Comparison of metabarcoding taxonomic markers to describe fungal communities in fermented foods by Rué, Olivier, Coton, Monika, Dugat-Bony, Eric, Howell, Kate, Irlinger, Françoise, Legras, Jean-Luc, Loux, Valentin, Michel, Elisa, Mounier, Jérôme, Neuvéglise, Cécile, Sicard, Delphine

“…When comparing the three rDNA markers, ITS markers performed better than D1/D2, as they are better represented in public databases and have better specificity to distinguish species. …”
Get full text

The mutational landscape of ARMC5 in Primary Bilateral Macronodular Adrenal Hyperplasia: an update by Lucas Bouys, Anna Vaczlavik, Isadora P. Cavalcante, Florian Violon, Anne Jouinot, Annabel Berthon, Patricia Vaduva, Stéphanie Espiard, Karine Perlemoine, Peter Kamenicky, Marie-Christine Vantyghem, Antoine Tabarin, Gérald Raverot, Cristina L. Ronchi, Ulrich Dischinger, Martin Reincke, Maria C. Fragoso, Constantine A. Stratakis, Albain Chansavang, Eric Pasmant, Bruno Ragazzon, Jérôme Bertherat, for the COMETE and ENSAT Networks

“…The pathogenicity of ARMC5 variants is established on the basis of their frequency in the general population, in silico predictions, familial segregation and tumor DNA sequencing. Conclusions This is the first extensive review of ARMC5 pathogenic variants. …”
Get full text

Shorter telomere length in COPD cases secondary to biomass-burning smoke exposure by Angélica Domínguez-de-Barros, Gloria Pérez-Rubio, Ingrid Fricke-Galindo, Alejandra Ramírez-Venegas, Malena Gajate-Arenas, Rafael Hernández-Zenteno, Salvador García-Carmona, Robinson Robles-Hernández, María E. Ramírez-Díaz, Filiberto Cruz-Vicente, María L. Martínez-Gómez, Jacob Lorenzo-Morales, Ramcés Falfán-Valencia, Elizabeth Córdoba-Lanús

“…Lung function parameters were measured by spirometry, and relative telomere length (rTL) from peripheral blood DNA was determined using multiplex qPCR. Results Results showed rTL to inversely correlate with age (R2=-0.207, p = 0.006) and with the hours-a-day of BS exposure (R2=-0.297, p < 0.001). …”
Get full text

Farm-to-fork changes in poultry microbiomes and resistomes in Maputo City, Mozambique by Natalie Olson, Frederica Lamar, Hermógenes Mucache, José Fafetine, Joaquim Saíde, Amélia Milisse, Denise R. A. Brito, Kelsey J. Jesser, Karen Levy, Matthew C. Freeman, Maya L. Nadimpalli

“…Here, we performed metagenomic sequencing of total DNA from banked ChEEP ChEEP samples to characterize fecal and carcass microbiomes and resistome diversity between chicken breeds and along the supply chain. …”
Get full text

Exploring the Role of C-C Motif Chemokine Ligand-2 Single Nucleotide Polymorphism in Pulmonary Tuberculosis: A Genetic Association Study from North India by Sanjay K. Biswas, Mayank Mittal, Ekata Sinha, Vandana Singh, Nidhi Arela, Bharat Bajaj, Pramod K. Tiwari, Vishwa M. Katoch, Keshar K. Mohanty

“…The genotyping was carried out in 373 participants with pulmonary TB (PTB) and 248 healthy controls (HCs) for CCL2-2518 A>G and -362 G>C polymorphisms by PCR-RFLP and by melting curve analysis using fluorescence-labeled hybridization fluorescent resonance energy transfer (FRET) probes, respectively, followed by DNA sequencing in a few representative samples. …”
Get full text

Molecular identification of chitinase and its function in growth of Odontobutis potamophila by Siyu Lu, Lin Du, Ting Zhang, Dan Wang, Jinjuan Wan, Guoxing Liu, Yuning Hu, Yu Xu, Zhiqiang Xu, Xuguang Li

“…In this study, a full-length cDNA of chitinase gene (namely, OpCht) was obtained from dark sleeper Odontobutis potamophila. …”
Get full text

