Functional genomics implicates ebony in the black pupae phenotype of tephritid fruit flies by Daniel F. Paulo, Thu N. M. Nguyen, Chris M. Ward, Renee L. Corpuz, Angela N. Kauwe, Pedro Rendon, Rocio E. Y. Ruano, Amanda A. S. Cardoso, Georgia Gouvi, Elisabeth Fung, Peter Crisp, Anzu Okada, Amanda Choo, Christian Stauffer, Kostas Bourtzis, Sheina B. Sim, Simon W. Baxter, Scott M. Geib

“…In tephritids, understanding the molecular basis of mutant traits is also crucial for applied entomology, enabling the creation of genetic sexing strains through genome editing, thus facilitating sex-sorting before sterile insect releases. …”
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Supplemental Intervention for Alcohol Use Disorder Treatment Patients With a Co-Occurring Anxiety Disorder: Technical Development and Functional Testing of an Autonomous Digital Pr... by Linda Marie Rinehart, Justin Anker, Amanda Unruh, Nikki Degeneffe, Paul Thuras, Amie Norden, Lilly Hartnett, Matt Kushner

“…This process included: (1) identifying appropriate collaborators and vendors; (2) consultation with an e-learning expert to develop a storyboard and accompanying graphics and narrative; (3) video production and editing; and (4) interactive programming. The second phase of the project was functional testing of the newly developed digital intervention conducted in 52 residential AUD treatment patients with a comorbid anxiety disorder. …”
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Value Propositions for Digital Shared Medication Plans to Boost Patient–Health Care Professional Partnerships: Co-Design Study by Benjamin Bugnon, Francesca Bosisio, Alain Kaufmann, Pascal Bonnabry, Antoine Geissbuhler, Christian von Plessen

“…Participants discussed types of patient involvement in editing shared information and emphasized the importance of tailoring SMPs to individual abilities and preferences to foster health equity. …”
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The Undocumented Experience by Izah Tahir

“…In fact, I was the one editing their résumés. Like many students in this situation, I worked until I burnt myself out, and then I worked some more. …”
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First note of QTL mapping of low vigor traits using the updated F2 ‘Koroneiki’ linkage map of olive by Irene Granata, Aparna S. Balan, Aparna S. Balan, Claudio Di Vaio, Antonino Ioppolo, Tiziano Caruso, Silvia Fretto, Jubina Benny, Antonio Giovino, Daniel James Sargent, Francesco Paolo Marra, Annalisa Marchese

“…By integrating reference genomes with our genetic analysis, we offer a conceptual advancement that could substantially accelerate breeding timelines compared to traditional approaches. Although genome editing is still a future possibility due to the complexity of olive genetics and the species’ recalcitrance to transformation, our study lays a foundational understanding to guide future breeding programs. …”
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Phage-mediated virulence loss and antimicrobial susceptibility in carbapenem-resistant Klebsiella pneumoniae by Yanshuang Yu, Mengzhu Wang, Liuying Ju, Minchun Li, Mengshi Zhao, Hui Deng, Christopher Rensing, Qiu E. Yang, Shungui Zhou

“…Herein, we conducted evolutionary experiments, genomic analysis, and CRISPR-mediated gene editing, to illustrate the evolutionary trajectory occurring between phages and their hosts. …”
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Jovens e os Stories do Instagram: estudo netnográfico durante a pandemia da Covid-19 by Alan César Belo Angeluci, Brasilina Passarelli, Carolina Gois Falandes, Milena Possar Garcia

“…In Dix, everyday posts, humour, and sensitive topics prevailed, with less image editing, anticipating the photo dump phenomenon. …”
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Advances and Challenges in Antiviral Development for Respiratory Viruses by Luis Adrián De Jesús-González, Moisés León-Juárez, Flor Itzel Lira-Hernández, Bruno Rivas-Santiago, Manuel Adrián Velázquez-Cervantes, Iridiana Monserrat Méndez-Delgado, Daniela Itzel Macías-Guerrero, Jonathan Hernández-Castillo, Ximena Hernández-Rodríguez, Daniela Nahomi Calderón-Sandate, Willy Salvador Mata-Martínez, José Manuel Reyes-Ruíz, Juan Fidel Osuna-Ramos, Ana Cristina García-Herrera

