Bradykinin measurement by liquid chromatography tandem mass spectrometry in subjects with hereditary angioedema enhanced by cold activation by Jinguo Chen, MD, Yunkou Wu, PhD, J. Joanna Yu, MD, PhD, Joseph Chiao, MD, Jing Yu, PhD, Mark D. Scarupa, MD, Lili Wan, PhD, H. Henry Li, MD, PhD

“…The protocol was developed and validated according to the US Food and Drug Administration Bioanalytical Method Validation guidelines. The procedure was optimized on specimen selection, collection, and process, as well as variable controls such as time windows, temperature, and storage conditions. …”
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The influence of male smoking on success rates after IVF/ICSI by S. De Brucker, E. Dhooge, P. Drakopoulos, V. Uvin, S. Mackens, L. Boudry, M. De Vos, V. Vloeberghs, H. Tournaye, M. De Brucker

“…The crude analysis showed higher positive hCG and LBR in smokers (positive hCG 28.34% in nonsmokers vs 33.95% in smokers, p = 0.015; LBR 23.55% in nonsmokers vs 28.64% in smokers, p = 0.019) However after adjustment for relevant confounders (female age, BMI, cause of infertility, number of oocytes retrieved, insemination procedure, number of embryos transferred and day of embryo transfer) the smoking status was not significantly associated with fresh LBR (OR = 1.16, CI = 0.92–1.48; p = 0.2). …”
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INTERNALIZATION OF ANTIMICROBIAL PEPTIDE ACIPENSIN 1 INTO HUMAN TUMOR CELLS by E. S. Umnyakova, I. V. Kudryavtsev, N. A. Grudinina, S. V. Balandin, I. A. Bolosov, P. V. Panteleev, T. A. Filatenkova, D. S. Orlov, E. V. Tsvetkova, T. V. Ovchinnikova, V. N. Kokryakov, O. V. Shamova

“…Capability of acipensin 1 to enter the human erytroleukemia K-562 cells has been investigated for the first time. A biotechnological procedure for producing a recombinant acipensin 1 peptide has been developed. …”
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Epigenetic Alterations in Ovarian Function and Their Impact on Assisted Reproductive Technologies: A Systematic Review by Charalampos Voros, Antonia Varthaliti, Despoina Mavrogianni, Diamantis Athanasiou, Antonia Athanasiou, Aikaterini Athanasiou, Anthi-Maria Papahliou, Constantinos G. Zografos, Vasileios Topalis, Panagiota Kondili, Menelaos Darlas, Sophia Sina, Maria Anastasia Daskalaki, Marianna Theodora, Panos Antsaklis, Georgios Daskalakis

“…Further study is needed to determine the practical applicability of epigenetic biomarkers in predicting ART effectiveness and enhancing patient treatment procedures.…”
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Suitability of a classic wastewater treatment plant to purify the collective effluent of Oum El-Bouaghi City, North East Algeria by Dounia Dib, Miyada Ouanes, Mamoun Fellah, Bachir Khezzani, Dalila Addad, Kenza Kadi, Mounia Ouldjaoui, Abdelkader Khiari, Alejandro Perez-Larios, Benjamin Castañeda-Pimienta, Mariano Aceves-Aldrete, Souren Gregorian, Gamal A. El-Hiti

“…These findings emphasize the efficiency of the biological treatment procedures in purifying the effluent of Oum El-Bouaghi city regarding their domestic character.…”
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OCT-based quantitative predictors of coronary dissection during rotational atherectomy in severe calcified lesions by Yu-he Zhou, Hong-li Zhang, Yang Yang, Mei-qin Wang, Jun Pan, Tian Xu, Pei-na Meng, Fei Ye, Yan-qing Wang

“…BackgroundIn the treatment of coronary calcification by rotational atherectomy (ROTA), guidewire bias is often considered to lead to procedure-associated coronary dissections or perforations. …”
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Multimodal ultrasound radiomics containing microflow images for the prediction of central lymph node metastasis in papillary thyroid carcinoma by Jiangyuan Ben, Jiangyuan Ben, Qiying Yv, Pengfei Zhu, Junhao Ren, Pu Zhou, Guifang Chen, Ying He, Ying He

“…Among the prediction models, the fusion model constructed by Multilayer Perceptron (MLP) algorithm showed the best diagnostic performance, outperforming the other models in both the training cohort (AUC = 0.886) and the testing cohort (AUC = 0.873).ConclusionsThe fusion model based on clinical data and multimodal ultrasound radiomics has better predictive ability and net clinical benefit for CLNM in patients with PTC, confirms the diagnostic value of microflow images for CLNM, and can help to evaluate patients’ preoperative lymph node status and make the correct decision on the surgical procedure.…”
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SYNTHESIS AND STUDY OF THE PHOTOCHROMIC BEHAVIOR OF SUBSTITUTED 6'-NITRO-1,3,3-TRIMETHYL-5-VINYLSPIRO(INDOLINO-2,2'-[2H]CHROMENES) by A. V. Laptev, A. Yu. Lukin, N. V. Belikov, O. V. Demina, S. D. Varfolomeev, V. A. Barachevsky, A. A. Khodonov, V. I. Shvets

