Evaluation of Biocompatible Materials for Enhanced Mesenchymal Stem Cell Expansion: Collagen-Coated Alginate Microcarriers and PLGA Nanofibers by Manuel Jaime-Rodríguez, María Luisa Del Prado-Audelo, Norma Angélica Sosa-Hernández, Dulce Patricia Anaya-Trejo, Luis Jesús Villarreal-Gómez, Ángel Humberto Cabrera-Ramírez, Jesus Augusto Ruiz-Aguirre, Israel Núñez-Tapia, Marek Puskar, Emily Marques dos Reis, Silvia Letasiova, Rocío Alejandra Chávez-Santoscoy

“…Scanning Electron Microscopy (SEM) revealed that bmMSCs adhered not only to the surface but also grew within the porous structure of the alginate microcarriers. Mycoplasma testing confirmed that the bmMSCs were free from contamination. …”
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Metalloproteinases (MMPs) in hypertensive disorders: role, function, pharmacology, and potential strategies to mitigate pathophysiological changes by Soroush Taherkhani, Mohammad Sheibani, Mohammad Sheibani, Ali Mohammadkhanizadeh, Jitka A. I. Virag, Lisandra de Castro Braz, Yaser Azizi

“…MMPs are responsible for degrading various components of the ECM, which is crucial for maintaining vascular structure and function. In hypertensive patients, several MMPs, including MMP-1, MMP-3, and MMP-9, are often found at elevated levels. …”
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Lipid nanoparticle delivery of the CRISPR/Cas9 system directly into the mitochondria of cells carrying m.7778G>T mutation in MtDNA (mt-Atp8) by Kaede Norota, Sen Ishizuka, Misa Hirose, Yusuke Sato, Masatoshi Maeki, Manabu Tokeshi, Saleh M. Ibrahim, Hideyoshi Harashima, Yuma Yamada

“…To overcome the major barrier of the mitochondrial membrane structure, we investigated a strategy to deliver ribonucleoprotein (RNP) directly to mitochondria via membrane fusion using MITO-Porter, a mitochondria-targeting lipid nanoparticle. …”
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Epigenetic modulation with nanosatellite triggers tumoricidal immunity for hepatocellular carcinoma treatment by Ying Qu, Chen Chen, Feifei Sun, Shijia Liu, Guozhi Zhao, Zhongxi Zhao, Chang Liu, Xinyi Jiang, Tao Li

“…Here we report an epi-immune nanosatellite (stEiNS) that co-delivers siRNA targeting the YTH N6-methyladenosine RNA binding protein 1 (YTHDF1) alongside the histone deacetylase IIa inhibitor TMP195, enabling epigenetic reprogramming of HCC tumor cells and M2 macrophages to enhance the immunotherapeutic response. stEiNS assembles size-mismatched nanoparticles via dynamic locks in a satellite-like structure, enabling deep tissue penetration. Knockdown of YTHDF1 by stEiNS in HCC cells, along with stEiNS-driven antitumor macrophage phenotype induction, intensifies macrophages-cytotoxic T lymphocytes interactions with tumor cells. stEiNS suppresses TNF/NF-κB signaling in tumor cells to inhibit CCL2-driven recruitment of myeloid-derived suppressor cells while activating the IFNγ/STAT1 pathway in M2-phenotype macrophages to promote their polarization toward an M1 phenotype. …”
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Co-exposure to inhaled tungsten particles and low-dose gamma rays: neurotoxicological outcome in rats by Théo Fréchard, Florence Bachelot, Véronique Ménard, Chloé Brizais, Léo Macé, Christelle Elie, Nicolas Cailler Gruet, Théo Teulade, Chloé Havet, Frédéric Voyer, Imène Garali, Céline Gloaguen, François Gensdarmes, Dmitry Klokov, Chrystelle Ibanez

“…The effects persisted at 28 days and were brain structure specific. Biodistribution of tungsten showed that both local and systemic effects might be involved. …”
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Development of a duplex real-time PCR method for the detection of influenza C and D viruses by Letian Zhang, Meng Lu, Jiaxuan Lu, Ningning Wang, Zhongzhou Pan, Shuo Su