“…The analysis also covers emerging technologies such as nanomedicine and CRISPR gene editing, which promise to improve the stability and efficacy of treatments. …”
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Using Quantitative Trait Locus Mapping and Genomic Resources to Improve Breeding Precision in Peaches: Current Insights and Future Prospects by Umar Hayat, Cao Ke, Lirong Wang, Gengrui Zhu, Weichao Fang, Xinwei Wang, Changwen Chen, Yong Li, Jinlong Wu

“…This study examines the complex genetic structure that underlies the morphology of peach fruits, paying special attention to the interaction between genome editing, genomic selection, and marker-assisted selection. …”
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The global trends and distribution in tumor-infiltrating lymphocytes over the past 49 years: bibliometric and visualized analysis by Beibei Wu, Beibei Wu, Beibei Wu, Ding Luo, Ding Luo, Xuejie Wang, Xuejie Wang, Chen Qiao, Chen Qiao, Rui Li, Jian Liu, Jian Liu

“…Combination therapies; tumor neoantigens; gene editing; dominant population selection of TILs therapy; TILs in Tumor microenvironment; emerging predictive biomarkers; TILs in predicting the efficacy of neoadjuvant chemotherapy and immunotherapy; the relationship between TILs and PD-L1; TIL-based patient stratification; tertiary lymphoid structures; and TIL evaluation through digital pathology and artificial intelligence are identified as key areas of interest.ConclusionsThis analysis highlights the increasing academic focus on tumor-infiltrating lymphocyte research and identifies key recent themes in the field such as prognostic value of TILs, personalized treatments, and combination therapies.…”
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Comprehensive analysis of mRNA and miRNA differential expression profiles in the hypothalamus-pituitary-gonadal axis in laying and broodiness period of Wanxi white geese by Xinwei Tong, Xiaojin Li, Yuhua Wang, Fei Xie, Ruidong Li, Man Ren, Qianqian Hu, Shenghe Li

“…Several miRNAs and mRNAs associated with the reproductive performance of WWG were identified, providing a crucial reference for the subsequent use of gene editing technologies to breed new varieties and advance the development of WWG breeding industry.…”
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Identification of highly expressed genes and efficient core promoters specific to buffalo skeletal muscles by J. Huang, J. Huang, D. Guo, R. Zhu, H. Wang, C. Yang, D. Shi, J. Shang

“…This is of great significance for the targeted improvement of livestock meat quality by combining gene editing and traditional breeding techniques. To identify efficient promoters specific to the skeletal muscles of buffalo, in the present study, a total of 14 genes, <i>CACNG1</i>,<i> LRRC30</i>,<i> CACNG6</i>,<i> MYOG</i>,<i> VGLL2</i>,<i> MYOD1</i>,<i> KCNA7</i>,<i> DUPD1</i>,<i> PRR32</i>,<i> LBX1</i>,<i> IGFN1</i>,<i> ACTN3</i>,<i> PITX3</i>, and<i> MURC</i>, were firstly screened as skeletal-muscle-specific expressed genes based on high-throughput sequencing data. …”
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Silk Protein Gene Engineering and Its Applications: Recent Advances in Biomedicine Driven by Molecular Biotechnology by Ji X, Li Y, Wang J, Wang G, Ma B, Shi J, Cui C, Wang R

