Major facility superfamily sugar transporter protein SsMFSST1 regulates Sporisorium scitamineum mating, pathogenicity, and sugar transport/absorption by Yi Zhang, Yongding He, Meixin Yan, Shilong Zhang, Sisi Zhou, Wankuan Shen

“…On YePSA medium, the gene SsMFSST1 does not affect the growth, spore morphology, colony morphology, and oxidative stress ability of the haploid spores of S. scitamineum. …”
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Inhibitory effects of limonin on the proliferation of non-small cell lung cancer cells via targeting S-phase kinase-associated protein 2 by Zhu Jiaxian, Zhang Mengting, Li Shengqing, Liu Yongqiang

“…Then, flow cytometry was used to detect the cell cycle and apoptosis, while colony formation assay assessed the low-density proliferation ability of NSCLC cells. …”
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RBM47 promotes cell proliferation and immune evasion by upregulating PDIA6: a novel mechanism of pancreatic cancer progression by Yihui Ma, Enjie Liu, Huijie Fan, Chenfei Li, Pei Huang, Meiying Cui, Zhengyang Wang, Jing Zhou, Kuisheng Chen

“…Loss- and gain-of-function effects of RBM47 on cell proliferation, tumor growth, and immune evasion were determined by CCK-8, EdU incorporation, colony formation assays, the xenogeneic tumor model, and co-culture system of PC and NK-92 cells. …”
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Probiotic administration aggravates dextran sulfate sodium salt-induced inflammation and intestinal epithelium disruption in weaned pig by Kunhong Xie, Weidong Cai, Lingjie Li, Bing Yu, Yuheng Luo, Zhiqing Huang, Xiangbing Mao, Jie Yu, Ping Zheng, Hui Yan, Hua Li, Jun He

“…From 1 to 15 d, the CA and DA groups were orally fed with 1.0 × 1011 colony-forming units A. muciniphila per day, while the CON and DCON groups were received gastric infusion of anaerobic sterile saline per day. …”
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Clinically relevant orthotopic pancreatic cancer models for adoptive T cell transfer therapy by Gregory B. Lesinski, Chrystal M. Paulos, Maggie Phillips, Jacklyn Hammons, Isaac Karpovsky, Zaid Mahdi, Natalie K. Horvat, Megan M. Wyatt, Margaret A. Hall, Cameron J. Herting, Richard A. Moffitt

“…However, these models have limitations, including marked tumor variability across individual mice and the cost of colony maintenance.Methods Using flow cytometry and immunohistochemistry, we characterized the immunological features and trafficking patterns of adoptively transferred T cells in orthotopic PDAC (C57BL/6) models using two mouse cell lines, KPC-Luc and MT-5, isolated from C57BL/6 KPC-GEMM (KrasLSL-G12D/+p53−/− and KrasLSL-G12D/+p53LSL-R172H/+, respectively).Results The MT-5 orthotopic model best recapitulates the cellular and stromal features of the TME in the PDAC GEMM. …”
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Circular RNA RHOT1 Regulates miR-142-5p/CCND1 to Participate in Chondrocyte Autophagy and Proliferation in Osteoarthritis by Gu Man, Haiwen Yang, Kai Shen, Donghong Zhang, Julin Zhang, Hao Wu, Hongyu Zhang, Jinhuan Wang

“…The function of circRHOT1 in the regulation of OA was assessed by CCK-8, colony formation, flow cytometry analysis, quantitative real-time PCR, Western blot analysis, and luciferase reporter gene assay in chondrocytes. …”
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Mobilization of Stem Cells Using G-CSF for Acute Ischemic Stroke: A Randomized Controlled, Pilot Study by Kameshwar Prasad, Amit Kumar, Jitendra Kumar Sahu, M. V. P. Srivastava, Sujata Mohanty, Rohit Bhatia, Shailesh B. Gaikwad, Achal Srivastava, Vinay Goyal, Manjari Tripathi, Chandrashekar Bal, Nalini Kant Mishra

