Lysosomes finely control macrophage inflammatory function via regulating the release of lysosomal Fe2+ through TRPML1 channel by Yanhong Xing, Meng-meng Wang, Feifei Zhang, Tianli Xin, Xinyan Wang, Rong Chen, Zhongheng Sui, Yawei Dong, Dongxue Xu, Xingyu Qian, Qixia Lu, Qingqing Li, Weijie Cai, Meiqin Hu, Yuqing Wang, Jun-li Cao, Derong Cui, Jiansong Qi, Wuyang Wang

“…Here, we describe an unrecognized role for the lysosome, showing that it finely controls macrophage inflammatory function by manipulating the lysosomal Fe2+—prolyl hydroxylase domain enzymes (PHDs)—NF-κB—interleukin 1 beta (IL1B) transcription pathway that directly links lysosomes with inflammatory responses. …”
Get full text

HIF-1α and VEGF Immunophenotypes as Potential Biomarkers in the Prognosis and Evaluation of Treatment Efficacy of Atherosclerosis: A Systematic Review of the Literature by Dimitra P. Vageli, Panagiotis G. Doukas, Dimitrios Georgiou, Michailangelos P. Prokopiou, Nefeli E. Ladaki, Androniki Papadopoulou, Sotirios G. Doukas, Konstantina Zacharouli, Konstantinos P. Makaritsis, Maria Ioannou

“…Increased HIF-1α in vascular smooth muscle cells, macrophages, or endothelial cells, under hypoxia, chronic loss of nitric oxide (NO), or reduced micro ribonucleic acid (miRNA)-17 and miR-20, is associated with the upregulation of pro-inflammatory molecules, such as interleukin-1 beta (IL-1β) or tumor necrosis factor-alpha (TNF-α), increased migration inhibitory factor of macrophages, glycolytic flux, lipid accumulation, necroptosis via miR-383, and adverse effects in atherosclerosis and plaque vulnerability. …”
Get full text

Protective effects of 18β-glycyrrhetinic acid on Pasteurella multocida–induced vascular inflammatory injury in mice by Qirong Lu, Qirong Lu, Luyao Wang, Luyao Wang, Xueping Jiang, Xueping Jiang, Wantong Han, Wantong Han, Pu Guo, Pu Guo, Yu Liu, Yu Liu, Shulin Fu, Shulin Fu, Jianglin Xiong, Jianglin Xiong, Zhongyuan Wu, Zhongyuan Wu, Yinsheng Qiu, Yinsheng Qiu

“…These protective effects may be related to the reduced expression of poly (ADP-ribose) polymerase-1, high mobility group box 1, interleukin-1β, and interleukin-18 in vascular tissue by GA. …”
Get full text

Intervention effect and mechanism of Dracocephalum moldavica L. extract on bleomycin-induced pulmonary fibrosis in rats by Xiaoyu SUN, Li CHEN, Ruifang GAO, Hui YU, Min JIN

“…Enzyme-linked immunosorbent assay (ELISA) was used to detect tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) in rats. ResultsA total of 378 key chemical components of the Dracocephalum moldavica L. extract and 1611 lung fibrosis-related targets were identified. …”
Get full text

Protective Mechanism of Alisol A 24-Acetate on Brain Microvascular Endothelial Cells in Mice with Cerebral Ischemia-Reperfusion Injury by TRPM2/Akt/GSK3β Pathway by LI Huihong, DENG Yunfei, WEI Wei, ZHAN Zengtu, LIN Libin, XUE Xiehua

“…Modified neurological severity score (mNSS) was used to evaluate neurological deficit; novel object recognition test (NORT) and Morris water maze (MWM) were used to evaluate the cognitive function; immunofluorescence was used to detect the expression of CD31 in cortex; Western blot was used to detect zonula occludens-1 (ZO-1), Occludin, Claudin-5, phosphorylated nuclear factor kappa-B (p-NF-κB), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), transient receptor potential melastatin-2 (TRPM2), phosphorylated protein kinase B (p-Akt), phosphorylated glycogen synthetase kinase-3β (p-GSK3β) protein expression levels.Results(1) Neurological function: compared with the sham surgery group, mNSS in the model group significantly increased at the 1st, 3rd, 5th and 7th day (<italic>P</italic>&lt;0.05); compared with the model group, mNSS in the 24A group significantly decreased at the 3rd and 7th day (<italic>P</italic>&lt;0.05). (2) Cognitive function: compared with the sham surgery group, the NORT recognition index at 1st and 24th hour in the model group was significantly lower (<italic>P</italic>&lt;0.05); compared with the model group, the 1-hour and 24-hour NORT recognition index in the 24A group significantly increased (<italic>P</italic>&lt;0.05). …”
Get full text

Safety and efficacy of low-dose esketamine weakly opioidized anesthesia in elderly patients with lumbar spinal stenosis undergoing surgery: a prospective, double-blind randomized c... by Ni-Na Hou, Meng-Yun Zhang, Yu-Wei Zhang, Hong-Jing Wu, Hong Luo, Heng Yang

“…Pain (VAS rest and movement scores) on preoperative day 1 (POD-1), postoperative day 1 (POD1), postoperative day 3 (POD3), and postoperative day 7 (POD7), and serum levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and interleukin-10 (IL-10) on POD-1, POD1, POD3, and POD7 were the secondary outcomes. …”
Get full text

Potential Antidepressant Effects of Omeprazole Introduced Through Network Analysis and Systems Biology Should Be Interpreted with Caution in the Clinical Environment by Seyed-Ali Mostafavi

