Genome-wide analysis and characterization of TPD1 family proteins in pearl millet (Cenchrus americanus): Insights into reproductive regulation and phytohormone responses. by Zainab M Almutairi

“…The phylogenetic tree revealed distinct clustering of CaTPD1_Ch6 and CaTPD1_Ch5 among the other CaTPD1s, which revealed close relationships with the orthologs from Arabidopsis and rice that are known to have a critical role in tapetum development and pollen and ovule production. …”
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Multiplexed transcriptomic analyzes of the plant embryonic hourglass by Hao Wu, Ruqiang Zhang, Karl J. Niklas, Michael J. Scanlon

“…Transcriptomic comparisons of maize and Arabidopsis embryogenesis with that of the moss Physcomitrium patens identify an inverse hourglass pattern across plant phyla, as in animals. …”
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An Improved Quantitative Analysis Method for Plant Cortical Microtubules by Yi Lu, Chenyang Huang, Jia Wang, Peng Shang

“…Meanwhile, in order to further verify its reliability, the proposed texture analysis method was utilized to distinguish different images of Arabidopsis microtubules. The results showed that the effect of BEMD algorithm on edge preserving accompanied with noise reduction was positive, and the geometrical characteristic of the texture was obvious. …”
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Measuring plant cysteine oxidase interactions with substrates using intrinsic tryptophan fluorescence by Dona M. Gunawardana, Daisy A. Southern, Emily Flashman

“…Here, we report development of an assay that exploits the intrinsic fluorescence of Arabidopsis thaliana PCO tryptophan residues. By using Ni(II)-substitued enzymes and preparing the assay under anaerobic conditions, tryptophan fluorescence quenching is observed on enzyme:substrate complex formation, allowing quantification of binding affinities. …”
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Transcriptome-Based Analysis of Dof Family Transcription Factors and Their Responses to Abiotic Stress in Tea Plant (Camellia sinensis) by Hui Li, Wei Huang, Zhi-Wei Liu, Yong-Xin Wang, Jing Zhuang

“…The 29 CsDof proteins were divided into 7 groups (A, B1, B2, C1, C2.1, C2.2, and D2), and the interaction networks of Dof proteins in C. sinensis were established according to the data in Arabidopsis. Gene expression was analyzed in “Yingshuang” and “Huangjinya” under four experimental stresses by qRT-PCR. …”
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Development and application of sugarcane yellow leaf virus vectors in sugarcane by Yuteng Yin, Pingzhi Zhao, Yanwei Sun, Tian Han, Mengrong Wang, Meixin Yan, Jianming Wu, Hui Zhou, Jian Ye

“…In this study, we present the development of the first infectious cDNA clone of SCYLV, which could efficiently infect Nicotiana benthamiana, Arabidopsis thaliana and sugarcane plants. SCYLV-based recombinant heterologous EGFP protein vector was also developed. …”
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MODELING OF PLANT EMBRYO MORPHODYNAMICS AT EARLY DEVELOPMENTAL STAGES by S. V. Nikolaev, N. A. Kolchanov, S. K. Golushko, J.-C. Palauqui, A. Urban, E. V. Amelina, A. V. Yurchenko, K. S. Golushko, U. S. Zubairova, A. V. Penenko, A. Trubuil

“…Embryo morphodynamics at early developmental stages of Arabidopsis thaliana was studied. First, a pipeline was elaborated from confocal microscopy and tissue 3D reconstruction to cell lineage tree reconstruction and numerical simulation of growing embryo mechanics. …”
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The Populus MYB transcription factor PSAR1 negatively regulates salt stress and is a repressor of ABA signaling by Qing Fang, Caiyun Zhang, Jingjie Liu, Zhi Zeng, Yanke Lu

“…A markedly diminished tolerance to salt stress was observed in the engineered Arabidopsis overexpressing PSAR1 (OeLAs) compared with Wt (wild-type) plants. …”
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DFMA: an improved DeepLabv3+ based on FasterNet, multi-receptive field, and attention mechanism for high-throughput phenotyping of seedlings by Liangquan Jia, Tao Wang, Xiangge Li, Lu Gao, Qiangguo Yu, Xincheng Zhang, Shanlin Ma

“…On publicly available datasets, including Arabidopsis thaliana, Brachypodium distachyon, and Sinapis alba, detection scores reached 87.69%, 91.07%, and 66.44%, respectively, outperforming existing models. …”
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Halo-RPD: searching for RNA-binding protein targets in plants by A. O. Shamustakimova

