Optimization of Agrobacterium tumefaciens-Mediated Immature Embryo Transformation System and Transformation of Glyphosate-Resistant Gene 2mG2-EPSPS in Maize (Zea mays L.) by Gui-rong YU, Yan LIU, Wen-ping DU, Jun SONG, Min LIN, Li-yuan XU, Fang-ming XIAO, Yong-sheng LIU

“…Using the optimized transformation procedure, more than 800 transgenic plants were obtained from 78599, Zong 31 and BA. …”
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An in vivo comparative evaluation of antimicrobial efficacy of chitosan, chlorhexidine gluconate gel and their combination as an intracanal medicament against Enterococcus faecalis... by A. Savitha, A. SriRekha, R. Vijay, Ashwija, C. Champa, T. Jaykumar

“…Aim: To evaluate the in vivo antibacterial efficacy of 2% chlorhexidine gel (CHX), 2% chitosan (CS) gel and their combination as an intracanal medicament against Enterococcus faecalis during endodontic retreatment procedure, with the use of qPCR. Method: A total of 45 single rooted permanent teeth were selected from 28 systemically healthy patients (mean age of 43 years). …”
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Coinfection of COVID-19 and malaria: clinical profiles, interactions, and strategies for effective control by Mu-Zi He, Hai-Ting Zhang, Yi Yang, Yi Fang, Mao Zhang, Sheng-Qun Deng, Xun Sun

“…The overlap in epidemiology, infection incubation, and clinical symptoms between COVID-19 and malaria coinfections has been thoroughly discussed to provide a detailed diagnostic procedure for coinfections, thereby guiding appropriate clinical interventions. …”
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A Rare Case of Monogenic Obesity Due to a Novel Variant in the ADCY3 Gene: Challenges in Follow-up and Treatment by Bahar Özcabı, Asude Durmaz, Ayça Aykut, Hasan Önal, Samim Özen

“…However, few patients with homozygous mutations have been described to date and the follow-up procedure and treatment options are unclear. A 10-month-old female presented with increased appetite and weight gain. …”
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A roadmap to implementing outpatient administration of bispecific antibodies in multiple myeloma by Alfred L. Garfall, Rahul Banerjee, Laurent Frenzel, Cyrus Khandanpour, Cyrus Khandanpour, Cyrus Khandanpour, Yi Lin, Erica Ottoni, Robert Rifkin, Sarah Rockwell, Cesar Rodriguez, Humberto Villefort, Elena Zamagni, Elena Zamagni

“…This report provides actionable guidance to implement safe and effective administration of BsAbs for patients with RRMM in outpatient and community settings.MethodsThree clinician advisory workshops were held in the United States, Europe, and Latin America to discuss key factors to operationalize BsAb use in outpatient and community settings, focusing on the critical phases of practice setup, treatment initiation, and ongoing management.ResultsBsAb administration in outpatient and community settings requires careful planning, a well-prepared multidisciplinary team (MDT) of healthcare professionals, and clear protocols, including MDT composition, roles/responsibilities, capacity planning, patient selection criteria, step-up dosing procedure, admission processes, patient/caregiver education requirements, and adverse event (AE) monitoring/management. …”
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MultiModal Ensemble Approach Leveraging Spatial, Skeletal, and Edge Features for Enhanced Bangla Sign Language Recognition by Khan Abrar Shams, Md. Rafid Reaz, Mohammad Ryan Ur Rafi, Sanjida Islam, Md. Shahriar Rahman, Rafeed Rahman, Md. Tanzim Reza, Mohammad Zavid Parvez, Subrata Chakraborty, Biswajeet Pradhan, Abdullah Alamri

“…To address this, we propose a procedure that reduces these uncertainties by considering three modalities: spatial information, skeleton awareness, and edge awareness. …”
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Importance of superficial circumflex iliac artery preservation to prevent abdominal wall necrosis in patients who underwent mini-abdominoplasty:... by Carlos Lacerda de Andrade Almeida, Luiz Felipe Duarte Fernandes Vieira, Luiz Alberto de Souza Leite, Jonathan Augusto Vidal de Oliveira, Rafael Figueiredo Ataide, Emanuel José Baptista Oliveira, Carlos Teixeira Brandt

“…INTRODUCTION: Abdominoplasty is the third most performed procedure in plastic surgery. To avoid surgical complications, a study of the superficial circumflex iliac artery of the abdomen (SCIA) was carried out to investigate the importance of this artery preservation in abdominoplasties as one of the high importance factors to prevent necrosis. …”
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Imaging and sentinel lymph node biopsy in high risk head and neck cutaneous squamous cell carcinoma: a Chinese cohort study by Yuedi Ma, Yuedi Ma, Yuedi Ma, Yuedi Ma, Dongqiu Shan, Haisan Zhang, Haisan Zhang, Haisan Zhang