“…Abstract Influenza viruses are major respiratory pathogens known to infect human and a variety of animals and are widely prevalent worldwide. Genome structure of influenza D virus (IDV) is identical to that of influenza C virus (ICV), and phylogenetic analyses suggest that IDV and ICV share a common ancestry and high homology. …”
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Spatially distributed gradient-based calibration and parametric sensitivity of a spatialized hydrological model over 235 French catchments by Pierre-André Garambois, François Colleoni, Ngo Nghi Truyen Huynh, Ahmad Akhtari, Ngoc Bao Nguyen, Apolline El-Baz, Maxime Jay-Allemand, Pierre Javelle

“…New hydrological insights for the regions:: Under varying hydrological conditions, the GR4-like structure outperforms a simpler 3-parameter model in terms of robustness and accuracy. …”
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MiR‐182‐3p targets TRF2 and impairs tumor growth of triple‐negative breast cancer by Roberto Dinami, Luca Pompili, Eleonora Petti, Manuela Porru, Carmen D'Angelo, Serena Di Vito, Angela Rizzo, Virginia Campani, Giuseppe De Rosa, Alejandra Bruna, Violeta Serra, Miguel Mano, Mauro Giacca, Carlo Leonetti, Gennaro Ciliberto, Madalena Tarsounas, Antonella Stoppacciaro, Stefan Schoeftner, Annamaria Biroccio

“…Abstract The telomeric repeat‐binding factor 2 (TRF2) is a telomere‐capping protein that plays a key role in the maintenance of telomere structure and function. It is highly expressed in different cancer types, and it contributes to cancer progression. …”
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An engineered M2 macrophage-derived exosomes-loaded electrospun biomimetic periosteum promotes cell recruitment, immunoregulation, and angiogenesis in bone regeneration by Zhuohao Wen, Shuyi Li, Yi Liu, Xueyan Liu, Huiguo Qiu, Yuejuan Che, Liming Bian, Miao Zhou

“…The electrospun membrane is fabricated using poly(ε-caprolactone) (core)-periosteal decellularized extracellular matrix (shell) fibers via coaxial electrospinning, to mimic the fibrous structure, mechanical property, and tissue microenvironment of natural periosteum. …”
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Intelligent Identification and Prediction of Roof Deterioration Areas Based on Measurements While Drilling by Jing Wu, Zhi-Qiang Zhao, Xiao-He Wang, Yi-Qing Wang, Xiao-Xiang Wei, Zhi-Qiang You

“…The results indicate that these drilling parameters effectively characterize variations in rock structure and strength, with distinct signal features observed particularly in roof deterioration zones. …”
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Application of the Biorefinery Concept in the Processing of Crambe (<i>Crambe abyssinica</i> Hochst) Seed Defatted Meal in a Pressurized Medium by Camila da Silva, Jefferson Alessandro Schmitz, Djéssica Tatiane Raspe, Natália Stevanato, Jaqueline Hoscheid, Marcelino Luiz Gimenes, Beatriz Cervejeira Bolanho Barros, Lúcio Cardozo-Filho

“…The valorization of byproducts such as defatted meal (DM) is essential for the implementation of a biorefinery structure and can be achieved through the application of emerging technologies, such as pressurized liquid extraction. …”
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Developing and validating an artificial intelligence ethical awareness scale for secondary and university students: Cultivating ethical awareness through problem-solving with artif... by Siu Cheung Kong, Jinyu Zhu

“…Confirmatory factor analysis (CFA) supported this three-factor structure, with nine items retained. Multigroup CFA results demonstrated strong measurement invariance across gender, education level, and programming knowledge. …”
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Towards Hazard Analysis Result Verification for Autonomous Ships: A Formal Verification Method Based on Timed Automata by Xiang-Yu Zhou, Shiqi Jin, Yang Mei, Xu Sun, Xue Yang, Shengzheng Nie, Wenjun Zhang