“…There is emerging evidence to support the use of granulocyte colony-stimulating factor (G-CSF) therapy in patients with acute ischemic stroke. …”
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Parishin B blocking TRIB3-AKT1 interaction inhibits breast cancer lung metastasis by Xiongtao Cheng, Xiongtao Cheng, Jianguo Sun, Shouhong Chen, Nan Wang, Weijing Tang, Zijian Xia, Yuhong Shu, Shouhong Gao, Zhipeng Wang, Xinxia Wang, Rongzi Shao, Jianxiong Cao, Jianxiong Cao

“…Then, the effect of TRIB3 inhibitor anti BC was assessed by CCK-8 assay, flow cytometry, plate colony formation assay, and transwell assay; and the RNA-seq was empolyed to study the potential mechanism of Parishin B (PB) anti-BC. …”
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Melatonin Reverses the Loss of Stemness Induced by TNF-α in Human Bone Marrow Mesenchymal Stem Cells through Upregulation of YAP Expression by Xudong Wang, Tongzhou Liang, Jincheng Qiu, Xianjian Qiu, Bo Gao, Wenjie Gao, Chengjie Lian, Taiqiu Chen, Yuanxin Zhu, Anjing Liang, Peiqiang Su, Yan Peng, Dongsheng Huang

“…In this study, we report a melatonin-based method to reduce the inflammatory effects on the stemness of bone marrow mesenchymal stem cells (BMMSCs). The results of colony formation assays, Alizarin red staining, western blotting, and reverse transcription-polymerase chain reactions suggest that melatonin can reverse the inflammatory damage caused by TNF-α treatment in the third, seventh, and tenth generations of primary BMMSCs (vs. control and the TNF-α-treated group). …”
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The effect of GM-CSF and predictors of treatment outcome in pediatric septic shock patients by Zhen-Hao Yu, Gui-Xiang Tian, Yao-Dong Wang, Ting-Yan Liu, Peng Shi, Jia-Yun Ying, Wei-Ming Chen, Yu-Feng Zhou, Guo-Ping Lu, Cai-Yan Zhang

“…Granulocyte-macrophage colony-stimulating factor (GM-CSF) has been explored as a therapeutic intervention to counteract this immunosuppression. …”
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USP39 phase separates into the nucleolus and drives lung adenocarcinoma progression by promoting GLI1 expression by Shaoxuan Cheng, Zhiyuan Qiu, Ziyi Zhang, Yuxuan Li, Yue Zhu, Yuxin Zhou, Yinghui Yang, Yaowen Zhang, Dian Yang, Yingqiu Zhang, Han Liu, Zhaoxia Dai, Shu-Lan Sun, Shuyan Liu

“…The effects of USP39 depletion on tumor progression were tested using doxycycline-inducible USP39 knockdown and rescue lung adenocarcinoma cells both in vitro and in vivo by performing MTT, colony formation, EdU staining, transwell and tumor xenograft model experiments. …”
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Immunogenicity in humans of a transdermal multipeptide melanoma vaccine administered with or without a TLR7 agonist by Craig L Slingluff, Gina R Petroni, Walter C Olson, Kimberly A Chianese-bullock, Donna Deacon, Mark Smolkin, William W Grosh, Max O Meneveau, Kevin T Lynch, Elise P Salerno, Elizabeth Woodson, James W Patterson

“…We hypothesized that: (1) applying melanoma-derived peptides topically, in proximity to LC, could be immunogenic and safe, with low vaccine-site toxicity and (2) topical toll-like receptor 7 (TLR7) agonist would increase immunogenicity of the peptide vaccine.Methods Twelve melanoma peptides plus a tetanus helper peptide were combined with granulocyte macrophage colony stimulating factor (GM-CSF) and were administered topically on days 1, 8, and 15, to 28 patients randomized to one of four adjuvant preparations: (1) incomplete Freund’s adjuvant (IFA); (2) IFA plus a TLR7 agonist (imiquimod) administered on days 0, 7, 14; (3) dimethyl sulfoxide (DMSO) or (4) DMSO+ imiquimod administered on day 0, 7, 14. …”
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MSCs with upregulated lipid metabolism block hematopoietic stem cell differentiation via exosomal CTP-1A in MDS by Chunlai Yin, Xue Yan, Jinyi Ren, Cheng Zhang, Jiaqing Liu, Zilong Wang, Jing Liu, Weiping Li, Xia Li

