit Pericarp by David Mihayo, Maheswara Rao Vegi, Said Ali Hamad Vuai

“…The scanning electron microscope and Fourier transform infrared spectrometry were used to determine morphological features and functional groups of biosorbents. …”
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Susceptibility of Sardinella lemuru to emerging marine microplastic pollution by J.D.H. Palermo, K.L. Labrador, J.D. Follante, A.B. Agmata, M.J.R. Pante, R.N. Rollon, L.T. David

“…The stomach contents of 600 sardines were examined visually under a microscope, stained with Rose Bengal, and tested with hot needle technique to identify ingested microplastics. …”
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Influence of surface texture on osteogenic differentiation of dental pulp stem cells: An in vitro study by Komal Rajpurohit, Vidya Dodwad, Avinash Kharat, Spoorthi Belludi, Pooja Pharne, Sarah Marium

“…The isolated cells were stained using 1% alizarin red stain to identify the number of stem cells per square centimeter area under the light microscope. Results: The osteogenic differentiation of both the materials was compared with standard control (MSCs treated with osteogenic differentiation media only). …”
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The Amphoteric Ion Exchange Membrane Based on CS/CMC for Tobacco-Protein Adsorption and Separation from Tobacco Extract by Jun Ling, Yixiao Li, Bo Zhou, Baokun Zhu, Xinru Zhang, Yonghong Wang, Tiandong Zhang, Wanying Feng

“…A scanning electron microscope was used to observe the morphology of CS/CMC blend membranes, in which a three-dimensional opening structure was formed, and no phase separation was discovered. …”
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Direct immunofluorescence (DIF) versus immunohistochemical (IHC) staining of complements and immunoglobulins (Ig) in pemphigus group by Deepika Rana, Nita Khurana, Shramana Mandal, Bijay Laxmi Sahoo

“…As DIF needs frozen sections, fluorescent tagged antibodies, UV light microscope for examination, and trained personnel, its non-availability makes a definitive diagnosis challenging. …”
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Tribological characteristics of Al–Mg–Si–Cu alloy against die steel under elevated temperatures in simulated hot extrusion environment by Zhiwen Liu, Ming Zhang, Ping Yan, Xiao Liu, Luoxing Li

“…The wear tracks and wear debris on disc samples were observed by means of ultradepth 3D microscopy, field-emission scanning microscope and energy dispersive spectroscopy. The change trends of wear depth, width and wear rate of aluminium disc with temperature and wear cycles were investigated. …”
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Experimental study of combined thermal flooding in improving heavy oil flowability by Yongqiang Tang, Haitao Wang, Qiaoyu Ge, Suobing Zhang, Zengmin Lun, Xia Zhou, Tao Ma

“…The emulsification was observed using a confocal laser scanning fluorescence microscope (CLSFM). Findings reveal that steam flooding can boost crude oil flowability by augmenting temperature, while a chemical system tends to produce low-viscosity oil-in-water emulsions, thus further aiding the flow of crude oil. …”
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The Protecting Effects and Mechanisms of Baicalin and Octreotide on Heart Injury in Rats with SAP by Zhang Xiping, Tian Hua, Chen Hanqing, Chen Li, Wang Zhiwei, Wang Keyi, Yan Wei, Li Yun, Li Qingyu, He Qing, Wang Fei

“…The pathological myocardial changes under light microscope were milder in treated groups than in model group. …”
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Physicochemical properties, structure, growth-promoting and antioxidant activities of Glycyrrhiza leaf polysaccharides modified by fermentation by Juan Du, Yuan Wang, Xia Li, Zhalaga, Na Liu, Wenwen Wang, Jingwei Qi, Xiaoli Fan, Xiaoping An

“…In parallel, in vitro antioxidant activities assays demonstrated that Glycyrrhiza leaf fermented for 36 h exhibited strong DPPH, hydroxyl radical scavenging, and reducing power. Scanning electron microscope and infrared spectroscopy analyses revealed differences in the surface morphology of unfermented and fermented Glycyrrhiza leaf, with both polysaccharides being acidic and containing pyranose rings and α-glycosidic bonds. …”
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Protocol for investigating astrocytic mitochondria in neurons of adult mice using two-photon microscopy by Jian Zhou, Fengling Du, Fan Zhang, Xianhui Zhang, Yuanyuan Wu, Lifang Zhang, Ping Liao, Tianqi Tu, Jianhua Peng, Tao Li, Yong Jiang

“…In this context, we present a protocol for capturing astrocytic mitochondria in neurons of adult mice using a two-photon microscope. We describe an approach for constructing a mouse model with combined labeling of astrocytic mitochondria and neurons. …”
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Characterization of Ti6Al4V powders produced by different methods for selective electron beam melting by Liu Z., Huang C., Gao C., Liu R., Chen J., Xiao Z.

“…Theywere characterized by particle size and shape analyzer, scanning electron microscope, and X-ray diffraction. The results show some benefits of using PA Ti6Al4V powder in selective electron beam melting. …”
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Optimization of Conditions for Expression of Dengue Serotype 2 EDIII Protein in <i>Escherichia coli</i> and Immune Responses of Adjuvant-Free EDIII Ferritin Nanoparticles Against D... by M.S.B.W.T.M. Nipuna Sudaraka Tennakoon, Kyoung-Ho Lee, Hye-Mi Lee, Jae-Yeon Park, Hyun-Jin Shin

“…Purified, properly refolded, aggregate-free EDIII and EDIII ferritin proteins were obtained, and ferritin nanoparticles were identified using an electron microscope. By analyzing the immunogenicity of mouse serum, EDIII ferritin generated significantly higher IgG responses and neutralizing activity than EDIII-immunized mice. …”
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Impact of chlorine dioxide and chlorhexidine mouthwashes on friction and surface roughness of orthodontic stainless steel wires: an in-vitro comparative study [version 2; peer revi... by Arun S Urala, Shivani Apte, Divya S

“…The remaining 4 specimens from each group were sent for surface morphology evaluation using an atomic force microscope. Results Friction resistance evaluation for the archwires revealed a mean friction of 0.011 ± 0.0056 in Group A, 0.015 ± 0.0052 in Group B, and 0.010 ± 0.0067 in Group C. …”
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MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wnt/β-Cateninsignaling Pathway by Xiaohong Wu, Yong Zhang, Junping Guo, Xun Yan, Li Shen, Jianping Zhou, Jun Zhao, Min Zhuang, Zhihong Cao

“…Lentiviral vector pGCSIL-shMAC30-GFP of the RNA interference (RNAi) of the MAC30 gene was transfected into gastric cancer BGC-823 cell line and the expression of lentivirus label protein GFP was observed via fluorescence microscope, while cell proliferation and apoptosis were determined with flow cytometry and MTT assay, respectively. …”
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