Development of a live cell assay for real-time monitoring the interactions between the Hippo pathway components 14-3-3 and TAZ by Blaž Andlovic, Alexander Wolf, Malgorzata Hiltmann, Bert M. Klebl, Jan Eickhoff, Christian Ottmann

Subjects: “…Cell-based assay…”
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Quantitative analysis of the effect of microtubule-targeting drugs on the microtubule cytoskeleton of breast cancer cells with different invasive properties by Michallet, Sophie, Bosc, Lauriane, Lafanechère, Laurence

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Protocol to calculate the synergy of drug combinations in organoids and primary cells from murine tumors by David Eisenbarth, Bomin Ku, Dae-Sik Lim

Subjects: “…Cell-based Assays…”
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Protocol for assessing T cell receptor-mediated human T cell cytotoxicity by Kathryne E. Marks, Ifeoluwakiisi Adejoorin, John Sowerby, Deepak A. Rao

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Protocol to study monocyte transmigration across primary human liver endothelial cells under physiological shear flow conditions in vitro by Alex L. Wilkinson, Megan E. Bannister, Ayla O’Keeffe, Chris J. Weston, Patricia F. Lalor, Shishir Shetty, Daniel A. Patten

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Protocol for detecting homologous recombination activity in cancer cells by adenovirus-based functional assay by Shih-Han Huang, Chih-Ying Lee, Peter Chi

Subjects: “…cell-based assays…”
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Protocol for CRISPR-Cas9 genome editing of a swine cell line via electroporation by Patricia Kiesler, Stella S. Lee, Alexis L. Norris, Mayumi F. Miller, Carlo J. Mercado, Adam L. Moyer, Samantha Maragh

Subjects: “…Cell-based Assays…”
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Protocol for assessing immune-target cell interactions using a single-cell cytotoxicity assay by Zhihao Wei, Konglan Lin, Min Huang, Shicheng Su, Yiwen Lu

Subjects: “…Cell-based Assays…”
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Protocol to identify small-molecule inhibitors against cancer drug resistance by Juan Wei, She-Yu Zhang, Xue-Yuan Zhou, Yong Wei, Hao-Ran Jia, Qin Wu, Weihong Tan

Subjects: “…cell-based assays…”
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Protocol for odorant-binding protein 2A gene knockdown by dual siRNA transfection in a three-dimensional human epidermal equivalent model by Shinobu Nakanishi

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Protocol to measure and analyze protein interactions in mammalian cells using bioluminescence resonance energy transfer by Carla Jane Duval, Candy Laura Steffen, Karolina Pavic, Daniel Kwaku Abankwa

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Protocol to identify receptors of secreted proteins through CRISPR-Cas9 whole-genome screening technology by Xiaoli Hu, Yiguo Wang

Subjects: “…Cell-based Assays…”
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Protocol for live neuron imaging analysis of basal surface fraction and dynamic availability of the dopamine transporter using DAT-pHluorin by Elnaz Khezerlou, Jacqueline Saenz, Sanjana Surya Prakash, Ping-Yue Pan

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Protocol for extracellular vesicle secretion-related gene screening via ExoScreen technique by Tomofumi Yamamoto, Fumihiko Urabe, Yusuke Yoshioka, Yusuke Yamamoto, Takahiro Ochiya

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Protocol for oil red O staining of low-density lipoproteins for in vivo cell treatment by Simone Bini, Stella Covino, Ilenia Minicocci, Laura D’Erasmo, Daniele Tramontano, Alessia Di Costanzo, Marcello Arca, Valeria Pecce

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Click-chemistry-based protocol for detecting 4-octyl-itaconate-alkylated proteins in primary mouse macrophages by Chaofei Su, Tian Cheng, Hanyi Zhang, Hang Yin

Subjects: “…Cell-based Assays…”
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Protocol for whole-cell patch-clamp assay for HBV-mediated sodium influx in THP-1 macrophages by Yingbao Tan, Penghui Chen, Xiaojuan Xin, Jingxue Wang

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Protocol to detect neutral lipids with BODIPY staining in myeloid-derived suppressor cells in mouse mammary tumors by Ukjin Kim, Rumela Chakrabarti

Subjects: “…Cell-based Assays…”
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Protocol to identify E3 ligases amenable to biodegraders using a cell-based screening by Marie Sorbara, Margot Cristol, Anaïs Cornebois, Klervi Desrumeaux, Pierre Cordelier, Nicolas Bery

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Protocol for a patient-derived preclinical platform to model tumor-immune interactions and evaluate therapeutic efficacy by Wen-Hsin Chang, Andrew I. Chin, Ching-Hsien Chen

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