Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation by Li Chen, Kaikai Shi, Weimin Qiu, Lars Aagaard, Moustapha Kassem

“…Methods. We infected hMSC with a CRISPR/Cas9 lentivirus system, with specific inducible dCas9-coupled transcription activator or repressor: dCas9-KRAB or dCas9-VP64, respectively, and established two hMSC lines (hMSC-CRISPRi and hMSC-CRISPRa) that can inhibit or activate gene expression, respectively. …”
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Molecular Mechanisms of Immunomodulation Properties of Mesenchymal Stromal Cells: A New Insight into the Role of ICAM-1 by Yury Rubtsov, Кirill Goryunov, Аndrey Romanov, Yulia Suzdaltseva, George Sharonov, Vsevolod Tkachuk

“…We examined the molecular mechanisms of MSC immunomodulatory function in mixed cultures of human adipose-derived MSC with lymphocytes. …”
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肾癌分泌IL-8激活ERK促进间充质干细胞迁移的机制

“…【目的】 拟探讨肾癌内IL-8的分泌水平及其对骨髓间充质干细胞(MSC)的调节机制,探讨其在归巢中的作用机制?【方法】 ELISA法检测肾癌患者血清以及肾癌细胞分泌的IL-8;免疫组织化学和RT-PCR检测MSC表面IL-8受体表达;迁移实验观察IL-8对MSC的迁移作用;Western blotting 法检测IL-8对MSC内ERK的激活作用?…”
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A framework for understanding the path to achieve high resilience of maritime supply chain by Xiuqian Chen, Liangyong Chu, Mengyao Wang, Qion Chen

“…The paper proposes a methodological framework based on the structural equation model (SEM), Necessary Condition Analysis (NCA), fuzzy set qualitative comparative analysis (fsQCA), and utilizes questionnaire data from the maritime industry in China to evaluate the resilience in MSC. The computational results indicate that: (1) The high resilience of MSC is the result of the synergistic interaction among multiple factors, and these factors boost MSC resilience through diverse paths; (2) There are four pathways for high MSC resilience: the ‘collaboration + carbon reduction + digitization’ path, the ‘early warning + collaboration’ path, the ‘agility + early warning’ path, and the ‘carbon reduction’ path; (3) Digitization level can play a catalytic role in increasing the resilience of MSC by helping other factors. …”
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MCP-1/CCR2通路参与骨髓基质细胞向脊髓全横断损伤区迁移的实验研究 by 丁鹏, 路钢, 杨智勇, 王进昆, 余化霖, 薛黎萍, 李园园, 冯忠堂, 林荣安

“…【目的】 探讨单核细胞趋化蛋白-1（MCP-1）及其受体CCR2在骨髓基质细胞（MSC）体内、外迁移中的作用。【方法】体外培养、纯化大鼠MSC，取第五代MSC行免疫荧光鉴定；免疫组化、RT-PCR检测纯化MSC表达CCR2情况；Boyden 小室法检测趋化因子MCP-1（5～500 ng/mL）对MSC的趋化迁移作用及其特异性。…”
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三种不同型别小鼠骨髓来源间质干细胞的生物学特性分析

“…【目的】间质干细胞(MSC)是一种间质来源的多潜能基质细胞，目前人和多种动物来源的MSC均已被成功分离鉴定然而由于小鼠骨髓MSC的分离相对人和其他物种更为困难，关于小鼠骨髓MSC的克隆分析结果也相对有限且结果不尽一致。…”
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Mesenchymal Stem Cell-Derived Neuron-Like Cell Transplantation Combined with Electroacupuncture Improves Synaptic Plasticity in Rats with Intracerebral Hemorrhage via mTOR/p70S6K S... by Guoqiang Yang, Jiayi Zhu, Guwen Zhan, Guangbi Fan, Li Deng, Huajun Tang, Xiaoqian Jiang, Bo Chen, Chaoxian Yang

