Comprehensive phenotypic analysis of multiple gene deletions of α-glucan synthase and Crh-transglycosylase gene families in Aspergillus niger highlighting the versatility of the fu... by Katharina J. Ost, Mark Arentshorst, Bruno M. Moerschbacher, Mareike E. Dirks-Hofmeister, Arthur F.J. Ram

“…To determine whether the ags and crh gene families have redundant functions, both gene families were deleted using iterative CRISPR/Cas9 mediated genome editing. The 12-fold deletion mutant was viable and did not exhibit growth defects under non-stressing growth conditions. …”
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PMA1-containing extracellular vesicles of Candida albicans triggers immune responses and colitis progression by Zhen Xu, Shuping Qiao, Zelin Wang, Chen Peng, Yayi Hou, Baorui Liu, Guochun Cao, Tingting Wang

“…A Crispr-Cas9-based fungal strain editing system was then used to knock out PMA1 expression in C. albicans. …”
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Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton by Brendan W. Mormile, Yan Yan, Taran Bauer, Li Wang, Rachel C. Rivero, Sara C. D. Carpenter, Catherine Danmaigona Clement, Kevin L. Cox, Lin Zhang, Xiyu Ma, Terry A. Wheeler, Jane K. Dever, Ping He, Adam J. Bogdanove, Libo Shan

“…Transcriptome profiling coupled with TALE-binding element prediction identify a pectin lyase gene as an additional Tal7b target, quantitatively contributing to Xcm virulence alongside GhSWEET14a/b. CRISPR-Cas9 gene editing supports the function of GhSWEETs in cotton bacterial blight and the promise of disrupting the TALE-binding site in S genes for disease management. …”
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Genomic Characterization and Functional Validation of Six <i>cis</i>-Regulatory Sequences in Medicinal Plant <i>Andrographis paniculata</i> by Xingbin Lv, Hua Yang, Yufang Hu, Qi Liang, Shuyun Tian, Lang Yang, Mingkun Huang, Ling Zhang, Yanqin Xu

“…With the rapid development of plant biotechnologies, such as transgenesis and gene editing tools, plant-derived CRSs have gradually replaced traditional CRSs, like the <i>35S</i> promoter, to avoid some negative effects caused by exogenous DNA sequences. …”
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Virtual choir as a kind of digital musical art by Xinbin Fu

“…A virtual choir does not require many purely choral skills to achieve a result (ensemble, tuning, responding to the conductor’s gesture and singing by colleagues in the part), but requires significant technical knowledge and skills (video and audio recording, editing, correction, etc.). Thus, virtual choir as a phenomenon of modern art is not so much a type of choral performance as its simulation, carried out using digital methods, and should be studied in accordance with its belonging to one of the types of digital art. …”
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An Online Interactive Experiment Management System for CMA Numerical Weather Prediction Models by Wang Bin, Hu Jiangkai, Cui Yingjie, Zhou Bin, Sun Jing, Zhao Chunyan, Lou Panxing

“…The system offers functions for template editing, process arrangement, and instance construction, facilitating the flexible and rapid design of model experiments. …”
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Application of photogrammetry and laser scanner on the Bronze Age structures of the Castillejos de Luna cist tomb necropolis (Pizarra, Spain) by Alejandro Muñoz-Muñoz, Diego Fernández-Sánchez, Eduardo Vijande-Vila, Serafín Becerra-Martín, Juan Jesús Cantillo-Duarte, Salvador Domínguez-Bella, Virgilio Martínez Enamorado, Francisca Rengel Castro, Pedro Cantalejo Duarte, María del Mar Espejo-Herrerías, José Suárez-Padilla, Juan Antonio Martín-Ruiz, José Ramos-Muñoz

“…Using tools to document tombs in 3D offers us great advances in data acquisition and editing, with great precision and realism, thanks to the 3D models generated through techniques such as photogrammetry or laser scanning. …”
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Advances in plant male sterility for hybrid seed production: an overview of conditional nuclear male sterile lines and biotechnology-based male sterile systems by Naresh Vasupalli, Naresh Vasupalli, Kanakachari Mogilicherla, Kanakachari Mogilicherla, Vahab Shaik, Vahab Shaik, K. R. S. Sambasiva Rao, Shripad R. Bhat, Xinchun Lin, Xinchun Lin

