Postimplantation Whole Embryo Culture Assay for Hamsters: An Alternative to Rat and Mouse by Bogdan Wlodarczyk, Bogumil Biernacki, Maria Minta, Jan Zmudzki

“…Postimplantation whole embryo culture (WEC) assay for rats and mice has been well established and introduced to many laboratories. …”
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Toxicity Evaluation of Sulfobetainized Branched Polyethyleneimine via Antibacterial and Biocompatibility Assays by Mehtap Sahiner, Selin S. Suner, Sahin Demirci, Ramesh S. Ayyala, Nurettin Sahiner

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Pharmaceutical Development of «Sedoflav» Capsules, Standardization and Validation of Flavonoids Assay Methods by M. A. Dzhavakhyan, M. G. Tokareva, Yu. E. Prozhogina, E. I. Kalenikova

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Dye‐based recombinase‐aided amplification assay with enhanced sensitivity and specificity by Zijin Zhao, Yanbo You, Shaowei Hua, Xinxin Shen, Lingjun Li, Xuejun Ma

“…Abstract Background Fluorescent recombinase‐aided amplification (RAA) assays are increasingly being used in the detection of a variety of pathogens and have the advantages of rapidity and simplicity and similar sensitivity and specificity, compared with real‐time PCR (qPCR) assays, but they require a complex probe design. …”
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Thyrotoxicosis antidote assay along with concurrent medication; chromatographic and environmental issues by Raghda A. Emam, Aml A. Emam, Rehab M. Abdelfatah, Basma H. Anwar

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A novel luciferase-based assay for quantifying coronavirus-induced syncytia by Keisuke Oguma, Kenji Ogawa

“…This assay, which is based on the split luciferase system, utilizes a split Gaussia luciferase (Gluc) system. …”
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Potentials of cytokinesis blocked micronucleus assay in radiation triage and biological dosimetry by G. Tamizh Selvan, P. Venkatachalam

“…The cytokinesis blocked micronucleus (CBMN) assay is the alternate for the gold standard dicentric chromosome assay in radiation dose assessment. …”
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Development of Immunocapture-LC/MS Assay for Simultaneous ADA Isotyping and Semiquantitation by Lin-Zhi Chen, David Roos, Elsy Philip

“…Enzyme-linked immunosorbent assays and electrochemiluminescence immunoassays are the most widely used platform for ADA detection due to their high sensitivity and throughput. …”
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A highly multiplexed quantitative phosphosite assay for biology and preclinical studies by Hasmik Keshishian, E Robert McDonald, Filip Mundt, Randy Melanson, Karsten Krug, Dale A Porter, Luke Wallace, Dominique Forestier, Bokang Rabasha, Sara E Marlow, Judit Jane‐Valbuena, Ellen Todres, Harrison Specht, Margaret Lea Robinson, Pierre M Jean Beltran, Ozgun Babur, Meagan E Olive, Javad Golji, Eric Kuhn, Michael Burgess, Melanie A MacMullan, Tomas Rejtar, Karen Wang, DR Mani, Shankha Satpathy, Michael A Gillette, William R Sellers, Steven A Carr

“…Here, we present SigPath, a targeted mass spectrometry (MS) assay that measures 284 phosphosites in 200 phosphoproteins of biological interest. …”
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Microhaplotype deep sequencing assays to capture Plasmodium vivax infection lineages by Mariana Kleinecke, Edwin Sutanto, Angela Rumaseb, Kian Soon Hoon, Hidayat Trimarsanto, Ashley Osborne, Paulo Manrique, Trent Peters, David Hawkes, Ernest Diez Benavente, Georgia Whitton, Sasha V. Siegel, Richard D. Pearson, Roberto Amato, Anjana Rai, Nguyen Thanh Thuy Nhien, Hoang Chau Nguyen, Ashenafi Assefa, Tamiru S. Degaga, Dagimawie Tadesse Abate, Awab Ghulam Rahim, Ayodhia Pitaloka Pasaribu, Inge Sutanto, Mohammad Shafiul Alam, Zuleima Pava, Tatiana Lopera-Mesa, Diego Echeverry, Tim William, Nicholas M. Anstey, Matthew J. Grigg, Nicholas P. Day, Nicholas J. White, Dominic P. Kwiatkowski, Aimee R. Taylor, Rintis Noviyanti, Daniel Neafsey, Ric N. Price, Sarah Auburn

