A capsular polysaccharide from a healthy human microbiota member activates a Lag-3-NK cell axis to restrain colon cancer and augment immunotherapy by Allison M. Weis, Kaylyn M. Bauer, William W. Tang, Emmanuel Stephen-Victor, Rickesha Bell, D. Garrett Brown, H. Atakan Ekiz, Van Tran, Kendra A. Klag, Eric A. Swanson, Logan Barrios, Morgan Harwood, Jennifer H. Hill, Kyla S. Ost, Biljana Gigic, Martin Schneider, Jennifer Ose, Sheetal Hardikar, Adetunji T. Toriola, David Shibata, Christopher I. Li, Jane C. Figueiredo, Doratha A. Byrd, Erin M. Siegel, Kathleen Arnolds, Catherine Lozupone, Cornelia M. Ulrich, Ryan M. O’Connell, W. Zac Stephens, June L. Round

“…CRC is recalcitrant to immunotherapies; however, addition of B. uniformis restores response to α-CTLA-4 treatment in an NK cell-dependent manner. We report that high Lag-3 expression is associated with greater survival in CRC patients and that B. uniformis-mediated protection is reliant on Lag-3 in innate cells. …”
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Expression of immune checkpoint markers in peripheral blood lymphocytes of women with fibroadenoma of the breast by O. V. Kurmyshkina, T. M. Kulikovskaya, T. O. Volkova

“…Sample uptake was performed immediately before surgery, and samples were further analyzed by multicolor flow cytometry using monoclonal antibodies CD3-VioBlue, CD4/CD8-FITC, PD1-PE, PD-L1-PerCP-Cy.5.5, and TIM3/LAG3-APC. Each sample was incubated with 4 antibody combinations: CD3/CD4/PD1/PD-L1/TIM3, CD3/CD4/PD1/PD-L1/LAG3, CD3/CD8/PD1/PD-L1/TIM3, and CD3/CD8/PD1/PD-L1/LAG3. …”
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Functional systemic CD4 immunity is required for clinical responses to PD‐L1/PD‐1 blockade therapy by Miren Zuazo, Hugo Arasanz, Gonzalo Fernández‐Hinojal, Maria Jesus García‐Granda, María Gato, Ana Bocanegra, Maite Martínez, Berta Hernández, Lucía Teijeira, Idoia Morilla, Maria Jose Lecumberri, Angela Fernández de Lascoiti, Ruth Vera, Grazyna Kochan, David Escors

“…In these patients, CD4 T cells possessed significant proliferative capacities, low co‐expression of PD‐1/LAG‐3 and were responsive to PD‐1 blockade ex vivo and in vivo. …”
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Expression profiling of T cell and B cell activation genes and immunoglobulin G subclasses in Egyptian breast cancer patients by Yara Elsherif, Mona Rady, Hesham Abdelaziz, Mohamed El-Azizi

“…Immune checkpoint analysis revealed significant upregulation of CD274 (P = 0.0045), CD47 (P = 0.0432), CD276 (P = 0.0310) and TLR9 (P = 0.0081), while LAG3 (P = 0.0347) were significantly downregulated. …”
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Clonorchis sinensis-driven hepatocarcinogenesis via E2F1-CD24 transcriptional axis: mechanistic and therapeutic implications by Wen-Min Lu, Jin Yan, Zhao-Ji Liu, Yong Wu, Qian-Ru Cui, Ji Feng, Yu Chen, Guang-Zhi Zhu, Tao Peng, Jing Zhou, Guo-Dong Lu

“…Additionally, the expression of immune checkpoint (CTLA-4, LAG-3) was assessed in the co-cultures of peripheral blood mononuclear cells (PBMCs)–HCC cells. …”
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Progress and challenges in melanoma immunotherapy by LIN Jing, CHEN Yaying, CHEN Yu

“…Meanwhile, emerging checkpoint inhibitors, including LAG-3, TIM-3, and TIGIT, have demonstrated promising new therapeutic effects. …”
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Investigating age-related dynamics and transcriptional signatures of CD8⁺HLA-DR⁺ regulatory T lymphocytes: perspectives in understanding immune system aging by K. S. Matveeva, S. A. Rybtsov, D. V. Shevyrev