“…Advances in crop genomics and discovery of a large number of nuclear genes governing anther/pollen development, which are shared across species, are helping design diverse types of male sterile lines suitable for different crop species and situations. In particular, gene editing offers quick and easy route to develop novel male sterility systems for hybrid seed production. …”
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Altered O-Glycans in stimulated whole saliva from patients with primary Sjögren’s syndrome and non-pSS sicca by Sarah Kamounah, Kristina A. Thomsson, Christiane Elisabeth Sørensen, Eric Paul Bennett, Niclas G. Karlsson, Anne Marie Lynge Pedersen

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Achievements and prospects of applying high-throughput sequencing techniques to potato genetics and breeding by I. V. Bykova, N. A. Shmakov, D. A. Afonnikov, A. V. Kochetov, E. K. Khlestkina

“…Large-scale studies of the potato genome and genes exploiting next-generation sequence (NGS) approaches are required for broad application of MAS, genomic selection as well as genomic editing (the newest approach for creating potato with desired properties). …”
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A guide for developing comprehensive systems biology maps of disease mechanisms: planning, construction and maintenance by Alexander Mazein, Marcio Luis Acencio, Irina Balaur, Adrien Rougny, Danielle Welter, Anna Niarakis, Anna Niarakis, Diana Ramirez Ardila, Ugur Dogrusoz, Piotr Gawron, Venkata Satagopam, Venkata Satagopam, Wei Gu, Wei Gu, Andreas Kremer, Reinhard Schneider, Reinhard Schneider, Marek Ostaszewski, Marek Ostaszewski

“…In this review, we offer a step-by-step guide for developing a disease map within its mainstream pipeline that involves using the CellDesigner tool for creating and editing diagrams and the MINERVA Platform for online visualisation and exploration. …”
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Polyploidization-driven transcriptomic dynamics in Medicago sativa neotetraploids: mRNA, smRNA and allele-specific gene expression by D. F. Santoro, G. Marconi, S. Capomaccio, M. Bocchini, A. W. Anderson, A. Finotti, M. Confalonieri, E. Albertini, D. Rosellini

“…Allele-specific expression analysis indicated that parent-of-origin bias was not a significant outcome of WGD, but we found that parentally biased RNA editing may be a significant source of variation in neopolyploids.…”
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De novo assembly of the complete mitochondrial genomes of two Camellia-oil tree species reveals their multibranch conformation and evolutionary relationships by Zhun Xiao, Yiyang Gu, Junqin Zhou, Mengqi Lu, Jinfeng Wang, Kaizheng Lu, Yanling Zeng, Xiaofeng Tan

“…We conducted codon usage and RNA editing site analysis on these two mitogenomes, which showed highly consistent results. …”
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Advances in Molecular Genetics of Reproductive Isolation in Rice by Shunmei LI, Zihan ZHENG, Ruiying LIU, Hongze GUO, Lin ZHENG, Yongyao XIE

“…According to these findings, several strategies and methods such as developing wide compatibility lines, breeding intersubspecific introgression rice and establishing artificial hybrid compatible lines by genome editing have been proposed to overcome RI in rice. …”
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Chicken C4BPM facilitates replication of H5N1 and H9N2 Avian Influenza Viruses by inhibiting the production of type I interferons by Huizi Li, Shishi Wang, Ruihong Guo, Xijiao Chen, Yuanyuan Huang, Weixin Jia, Zhangyong Ning

“…To verify the role of C4BPM in replication of AIVs, C4BPM knockout (C4BPM-KO) and stable overexpressing (C4BPM-overexpressing) DF-1 cells were generated using lentivirus-mediated CRISPR/Cas9 gene editing technology and molecular cloning strategies. …”
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Characterization of a chemically induced osteoarthritis model in zebrafish by Gongyi Xiao, Jin Qin, Huiping Yang, Qizhi Song, Ruobin Zhang, Junlan Huang, Yuexi Mou, Wen Liu, Xianding Sun, Mao Nie