“…We developed a high-throughput amplicon sequencing assay comprising 93 multi-SNP (microhaplotype) markers to determine IBD between P. vivax clinical isolates. …”
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Chromogranin B Purification for Condensate Formation and Client Partitioning Assays In Vitro by Anup Parchure, Julia von Blume

“…Here, we describe a protocol to purify the tagged version of chromogranin B close to its native form at the TGN, which can then be utilized for microscopy-based assays to monitor condensate formation in vitro and client partitioning depending on the material properties of chromogranin B assemblies.…”
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Vital Stain to Study Cell Invasion in Modified Boyden Chamber Assay by Madhura R. Vipra, Jayant M. Chiplonkar

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Optimizing Microneutralization and IFN-γ ELISPOT Assays to Evaluate Mpox Immunity by Yinyi Yu, Krystal Meza, Chase Colbert, Daniel F. Hoft, Anna Jaunarajs, Azra Blazevic, Sharon E. Frey, Getahun Abate

“…Background: Available assays to measure pox virus neutralizing antibody titers are laborious and take up to 5 days. …”
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Development of a droplet digital PCR assay for the detection of BK polyomavirus by Lu Ai, Yating Zhao, Chianru Tan, Lu Bai, Gang Huang, Ruizhi Wang, Hao Huang, Xuegao Yu, Yong Guo, Peisong Chen

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Evaluating old and novel proxies for in vitro digestion assays in wild ruminants by Pierre‐Olivier Jean, Robert L. Bradley, Robert Berthiaume, Jean‐Pierre Tremblay

“…ABSTRACT In vitro digestion assays provide useful data for wild ruminant ecologists. …”
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Automated whole animal bio-imaging assay for human cancer dissemination. by Veerander P S Ghotra, Shuning He, Hans de Bont, Wietske van der Ent, Herman P Spaink, Bob van de Water, B Ewa Snaar-Jagalska, Erik H J Danen

“…This automated quantitative whole animal bio-imaging assay can serve as a first-line in vivo screening step in the anti-cancer drug target discovery pipeline.…”
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Comparison of Methods for Quantitation of Radioactivity in Protected Hybrids in RNase Protection Assays by Kara Pham, K. Steven LaForge, Mary Jeanne Kreek

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A single entomopathogenic nematode infection assay for Drosophila melanogaster larvae by Sreeradha Mallick, Eric Kenney, Jacob Rashap, Ioannis Eleftherianos

Subjects: “…Nematode infection assay…”
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Optimization of human TAT-Fab antibody expression and its bioactivity assay by WANG Qun, LIU Jun-li, GAO Fei, ZHANG Pin, ZHANG Li-ning

“…In order to establish high effective expression system for human TAT-Fab antibody with protective activity, the induction conditions for the expression of TAT-Fab antibody in E. coli were optimized, and the bioactivity of antibody was assayed in vitro and in vivo. ELISA showed that human TAT-Fab antibody expressed in E. coli possessed binding activity with tetanus toxin, and the expression level increased to nearly 5 times as compared with that before optimization. …”
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Comparison of four serological assays for the diagnosis of Chlamydia trachomatis in subfertile women by Claude Muvunyi, Laurens Claeys, Tineka De Sutter, Petra De Sutter, Marleen Temmerman, Lieve Van Renterghem, Geert Claeys, Elizaveta Padalko

“…Results: A comparison of the serological assays showed  little difference  in the major performance characteristics: the sensitivities of all MIFs and ELISAs were 100% for all assays (except the ELISA Vircell, with a sensitivity of 90%), and the specificities ranged from 92% for MIF Ani Labsystems to 98% for the MIF Focus and ELISA Vircell. …”
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