“…The proliferation, apoptosis, differentiation and support for hematopoiesis of these cells were analyzed using CCK-8 assay, FC and induction medium and CFU (colony forming units) assay, respectively. Expression levels of metabolic molecules were used as indicators to screen MSCs with different metabolic pathways and were detected by RT-PCR and Western blotting. …”
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Identification of cancer cell-intrinsic biomarkers associated with tumor progression and characterization of SFTA3 as a tumor suppressor in lung adenocarcinomas by Yu Zhao, Chengcheng Zhou, Ling Zuo, Haoming Yan, Yuhan Gu, Hong Liu, Guiping Yu, Xiaorong Zhou

“…Furthermore, a series of biological experiments, including cell viability assays, scratch wound healing assays, and colony formation assays, were conducted to demonstrate the tumor-suppressive effects of SFTA3. …”
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Relationship between CTF1 gene expression and prognosis and tumor immune microenvironment in glioma by Hongqing Cai, Shen Tian, Angsi Liu, Guanchao Xie, Hongsheng Zhang, Xiaogang Wu, Jinghai Wan, Sai Li

“…To assess the impact on glioma cell proliferation and apoptosis, we employed various assays, including the Cell Counting Kit-8, colony formation assay, and flow cytometry. Results CTF1 gene and protein expression were significantly elevated in glioma tissues, and correlated with malignancy and poor prognosis. …”
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Eicosapentaenoic Acid and Docosahexaenoic Acid as an Antimicrobial Agent in Orthopedics—An In Vitro Study About the Race for Surface by Christopher Spiegel, Burak Ünalan, Andreas Kaserbacher, Rohit Arora, Débora C. Coraça-Huber

“…After establishing the co-culture, cell culture and biofilm, we performed colony-forming unit counting and cell counting to examine cell survivability. …”
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Bacteriophage Treatment Induces Phenotype Switching and Alters Antibiotic Resistance of ESBL <i>Escherichia coli</i> by Árpád Rózsa, László Orosz, Nikoletta Szemerédi, Gabriella Spengler, Gábor Kecskeméti, Otília Vágó, Károly Péter Sárvári, Diana Szabó, Zoltán Szabó, Katalin Burián, Dezső Péter Virok

“…<b>Results:</b> The bacteriophage infection led to notable alterations in colony morphology, indicating profound changes in bacterial metabolism. …”
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A Bayesian analysis of birth pulse effects on the probability of detecting Ebola virus in fruit bats by Pleydell, David R.J., Ndong Bass, Innocent, Mba Djondzo, Flaubert Auguste, Meta Djomsi, Dowbiss, Kouanfack, Charles, Peeters, Martine, Cappelle, Julien

“…The current study presents an age-structured epidemiological model that characterises the effects of seasonal birth pulses on ebolavirus transmission within a colony of African straw-coloured fruit bats (Eidolon helvum). …”
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Vertical Transmission of Group B Streptococcus, Prevalence, Associated Factors, and Antimicrobial Susceptibility Profile among Newborns Delivered at Health Facilities in Jigjiga Ci... by Addisu Tesfaye, Mahlet Fekede, Fasil Getu, Surafel Mekuria, Tigist Abebe, Daniel Tollosa, Shamil Barsenga, Bawlah Tahir, Abdurahman Kedir Roble, Sara Tesfaye, Muluken Walle

“…Samples were inoculated on Todd Hewitt broth media supplemented with gentamycin and nalidixic acid and then subcultured on blood agar. Colony characteristics, Gram stain, and catalase test were used for identification. …”
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Hyperbaric oxygen potentiates platelet-rich plasma composition and accelerates bone healing by Wen-Shuo Chang, Chien-Cheng Huang, Tzu-Hao Chen, Ssu-Han Chao, Cheng-Hsien Lin, Ching-Ping Chang, Chi-Sheng Chien

“…Results: hPRP showed significantly higher concentrations of growth factors such as activin-A, brain-derived neurotrophic factor, nerve growth factor, Flt-3 Ligand, granulocyte-macrophage colony-stimulating factor, hepatocyte growth factor, and platelet-derived growth factor, compared to nPRP. …”
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