“…The proposed protocol uses plants with stable fusion protein expression and Magne® HaloTag® magnetic beads to capture RNA-protein complexes directly from the cytoplasmic lysate of transgenic Arabidopsis thaliana plants. The key stages described in the paper are as follows: (1) preparation of the magnetic beads; (2) tissue homogenization and collection of control samples; (3) precipitation and wash of RNA-protein complexes; (4) evaluation of protein binding efficiency; (5) RNA isolation; (6) analysis of the RNA obtained. …”
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Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism by Yihua Zhan, Jing Wang, Xuan Zhao, Zhifu Zheng, Yi Gan

“…Results Herein, AhMAGL3b encoding a protein with both hydrolase and acyltransferase regions, a member of MAGL gene family, was cloned and overexpressed in Arabidopsis thaliana. A total of 9 homozygous T3 generation transgenic lines were obtained. …”
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Development of robust constitutive synthetic promoter using genetic resources of plant pararetroviruses by Tsheten Sherpa, Tsheten Sherpa, Nrisingha Dey

“…Finally, we tested the efficacy and tissue specificity of the MFH17 promoter in planta by developing transgenic Nicotiana tabacum and Arabidopsis thaliana plants and found it highly constitutive and efficient in driving the gene throughout the plant tissues. …”
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A multiprotein regulatory module, MED16–MBR1&2, controls MED25 homeostasis during jasmonate signaling by Fangming Wu, Chuanlong Sun, Ziying Zhu, Lei Deng, Feifei Yu, Qi Xie, Chuanyou Li

“…Here, we report that Arabidopsis MED16 activates JA-responsive gene expression by promoting MED25 stability. …”
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Comparative Evaluation of Intron Prediction Methods and Detection of Plant Genome Annotation Using Intron Length Distributions by Long Yang, Hwan-Gue Cho

“…Using two model plant (rice and Arabidopsis) genomes, we compared two well-known intron prediction tools: the Blast-Like Alignment Tool (BLAT) and Sim4cc. …”
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Brassica rapa selenium transporter NPF2.20 (BrNPF2.20) accounts for Se-enrichment in Chinese cabbage by Xiaoting Hu, Yucheng Chen, Weihong Xu

“…The overexpression of BrNPF2.20 significantly increased the Se content in Arabidopsis plants, whereas silencing BrNPF2.20 in Chinese cabbage leaves reduced the Se content. …”
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Progress in plant genome sequencing: research directions by M. K. Bragina, D. A. Afonnikov, E. A. Salina

“…Since the first plant genome of Arabidopsis thaliana has been sequenced and published, genome sequencing technologies have undergone significant changes. …”
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Pseudomonas evades immune recognition of flagellin in both mammals and plants. by Bart W Bardoel, Sjoerd van der Ent, Michiel J C Pel, Jan Tommassen, Corné M J Pieterse, Kok P M van Kessel, Jos A G van Strijp

“…Interestingly, AprA also prevents flagellin-mediated immune responses (such as growth inhibition and callose deposition) in Arabidopsis thaliana plants. This was due to decreased activation of the receptor FLS2 and clearly demonstrated by delayed stomatal closure with live bacteria in plants. …”
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Pea (Pisum sativum L.): the uneasy fate of the first genetical object by O. E. Kosterin

“…Pea generation time can be shortened to 35 days, which is comparable with Arabidopsis. However, small and hardly recognizable chromosomes hampered the development of pea cytogenetics, while recombination genetic maps remained inadequate until 1990s, when they were improved only with the aid of molecular methods. …”
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Immunofluorescence for Detection of TOR Kinase Activity In Situ in Photosynthetic Organisms by Ana Lando, María De Marco, Andrea Cumino, Giselle Martínez-Noël

“…Here, we describe an in vivo protocol to detect S6K phosphorylation in tissues/cells of model photosynthetic organisms such as Arabidopsis thaliana and Chlamydomonas reinhardtii. …”
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Functional Characterization of Sesquiterpene Synthase from Polygonum minus by Su-Fang Ee, Zeti-Azura Mohamed-Hussein, Roohaida Othman, Noor Azmi Shaharuddin, Ismanizan Ismail, Zamri Zainal

“…To examine the function of PmSTS, we expressed this gene in Arabidopsis thaliana. Two transgenic lines, designated as OE3 and OE7, were further characterized, both molecularly and functionally. …”
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