“…This review outlines the roles of major model organisms, such as silkworms and spiders, in silk protein production, and provides a detailed introduction to the applications of gene editing technologies (eg, CRISPR-Cas9), transgenic expression technologies, and synthetic biology techniques in silk protein genetic engineering. …”
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Self-Management Strategies for Low Back Pain Among Horticulture Workers: Protocol for a Type II Hybrid Effectiveness-Implementation Study by Kim Dunleavy, Heidi Liss Radunovich, Jason M Beneciuk, Boyi Hu, Yang Yang, Janeen McCormick Blythe, Kelly K Gurka

“…The design, creation, and editing of English and Spanish videos was completed in June 2024 after comprehensive formative evaluation. …”
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Case report: The case of T-cell acute lymphoblastic leukemia treated with chemotherapy followed by anti-CD7 CAR-T cells using retroviral vector by Huanhuan Zhou, Wenxiang Zhu, Qihong Ma, Ning Liu, Mengdi Jin, Yaru Feng, Lijun Zhao, Rui Sun, Rongyou Li, Huaxiu Li, Yuanyuan Shi, Jianxun Wang, Liqiong Liu, Zhi Guo, Zhi Guo, Zhi Guo

“…This case report of clinical treatment of T-ALL with anti-CD7 CAR-T cells prepared using a retroviral vector without gene editing and combined with chemotherapy, which demonstrated that the RV-based anti-CD7 CAR-T cells had good therapeutic effect and high safety in triple-refractory T-ALL patients.…”
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Genetic disruption of Blimp-1 drastically augments the antitumor efficacy of BCMA-targeting CAR T cells by Anthony M. Battram, Joan Mañé-Pujol, David F. Moreno, Aina Oliver-Caldés, Judit Carpio, Oriol Cardus, Luis Gerardo Rodríguez-Lobato, Álvaro Urbano-Ispizua, Carlos Fernández de Larrea

“…To improve long-term efficacy, we used CRISPR/CRISPR-associated protein 9-mediated gene editing to ablate the expression of the transcription factor Blimp-1. …”
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Galectin-3 secreted by triple-negative breast cancer cells regulates T cell function by Annat Raiter, Yael Barhum, Julia Lipovetsky, Chen Menachem, Sharona Elgavish, Shmuel Ruppo, Yehudit Birger, Shai Izraeli, Orna Steinberg-Shemer, Rinat Yerushalmi

“…Here, we further investigated the interaction between secreted Gal-3 and T cells in TNBC.Using CRISPR/Cas9 gene editing of the TNBC MDA-MB-231 cell-line, we obtained Gal-3 negative(neg) clones. …”
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LLT1 overexpression renders allogeneic-NK resistance and facilitates the generation of enhanced universal CAR-T cells by Shuxian Zhu, Shiyu Zuo, Chuo Li, Xingjie You, Erlie Jiang, Xiaoming Feng, Yuechen Luo

“…We performed cytotoxicity, proliferation, and cytokine assays to evaluate the functionality of universal chimeric antigen receptor-T cell (UCAR-T) cells and conducted in vitro and in vivo assays, including allogeneic responses and RNA sequencing, to assess their resistance to allogeneic T and NK cells, anti-leukemia efficacy, and persistence in treating hematologic malignancies. Results Genetic editing of CD38 universal CAR-T cells, including CD38, T cell receptor alpha constant (TRAC), beta-2-microglobulin (B2M), and class II major histocompatibility complex transactivator (CIITA) knockdowns, was successfully achieved. …”
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Qualitative and quantitative characteristics of black patch trait in Oujiang color common carp by Ke Jing, Huifan Chen, Nusrat Hasan Kanika, Penghui Zhang, Roland Nathan Mandal, Chunxiao Zeng, Zhiyi Zhang, Jun Wang, Chenghui Wang

“…These results demonstrated that the black patches exhibits quantitative traits in nature. Gene editing on Ednrb by using the CRISPR/Cas9 system showed that the mutants displayed a significantly reduced number of black patches in comparison to the wild-type RB, accompanied by noticeably smaller melanocytes. …”
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