“…The results indicate that the timed automata network model for remotely controlled ships, based on the control structure, has no deadlocks and operates correctly, which demonstrates its practicability and effectiveness. …”
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Prediction of the viscoelastic parameters of asparagus and optimization of the harvesting robot gripping force based on a back-propagation neural network by Yuan Fahui, Wang Xiaochan, Shi Yinyan, Wang Lei, Zhang Xiaolei, Lu Wei, Zheng Enlai

“…The creep behavior of asparagus was described using the Burgers model, and the corresponding viscoelastic parameters were obtained. The topological structure and transfer function of the prediction model were determined through repeated training, and the gripping force range was determined based on conditions producing no damage and stable gripping. …”
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Adsorption of Uranium(VI) from Aqueous Solution by Modified Rice Stem by Zhang Xiao-teng, Jiang Dong-mei, Xiao Yi-qun, Chen Jun-chang, Hao Shuai, Xia Liang-shu

“…The adsorption of uranium can change the morphology and the structure characteristics of the modified rice stem through interaction with the adsorption sites, such as O-H, C=O, Si=O, and P-O on the surface.…”
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Single-cell sequencing reveals dynamic cell development trajectories in two kiwifruit (Actinidia chinensis) genotypes with contrasting cold resistance by Miaomiao Lin, Zhenzhen Zhang, Qina Zhang, Shihang Sun, Yukuo Li, Mingyu Liu, Yihang Li, Congcong Li, Jinbao Fang, Xiujuan Qi

“…Abstract Kiwifruit (Actinidia Lindl.) is a valuable fruit tree species, with its cold resistance being influenced by leaf structure and various cell types. Despite significant progress in understanding the cold stress response during the last decade, the mechanisms underlying the formation of distinct cold-resistant cell types in kiwifruit, particularly at the single-cell level, remain poorly understood. …”
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Identification and Characterization of Novel Inhibitors of Human Poly(ADP-Ribose) Polymerase-1 by Ibrahim Morgan, Robert Rennert, Robert Berger, Ahmed Hassanin, Mehdi D. Davari, Daniela Eisenschmidt-Bönn, Ludger A. Wessjohann

“…Nonetheless, this new scaffold demonstrated a five-fold higher PARP inhibitory activity at the enzyme level compared to the core structure of olaparib (OLP), phthalazin-1(2<i>H</i>)-one.…”
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Sampling strategies for digestive system flora studies: current research and perspectives by Xiaobo Liu, Xia Cheng, Ziye Gao, Jun Pan, Shizhen Luo, Pei Liu, Hui Wen, Shu Jin

“…The focus of this review is on microbial community research, particularly the use of high-throughput sequencing to compare different samples of the same organization, as well as the impact of different sampling methods on microbial community structure of the same sample. Results A diverse array of microbiological samples are available for study, including saliva, endoscopic biopsies, luminal mucosa, luminal fluid, feces, bile, and surgically excised tissues. …”
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Flexible loop-closing scheme for distribution network based on cascaded power electronic transformer with multiple-AC-ports by LI Qian, ZHAO Junyu, WANG Shengnan, XIE Sizhe, XU Yonghai, WEN Xian

“…This paper proposes an AC flexible loop-closing scheme based on the multiple-AC-ports cascaded H-bridge type power electronic transformer (MAC-CHB-PET). The structure of MAC-CHB-PET is analyzed. The internal mechanism of the impulse current and steady-state current generated during loop-closing are analyzed, and a power command value calculation method based on MAC-CHB-PET loop-closing regulation is proposed based on the selected characteristic factors. …”
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Internalized polystyrene nanoplastics trigger testicular damage and promote ferroptosis via CISD1 downregulation in mouse spermatocyte by Jing Lv, Guangyu Liu, Ziqi Wang, Jueshun Zhang, Yuanyou Li, Yifan Wang, Ning Liu, Shayakhmetova Altyn, Zhongliang Jiang

“…These changes ultimately result in disturbances to mitochondrial structure and function. In terms of mechanism, pioglitazone, a drug that stabilizes CISD1, has been demonstrated to mitigate ferroptosis induced by NCOA4-mediated ferritinophagy in GC-2 cells. …”
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