“…Therefore, rat ICH models were established by injecting collagenase and heparin, and the animals were randomly divided into model control (MC), EA stimulation (EA), MSC-derived neuron-like cell transplantation (MSC-dNLCs), and MSC-dNLC transplantation combined with EA stimulation (MSC-dNLCs+EA) groups. …”
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Platelet-Rich Plasma Improves the Wound Healing Potential of Mesenchymal Stem Cells through Paracrine and Metabolism Alterations by Barbara Hersant, Mounia Sid-Ahmed, Laura Braud, Maud Jourdan, Yasmine Baba-Amer, Jean-Paul Meningaud, Anne-Marie Rodriguez

“…Mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) were separately tested in the attempt to regenerate the lost skin. …”
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Mesenchymal Stem Cell Therapy for Cardiac Inflammation: Immunomodulatory Properties and the Influence of Toll-Like Receptors by F. van den Akker, S. C. A. de Jager, J. P. G. Sluijter

“…Treatment with mesenchymal stem cells (MSC) upon MI positively affects cardiac function. …”
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间充质干细胞在致敏受者造血干细胞移植中的作用 by 许吕宏

“…【目的】 本实验拟研究骨髓间充质干细胞(MSC)在致敏受者造血干细胞移植中的作用?【方法】 体外分离BALB/c小鼠骨髓细胞,通过贴壁培养方法获得MSC,应用流式细胞仪检测细胞表面分子标记?…”
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人中期胚胎、新生儿脐血及成人骨髓间质干细胞基本生物学特性的比较

“…【目的】比较人中期胚胎脐血、新生儿脐血以及成人骨髓3种来源的间质干细胞(MSC)生物学特性,为临床治疗选择细胞来源提供实验依据。【方法】采用L-DMEM培养液分离培养3种来源MSC,流式细胞仪进行细胞表型、细胞周期、细胞凋亡分析,成骨、成脂诱导液分别诱导细胞分化,携带绿色荧光蛋白报告基因的重组腺病毒转染MSC,荧光显微镜和流式细胞仪检测3种细胞表达外源基因特性。…”
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Molecular Mechanisms Involved in Mesenchymal Stem Cell Migration to the Site of Acute Myocardial Infarction by Katarina Kollar, Matthew M. Cook, Kerry Atkinson, Gary Brooke

“…This review aims to summarise the current knowledge of MSC migratory mechanisms and looks to future methods of MSC manipulation prior to delivery in order to enhance MSC migration and engraftment.…”
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Ｌｕｃｉｆｅｒａｓｅ２／ｍＫａｔｅ２双报告基因对小鼠骨髓间充质干细胞的标记及活体光学成像研究

“…摘 要： 【目的】 Ｌｕｃｉｆｅｒａｓｅ２／ｍＫａｔｅ２双报告基因标记小鼠骨髓间充质干细胞（ｍＭＳＣ），并进行活体生物发光和荧光双模态光学成像研究。【方法】全骨髓细胞贴壁分离法提取ｍＭＳＣ，进行细胞表面标志物和多向分化潜能的鉴定；ＣＭＶ－Ｌｕｃｉｆｅｒａｓｅ２－ｍＫａｔｅ２慢病毒感染ｍＭＳＣ，根据细胞内远红荧光蛋白ｍＫａｔｅ２的表达水平评估转染效率并利用流式细胞仪进行纯化筛选；对筛选后的ｍＭＳＣ（ｍＭＳＣ－ＣＭＶ－Ｌｕｃ２－ｍＫａｔｅ２，ｍＫａｔｅ２＋＞９５％）进行活体生物发光和荧光双模态光学成像。 …”
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Molecular Profiles of Cell-to-Cell Variation in the Regenerative Potential of Mesenchymal Stromal Cells by Kim C. O’Connor