“…Compounds 1’’-[6’-nitro-spiropyran-5- yl]-2’’,2’’-dicyanoethylene (4) and E-1’’-[6’-nitro-spiropyran-5-yl]-2’’-cyano-2’’-methoxycarbonylethylene (5) were prepared from initial 1 by reflux in ammonium acetate / glacial acetic acid / benzene mixture with malonodinitrile or methyl 2-cyanoacetate, respectively. The procedure for synthesizing derivatives 2’’,2’’-dimethyl-5’’-(6’-nitrospiropyran-5-yl)methylidene-1’’,3’’-dioxane-4’’,6’’-dion (6) and 5’’,5’’-dimethyl-2’’-(6’-nitro-spiropyran-5-yl)- methylidenecyclohexane-1’’,3’’-dion (7) includes the interaction of Meldrum's acid or dimedone, accordingly, with 5-formyl precursor 1 by heating in ethanol in the presence of piperidine and molecular sieves (to bind the released water). …”
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Hydraulic weakening technology for unsupported top coal at the end of fully mechanized caving face by DONG Haofu, ZHANG Wei, SUN Guangjun, FU Huijian, LONG Zhaoxi, LIU Yang, LIU Dongliang, ZHU Xiaodong

“…This study revealed that high-pressure water jets weakened the mechanical connection between the top coal and coal pillar through impact-induced coal fracturing, cavity creation, and fracture propagation. A standardized procedure of "drilling positioning→high-pressure jet coal breaking→rotational cavity creation" was proposed. …”
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The identification of adenylyl cyclase modulators as potential receptors for 6-nitrodopamine in human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes and their relevan... by Irene Cipollone, Irene Cipollone, Vittoria Monaco, Vittoria Monaco, José Britto-Júnior, Antonio Tiago Lima, Edson Antunes, Andre Sampaio Pupo, Ilaria Iacobucci, Ilaria Iacobucci, Flora Cozzolino, Flora Cozzolino, Maria Monti, Maria Monti, Silvia Parisi, Giuseppina Divisato, Emanuela Cascone, Alfonso De Simone, Angela Corvino, Ferdinando Fiorino, Francesco Frecentese, Vincenzo Santagada, Beatrice Severino, Rosa Sparaco, Pierfrancesco Cinque, Stefania Vertuccio, Giuseppe Caliendo, Gilberto De Nucci, Gilberto De Nucci, Gilberto De Nucci

“…Human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes were harvested and lysed, and following appropriate separation procedures, membrane proteins were incubated with 6-ND-derivatized agarose, centrifuged, and the proteins retained in the agarose eluted with 6-ND (1 mM). …”
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Real-life experience on COVID-19 and seasonal influenza vaccines co-administration in the vaccination hub of the University Hospital of Palermo, Italy by Claudio Costantino, Walter Mazzucco, Arianna Conforto, Livia Cimino, Alessia Pieri, Sara Rusignolo, Nicole Bonaccorso, Floriana Bravatà, Laura Pipitone, Martina Sciortino, Marcello Tocco, Elena Zarcone, Giorgio Graziano, Fabio Tramuto, Carmelo Massimo Maida, Alessandra Casuccio, Francesco Vitale

“…From data obtained, the role of HCPs in accepting and then promoting co-administration of COVID-19 and influenza vaccines is crucial, as well as receiving co-administration in the previous season that represented the main drive for accepting it in the following seasons, supporting safety and effectiveness of this procedure.…”
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“One's life becomes even more miserable when we hear all those hurtful words”. A mixed methods systematic review of disrespect and abuse in abortion care by Meghana Munnangi, Meghana Munnangi, Priya Shreedhar, Regina Gilyan, Hedda Lippus, Hedda Lippus, Dabney P. Evans, Lauren Maxwell, Lauren Maxwell

“…Thirty-eight studies conducted in 20 countries met the inclusion criteria and were included in this review.ResultsThe most frequently observed form of disrespect and abuse, identified in 33 studies, was the failure to meet care standards in providing quality abortion care or PAC, particularly in terms of offering adequate and accurate information to women before, during and after the procedures which was identified in 21 studies. Additionally, stigma was reported in 22 studies, disrespect in 19 studies, discrimination in 10 studies, and verbal, physical and sexual abuse, as well as humiliation and condescension, each in 9 studies. …”
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Isobavachalcone ameliorates Alzheimer disease pathology by autophagy-mediated clearance of amyloid beta and inhibition of NLRP3 inflammasome in primary astrocytes and 5x-FAD mice by Dilpreet Kour, Dilpreet Kour, Parul Khajuria, Parul Khajuria, Kuhu Sharma, Kuhu Sharma, Alpa Sharma, Alpa Sharma, Ankita Sharma, Ankita Sharma, Syed Mudassir Ali, Priya Wazir, P. Ramajayan, P. Ramajayan, Sanghapal D. Sawant, Sanghapal D. Sawant, Sanghapal D. Sawant, Utpal Nandi, Utpal Nandi, Zabeer Ahmed, Zabeer Ahmed, Ajay Kumar, Ajay Kumar