Comparative analysis of chloroplast genomes reveals molecular evolution and phylogenetic relationships within the Papilionoideae of Fabaceae by Qian Qin, Yanjing Dong, Jialong Chen, Bo Wang, Yuxin Peng, XinPeng Zhang, Xiaoyun Wang, Jinxiang Zeng, Guoyue Zhong, Shouwen Zhang, Xiaolang Du

“…Comparative genomic variations at cpDNA sites were identified. Moreover, by using D. elegans as a reference, six genes (ycf4, clpP, ycf1, trnI-GAU, accD, rpl32) displayed high nucleotide polymorphism (Pi > 0.1), and the Ka/Ks ratio for all protein-coding genes was determined to be less than 1. …”
Get full text

High-fat diet-induced liver injury in Channa argus through lipid metabolism, anti-oxidative status, apoptosis, and inflammation by Jiaxin Tian, Ying Li, Jiwu Wan, Zhinan Yang, Guiqin Wang

“…Anti-oxidative status-related biochemical parameters were determined; the malondialdehyde level was increased, and anti-oxidative indices (including catalase, superoxide dismutase, glutathione, glutathione-S-transferase, and total antioxidant capacity) were decreased in the L22 group, which also induced DNA damage and inhibited the expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) pathway-related antioxidant genes to suppress antioxidant ability. …”
Get full text

Shock Wave Therapy Enhances Mitochondrial Delivery into Target Cells and Protects against Acute Respiratory Distress Syndrome by Kun-Chen Lin, Christopher Glenn Wallace, Tsung-Cheng Yin, Pei-Hsun Sung, Kuan-Hung Chen, Hung-I Lu, Han-Tan Chai, Chih-Hung Chen, Yi-Ling Chen, Yi-Chen Li, Pei-Lin Shao, Mel S. Lee, Jiunn-Jye Sheu, Hon-Kan Yip

“…The same profile was also seen for fibrosis/collagen deposition, levels of biomarkers of oxidative stress (NOX-1/NOX-2/oxidized protein), inflammation (MMP-9/TNF-α/NF-κB/IL-1β/ICAM-1), apoptosis (cleaved caspase 3/PARP), fibrosis (Smad3/TGF-β), mitochondrial damage (cytosolic cytochrome c) (all p<0.0001), and DNA damage (γ-H2AX+), and numbers of parenchymal inflammatory cells (CD11+/CD14+/CD40L+/F4/80+) (p<0.0001). …”
Get full text

Sangre Por Salud (SPS) Biobank: cohort profile by Richard R Sharp, Euijung Ryu, Janet E Olson, Karen Meagher, James R Cerhan, Davinder Singh, Suzette Bielinski, Emmil Zarrugh, Marlene E Girardo, Giovanna Moreno Garzon, Valentina Hernandez, Sharon Levey, Crystal Gonzalez, Daniela B Pompa, Gabriel Q Shaibi, Lawrence Mandarino, Steven Thibodeau, Elena De Filippis

“…In addition, participants had serum, plasma and DNA samples isolated and stored at the Mayo Clinic Arizona Biorepository Laboratory for long-term storage and future access. …”
Get full text

A two‐phase epigenome‐wide four‐way gene–smoking interaction study of overall survival for early‐stage non‐small cell lung cancer by Leyi Chen, Xiang Wang, Ning Xie, Zhongwen Zhang, Xiaowen Xu, Maojie Xue, Yuqing Yang, Liya Liu, Li Su, Maria Bjaanæs, Anna Karlsson, Maria Planck, Johan Staaf, Åslaug Helland, Manel Esteller, David C. Christiani, Feng Chen, Ruyang Zhang

“…Our previous work has identified a three‐way interaction among pack‐year of smoking (the number of packs of cigarettes smoked per day multiplied by the number of years the person has smoked) and two DNA methylation probes (cg05293407TRIM27 and cg00060500KIAA0226). …”
Get full text

Rapid and specific on-site H5Nx avian influenza diagnosis via RPA and PAM-independent CRISPR-Cas12a assay combined with anti-NP antibody-based viral RNA purification by Jin-Ha Song, Seung-Eun Son, Ho-Won Kim, Seung-Ji Kim, Se-Hee An, Chung-Young Lee, Hyuk-Joon Kwon, Hyuk-Joon Kwon, Hyuk-Joon Kwon, Hyuk-Joon Kwon, Kang-Seuk Choi, Kang-Seuk Choi