“…ObjectiveTo evaluate the most effective modalities for detecting lymph node metastasis and to ascertain whether these procedures influenced management decisions and correlated with disease-related outcomes in head and neck cutaneous squamous cell carcinoma (HNcSCC) based on a Chinese cohort.MethodsHigh-risk HNcSCC patients were retrospectively enrolled and categorized into three groups based on neck evaluation methods: ultrasound (U), ultrasound plus CT (UC), and ultrasound plus CT plus sentinel lymph node biopsy (UCS). …”
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Combining different CRISPR nucleases for simultaneous knock-in and base editing prevents translocations in multiplex-edited CAR T cells by Viktor Glaser, Christian Flugel, Jonas Kath, Weijie Du, Vanessa Drosdek, Clemens Franke, Maik Stein, Axel Pruß, Michael Schmueck-Henneresse, Hans-Dieter Volk, Petra Reinke, Dimitrios L. Wagner

“…Conclusions We outline a solution for non-viral CAR gene transfer and efficient gene silencing using different CRISPR enzymes for knock-in and base editing to prevent translocations. This single-step procedure may enable safer multiplex-edited cell products and demonstrates a path towards off-the-shelf CAR therapeutics.…”
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Cut-off point estimation of Neck circumference to determine Overweight and Obesity among Asian Indian adults by Nitish Mondal, Ruplin Timungpi, Moniram Kathar, Sarlongki Hanse, Siko Teronpi, Ajitsingh Timung, Kaushik Bose, Jaydip Sen

“…Height, weight, waist circumference (WC) and neck circumference (NC) were recorded using standard procedures. The body mass index (BMI kg m−2) was calculated and overweight and obesity were assessed using standard cut-offs. …”
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Risk factors for incomplete excision of cutaneous squamous cell carcinoma: A single-center study performed on 1082 excisions in Northern Italy by S.C. van Helmond, S. Cozzi, B. Breugelmans, D. Codazzi, L. Valdatta, M. Carminati

“…This series included 837 patients who underwent surgical treatment for 1082 primary cutaneous squamous cell carcinoma in the Plastic Surgery Department of the Papa Giovanni XXIII Hospital in Bergamo, Northern Italy between 2012 and 2021. Patient-, procedure-, and tumor characteristics were collected and analyzed. …”
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Institutional learning curve and factors of prolonged operation time of robotic distal pancreatectomy: An analysis of an initial 117 cases by Yuki Hirata, Laura Prakash, Jess Maxwell, Rebecca Snyder, Michael Kim, Hop Tran Cao, Ching‐Wei D. Tzeng, Jefferey E. Lee, Matthew H. G. Katz, Naruhiko Ikoma

“…A multivariable analysis revealed BMI ≥30 in male, the presence of pancreatitis or fibrosis, and the performance of concomitant procedures were associated with extended operation times, while BMI ≥30 in female was not. …”
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JAK2V617F-positive clonal hematopoiesis of indeterminate potential in pregnant women by I. A. Olkhovskiy, J. G. Garber, A. S. Gorbenko, M. A. Stolyar, O. M. Miller, E. S. Kostina, Yu. Yu. Komarovskiy, V. V. Potilitsina

“…The unused samples that remained after the pooling were frozen and stored at –20°C until the end of entire testing procedure. The V617F JAK2 mutation was detected by the real-time allele-specific polymerase chain reaction test.Results. …”
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How to Reduce Delayed Gastric Emptying After Pancreatoduodenectomy: A Systematic Literature Review and Meta-Analysis by Artur Rebelo, MD, Randi Kresse, Yoshiaki Sunami, PhD, Ulrich Ronellenfitsch, MD, Jörg Kleeff, MD, Johannes Klose, MD

“…Although these results are partially based on observational studies, they contribute valuable insights to the current understanding of interventions impacting DGE in these complex procedures.…”
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Serum Amino Acid Profiles in Dogs with a Congenital Portosystemic Shunt by Robert Kyle Phillips, Amanda B. Blake, Michael S. Tivers, Alex Chan, Patricia E. Ishii, Jan S. Suchodolski, Jörg M. Steiner, Jonathan A. Lidbury

