EFFECT OF PLATELET RICH FIBRIN AND MESENCHYMAL STEM CELL ON MYOBLAST AND FIBROBLASTS RATIO IN RABBIT MUSCLE DEFECT by Dwikora Novembri Utomo, Mohammad Zaim Chilmi, Angga Fiandana

Subjects: “…allogenic msc…”
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Intranasal delivery of hMSC-derived supernatant for treatment of ischemic stroke by inhibiting the pro-inflammatory polarization of neutrophils by Yixiang Jiang, Ning Wang, Jingyi Liu, Haoran Ren, Wenkang Jiang, Yanting Lei, Xidan Fu, Miao Hao, Xiujuan Lang, Yumei Liu, Xijun Liu, Rui Li, Hulun Li

“…The neuroprotective effects of hMSC-L and hMSC-M were assessed with TTC staining, behavioral tests and pathological staining. …”
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Proteomic profiling of iPSC and tissue-derived MSC secretomes reveal a global signature of inflammatory licensing by Margeaux Hodgson-Garms, Matthew J. Moore, Mikaël M. Martino, Kilian Kelly, Jessica E. Frith

“…The data and findings from this study will inform the optimum MSC source for particular applications and underpin further development of MSC therapies.…”
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The role of natural exosomes from SHED-MSC in immunoregulation of M0/M1 polarized macrophage cells by Ali Fallah, Abasalt Hosseinzadeh Colagar, Ayyoob Khosravi, Ayyoob Khosravi, Azadeh Mohammad-Hasani, Azadeh Mohammad-Hasani, Mohsen Saeidi, Mohsen Saeidi

“…This study focused on exosomes derived from stem cells of human exfoliated deciduous teeth (SHED-MSC-EXOs) and their role in managing the balance of immunoregulatory macromolecules that play a role in the underlying immunoregulatory mechanisms in THP-1-derived M0/M1 macrophage cells.MethodsFlow cytometry confirmed the expression of CD14, CD68, CD80, and CD86 markers in these macrophages. …”
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hUC-MSC extracellular vesicles protect against hypoxic-ischemic brain injury by promoting NLRP3 ubiquitination by Shanshan Xiao, Ying Lv, Xuejing Hou, Shuqiang Qu

“…This study explores how hUC-MSC-EVs protect neonatal rats from HIBD. hUC-MSC-EVs were isolated, characterized, and administered to neonatal rats subjected to HIBD. …”
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EFEKTIVITAS PUSAT INFORMASI KONSELING REMAJA (PIK-R); STUDI KUALITATIF DENGAN TEKNIK MOST SIGNIFICANT CHANGE (MSC) by Yoga Tri Nugroho, Rumita Ena Sari, Arnild Augina Meckarische

Subjects: “…most significant change (msc)…”
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Erratum to “Vitamin D Promotes MSC Osteogenic Differentiation Stimulating Cell Adhesion and αVβ3 Expression” by Francesca Posa, Adriana Di Benedetto, Elisabetta A. Cavalcanti-Adam, Graziana Colaianni, Chiara Porro, Teresa Trotta, Giacomina Brunetti, Lorenzo Lo Muzio, Maria Grano, Giorgio Mori

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Increased Haematopoietic Supportive Function of USSC from Umbilical Cord Blood Compared to CB MSC and Possible Role of DLK-1 by Simone Maria Kluth, Teja Falk Radke, Gesine Kögler

“…In contrast to stromal cells from the adult bone marrow (BM) or adipose tissue, cord blood (CB) multipotent stromal cells (MSC) are biologically younger. Since first being described by our group, delta like 1 homologue (DLK-1) was determined as a discriminating factor between the distinct cord blood-derived subpopulations: the unrestricted somatic stromal cells (USSC), which lack adipogenic differentiation capacity, and the BM MSC-like CB MSC. …”
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MSC-derived exosome ameliorates pulmonary fibrosis by modulating NOD 1/NLRP3-mediated epithelial-mesenchymal transition and inflammation by Wei Chen, Jie Peng, Xiangyi Tang, Shao Ouyang

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EVALUATION OF POLYMORPHONUCLEAR IMMUNE CELLS IN BIOCOMPATIBILITY TEST DFLP SPONGE CARTILAGE SCAFFOLD, ADIPOSE-DERIVED MSC AND SECRETOME WITH CARTILAGE INJECTION MODEL by Brilliant Citra Wirashada, Dwikora Novemberi Utomo, Lukas Widhiyanto

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WAC Facilitates Mitophagy‐mediated MSC Osteogenesis and New Bone Formation via Protecting PINK1 from Ubiquitination‐Dependent Degradation by Shuai Fan, Jinteng Li, Guan Zheng, Ziyue Ma, Xiaoshuai Peng, Zhongyu Xie, Wenjie Liu, Wenhui Yu, Jiajie Lin, Zepeng Su, Peitao Xu, Peng Wang, Yanfeng Wu, Huiyong Shen, Guiwen Ye