“…Additionally, by denaturing the RT-RPA amplicon using 60% DMSO, we activate the trans-ssDNA cleavage activity of the Cas12a protein without the need for a specific PAM (5’-TTTV-3′) sequence. …”
Get full text

Study of the hereditary non-syndromic ophthalmological pathology of child population of the Karachay-Cherkess Republic: estimations of genetic load and molecular genetic analysis by V.V. Kadyshev, A.V. Marakhonov, S.I. Kutsev, R.A. Zinchenko

“…<br> <br> <b>Key words:</b> epidemiology, genetics, genetic load, DNA diagnosis, children, Karachay-Cherkess Republic.…”
Get full text

Suppressing recurrence in Sonic Hedgehog subgroup medulloblastoma using the OLIG2 inhibitor CT-179 by Yuchen Li, Chaemin Lim, Taylor Dismuke, Daniel S. Malawsky, Sho Oasa, Zara C. Bruce, Carolin Offenhäuser, Ulrich Baumgartner, Rochelle C. J. D’Souza, Stacey L. Edwards, Juliet D. French, Lucy S. H. Ock, Sneha Nair, Haran Sivakumaran, Lachlan Harris, Andrey P. Tikunov, Duhyeong Hwang, Coral Del Mar Alicea Pauneto, Mellissa Maybury, Timothy Hassall, Brandon Wainwright, Santosh Kesari, Gregory Stein, Michael Piper, Terrance G. Johns, Marina Sokolsky-Papkov, Lars Terenius, Vladana Vukojević, Leon F. McSwain, Timothy R. Gershon, Bryan W. Day

“…We find that CT-179 disrupts OLIG2 dimerization, phosphorylation and DNA binding and alters tumor cell-cycle kinetics, increasing differentiation and apoptosis. …”
Get full text

A low-protein soybean-free diet improves carcass traits and meat quality and modulates the colonic microbiota in Daweizi pigs by Haohua Fu, Taoming Yang, Hengjia Ni, Hengjia Ni, Jing Li, Fenfen Liu, Jingbo Liu, Yulong Yin, Yulong Yin

“…Moreover, the purine content in the meat substantially decreased with the LPNS diet. The 16S rDNA analysis revealed that the LPNS diet greatly modified the composition of the colonic microbiota community, with a decrease in the Firmicutes/Bacteroidetes ratio and an increase in the abundance of Lactobacillus spp.DiscussionThe use of functional herbs along with a low-protein diet helped to regulate fat and purine metabolism in fatty-type pigs. …”
Get full text

29 m6A-RNA Methylation (Epitranscriptomic) Regulators Are Regulated in 41 Diseases including Atherosclerosis and Tumors Potentially via ROS Regulation – 102 Transcriptomic Dataset... by Ming Liu, Keman Xu, Fatma Saaoud, Ying Shao, Ruijing Zhang, Yifan Lu, Yu Sun, Charles Drummer, Li Li, Sheng Wu, Satya P. Kunapuli, Gerard J. Criner, Jianxin Sun, Huimin Shan, Xiaohua Jiang, Hong Wang, Xiaofeng Yang