“…Moreover, zebrafish present distinct advantages, including robust regenerative abilities, genetic editing simplicity, and efficient drug screening. …”
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Arte verbal del siglo XXI: la escritura como práctica artística post-literaria en el mundo contemporáneo by Adolfo Rodríguez Posada

“…La importancia clave que alcanza la experimentación con el medio verbal, en la producción vanguardista de los escritores españoles desde la segunda mitad del siglo XX hasta la actualidad y gracias a los nuevos soportes vinculados a la invención de Internet, las redes sociales y los dispositivos electrónicos, convierte en centrales, dentro de la nueva teoría del arte verbal español, aspectos tales como la materialidad de la escritura y su dimensión gráfica y concreta, técnicas y mecanismos de postproducción de textos como el collage, la apropiación y la derivación (détournement), el montaje (editing), la remezcla (mixing) o el sampleado (sampling), así como la performatividad que desmaterializa el objeto poético y reduce la práctica literaria a una acción artística conceptualista. …”
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Advancing date palm cultivation in the Arabian Peninsula and beyond: Addressing stress tolerance, genetic diversity, and sustainable practices by Soumya Koippully Manikandan, Dharshini Jenifer. A, Nisarga K. Gowda, Vaishakh Nair, Rami Al-Ruzouq, Mohamed Barakat A. Gibril, Fouad Lamghari, John Klironomos, Maryam Al Hmoudi, Mohamed Sheteiwy, Ali El-Keblawy

“…Biotechnological advancements, such as genetic transformation and genome editing, are discussed for their potential to enhance crop resilience and productivity. …”
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Use of large language models as artificial intelligence tools in academic research and publishing among global clinical researchers by Tanisha Mishra, Edward Sutanto, Rini Rossanti, Nayana Pant, Anum Ashraf, Akshay Raut, Germaine Uwabareze, Ajayi Oluwatomiwa, Bushra Zeeshan

“…Majority (57.5%) of these participants practiced in an academic setting with a median of 7 (2,18) PubMed Indexed published articles. 198 respondents (87.6%) were aware of LLMs and those who were aware had higher number of publications (p < 0.001). 18.7% of the respondents who were aware (n = 37) had previously used LLMs in publications especially for grammatical errors and formatting (64.9%); however, most (40.5%) did not acknowledge its use in their papers. 50.8% of aware respondents (n = 95) predicted an overall positive future impact of LLMs while 32.6% were unsure of its scope. 52% of aware respondents (n = 102) believed that LLMs would have a major impact in areas such as grammatical errors and formatting (66.3%), revision and editing (57.2%), writing (57.2%) and literature review (54.2%). 58.1% of aware respondents were opined that journals should allow for use of AI in research and 78.3% believed that regulations should be put in place to avoid its abuse. …”
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MaveDB 2024: a curated community database with over seven million variant effects from multiplexed functional assays by Alan F. Rubin, Jeremy Stone, Aisha Haley Bianchi, Benjamin J. Capodanno, Estelle Y. Da, Mafalda Dias, Daniel Esposito, Jonathan Frazer, Yunfan Fu, Sally B. Grindstaff, Matthew R. Harrington, Iris Li, Abbye E. McEwen, Joseph K. Min, Nick Moore, Olivia G. Moscatelli, Jesslyn Ong, Polina V. Polunina, Joshua E. Rollins, Nathan J. Rollins, Ashley E. Snyder, Amy Tam, Matthew J. Wakefield, Shenyi Sunny Ye, Lea M. Starita, Vanessa L. Bryant, Debora S. Marks, Douglas M. Fowler

“…Here we describe the 2024 update to MaveDB ( https://www.mavedb.org/ ) with four key improvements to the MAVE community’s database of record: more available data including over 7 million variant effect measurements, an improved data model supporting assays such as saturation genome editing, new built-in exploration and visualization tools, and powerful APIs for data federation and streamlined submission and access. …”
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