“…Cell-to-cell variation in the regenerative potential of mesenchymal stromal cells (MSCs) impedes the translation of MSC therapies into clinical practice. Cellular heterogeneity is ubiquitous across MSC cultures from different species and tissues. …”
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Cardiac Migration of Endogenous Mesenchymal Stromal Cells in Patients with Inflammatory Cardiomyopathy by Caroline Schmidt-Lucke, Felicitas Escher, Sophie Van Linthout, Uwe Kühl, Kapka Miteva, Jochen Ringe, Thomas Zobel, Heinz-Peter Schultheiss, Carsten Tschöpe

“…Mesenchymal stromal cells (MSC) have immunomodulatory features. The aim of this study was to investigate the migration and homing potential of endogenous circulating MSC in virus negative inflammatory cardiomyopathy (CMi). …”
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Effect of miR-144-3p-Targeted Regulation of PTEN on Proliferation, Apoptosis, and Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells under Stretch by Shiyong Ling, Xi Luo, Bo Lv, Hua Wang, Mengzhi Xie, Kai Huang, Jingchuan Sun

“…ALP staining showed normal activity in the MSC group and decreased ALP activity in the tension MSC group compared to the MSC group. …”
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骨髓间充质干细胞移植治疗小型猪急性心肌梗死的转归

“…【目的】 探讨超顺磁性氧化铁(SPIO)活体动态示踪小型猪骨髓间充质干细胞(MSC)移植治疗心肌梗死的可行性。【方法】 采用密度梯度离心法获取小型猪自体骨髓MSC,用携带绿色荧光蛋白(GFP)基因的慢病毒载体转染MSC,并用SPIO体外标记MSC-GFP。…”
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Reversal of Experimental Liver Damage after Transplantation of Stem-Derived Cells Detected by FTIR Spectroscopy by Danna Ye, Philip Heraud, Rangsun Parnpai, Tong Li

“…The aim of this study was to compare the efficiency of transplanted rBM-MSCs and rBM-MSC-derived differentiated stem cells (rBM-MSC-DSCs) for suppression of dimethylnitrosamine-injured liver damage in rat model. …”
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Mesenchymal Stem Cell–Sourced Exosomes as Potentially Novel Remedies for Severe Dry Eye Disease by Carl Randall Harrell, Valentin Djonov, Ana Volarevic, Aleksandar Arsenijevic, Vladislav Volarevic

“…Accordingly, MSC-Exos may modulate the progression of inflammatory eye diseases, including DED. …”
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骨髓间充质干细胞两种示踪方法的优缺点比较

“…【目的】 探讨荧光染料CM-DiI直接标记和慢病毒感染法EGFP标记人骨髓间充质干细胞(hMSC)用于体内示踪的优缺点65377;【方法】 利用FUGW质粒构建携带EGFP基因的慢病毒载体,并感染P3代hMSC,荧光倒置显微镜下观察细胞的感染效果及传代前后细胞EGFP表达情况的变化;CM-DiI直接标记P3代hMSC,观察CM-DiI 标记对细胞生物学特性的影响及传代前后细胞标记效果的变化65377;分别将CM-DiI和EGFP标记的P3代hMSC移植入新生大鼠左侧脑室,2周后取大鼠脑组织行冰冻切片,比较两种标记方法用于体内示踪的效果65377;【结果】 慢病毒感染hMSC 48 h后,约40%细胞表达绿色荧光,体外培养1个月,EGFP阳性细胞数增至50%左右,荧光强度基本保持不变,细胞生长不受病毒转染的影响65377;CM-DiI标记hMSC 12 h后,95%以上细胞呈现很强的红色荧光,1个月后,阳性细胞减少至50%左右,荧光强度也有一定程度降低,未见染料对细胞形态和生长产生明显影响65377;CM-DiI标记的hMSC移植后2周脑冰冻切片可以找到红色荧光的标记细胞, EGFP标记的hMSC移植后2周脑冰冻切片未找到绿色荧光细胞65377; 【结论】 CM-DiI和EGFP均可在体外高效标记hMSC,但CM-DiI 标记的hMSC体内示踪效果优于EGFP标记65377;…”
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