“…It plays a pivotal function in facilitating MSC osteogenesis and enhancing new bone formation both in vitro and in vivo. …”
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间充质干细胞及其培养上清与融冻产物对脐血CD34+ 细胞扩增及分化的作用

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重组腺病毒载体Ad-LMP-1的构建及其转染 MSC后成骨活性

“…【目的】 快速构建大鼠LIM矿化蛋白-1基因重组腺病毒载体,转染MSC,探讨LMP-1基因对MSC成骨分化和成骨活性的影响65377; 【方法】 从大鼠成骨细胞内提取总RNA,并设计LMP-1特异性引物进行RT-PCR,获取LMP-1基因后利用Invitrogen公司的TOPO定向克隆技术创建入门克隆pENTR/D-LMP-165377;转化TOP10细菌后,挑取阳性克隆摇菌提取质粒,入门克隆再与表达载体pAD/CMV/V5-DEST进行同源重组反应得到病毒载体pAD/CMV/V5-LMP-1,转化细菌后挑选阳性克隆摇菌提取重组病毒质粒,用PacI内切酶线性化后用转染293A细胞后得到重组腺病毒载体65377;以腺病毒为载体,将大鼠LMP-1基因体外转染于3代大鼠MSC细胞内,检测LMP-1基因在MSC细胞的表达,分别观察转染后实验组与对照组I型胶原65380;碱性磷酸酶65380;骨钙素表达变化以及骨钙结节的形成情况,评价LMP-1基因的成骨能力65377;【结果】 成功获取大鼠LMP-1基因,入门质粒与病毒表达质粒经过酶切鉴定以及测序验证65377;LMP-1基因能在MSC中高效表达,转然后MSC的I型胶原,碱性磷酸酶65380;骨钙素的表达明显增强,骨钙结节形成增多65377; 【结论】 利用Gateway 技术构建LMP-1重组腺病毒载体相对简单,可快捷获得的pAd-LMP-165377;pAd-LMP-1转染MSC后,LMP-1基因能促进MSC向成骨细胞分化,增强其成骨作用65377;…”
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The Effect of Pulsatile Flow on bMSC-Derived Endothelial-Like Cells in a Small-Sized Artificial Vessel Made by 3-Dimensional Bioprinting by Kang Woog Lee, Dae-Hyun Kim, Jun Hee Lee, Young-Nam Youn

“…This study is aimed at investigating the possibility of small-sized artificial vessels made by 3-dimensional bioprinting and the effect of pulsatile flow on bMSC-derived endothelial-like cells. Cells were harvested from rabbit bone marrow and primary cultured with or without growth factors. …”
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A Novel Secretory Vesicle from Deer Antlerogenic Mesenchymal Stem Cell-Conditioned Media (DaMSC-CM) Promotes Tissue Regeneration by Minkoo Seo, Jin-Chul Kim, Hyung-Ki Kim, Eun wook Choi, Suyeong Jeong, Ki Chang Nam, Mihue Jang

“…Herein, we report that conditioned media of mesenchymal stem cells (MSCs) isolated from deer antlers enhanced tissue regeneration through paracrine action via a combination of secreted growth factors and cytokines. Notably, DaMSC-conditioned media (DaMSC-CM) enhanced hair regeneration by activating the Wnt signaling pathway. …”
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MSC-Derived Extracellular Vesicle-Delivered L-PGDS Inhibit Gastric Cancer Progression by Suppressing Cancer Cell Stemness and STAT3 Phosphorylation by Benshuai You, Can Jin, Jiaxin Zhang, Min Xu, Wenrong Xu, Zixuan Sun, Hui Qian

“…Mesenchymal stem cell- (MSC-) derived extracellular vesicles (EVs) serving as delivery system have attracted extensive research interest, especially in cancer therapy. …”
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Intravenously Administered Human Umbilical Cord-Derived Mesenchymal Stem Cell (HucMSC) Improves Cardiac Performance following Infarction via Immune Modulation by Xiaoting Liang, Jing Liu, Mimi Li, Fang Lin, Rulin Zhuang, Qingshu Meng, Xiaoxue Ma, Yuanfeng Xin, Xin Gong, Zhiying He, Wei Han, Xiaohui Zhou, Zhongmin Liu

“…We hypothesized that intravenous (iv) administration of human umbilical cord-derived MSC (HucMSC) exerts systemic and local anti-inflammation effects, leading to improved heart function after MI. …”
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MSC-exosomes pretreated by Danshensu extracts pretreating to target the hsa-miR-27a-5p and STAT3-SHANK2 to enhanced antifibrotic therapy by Jiabin Liang, Jingxiu Zhao, Lin Yang, Qian Wang, Jing Liao, Jianhao Li, Weizhao Zhuang, Fanghong Li, Jinxian He, Yukuan Tang, Hanwei Chen, Chen Huang

“…The integration of Danshensu from traditional Chinese medicine into modern MSC exosome therapy represents a promising frontier in the development of novel treatments for fibrotic diseases.…”
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Strength and Rigidity Analysis for Transfer Case of Articulated Dump Trucks by Lu Kai, Yang Shuiyan, Guo Wanlong, Zhou Huanhui

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