“…We performed a database mining on 102 transcriptomic datasets for the expressions of 29 m6A-RNA methylation (epitranscriptomic) regulators (m6A-RMRs) in 41 diseases and cancers and made significant findings: (1) a few m6A-RMRs were upregulated; and most m6A-RMRs were downregulated in sepsis, acute respiratory distress syndrome, shock, and trauma; (2) half of 29 m6A-RMRs were downregulated in atherosclerosis; (3) inflammatory bowel disease and rheumatoid arthritis modulated m6A-RMRs more than lupus and psoriasis; (4) some organ failures shared eight upregulated m6A-RMRs; end-stage renal failure (ESRF) downregulated 85% of m6A-RMRs; (5) Middle-East respiratory syndrome coronavirus infections modulated m6A-RMRs the most among viral infections; (6) proinflammatory oxPAPC modulated m6A-RMRs more than DAMP stimulation including LPS and oxLDL; (7) upregulated m6A-RMRs were more than downregulated m6A-RMRs in cancer types; five types of cancers upregulated ≥10 m6A-RMRs; (8) proinflammatory M1 macrophages upregulated seven m6A-RMRs; (9) 86% of m6A-RMRs were differentially expressed in the six clusters of CD4+Foxp3+ immunosuppressive Treg, and 8 out of 12 Treg signatures regulated m6A-RMRs; (10) immune checkpoint receptors TIM3, TIGIT, PD-L2, and CTLA4 modulated m6A-RMRs, and inhibition of CD40 upregulated m6A-RMRs; (11) cytokines and interferons modulated m6A-RMRs; (12) NF-κB and JAK/STAT pathways upregulated more than downregulated m6A-RMRs whereas TP53, PTEN, and APC did the opposite; (13) methionine-homocysteine-methyl cycle enzyme Mthfd1 downregulated more than upregulated m6A-RMRs; (14) m6A writer RBM15 and one m6A eraser FTO, H3K4 methyltransferase MLL1, and DNA methyltransferase, DNMT1, regulated m6A-RMRs; and (15) 40 out of 165 ROS regulators were modulated by m6A eraser FTO and two m6A writers METTL3 and WTAP. …”
Get full text

<i>Ehrlichia</i> Species in Dromedary Camels (<i>Camelus dromedarius</i>) and Ruminants from Somalia by Aamir M. Osman, Ahmed A. Hassan-Kadle, Marcos R. André, Flávia C. M. Collere, Amir Salvador Alabí Córdova, Fabiano Montiani-Ferreira, Thállitha S. W. J. Vieira, Abdalla M. Ibrahim, Abdulkarim A. Yusuf, Rosangela Z. Machado, Rafael F. C. Vieira

“…This study aimed to investigate the molecular occurrence of <i>Ehrlichia</i> spp. in 530 animals (155 Dromedary camels, 199 goats, 131 cattle, and 45 sheep) in the Benadir and Lower Shabelle regions of Somalia. Blood DNA samples were tested for PCR targeting <i>dsb</i> and <i>sodB</i> genes of <i>Ehrlichia</i> spp. and <i>PCS20</i> and <i>map1</i> genes of <i>E. ruminantium</i>. …”
Get full text

Neospora caninum infection specifically suppresses the expression of a host lncRNA XR_001919077.1 to facilitate parasite propagation by modulating host cell mitochondrial function... by Shan-Shan Zhao, De-Liang Tao, Jin-Ming Chen, Ming-Yi Zhang, Xin Yang, Jun-Ke Song, Qun Liu, Guang-Hui Zhao

“…Rapid amplification of cDNA ends analysis generated six splice variants of XR_001919077.1, with lengths ranging from 592 to 694 nt. …”
Get full text

First detection and molecular characterization of Jingmen tick virus with a high occurrence in Rhipicephalus (Boophilus) microplus collected from livestock in Cameroon (2024) by Paloma Kiwan, Eva Lopez, Morena Gasparine, Geraldine Piorkowski, Agathe Colmant, Achille Paguem, Stephanie Mvodo, Laurence Thirion, Xavier de Lamballerie, Remi Charrel, Alessandra Falchi

“…Ticks were identified morphologically and molecularly. Total RNA/DNA was extracted from tick pools and screened for JMTV RNA using a segment 2 RT-qPCR system. …”
Get full text

Traditional pediatric massage enhanced the skeletal muscle mass in OVA-exposed adolescent rats via regulating SCFAs-FFAR2-IGF-1/AKT pathway by Lin Lin, Siyuan Li, Que Liu, Xingxing Zhang, Ying Xiong, Shaoyun Zhao, Liyue Cao, Jiaxuan Gong, Yaping Liu, Rong Wu

“…GM and SCFAs production were detected by 16S rDNA sequencing and GC-MS, respectively. The protein and gene expressions of free fatty acid receptor 2 in SM were detected by using immunohistochemical staining and qRT-PCR, respectively. qRT-PCR was used to detect other relative gene expressions that were closely related with SM mass. …”
Get full text