Evaluation of the Efficacy of the HIV Ab/Ag Combi Chemiluminescence Immunoassay (CLIA) Method in the Diagnosis and Follow-up of HIV Infections

BACKGROUND: Diagnosis of human immunodeficiency virus (HIV) infection is important in detection of infected individuals and treatment planning. Serological methods, especially fourth-generation tests are used for screening and can detect both HIV-specific antibodies and p24 antigen. AIM: This study...

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Main Authors: Neşe İnal, Bengü Sayar, Alpaslan Alp, Özgen Köseoğlu Eser
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Journal of Global Antimicrobial Resistance
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Online Access:http://www.sciencedirect.com/science/article/pii/S221371652400287X
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author Neşe İnal
Bengü Sayar
Alpaslan Alp
Özgen Köseoğlu Eser
author_facet Neşe İnal
Bengü Sayar
Alpaslan Alp
Özgen Köseoğlu Eser
author_sort Neşe İnal
collection DOAJ
description BACKGROUND: Diagnosis of human immunodeficiency virus (HIV) infection is important in detection of infected individuals and treatment planning. Serological methods, especially fourth-generation tests are used for screening and can detect both HIV-specific antibodies and p24 antigen. AIM: This study was investigated the efficacy of Maglumi-HIV Chemiluminescence Immunoassay test for detection of HIV Ab/Ag in patients’ plasma samples having low/high positive and negative HIV-RNA. METHODS: Plasma samples of 179 patients were collected between January 2023 and June 2024. HIV-RNA Rt-PCR (Abbott,USA) and Maglumi-HIV assay (Snibe Diagnostics, Shenzhen, China) were performed simultaneously in the same plasma samples of patient. RESULTS: By Rt-PCR 54 high positive (10²-10⁷ IU/mL) and 32 low positive (40-10² IU/mL) HIV-RNA results were obtained. HIV-RNA was not detected in 93 samples by Rt-PCR. All of low positive samples and 53 high positive samples were also detected as positive by CLIA. Only one sample with high HIV-RNA positivity was found to be negative by CLIA. HIV Ab/Ag was found to be positive in the 20 samples out of 93 HIV-RNA negative samples. In the clinical analysis of these 20 patients, it was found out that all of them were diagnosed with HIV and were in remission being on anti-retroviral therapy which significantly reduced viral load (Table 1). CONCLUSION: In screening of HIV infections, the HIV Ab/Ag CLIA and HIV-RNA Rt-PCR along with a thorough evaluation of the patient's clinical findings should be used together. The Maglumi-HIV Ab/Ag assay may be used as an alternative screening method for the detection of HIV infection.
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spelling doaj-art-ffcb0e1bdf1e40bfac5842a6e7f59a602024-12-27T04:08:30ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-12-013935Evaluation of the Efficacy of the HIV Ab/Ag Combi Chemiluminescence Immunoassay (CLIA) Method in the Diagnosis and Follow-up of HIV InfectionsNeşe İnal0Bengü Sayar1Alpaslan Alp2Özgen Köseoğlu Eser3Department of Medical Microbiology, Ağrı Training and Research Hospital, AğrıDepartment of Medical Microbiology, Hacettepe University Faculty of Medicine, AnkaraDepartment of Medical Microbiology, Hacettepe University Faculty of Medicine, AnkaraDepartment of Medical Microbiology, Hacettepe University Faculty of Medicine, AnkaraBACKGROUND: Diagnosis of human immunodeficiency virus (HIV) infection is important in detection of infected individuals and treatment planning. Serological methods, especially fourth-generation tests are used for screening and can detect both HIV-specific antibodies and p24 antigen. AIM: This study was investigated the efficacy of Maglumi-HIV Chemiluminescence Immunoassay test for detection of HIV Ab/Ag in patients’ plasma samples having low/high positive and negative HIV-RNA. METHODS: Plasma samples of 179 patients were collected between January 2023 and June 2024. HIV-RNA Rt-PCR (Abbott,USA) and Maglumi-HIV assay (Snibe Diagnostics, Shenzhen, China) were performed simultaneously in the same plasma samples of patient. RESULTS: By Rt-PCR 54 high positive (10²-10⁷ IU/mL) and 32 low positive (40-10² IU/mL) HIV-RNA results were obtained. HIV-RNA was not detected in 93 samples by Rt-PCR. All of low positive samples and 53 high positive samples were also detected as positive by CLIA. Only one sample with high HIV-RNA positivity was found to be negative by CLIA. HIV Ab/Ag was found to be positive in the 20 samples out of 93 HIV-RNA negative samples. In the clinical analysis of these 20 patients, it was found out that all of them were diagnosed with HIV and were in remission being on anti-retroviral therapy which significantly reduced viral load (Table 1). CONCLUSION: In screening of HIV infections, the HIV Ab/Ag CLIA and HIV-RNA Rt-PCR along with a thorough evaluation of the patient's clinical findings should be used together. The Maglumi-HIV Ab/Ag assay may be used as an alternative screening method for the detection of HIV infection.http://www.sciencedirect.com/science/article/pii/S221371652400287XHIVHIV RNAHIV Ab/Agchemiluminescence immunoassayReal-time PCR
spellingShingle Neşe İnal
Bengü Sayar
Alpaslan Alp
Özgen Köseoğlu Eser
Evaluation of the Efficacy of the HIV Ab/Ag Combi Chemiluminescence Immunoassay (CLIA) Method in the Diagnosis and Follow-up of HIV Infections
Journal of Global Antimicrobial Resistance
HIV
HIV RNA
HIV Ab/Ag
chemiluminescence immunoassay
Real-time PCR
title Evaluation of the Efficacy of the HIV Ab/Ag Combi Chemiluminescence Immunoassay (CLIA) Method in the Diagnosis and Follow-up of HIV Infections
title_full Evaluation of the Efficacy of the HIV Ab/Ag Combi Chemiluminescence Immunoassay (CLIA) Method in the Diagnosis and Follow-up of HIV Infections
title_fullStr Evaluation of the Efficacy of the HIV Ab/Ag Combi Chemiluminescence Immunoassay (CLIA) Method in the Diagnosis and Follow-up of HIV Infections
title_full_unstemmed Evaluation of the Efficacy of the HIV Ab/Ag Combi Chemiluminescence Immunoassay (CLIA) Method in the Diagnosis and Follow-up of HIV Infections
title_short Evaluation of the Efficacy of the HIV Ab/Ag Combi Chemiluminescence Immunoassay (CLIA) Method in the Diagnosis and Follow-up of HIV Infections
title_sort evaluation of the efficacy of the hiv ab ag combi chemiluminescence immunoassay clia method in the diagnosis and follow up of hiv infections
topic HIV
HIV RNA
HIV Ab/Ag
chemiluminescence immunoassay
Real-time PCR
url http://www.sciencedirect.com/science/article/pii/S221371652400287X
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