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Enhancing the Yield of <i>Pleurotus ostreatus</i> Through the Addition of Nucleotides and Nucleosides
Published 2025-07-01“…Furthermore, this research has disclosed that nucleotides and nucleosides exert a notable influence on the lignocellulolytic enzyme system. This investigation provides a scientific foundation for the development of novel yields—enhancing agents for <i>P. ostreatus</i> and offering new insights into cultivation techniques for the progress of <i>P. ostreatus</i> cultivation techniques in both academic and practical arenas.…”
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Gene Surfing: An efficient and versatile tool for targeted enzyme mining in metagenomics
Published 2025-12-01“…Validation results include: Cellulose-degrading enzymes (GH5 family): 1,311,316 potential lignocellulolytic enzyme sequences were identified, with 127 sequences functionally validated (84.25 % activity rate); Polyethylene-degrading enzymes: 705 candidate sequences were found, 38 of which were heterologously expressed, showing an 81.5 % activity rate (31/38); Endonucleases (HNH superfamily): 585 potential sequences were retrieved, with 4 out of 7 tested showing activity (57.1 % success rate).…”
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Response Mechanism of Extracellular Laccase Activity of Lyophyllum decastes to Cultivation Substrates and Subculture
Published 2025-08-01“…Different fermentation substrates were employed to investigate the variation patterns of lignocellulolytic enzymes in Lyophyllum decastes and the changes in laccase activity in subculture. …”
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