Transitioning from wet lab to artificial intelligence: a systematic review of AI predictors in CRISPR by Ahtisham Fazeel Abbasi, Muhammad Nabeel Asim, Andreas Dengel

“…CRISPR-Cas9 based genome editing is a multi-step process such as designing a precise gRNA, selecting the appropriate Cas protein, and thoroughly evaluating both on-target and off-target activity of the Cas9-gRNA complex. …”
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Advances in Nucleic Acid Drugs and Gene Therapies based on Animal Models of Duchenne Muscular Dystrophy by LIU Siyu, LAI Yuezhao, GUO Wenting, CHEN Xuejin

“…This review briefly outlines the pathological mechanisms and genetic characteristics of DMD before delving into recent progress in therapeutic strategies, with a particular focus on nucleic acid drugs (including antisense oligonucleotides for exon skipping therapy and translation readthrough inducers) and gene therapy approaches (including gene replacement therapy and gene editing). The development and application of these therapies not only provide new treatment options for DMD patients, but also offer valuable insights for addressing other genetic disorders. …”
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The plant germplasm and herbarium (WIR) collections maintained at VIR as contributors to grape genetic diversity conservation, expansion and utilization by M. M. Agakhanov, L. V. Bagmet, N. G. Tikhonova, M. V. Erastenkova, E. N. Kislin, Yu. V. Ukhatova, E. K. Khlestkina

“…Achievements in the application of gene editing technologies to increase grape resistance to diseases are discussed. …”
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GENOME POLITICS: STRATEGY AND TACTICS by Jonas Rubikas

“…For better realization of information in some cases the genome changes the usual way of expression, that is, the genome uses some tactical approaches: at the DNA level - restructuring of the genome (mating type in yeasts, sex determination in some flies and worms, V(D)J recombination in immunoglobulin genes in human B lymphocytes); at the RNA level - gene splicing, alternative and self splicing, RNA editing; at the protein level - protein splicing. …”
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Characterization of Mice Ubiquitously Overexpressing Human 15-Lipoxygenase-1: Effect of Diabetes on Peripheral Neuropathy and Treatment with Menhaden Oil by Lawrence Coppey, Alexander Obrosov, Hanna Shevalye, Eric Davidson, William Paradee, Mark A. Yorek

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The study of organelle DNA variability in alloplasmic barley lines in the NGS era by M. G. Siniauskaya, A. M. Makarevich, I. M. Goloenko, V. S. Pankratov, A. D. Liaudanski, N. G. Danilenko, N. V. Lukhanina, A. M. Shimkevich, O. G. Davydenko

“…Some of the SNPs detected are very similar in codon position and in the type of amino acid substitution to the places where RNA editing can occur. Thus, these results outline new perspectives for the future study of nuclear-cytoplasmic interactions in alloplasmic lines.…”
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THE MORPHOGENIC POTENTIAL OF SIBERIAN POTATO CULTIVARS IN TISSUE CULTURES by S. M. Ibragimova, A. V. Romanova, G. Kh. Myzgina, A. V. Kochetov

“…Recent advances in plant  biotechnology have made it possible to produce resistant cultivars by plant genome editing. This approach allows crop modiications that would be diicult to obtain by conventional breeding techniques. …”
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Potato spindle tuber viroid by A. V. Kochetov, A. Y. Pronozin, N. V. Shatskaya, D. A. Afonnikov, O. S. Afanasenko

“…This association between the small segments of viroid genomic RNAs complementary to the untranslated regions of cellular mRNAs and disease symptoms provides a way for new resistant cultivars to be developed by genetic editing. To conclude, viroids provide a unique model to reveal the fundamental features of living systems, which appeared early in evolution and still remain undiscovered.…”
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Effects of the auxin-dependent degradation of the cohesin and condensin complexes on the repair of distant DNA double-strand breaks in mouse embryonic stem cells by A. V. Smirnov, A. S. Ryzhkova, A. M. Yunusova

“…TIDE analysis revealed that editing frequency was consistent (30–50 %) for most experiments with a minor decrease after auxin addition. …”
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A novel approach to analyzing the evolution of SARS-CoV-2 based on visualization and clustering of large genetic data compactly represented in operative memory by A. Yu. Palyanov, N. V. Palyanova

“…Finally, the evolution of distributions of the number of changes (editing distance between each genome and reference sequence) caused by substitutions, deletions and insertions was visualized as 3D surfaces, which clearly show the process of viral evolution over 4.5 years, with a time step = 1 week. …”
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Cas12e orthologs evolve variable structural elements to facilitate dsDNA cleavage by Danyuan Li, Shouyue Zhang, Shuo Lin, Wenjing Xing, Yun Yang, Fengxia Zhu, Dingding Su, Chunlai Chen, Jun-Jie Gogo Liu