“…It is known that the suppressive effects of these cells depend on intercellular interactions and are mediated by the expression of checkpoint inhibitor molecules such as CTLA-4, TIM-3, PD-1 and LAG-3. The CD8+HLA-DR+ regulatory T cells also share some properties with conventional CD4+ regulatory T lymphocytes. …”
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COVID-19 Outbreak Related to PM10, PM2.5, Air Temperature and Relative Humidity in Ahvaz, Iran by Yusef Omidi Khaniabadi, Pierre Sicard, Bahram Dehghan, Hassan Mousavi, Saeid Saeidimehr, Mohammad Heidari Farsani, Sadegh Moghimi Monfared, Heydar Maleki, Hojat Moghadam, Pouran Moulaei Birgani

“…The highest correlation with the number of COVID-19 cases was found at lag3 (r = 0.42) and at lag0 with a positive chest CT scan (r = 0.56). …”
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Differential quantities of immune checkpoint-expressing CD8 T cells in soft tissue sarcoma subtypes by Cornelis Verhoef, Reno Debets, Yarne Klaver, Stefan Sleijfer, Dirk Grünhagen, Rebecca Wijers, Maud Rijnders, Astrid Oostvogels, Marcel Smid, Cor Lamers

“…These TILs coexpress the immune checkpoints PD1, TIM3, and LAG3 in myxofibrosarcoma and pleomorphic sarcoma, yet TILs coexpressing these checkpoints were near negligible in GIST. …”
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Integrated molecular and clinical characterization of pulmonary large cell neuroendocrine carcinoma by Amin H. Nassar, Chul Kim, Tolulope Adeyelu, Elias Bou Farhat, Hassan Abushukair, Mehrdad Rakaee, Kelsey Matteson, Shun-Fat Lau, Yamato Takabe, Antonio Ocejo, Fatemeh Ardeshir-Larijani, Ticiana Leal, Suresh Ramalingam, Sumaiya Alam, Jhanelle E. Gray, James Hicks, David Kaldas, Javier Baena, Maria Zurera Berjaga, Frank Aboubakar Nana, Christian Grohe, Heike Leuders, Fabrizio Citarella, Alessio Cortellini, Emanuele Claudio Mingo, Danny Pancirer, Millie Das, Timothy John Ellis-Caleo, Justin M. Cheung, Jessica J. Lin, Alexander S. Watson, D. Ross Camidge, Arthi Sridhar, Kaushal Parikh, Fionnuala Crowley, Thomas U. Marron, Vanya Aggarwal, Murtaza Ahmed, Kamya Sankar, Hassan Kawtharany, Jun Zhang, Dwight H. Owen, Mingjia Li, Misako Nagasaka, David J. Pinato, Nichola Awosika, Khaled Alhamad, Sonam Puri, Unaiza Zaman, Divya M. Gupta, Chelsea Lau, Hina Khan, Justin Liauw, Ana I. Velazquez, Tyiesha Brown, Laura Moliner, Miguel Mosteiro, Pedro Rocha, Mark Evans, Ari Vanderwalde, Andrew Elliott, Jorge Nieva, Gilberto Lopes, Patrick C. Ma, Hossein Borghaei, Matthew Lee, Lauren Young, Raid Aljumaily, Haris Mirza, David J. Kwiatkowski, Roy S. Herbst, Richard A. Flavell, Abdul Rafeh Naqash, Anne C. Chiang

“…Here we show, elevated FGL-1 (a LAG-3 ligand) and SPINK1 expression in NSCLC-like LCNECs, and higher levels of DLL3 in SCLC-like LCNECs. …”
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Genomic and clinical characterization of pediatric lymphoepithelioma-like carcinoma by Yi Que, Xiuxia Lu, Suying Lu, Feifei Sun, Jia Zhu, Yu Zhang, Juan Wang, Junting Huang, Wei Liu, Fenghua Wang, Liping Li, Li Zhang, Min Gao, Zijun Zhen, Yizhuo Zhang

“…Significant upregulation of immune checkpoint molecules was detected including LAG-3, PD-L1, and galectin-9 in LELC group by proteomic analysis (P < 0.05). …”
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Study on Groundwater-Recharge Test for Deep Excavation of Subway Station in Water-Rich Sandy Strata by LI Weihua; LIU Tianren; HONG Xiaoxing; JING Xucheng; TAN Yong

“…The test results show that: 1) Although the aquitard is missing, the hydraulic connection between the upper soil layers((3)2-silt and(3)4-fine silt) is more closely related, and the connection with the lower soil layer((6)-silt) is weak; 2) The surface subsidence caused by dewatering is lagging; 3) After the pumping is stopped, the water level recovers fast. …”
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