“…The Benjamini–Hochberg procedure was used to control for false discovery. …”
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GastroEndoNet: Comprehensive endoscopy image dataset for GERD and polyp detectionMendeley Data by Abu Kowshir Bitto, Md. Hasan Imam Bijoy, Kamrul Hassan Shakil, Aka Das, Khalid Been Badruzzaman Biplob, Imran Mahmud, Syed Md. Minhaz Hossain

“…The dataset consists of 4006 primary images collected following endoscopic procedures, which were augmented using six distinct techniques, expanding the total number of images to 24,036. …”
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A case with massive hemobilia long-term after internal drainage surgery for congenital biliary dilation by Shunryo Minezaki, Takeyuki Misawa, Makoto Watanabe, Hideki Takahashi, Takashi Koenuma, Rie Kondo, Hiroe Toyoda, Kentaro Nemoto, Hiroyuki Tsukayama, Makoto Shibuya, Keita Wada, Keiji Sano, Yasunori Ohta, Satoe Numakura, Yuko Sasajima, Hiroshi Uozaki

“…Based on imaging findings and an understanding of the historical transition of the surgical procedure for CBD, the cause of gastrointestinal bleeding was determined to be rupture of the pseudoaneurysm of the dilated bile duct that remained after IDS. …”
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Evaluation of deep vein thrombosis prophylaxis use in a Northwest Ethiopian medical ward: an observational follow-up study by Samuel Berihun Dagnew, Tilaye Arega Moges, Fisseha Nigussie Dagnew, Abraham Nigussie Assefa, Sisay Sitotaw Anberbr, Adane Tsegaw Geremew, Getu Tesfaw Addis

“…BackgroundDuring hospitalization, a significant number of patients at risk of thromboembolism do not receive prophylaxis, despite established standards and viable procedures for preventing deep vein thrombosis (DVT). …”
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Describing the initial results of a pragmatic, cluster randomized clinical trial to examine the impact of a multifaceted digital intervention for the prevention of type 2 diabetes... by Sofía Mira-Martínez, Sofía Mira-Martínez, Sofía Mira-Martínez, Rocío Zamanillo-Campos, Rocío Zamanillo-Campos, Rocío Zamanillo-Campos, Narges Malih, Narges Malih, Narges Malih, Maria Antonia Fiol-deRoque, Maria Antonia Fiol-deRoque, Maria Antonia Fiol-deRoque, Maria Antonia Fiol-deRoque, Escarlata Angullo-Martínez, Rafael Jimenez, Rafael Jimenez, Maria Jesús Serrano-Ripoll, Maria Jesús Serrano-Ripoll, Maria Jesús Serrano-Ripoll, Maria Jesús Serrano-Ripoll, José Iván Oña, Aina M Galmes-Panades, Aina M Galmes-Panades, Aina M Galmes-Panades, Rocío Gómez-Juanes, Rocío Gómez-Juanes, Rocío Gómez-Juanes, Rocío Gómez-Juanes, Jadwiga Konieczna, Jadwiga Konieczna, Jerónima Miralles, Jerónima Miralles, Dora Romaguera, Dora Romaguera, María Clara Vidal-Thomasa, María Clara Vidal-Thomasa, María Clara Vidal-Thomasa, Joan Llobera-Canaves, Joan Llobera-Canaves, Joan Llobera-Canaves, Joan Llobera-Canaves, Mauro García-Toro, Mauro García-Toro, Mauro García-Toro, Mauro García-Toro, Elena Gervilla-García, Elena Gervilla-García, Catalina Vicens, Catalina Vicens, Catalina Vicens, Oana Bulilete, Oana Bulilete, Oana Bulilete, Oana Bulilete, Juan José Montaño, Juan José Montaño, Margalida Gili, Margalida Gili, Margalida Gili, Margalida Gili, Miquel Roca, Miquel Roca, Miquel Roca, Antoni Colom, Antoni Colom, Ignacio Ricci-Cabello, Ignacio Ricci-Cabello, Ignacio Ricci-Cabello, Ignacio Ricci-Cabello

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Analyzing the practice of medical humanistic care based on a social learning model: mapping the trajectory of the learning dynamic process from the learner’s perspective by Bilu Gu, Yiming Lv, Jiyu Zhu, Xiaoling Sun, Xiaoling Sun, Kun Li

“…Conversely, “backward” behaviors included mechanized procedures and emotional detachment, disregard for privacy and dignity, systemic issues that exacerbate apathy.ConclusionBased on the framework of social learning theory, this study constructs a learning trajectory model of humanistic care to explain the synergistic mechanism between cognitive dimension and environmental system and its two-way shaping of caring practice behavior. …”
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