“…The authors have introduced the Omeprazole’s association with Tumor Necrosis Factor-α (TNF), and Interleukin-1β (IL-1 β) genes as the underlying mechanism for this proposal. …”
Get full text

Correlations between primary tumour location, biomarkers of inflammation and lung injury, and postoperative pulmonary complications in patients underwent laparoscopic colorectomy:... by Hui-xian Li, Li Che, Yuan Li, Tai-hang Wang, Fang-di Min, Lei Xu, Meng Wang, Zhao-xu Zheng, Shi-ning Qu, Fei Wang, Wei Tang, Shi-jing Wei, Yu-lin Sun, Hui Zheng, Tao Yan

“…Additionally, assessments have been performed for plasma biomarkers of immune response dynamics and lung injury (plasma soluble advanced glycation end-product receptor [sRAGE], angiopoietin-2 [ANG-2], interleukin-1β/6 [IL-1β/IL-6]) and other parameters.ResultsAlthough the increase in postoperative lung epithelial damage, as indicated by the plasma sRAGE levels, was significant in the RSC group (DAC vs. …”
Get full text

Effect of Berberine on the Inflammatory Response of ANA-1 Cells Induced by Angiotensin Ⅱ Based on TLR4 Signaling Pathway by GAO Dexing, JIA Peizhi, PENG Jun, LIN Wei

“…Cell proliferation and toxicity test (CCK-8) was used to detect the effect of different concentrations of BBR on cell viability; the flow cytometry was used to detect the mean fluorescence intensity (MFI) of TLR4 on cell membrane ; ELISA method was used to detect the content of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α); the real-time quantitative PCR was used to detect the mRNA level of IL-1β, IL-6, TNF-α; Western blot was used to detect the phosphorylation of p-ERK/ERK, p-p38/p38, p-JNK/JNK, p-p65/p65 and p-IRF3/IRF3 and the expression of TLR4, MyD88 and AP-1; the biochemical assay was used to detect the content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD).Results① CCK-8 assay results: compared with the control group, there was no significant difference in cell activity of the low, medium and high- dose BBR group, the difference was not statistically significant (<italic>P</italic>&gt;0.05). ② Flow cytometry results: compared with the fluorescent antibody group, MFI of the model group was significantly decreased; compared with the model group, MFI of the model group + the high dose BBR group was significantly increased, the difference was statistically significant (<italic>P</italic>&lt;0.05). ③ ELISA results: compared with the control group, the content of IL-1β, IL-6, TNF- α of the model group was significantly increased; compared with the model group, the content of IL-1β, IL-6, TNF- α of the model group + the low, medium and high dose BBR group was significantly decreased, the difference was statistically significant (<italic>P</italic>&lt;0.05). ④ Real-time quantitative PCR results: compared with the control group, the mRNA level of IL-1β, IL-6, TNF-α of the model group was significantly increased; compared with the model group, the mRNA level of IL-1β, IL-6, TNF- α of the model group + the low, medium and high-dose BBR group was significantly decreased, the difference was statistically significant (<italic>P</italic>&lt;0.05). ⑤ Western blot results: compared with the control group, the phosphorylation expression of p65 and IRF-3 protein of the model group after intervention for 45 minutes was significantly increased, and the phosphorylation expression of p65, IRF-3 protein in the model group + the low, medium and high-dose BBR group was significantly decreased. …”
Get full text

Clinical features and diagnostic criteria of diseases of esophagogastroduodenal zone in patients with gallstone disease after cholecystectomy by I. V. Kozlova, Ye. V. Graushkina

“…Parameters of concentration of interleukins 1, 8, 10, elastase, glycosaminoglycans, proteinbound hydroxy-proline in blood serum can be used as additional criteria of noninvasive diagnostics of gastroduodenal diseases in postcholecystectomy patients.…”
Get full text

Microglia and Spinal Cord Synaptic Plasticity in Persistent Pain by Sarah Taves, Temugin Berta, Gang Chen, Ru-Rong Ji

“…Increasing evidence also suggests an important role of spinal cord microglia in the genesis of persistent pain, by releasing the proinflammatory cytokines tumor necrosis factor-alpha (TNFα), Interleukine-1beta (IL-1β), and brain derived neurotrophic factor (BDNF). …”
Get full text

Role of the Kaiso gene in the development of inflammation in Mucin-2 defcient mice by E. A. Litvinova, K. M. Achasova, M. A. Borisova, S. V. Zhenilo, E. B. Prokhortchouk, E. N. Kozhevnikova

“…However, in the experimental model of IBD, Kaiso protein did not play a signifcant role in the regulation of pro-inflammatory cytokines of tumor necrosis factor and interleukins 1 and 17.…”
Get full text

Application of Computer Simulation in Exploring the Effects of <italic>Achyranthis Bidentatae Radix</italic> on Simultaneously Treating Wei Syndrome and Bi Syndrome of Osteoarthrit... by Chunsong ZHENG, Hongzhi YE, Jinxia YE, Changlong FU

“…Based on the platforms of quantitative structure-activity relationship and principal component analysis, the distributions of the above datasets were analyzed in the chemical space.②Matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-3 (MMP-3), and transforming growth factor beta-1 (TGF-β1) were chosen as the targets of treating Wei Syndrome of OA, while interleukine-1 beta (IL-1β), interleukine-6 (IL-6), tumor necrosis factor-alpha (TNF-α) were chosen as the targets of treating Bi syndrome of OA. …”
Get full text