“…Two known Cas12e nucleases, DpbCas12e and PlmCas12e, are both effective in genome editing. However, many differences exist in their in vitro dsDNA cleavage activities, reflecting the diversity in Cas12e’s enzymatic properties. …”
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G-cleave LC3B biosensor: monitoring autophagy and assessing resveratrol's synergistic impact on doxorubicin-induced apoptosis in breast cancer cells by Chiao-Chun Liao, Yuqing Long, Ming-Lin Tsai, Chun-Yu Lin, Kai-Wen Hsu, Chia-Hwa Lee

“…The specificity of G-cleave LC3B biosensor was confirmed via CRISPR gene editing of pivotal autophagy regulator ATG4B, yielding diminished luciferase activity in MDA-MB-231 breast cancer cells. …”
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In silico methods for immunogenicity risk assessment and human homology screening for therapeutic antibodies by Aimee E. Mattei, Andres H. Gutierrez, Soorya Seshadri, Jacob Tivin, Matt Ardito, Amy S. Rosenberg, William D. Martin, Anne S. De Groot

“…Should modifications to the biologic sequence be an option, a tool for moderating putative immunogenicity by editing T cell epitopes out of the sequence is available (OptiMatrix). …”
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Animal Models for Human-Pathogenic Coronavirus and Animal Coronavirus Research by Fenglian Xiao, Jincheng Hu, Minsheng Xu, Di Wang, Xiaoyan Shen, Hua Zhang, Jie Miao, Haodong Cai, Jihui Wang, Yaqing Liu, Shan Xiao, Longchao Zhu

“…These approaches include using viral delivery methods to induce the expression of viral receptors in mouse tissues and employing gene-editing techniques to create genetically modified mice. …”
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Structural characteristics, functions, and counteracting strategies of biofilms in Staphylococcus aureus by Yanze Xia, Zhenghui Hu, Qiyuan Jin, Qi Chen, Chenhao Zhao, Rui Qiang, Zonggang Xie, Liubing Li, Haifang Zhang

“…Furthermore, the review provides a comprehensive overview of recent therapeutic innovations, including enzymatic therapy, nanotechnology, gene editing, and phage therapy. Key scientific concepts of review: Emerging therapeutic strategies present novel approaches to combat S. aureus biofilm-associated infections through various mechanisms. …”
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Exploration of the Role of Cyclophilins in Established Hepatitis B and C Infections by Jennifer Molle, Sarah Duponchel, Jennifer Rieusset, Michel Ovize, Alexander V. Ivanov, Fabien Zoulim, Birke Bartosch

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The mechanism of discriminative aminoacylation by isoleucyl-tRNA synthetase based on wobble nucleotide recognition by Bingyi Chen, Fang Yi, Zhiteng Luo, Feihu Lu, Hongwei Liu, Siting Luo, Qiong Gu, Huihao Zhou

“…ScIleRS utilizes a eukaryotic/archaeal-specific arginine as the H-bond donor to recognize the common carbonyl group (H-bond acceptor) of various N34s of tRNAIle, which induces mutual structural adaptations between ScIleRS and tRNAIle to achieve a preferable editing state. C34 of unmodified tRNAIle(CAU) (behaves like tRNAMet) lacks a relevant H-bond acceptor, which disrupts key H-bonding interactions and structural adaptations and suspends the ScIleRS·tRNAIle(CAU) complex in an initial non-reactive state. …”
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Influence of Information Technology on the Performance of Financial Institutions in Ntungamo: A Case Study of Centenary Bank Ntungamo Branch. by Murungi, Doreen

“…The study applied primary data using questionnaires and secondary data collected from the respective institution’s financial statements and summaries at the Centenary Bank Ntungamo branch. Editing, coding, classifying, and tabulation are the processing steps that were used to process data for better and more efficient analysis. …”
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<i>shp-2</i> gene knockout upregulates CAR-driven cytotoxicity of YT NK cells by V. G. Subrakova, S. V. Kulemzin, T. N. Belovezhets, A. N. Chikaev, N. A. Chikaev, O. A. Koval, A. A. Gorchakov, A. V. Taranin

“…We hypothesized that stronger cytotoxicity of NK cells could be achieved via gene editing aimed at enhancing the activating signaling cascades and/or weakening the inhibitory ones, thereby shifting the balance of signals towards NK cell activation and target cell lysis. …”
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Long-term correction of hemophilia A via integration of a functionally enhanced FVIII gene into the AAVS1 locus by nickase in patient-derived iPSCs by Do-Hun Kim, Sang-Hwi Choi, Jin Jea Sung, Sieun Kim, Hanui Yi, Sanghyun Park, Chan Wook Park, Young Woo Oh, Jungil Lee, Dae-Sung Kim, Jong-Hoon Kim, Chul-Yong Park, Dong-Wook Kim

“…Abstract Hemophilia A (HA) is caused by mutations in coagulation factor VIII (FVIII). Genome editing in conjunction with patient-derived induced pluripotent stem cells (iPSCs) is a promising cell therapy strategy, as it replaces dysfunctional proteins resulting from genetic mutations with normal proteins. …”
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