“…To this end, we identified isobavachalcone (IBC), a natural compound with dual pharmacological activity in reducing Aβ plaques and neuroinflammation.Experimental ProcedurePrimary astrocytes were isolated from 3 to 4 days old C57BL/6J mice pups for in-vitro assays, while in-vivo studies were conducted on 5x-FAD mice. …”
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Long-term Outcomes after Second-Generation Cryoballoon Ablation of Atrial Fibrillation and Analysis of Risk Factors Related to Recurrence by Yufan Dai, Chenyuan Wang, Zulu Wang, Ming Liang, Guitang Yang, Zhiqing Jin, Jian Ding, Ping Zhang, Yaling Han

“…Data from 760 consecutive patients in the Department of Cardiology, General Hospital of Northern Theater Command from August 2016 to December 2018 with drug-refractory symptomatic AF undergoing pulmonary vein isolation (PVI) using CB2 were assessed. Procedure-related safety and freedom from AF and atrial flutter/atria tachycardia through 3 years were determined. …”
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Impact of Ablation Targeting Atrial Drivers and Fragmented Potentials on Long‐Standing Persistent Atrial Fibrillation: A Retrospective Study by Yuma Gibo, Morio Ono, Yui Koyanagi, Katsuya Yoshihiro, Soichiro Usumoto, Toshihiko Gokan, Toshitaka Okabe, Naoei Isomura, Mitunori Muto, Masaru Shiigai, Junko Honye, Masahiko Ochiai

“…The primary endpoint was AF recurrence lasting > 30 s occurring ≥ 3 months post‐ablation. Results Procedure times were longer in the driver and fragmented potentials group (166.1 ± 35.9 vs. 140.7 ± 50.4 min, p = 0.01). …”
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Analysis of Implant Osseointegration, Bone Repair, and Sinus Mucosa Integrity Using Bio-Oss^® and Hyaluronic Acid-Polynucleotide Gel (Regenfast^®) in Maxillary... by Hiroyuki Omori, Daniele Botticelli, Erick Ricardo Silva, Samuel Porfirio Xavier, Sérgio Luis Scombatti de Souza, Kaoru Kusano, Shunsuke Baba

“…<b>Background:</b> The combination of polynucleotides and hyaluronic acid with bovine bone grafts in maxillary sinus lift procedures appears to be a promising strategy to enhance bone regeneration. …”
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Progress in Mechanistic Research and the Use of Traditional Chinese Medicine in Treating Malignant Pleural Effusion by Gong F, Cheng Y, Li Y, Fan Q, Qu R, Fan T, Lv Y, Dai J

“…In Western medicine, the treatment of MPE primarily involves procedures such as surgical puncture for drainage, pleural fixation, chemotherapy, and targeted therapy. …”
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Translation, cultural adaptation, and validation of the Concussion Recognition Tool 6 (CRT6™), Sport Concussion Assessment Tool 6 (SCAT6®) and Sport Concussion Office Assessment To... by Veronica Baioccato, Marco Bazo, Irene Leo, Alessandra Simonelli, Andrea Ermolao, Jon S. Patricios, Ruben J. Echemendia, Gavin A. Davis, Valentina Re, Silvia Bressan

“…Methods Based on CISG procedures, we engaged in an 8-step process which sequentially included verbatim translation in Italian, reconciliation of translation and cultural adaptation, back-translation (from Italian into English), appraisal of the back-translation, validation of the refined Italian version, review and adjustment by an expert committee, pilot testing in 12 amateur athletes and document finalization. …”
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Performance of Visual Inspection, ATP, and Microbial Analysis in Evaluating Cleaning and Sanitizing of Surfaces in an Avocado Packing Plant by J.A. Muñiz-Flores, J.A. Pérez-Montaño, M.A. Pineda-Macias, A. Castillo, O. Rodriguez-García

“…This study evaluated the performance of visual inspection, ATP bioluminescence, and microbial analysis as verification tools for C&S procedures in an avocado packing plant, based on surface conditions before and after sanitation. …”
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Platelet aggregation function test for children in order to determine the normative values in different age groups by O. B. Gordeeva, M. S. Karaseva, M. A. Babaikina, N. D. Vashakmadze, M. A. Soloshenko, O. Ya. Smirnova, D. S. Ovchinnikov

“…The high prognostic significance of the Impedance Aggregometry results makes it possible to decide on the drug of choice for patients after thrombosis and intravascular interventions, as well as to prevent bleeding during and after surgical procedures.…”
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